• Title/Summary/Keyword: PSf

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A Quantitative Study of the Quality of Deconvolved Wide-field Microscopy Images as Function of Empirical Three-dimensional Point Spread Functions

  • Adur, Javier;Vicente, Nathalie;Diaz-Zamboni, Javier;Izaguirre, Maria Fernanda;Casco, Victor Hugo
    • Journal of the Optical Society of Korea
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    • v.15 no.3
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    • pp.252-263
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    • 2011
  • In this work, for the first time, the quality of restoration in wide-field microscopy images after deconvolution was analyzed as a function of different Point Spread Functions using one deconvolution method, on a specimen of known size and on a biological specimen. The empirical Point Spread Function determination can significantly depend on the numerical aperture, refractive index of the embedding medium, refractive index of the immersion oil and cover slip thickness. The influence of all of these factors is shown in the same article and using the same microscope. We have found that the best deconvolution results are obtained when the empirical PSF utilized is obtained under the same conditions as the specimen. We also demonstrated that it is very important to quantitatively check the process' outcome using several quality indicators: Full-Width at Half-Maximum, Contrast-to-Noise Ratio, Signal-to-Noise Ratio and a Tenengrad-based function. We detected a significant improvement when using an indicator to measure the focus of the whole stack. Therefore, to qualitatively determinate the best deconvolved image between different conditions, one approach that we are pursuing is to use Tenengrad-based function indicators in images obtained using a wide-field microscope.

Influence of truncated gaussian beam on read-out signal in optical disc (단락된 가우스 광이 광학 디스크 재생 신호에 미치는 영향)

  • 박성종;정창섭
    • Korean Journal of Optics and Photonics
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    • v.7 no.4
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    • pp.434-439
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    • 1996
  • To investigate influence of the incident beams which have the truncated Gaussian amplitude and of the shapes of bump on read-out signal is an optical disc, and the point spread function on bump, the scalar diffraction theory is used in this paper. We consider the truncated Gaussian amplitudes which are $\sigma$=0, 0.5, 1.5, and 2.5, the height of bump which is given by $n{\Delta}_0={\lambda}/4$, and the phase height of bump which is then given by ${\Phi}_0={\pi}$. We also consider the shapes of the bump which are a rectangular shape, a frustoconical shape, and a conical shape. It is shown that as the truncation of incident beam reduces the radius of central spot on bump decreases, the maximum value of read-out signal increases, and that the size of bump decreases. From these results, we get better read-out signal and the reduced cross-talk in optical disc when the truncation of incident beam reduces. Therefore a laser beam having less truncated Gaussian amplitude may useful for an actual optical disc.

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The Evaluation of Image Quality According to the Change of Reconstruction Algorithm of CT Images (재구성 알고리즘 변화에 따른 CT 영상의 화질 평가)

  • Han, Dong-Kyoon;Park, Kun-Jin;Ko, Shin-Kwan
    • Korean Journal of Digital Imaging in Medicine
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    • v.12 no.2
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    • pp.127-132
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    • 2010
  • In this study, the correlation among the changes of Modulation Transfer Function(MTF) in the noise and high-contrast resolution and the change of Contrast to noise ratio(CNR) in the low-contrast resolution will be examined to investigate the estimation of image quality according to the type of algorithms. The image data obtained by scanning American Association of Physicists in Medicine(AAPM) phantom was applied to each algorithm and the exposure condition of 120 kVp, 250 mAs, and then the CT number and noise were measured. The MTF curved line of the high-contrast resolution was calculated with Point Spread Function(PSF) by using the analysis program by Philips, resulting in 0.5 MTF, 0.1 MTF and 0.02 MTF respectively. The low-contrast resolution was calculated with CNR and the uniformity was measured to each algorithm. Since the measurement value for the uniformity of the equipment was below ${\pm}$ 5 HU, which is the criterion figure, it was found to belong to the normal range. As the algorithm got closer from soft to edge, the standard deviation of CT number increased, which indicates that the noise increased as well. As for MTF, 0.5 MTF, 0.1 MTF and 0.02 MTF were all sharp algorithms, and as the algorithm got closer from soft to edge, it was possible to distinguish more clearly with the naked eye. On the other hand, CNR gradually decreased, because the difference between the contrast hole CT number and the acrylic CT number was the same while the noise of hole increased.

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MTF measuring method of TDI camera electronics

  • Kim, Young-Sun;Kong, Jong-Pil;Heo, Haeng-Pal;Park, Jong-Euk;Yong, Sang-Soon;Choi, Hae-Jin
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.540-543
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    • 2007
  • The modulation transfer function (MTF) in a camera system is a measurement of how well the system will faithfully reproduce the original scene. The electro-optical camera system consists of optics, an array of pixels, and an electronics which is related to the image signal chain. The system MTF can be cascaded with each element's MTF in the frequency domain. That is to say, the electronics MTF including the detector MTF can be recalculated easily by the acquired system MTF if the well-known test optics is used in the measuring process. A Time-Delay and Integration (TDI) detector can make a signal increase by taking multiple exposures of the same object and adding them. It can be considered the various methods to measure the MTF of the TDI camera system. This paper shows the actual and practical MTF measuring methods for the detector and electronics in the TDI camera. The several methods are described according to the scan direction as well as the TDI stages such as the single line mode and the multiple-lines mode. The measuring is performed in the in the static condition or dynamic condition to get the point spread function (PSF) or the line spread function (LSF). Especially, the dynamic test bench is used to simulate on track velocity to synchronize with TDI read out frequency for the dynamic movement.

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Cultural characteristics of fungal species associated with deterioration or foxing of paper and chemical removal (종이변색균류의 배양적 특성 및 화학적 방법에 의한 변색제거)

  • Jo, Seong-Eun;Kim, Yong-Tae;Jeong, So-Yeong;Jo, Byeong-Muk;Lee, Jong-Gyu
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2009.04a
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    • pp.295-303
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    • 2009
  • The annals of Joseon dynasty, especially the volumes of King SeJong(1418-1450 A.D.), were heavily deteriorated by fungi. Investigations on the deteriorating and foxing fungi were carried out. Fungal structures on the beeswax, which were coated on the both side of Han-Ji, were suspected to be involved in the deterioration, and were observed by SEM. Isolation and culturing of these fungi were tried by scrubing swab samples and placing on the artificial media. Culture-independent approaches were used to identify the fungal strains associated with damages of beeswax and foxing of the paper by the analyses based on DNA sequences data from the specific ITS region of rDNA regions. In addition, well-known paper staining fungi(PSF), i.e., Aspergillus terreus var. terreus, Fusarium oxysporum, Chaetomium globosum, Cladosporium cladosporioides, and Alternaria solani, were compared in the mycelial growth and stain on beeswax and papers under different environmental conditions (temperature, light, moisture, etc). Fungal strains isolated from the air samples in the storage room and shelves were identified as Irpex sp., Arthrinium sacchari, Cladosporium tenuissimum, Aspergillus sclerotiorum, Sistotrema brinkmannii, and Hypoxylon bovei var. microsporum The isolated strains were compared in growth and stain patterns on beeswax and papers(Han-Ji, Hwa-Ji, and Yang-Ji) whether these can cause damage or foxing on the annals or not.

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A Study on the Bundle-type Scintillating Fiber and Tapered Scintillator Radiation Sensors for Monitoring of Radioactive Wastes Disposal Sites (방사성폐기물 처분장 감시를 위한 다발형 광섬유 센서 및 Tapered 섬광체 방사선 센서에 관한 연구)

  • 김계홍;박재우
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2003.11a
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    • pp.233-238
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    • 2003
  • Several types of gamma-ray sensors were constructed by packing different numbers of fibers into aluminum tubes, and tested to detect the $^137Cs$ gamma ray. It has been found from this investigation that tapered fibers can be more efficient to collect the lights produced inside the sensor and transfer them into the transmitting fiber in order to predict the functioning of the tapered fiber, tapered plastic scintillators, composed of polystyrene with minute amount of dPOPOP and PPO or dPBD, were fabricated and tested for the detection of gamma rays from 1.0 1.5 3.0 5.0 ${\mu}Ci$ $^137Cs$ sources, and the pulse hight spectrum and the relationship between the radioactivity and the total counts are analyzed. It has been found from this study that the tapered scintillating optical fiber, if manufactured, can be practically applied to the development of gamma-ray sensors which can be deployed In ${\mu}Ci$-level radiation fields

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The Identification of Blur Extent from Space-variant Motion Blurred Image (지역적으로 다양한 모션 블러가 발생된 이미지로부터 블러의 크기를 추출하는 기법)

  • Yang, Hong-Taek;Hwang, Joo-Youn;Paik, Doo-Won
    • Journal of Internet Computing and Services
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    • v.8 no.6
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    • pp.169-180
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    • 2007
  • When an image is captured, motion blurs are caused by relative motion between the camera and the scene, In the case of the camera is moving, the extents of the motion blur are spatially variant according to distances from the camera to the objects. Although the camera is fixed, the extents of the motion blur are spatially variant according to various speeds of the moving objects. Unexpected blur effect very often degrades the quality of the image and it needs to be restored, To restore the spatially variant blurred image, each of the point spread function (PSF) should be identified, In this paper, we propose a new method for the identification of blur extent locally from the image in which the spatially variant motion blur is caused. Experiment shows that the proposed method identifies blur extent well.

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A Study on the Performance of Causal Links between Error Causes: Application to Railroad Accident Cases

  • Kim, Dong San;Yoon, Wan Chul
    • Journal of the Ergonomics Society of Korea
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    • v.32 no.6
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    • pp.535-540
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    • 2013
  • Objective: The aim of this study is to evaluate the effectiveness and efficiency of causal links between various error causes in human error analysis. Background: As finding root causes of human error in safety-critical systems is often a cognitively demanding and time-consuming task, it is particularly necessary to develop a method for improving both the quality and efficiency of the task. Although a few methods such as CREAM have suggested causal linking between error causes as a means to enhance the quality and efficiency of human error analysis, no published research to date has evaluated the performance of the causal links. Method: The performance of the CREAM links between error causes were evaluated with 80 railway accident investigation reports from the UK. From each report, errorneous actions of operators were derived, and for each error, candidate causes were found by following the predefined links. Two measures, coverage and selectivity, were used to evaluate the effectiveness and efficiency of the links, respectively. Results: On average, 96% of error causes actually included in the accident reports were found by following the causal links, and among the total of 121 possible error causes, the number of error causes to be examined further was reduced to one-tenth on average. As an additional result of this work, frequent error causes and frequently used links are provided. Conclusion: This result implies that the predefined causal links between error causes can significantly reduce the time and effort required to find the multiple levels of error causes and their causal relations without losing the quality of the results. Application: The CREAM links can be applied to human error analysis in any industry with minor modifications.

Development of a Comprehensive Model for Human Error Prevention in Industrial Fields (산업현장의 휴먼에러 예방을 위한 통합적 분석모델의 개발)

  • Lee, Kwan-Suk;Lim, Hyeon-Kyo;Chang, Seong-Rok;Rhie, Kwang-Won;Kim, Yu-Chang
    • Journal of the Ergonomics Society of Korea
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    • v.27 no.1
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    • pp.37-43
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    • 2008
  • A lot of models have been developed for prevention of human errors. Nevertheless most of them failed to attract attention of industry which has been looking for an integrative model that can show practical countermeasures as well as causal factors of human errors. This research aimed to develop a comprehensive model that can mainly be applied to industrial fields. Therefore, in the model, it was tried to explain sequences of an operator's information process that might cause human errors on one hand, and life cycle stages of facilities involved when human errors occur on the other hand. This model was validated by using a typical accident case. With the comprehensive model presented in this research, one could follow up the sequence of human errors caused by operators, and errors made at the design stage which might cause accidents could be tracked. As a consequence, it is expected that much attention would be paid to preventing human errors in industrial fields since safety personnel can easily find out cause of human errors throughout life cycle stages of man-machine facilities if utilizing the suggested model.

Multi-aperture Photometry Pipeline for DEEP-South Data

  • Chang, Seo-Won;Byun, Yong-Ik;Kim, Myung-Jin;Moon, Hong-Kyu;Yim, Hong-Suh;Shin, Min-Su;Kang, Young-Woon
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.56.2-56.2
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    • 2016
  • We present a multi-aperture photometry pipeline for DEEP-South (Deep Ecliptic Patrol of the Southern Sky) time-series data, written in C. The pipeline is designed to do robust high-precision photometry and calibration of non-crowded fields with a varying point-spread function, allowing for the wholesale search and characterization of both temporal and spatial variabilities. Our time-series photometry method consists of three parts: (i) extracting all point sources with several pixel/blind parameters, (ii) determining the optimized aperture for each source where we consider whether the measured flux within the aperture is contaminated by unwanted artifacts, and (iii) correcting position-dependent variations in the PSF shape across the mosaic CCD. In order to provide faster access to the resultant catalogs, we also utilize an efficient indexing technique using compressed bitmap indices (FastBit). Lastly, we focus on the development and application of catalog-based searches that aid the identification of high-probable single events from the indexed database. This catalog-based approach is still useful to identify new point-sources or moving objects in non-crowded fields. The performance of the pipeline is being tested on various sets of time-series data available in several archives: DEEP-South asteroid survey and HAT-South/MMT exoplanet survey data sets.

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