• Title/Summary/Keyword: PSV

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Characterization of Peanut stunt virus Isolated from Black Locust Tree (Robinia pseudo-acacia L.)

  • Bang, Ju-Hee;Choi, Jang-Kyung;Lee, Sang-Yong
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.125-130
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    • 2006
  • An isolate of Peanut stunt virus (PSV) isolated from black locust tree (Robinia pseudo-acacia L.) showing severe mosaic and malformation symptoms, was designated as PSV-Rp. PSV-Rp was characterized by the tests of host range, physical properties, RNA and coat protein composition and RT-PCR analysis. Nucleotide sequences of the cucumoviruses CP genes were also used for identification and differentiation of PSV-Rp. Six plant species were used in the host range test of PSV-Rp. PSV-Rp could be differentiated from each Cucumovirus strain used as a control by symptoms of the plants. The physical properties of PSV-Rp virus were TIP $65^{\circ}C$, DEP $10^{-3}$, and LIP $2{\sim}3$ days. In dsRNA analysis, PSV-Rp consisted of four dsRNAs, but satellite RNA was not detected. Analysis of the coat proteins by SDS-PAGE showed one major protein band of about 31 kDa. RT-PCR using a part of Cucumovirus RNA3 specific primer amplified ${\sim}950bp$ DNA fragments from the crude sap of virus-infected black locust leaves. RFLP analysis of the RT-PCR product could differential PSV-RP from CMV The nucleotide sequence identity between the PSV-Rp CP and the TAV-P CP genes and the PS-V-RP CP and CMV-Y CP genes were 61.6% and 40.5%, respectively. On the other hand, the nucleotide sequence identity of the PSV-Rp CP gene was $70.9%{\sim}73.4%$ in comparison with those of PSV subgroup I (PSV-ER and PSV-J) and 67.3% with that of PSV subgroup II(PSV-W). Especially, the nucleotide sequence identity of PSV-Rp CP gene and that of PSV-Mi that was proposed recently as the type member of a novel PSV subgroup III was 92.4%.

Some Properties of an Isolate of Peanut stunt virus Isolated from White Clover (Trifolium repens L.) (토끼풀에서 분리한 Peanut stunt virus의 성질)

  • Jung, Goo-Ho;Jeon, Yong-Woon;Choi, Jang-Kyung;Hong, Jin-Sung;Ryu, Ki-Hyun;Lee, Sang-Yong
    • Research in Plant Disease
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    • v.14 no.1
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    • pp.71-75
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    • 2008
  • An isolate of Peanut stunt virus (PSV), named as Tr-PSV, was isolated from white clover (Trifolium repens L) showing mosaic symptom. Tr-PSV systemically infected all plants tested in the Nicotiana spp. and induced local lesions on inoculated leaves of Chenopodium amaranticolor. However, Tr-PSV induced typical mosaic symptoms as ER-PSV on Vigna unguiculata 5 to 6 days after inoculation, while Fny-CMV used as a control virus of Cucumovirus produced local lesions on inoculated leaves. In dsRNA analysis, Tr-PSV consisted of four dsRNAs, but satellite RNA was not detected. The cDNA of coat protein gene of Tr-PSV was amplified by RT-PCR using a Cucumovirus-specific single pair primers that designed to amplify a DNA fragment of approximately 950 bp. By restriction mapping analysis using RFLP of the RT-PCR products and by serological properties of gel diffusion test, Tr-PSV belongs to a typical member of PSV subgroup I. This is the first report on the occurrence of PSV in white clover in Korea.

Persimmon Vinegar Ingestion before Endurance Exercise on Energy Substrates Utilization (지구성 운동전 감식초 섭취시 에너지기질의 이용)

  • Seo, Hyobin;Nam, Ju-Ock;Jeon, Byung-Duk;Kim, Pan-Gi;Ryu, Sungpil
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.626-634
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    • 2012
  • The purpose of this study was to investigate the potential of persimmon vinegar as a functional beverage by analyzing the effects of persimmon vinegar ingestion on the energy substrate during endurance exercise. The healthy male adolescents (n=8) drunk persimmon vinegar ingested trial (PSV) or purified water ingested trial as the control trial (CON) 1 h prior to the exercise with the 60% of maximal oxygen uptake ($\dot{V}O_{2max}$) for 1 h. The exercise intensity was increased to the 80% of $\dot{V}O_{2max}$ and remained until exhaustion. And, the physiological variables, blood components, and amounts of energy oxidation were analyzed. There was no significant difference between trials in physiological variables, and the heart rates after exhaustion were higher in PSV compared to CON. There was no significant difference between trials in blood glucose level, while the blood lactic acids decreased significantly in PSV 30 and 60 minutes after onset of exercise. The free fatty acids concentration increased significantly in PSV from 15 minutes to 60 minutes after onset of exercise. The carbohydrate oxidation decreased significantly in PSV from 45 minutes after exercise and, on the contrary, the fatty acids oxidation increased significantly for the same period. And, fatty acids oxidation was higher in PSV compared to CON even after exhaustion. The respiratory exchange ratio was lower significantly in PSV compared to CON from 30 minutes to 60 minutes after exercise, whereas lower in CON after total exhaustion. The exercise time to exhaustion was 41% longer in PSV compared to CON. These results showed that the persimmon vinegar increase the level of lipids metabolism and decrease sense of fatigue by inhibiting carbohydrate oxidation during moderate intensity exercise, suggesting the possibility of using of persimmon vinegar as exercise functional beverage when ingested 1 h prior to the endurance exercise performance.

A Comparison of Clinical Efficacy of Weaning Method Between the Mode of Intermittent Mandatory Ventilation and Intermittent Mandatory Ventilation Plus Pressure Support (기계적 호흡 치료로부터의 이탈방법으로서 Intermittent Mandatory Ventilation 단독 사용과 Pressure Support를 병용한 Intermittent Mandatory Ventilation의 비교)

  • Choi, Jeong-Eun;Koh, Youn-Suck;Cho, Won-Kyoung;Lim, Chae-Man;Kim, Woo-Sung;Park, Pyung-Hwan;Choi, Jong-Moo;Kim, Won-Dong
    • Tuberculosis and Respiratory Diseases
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    • v.41 no.4
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    • pp.372-378
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    • 1994
  • Background: Pressure support ventilation(PSV) is a new form of mechanical ventilatory support that assists spontaneous inspiraory effort of an intubated patient with a clinician-selected amount of positive airway pressure. Low level pressure support during inspiration can overcome the resistive component of inspiratory work imposed by an endotracheal tube. However the clinical efficacy of PSV as a weaning method has not been established yet. Object: The aim of study was to evaluate the efficacy of PSV when it is added to intermittent mandatory ventilation(IMV) in facilitating weaning precess compaired to IMV mode alone. Method: When the subject patients became clinically stable with their arterial blood gas analysis in acceptable range, they underwent weaning process either by IMV alone or by IMV plus PSV. The level of pressure support was held constant throught the weaning period. For the patients who required mechanical ventilation for less than 72 hr, 2h weaning trial was performed with IMV rate starting from 6/min. For the patients who required mechanical ventilation more than 72 hr, 7 hr weaning trial was performed with IMV rate starting from 8/min. For the patients who failed three consecutive trials of weaning, retrial of weaning was attempted over 3 days with IMV rate starting from 8/min. Clinical characteristics, APACHE II score and nutritional status were compared. For all patients, heart rate, mean blood pressure and respiratory rate were mornitored for 48 hrs after weaning trial started. Results: The total number of weaning trial was 37 in 23 patients(18 by IMV, 19 by IMV+PSV). Total ventilation time, APACHE II score and nutritional status were not statistically different between the two groups. The weaning success rate were not statistically different(38.3% by IMV, 42.1% by IMV+PSV) and the changes of mean blood pressure, heart rate, respiratory rate during first 48 hours were not different between the two groups. Conclusion: Low level PSV when added to IMV for weaning trial does not seem to improve the success rate of weaning from mechanical ventilation. PSV at 10cm $H_2O$ did not induce significant physiologic changes during weaning process.

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Geomagnetic Paleosecular Variation in the Korean Peninsula during the First Six Centuries (기원후 600년간 한반도 지구 자기장 고영년변화)

  • Park, Jong kyu;Park, Yong-Hee
    • The Journal of Engineering Geology
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    • v.32 no.4
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    • pp.611-625
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    • 2022
  • One of the applications of geomagnetic paleo-secular variation (PSV) is the age dating of archeological remains (i.e., the archeomagnetic dating technique). This application requires the local model of PSV that reflects non-dipole fields with regional differences. Until now, the tentative Korean paleosecular variation (t-KPSV) calculated based on JPSV (SW Japanese PSV) has been applied as a reference curve for individual archeomagnetic directions in Korea. However, it is less reliable due to regional differences in the non-dipole magnetic field. Here, we present PSV curves for AD 1 to 600, corresponding to the Korean Three Kingdoms (including the Proto Three Kingdoms) Period, using the results of archeomagnetic studies in the Korean Peninsula and published research data. Then we compare our PSV with the global geomagnetic prediction model and t-KPSV. A total of 49 reliable archeomagnetic directional data from 16 regions were compiled for our PSV. In detail, each data showed statistical consistency (N > 6, 𝛼95 < 7.8°, and k > 57.8) and had radiocarbon or archeological ages in the range of AD 1 to 600 years with less than ±200 years error range. The compiled PSV for the initial six centuries (KPSV0.6k) showed declination and inclination in the range of 341.7° to 20.1° and 43.5° to 60.3°, respectively. Compared to the t-KPSV, our curve revealed different variation patterns both in declination and inclination. On the other hand, KPSV0.6k and global geomagnetic prediction models (ARCH3K.1, CALS3K.4, and SED3K.1) revealed consistent variation trends during the first six centennials. In particular, the ARCH3K.1 showed the best fitting with our KPSV0.6k. These results indicate that contribution of the non-dipole field to Korea and Japan is quite different, despite their geographical proximity. Moreover, the compilation of archeomagnetic data from the Korea territory is essential to build a reliable PSV curve for an age dating tool. Lastly, we double-check the reliability of our KPSV0.6k by showing a good fitting of newly acquired age-controlled archeomagnetic data on our curve.

DESIGN AND IMPLEMENTATION OF A PROTOCOL VERIFICATION SYSTEM (프로토콜 검증시스템의 설계 및 구현)

  • Kim, Yong-Jin
    • ETRI Journal
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    • v.11 no.4
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    • pp.22-36
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    • 1989
  • In this paper, a design and implementation of an efficient protocol verification system named LOVE has been described. The LOVE has been developed specifically for LOTOS. It performs not only protocol syntax validation (PSV) but also protocol functional verification(PFV). The PSV is a test to check if a protocol is free from protocol syntax errors such as deadlocks and livelocks. The PFV confirms whether or not a protocol achieves its functional objectives. For the PSV, the reachability analysis is employed, and the observational equivalence test is used for the PFV. For protocol verification using the LOVE, a schematic protocol verification methodology has been outlined.

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The Effect of an Aggressive Cool-Down Following A Refueling Outage Accident in which a Pressurizer Safety valve is Stuck Open

  • Lim, Ho- Gon;Park, Jin-Hee;Jang, Seung-Cheol
    • Nuclear Engineering and Technology
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    • v.36 no.6
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    • pp.497-511
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    • 2004
  • A PSV (pressurizer safety valve) popping test carried out in the early phases of a refueling outage may trigger a test-induced LOCA(loss of coolant accident) if a PSV fails to fully close and is stuck in a partially open position. According to a KSNP (Korea standard nuclear power plant) low power and shutdown PSA (probabilistic safety assessment), the failure of a high pressure safety injection (HPSI) accompanied by the failure of a PSV to fully close was identified as a dominant accident sequence with a significant impact on low power and shutdown risks (LPSR). In this study, we aim to investigate and verify a new means for mitigating this type of accident using a thermal-hydraulic analysis. In particular, we explore the applicability of an aggressive cool-down combined with operator actions. The results of the various sensitivity studies performed there will help reduce LPSR and improve Refueling outage safety.

Anti-Biofilm Effect of Egg Yolk Phosvitin by Inhibition of Biomass Production and Adherence Activity against Streptococcus mutans

  • Kim, Hyeon Joong;Lee, Jae Hoon;Ahn, Dong Uk;Paik, Hyun-Dong
    • Food Science of Animal Resources
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    • v.40 no.6
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    • pp.1001-1013
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    • 2020
  • The formation of biofilms on the enamel surface of teeth by Streptococcus mutans is an important step in dental plaque formation, demineralization, and early caries because the biofilm is where other bacteria involved in dental caries attach, grow, and proliferate. The objectives of this study were to determine the effect of phosvitin (PSV) on the biofilm formation, exopolysaccharides (EPS) production, adherence activity of S. mutans, and the expression of genes related to the compounds essential for biofilm formation (quorum-sensing inducers and components of biofilm matrix) by S. mutans. PSV significantly reduced the biofilm-forming activity of S. mutans and increased the degradation of preformed biofilms by S. mutans. PSV inhibited the adherence activity of S. mutans by 31.9%-33.6%, and the production of EPS by 62%-65% depending upon the strains and the amount of PSV added. The expressions of genes regulating the production of EPS and the quorum-sensing-inducers (gtfA, gtfD, ftf, relA, vicR, brpA, and comDE) in all S. mutans strains were down-regulated by PSV, but gtfB was down-regulated only in S. mutans KCTC 5316. Therefore, the anti-biofilm-forming activity of PSV was accomplished through the inhibition of biofilm formation, adherence activity, and the production of quorum-sensing inducers and EPS by S. mutans.

Priority Analysis of Cause Factors of Safety Valve Failure Mode Using Analytical Hierarchy Process (AHP를 활용한 안전밸브(PSV) 고장모드의 Cause Factors 우선순위 분석)

  • Kim, Myung Chul;Lee, Mi Jeong;Lee, Dong Geon;Baek, Jong-Bae
    • Korean Chemical Engineering Research
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    • v.60 no.3
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    • pp.347-355
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    • 2022
  • The safety valve (PSV) is a safety device that automatically releases a spring when the pressure generated by various causes reaches the set pressure, and is restored to a normal state when the pressure falls below a certain level. Periodic inspection and monitoring of safety valves are essential so that they can operate normally in abnormal conditions such as pressure rise. However, as the current safety inspection is performed only at a set period, it is difficult to ensure the safety of normal operation. Therefore, evaluation items were developed by finding failure modes and causative factors of safety valves required for safety management. In addition, it is intended to provide decision-making information for securing safety by deriving the priority of items. To this end, a Delphi survey was conducted three times to derive evaluation factors that were judged to be important in relation to the Failure Mode Cause Factor (FMCFs) of the safety valve (PSV) targeting 15 experts. As a result, 6 failure modes of the safety valve and 22 evaluation factors of its sub-factors were selected. In order to analyze the priorities of the evaluation factors selected in this way, the hierarchical structure was schematized, and the hierarchical decision-making method (AHP) was applied to the priority calculation. As a result of the analysis, the failure mode priorities of FMCFs were 'Leakage' (0.226), 'Fail to open' (0.201), 'Fail to relieve req'd capacity' (0.152), 'Open above set pressure' (0.149), 'Spuriously' 'open' (0.146) and 'Stuck open' (0.127) were confirmed in the order. The lower priority of FMCFs is 'PSV component rupture' (0.109), 'Fail to PSV size calculation' (0.068), 'PSV Spring aging' (0.065), 'Erratic opening' (0.059), 'Damage caused by improper installation and handling' (0.058), 'Fail to spring' (0.053), etc. were checked in the order. It is expected that through efficient management of FMCFs that have been prioritized, it will be possible to identify vulnerabilities of safety valves and contribute to improving safety.