• 한국전자파학회논문지
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• 제27권5호
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• pp.463-470
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• 2016

본 논문에서는 다중 편파(polarimetry) 데이터를 이용한 산란점(scattering center) 추출 알고리즘을 소개하고자 한다. 산란파 계산을 위해 상용 툴인 VIRAF(virtual aircraft framework)의 물리 광학법(Physical Optics: PO)/물리광학 회절이론(Physical Theory of Diffraction: PTD)을 사용하여 표적 표면과 모서리에 의한 산란을 각각 계산하였다. 또한, 단위 변환(unitary transformation)을 이용하여 선형 기저(linear basis) 기반 4-채널 데이터를 수평/수직-좌원형 기저(horizontal/vertical-circular basis) 2-채널 데이터로 변환하였고, 그 결과 데이터를 코히런트하게 압축할 수 있었다. 스펙트럼 추정 방법(spectral estimation technique)에 하나인 2차원 RELAX 알고리즘을 사용하여 산란점(scattering center) 추출을 하였고, 편파 방향과 관측각도 변화에 따른 산란현상을 각각 분석하였다.

• 생명과학회지
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• 제24권1호
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• pp.14-19
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• 2014

Kinesin-1은 2개의 장쇄(KHCs, 또는 KIF5s)와 2개의 단쇄(KLCs)가 결합한 복합체로 되어 있다. 본 연구에서 효모 two-hybrid system을 이용하여 중추신경계의 신경세포에서 주로 발현되는 KIF5A와 결합하는 단백질을 탐색한 결과 phosphatidylinositol-3,5-bisphosphate ($PI(3,5)P_2$)의 5번 위치 인산을 제거하는 탈인산화효소 Fig4(Sac3)를 분리하였다. KIF5A는 Fig4의 C-말단과 결합함을 효모 two-hybrid assay로 확인하였다. Fig4는 KIF5A의 C-말단과 결합하지만, 두 개의 다른 장쇄인 KIF5B와 KIF5C 그리고 KLC1와는 결합하지 않았다. 단백질 간 결합을 glutathione S-transferase pull-down assay와 공동면역침강으로 추가 검증하였다. 생쥐의 뇌 파쇄액을 KIF5A 항체로 면역 침강한 결과 Fig4가 같이 침강하였다. 이러한 결과들은 kinesin-1이 Fig4와 결합한 단백질 복합체 혹은 운반체를 세포 내에서 운반함을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1401-1411
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• 2018

The serine-threonine kinase AKT plays a pivotal role in tumor progression and is frequently overactivated in cancer cells; this protein is therefore a critical therapeutic target for cancer intervention. We aimed to identify small molecule inhibitors of the pleckstrin homology (PH) domain of AKT to disrupt binding of phosphatidylinositol-3,4,5-trisphosphate (PIP3), thereby downregulating AKT activity. Liposome pulldown assays coupled with fluorescence spectrometry were used to screen flavonoids for inhibition of the AKT PH-PIP3 interaction. Western blotting was used to determine the effects of the inhibitors on AKT activation in cancer cells, and in silico docking was used for structural analysis and optimization of inhibitor structure. Several flavonoids showing up to 50% inhibition of the AKT PH-PIP3 interaction decreased the level of AKT activation at the cellular level. In addition, the modified flavonoid showed increased inhibitory effects and the approach would be applied to develop anticancer drug candidates. In this study, we provide a rationale for targeting the lipid-binding domain of AKT, rather than the catalytic kinase domain, in anticancer drug development.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2018년도 추계학술대회
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• pp.444-447
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• 2018

Baculovirus 발현 시스템은 박테리아 발현 시스템, 특히 복잡한 번역 후 변형을 필요로 하는 것과 비교하여 다량의 재조합 단백질을 생성하는 빠르고 비용 효율적인 방법을 포함하는 많은 알려진 장점을 갖는다. 특히 재조합 baculovirus는 광범위한 포유류 세포 유형에서 벡터를 전달하고 재조합 단백질을 발현 할 수 있다. 본 연구에서는 pcDNA3.1로부터 재구성된 baculovirus 벡터를 사용하였는데 이 벡터는 cytomegalovirus (CMV) 프로모터, uroplakin II promoter, polyhedron promoter, 수포 구내염 바이러스 G (VSVG), 녹색 형광 단백질 (EGFP), 단백질 전달 도메인 (PTD) 유전자 등 다양한 유전자들로 재조합 되어 개발되었다. 이러한 재구성 된 벡터를 다양한 세포 및 세포주에 감염시켰다. 이렇게 개발된 baculovirus 벡터는 재조합된 유전자들의 전이성 및 발현성을 기존의 일반적인 벡터와 비교하여 분석하였다. 본 연구결과로 이렇게 개발된 baculovirus 벡터는 기존의 대조군 벡터보다 전이성 및 발현성면에서 더 높은 효율을 갖는다는 것을 확인하였다.

• 한국정밀공학회지
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• 제29권2호
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• pp.129-138
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• 2012

In this study, three types of large scale multi-tasking machine tools together with core technologies involved have been developed and introduced; a multi-tasking machine tool for large scale marine engine crankshafts, a multi-tasking vertical lathe for windmill parts, and a large scale 5-axis machine tool of gantry type. Several special purpose devices has been necessarily developed for the purpose of handling and machining big and heavy workpieces accurately, such as PTD (Pin Turning Device) with revolving ring spindle for machining eccentric crankshaft pins, hydrostatic rotary table and steady rest for supporting and resting heavy workpieces, and 2-axis automatic swiveling head for high-quality free surface machining. Core technologies have been also developed and adopted on their detail design stage; 1) structural design optimization with FEM structural analysis, 2) theoretical hydrostatic analysis for the PTD and rotary table bearings, 3) box-in-box type cross-rail and octagonal ram design to secure machine rigidity and accuracy, 4) constant spindle rpm control against gravitational torque due to unbalanced workpiece.

• Journal of Electrical Engineering and Technology
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• 제6권6호
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• pp.824-828
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• 2011

Transmission line analysis of the surface a cumulation layer in injection-enhanced gate transistor (IEGT) is presented for the first time, based on per-unit-length resistance and conductance of the surface layer beneath the gate of IEGT. Lateral electric field on the accumulation layer surface, as well as the electron current injected into the accumulation layer, is governed by the well-known wave equation, and decreases as an exponential function of the lateral distance from the cathode. Unit-length resistance and conductance of the layer are expressed in terms of the device parameters and the applied gate voltage. Results obtained from the experiments are consistent with the numerical simulations.

• 산업기술연구
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• 제40권1호
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• pp.1-6
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• 2020

In the process of protein transcription and translation, various protein complexes bind to DNA, and all processes are precisely controlled. Among the proteins constituting this complex, a peptide derived from eukaryotic initiation factor (eIF) 2 was synthesized. In addition, in order to increase the efficiency of transduction of this peptide into cells, peptides with polyarginine, one of the protein transduction domains (PTD), were synthesized. Cell growth inhibition was confirmed in HER2 positive breast cancer (SK-Br-3) and HER2 negative breast cancer (MDA-MB-231), and cardiomyocytes (H9c2). The peptide with polyarginine had high transduction efficiency in all cells, and had excellent cancer cell growth inhibitory effects. The peptide used in this study might be useful peptide therapeutics for the treatment of cancer through future research.

• 한국정밀공학회지
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• 제22권7호
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• pp.94-101
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• 2005

This work proposes a new method for describing the hysteresis non-linearity of a piezoelectric actuator. The hysteresis behaviour of piezoelectric actuators, including the minor loop trajectory, are modeled by geometrical relationship between a reference major loop and its minor loops. This hysteresis model is transformed into inverse hysteresis model in order to output compensated voltage with regard to the given input displacement. A feedforward neural network, which is trained by a feedback PID control module, is incorporated to the inverse hysteresis model to compensate unknown dynamics of the piezoelectric system. To show the feasibility of the proposed feedforward-feedback controller, some experiments have been carried out and the tracking performance was compared to that of simple PTD controller.

• 한국통신학회논문지
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• 제28권11C호
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• pp.1062-1069
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• 2003

본 논문에서는 요구된 성능을 만족시키는 강인한 최적 PID 제어기 설계를 위한 새로운 루프 형성 기법을 제안 하고자 한다. 설계하고자 하는 PID 제어기의 영점들을 플랜트의 최대 극점에 근접하도록 가중치 요소 Q와 R을 선택하면 루프 형상이 고주파수 영역의 센서잡음 장벽으로부터 최대한 멀어지게 된다. 2차 시스템에 대하여 강인한 최적 PTD 제어기 설계가 가능한 새로운 루프 형성 기법은 LQR 방법에서 발전되었다.

• 음성과학
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• 제10권4호
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• pp.229-239
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• 2003

The purposes of this study are; to investigate the swallowing mechanism of stroke patients with different consistencies through the modified barium swallowing(MBS); and to establish preliminary data on the differences in swallowing durations. 4 different kinds of consistency-water, nectar, pudding, and crackers-were given to 6 stroke patients and their swallowing durations recorded through the fluoroscopy were measured in 1/100 second units. The results show that first swallowing time(FST), the oral preparatory duration(OPD), and the pharyngeal response duration(PRD) were delayed in swallowing thicker consistencies. However, water exhibited delayed oral and pharyngeal phase relative to its consistency and 50% of subjects showed oral loss of water. The relationship between consistencies and swallowing durations and the clinical issues on the stroke population were discussed.