• Clinical and Experimental Reproductive Medicine
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• 제23권3호
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• pp.341-349
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• 1996

초기배의 성판정은 대상가축의 성을 선발하는 수단으로써 뿐만아니라 인간의 유전적 질병의 조기진단법으로서 매우 가치가 크다. 체외수정 소 초기배의 성을 결정하기 위해 PCR과 PRINS를 이용하였으며 성판정에 이용된 8 세포${\sim)$배반포기 초기배는 체외수정후 난관상피세포와의 공배양에 의해 생산되었다. 초기배 의 DNA는 $200{\mu}g/ml$ proteinase K가 함유된 PCR lysis buffer에 하나의 초기배를 부유한 후 $50^{\circ}C$에서 1시간동안 처리하여 준비하였다. 중기 염색체 spreads는 초기배를 nocodazole로 처리한 후 air-drying 방법을 이용하여 준비하였다. 가능한 false positive signals을 배제하기 위해 소특이 및 Y 염색체 특이 primers를 이용하여 PCR을 수행한 결과, 웅성 초기배에서는 두 개의 증폭산물 (소특이 및 Y 염색체 특이)이 합성된 반면 자성 초기배에서는 하나의 증폭산물만 합성되었다. 한편 중기염색체상의 Y 염색체를 동정하기 위해 FISH와 PRINS를 수행한 결과, FISH에서보다 PRINS에서 더 강한 Y 염색체 특이 형광 signals이 탐지되었다. 이러한 결과는 PCR에 의한 체외생산 소 초기배의 신속정확하고 효율적인 성판정이 가능함을 보여주었다. 또한 PRINS를 통해 PCR 에 이용된 Y 특이 probe의 신뢰성이 염색체 수준에서 확인되었다.

• 한국안전학회지
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• 제26권6호
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• pp.85-89
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• 2011

Component reliability data were analyzed by using PRinS(Plant Reliability data information System) based on the latest operating experiences of eight KSNPs(Korea Standard Nuclear Power plants), and these new data were applied to the KSNP PSA models. In addition, the existing PSA models were revised for reflecting as-built and as-operated plant conditions. As a result of newly performing PSA in this paper, CDF and LERF were estimated 26.1% and 18.2% lower than the existing values, respectively. It was identified that the risk measures decreased not because of revising the models but because of applying the new component reliability data. The result and the method of this paper could be used when generating plant specific data and performing the living PSA in the future.

• Journal of applied mathematics & informatics
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• 제7권2호
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• pp.539-552
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• 2000

In this paper, we established the general comparison principles for IVP of impulsive differential equations with time variables, which strictly extend and improve the precious comparison results obtained by V. Lakes. et.al . and S.K.Kaul([3]-[7]). Whit the general comparison results, we constructed the monotone iterative sequences of solution for IVP of such equations which converges the maximal and minimal and minimal solutions , respectively.

• Advances in Computational Design
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• 제7권2호
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• pp.139-151
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• 2022

The article is devoted to the actual problem of studying the possibilities of implementing personalization of the user interface in accordance with the personality psychotypes. The psychological aspect of user interface design tools is studied and the correspondence of their application to the manifestations of personality psychotypes is established. The results of the distribu-tion of attention of users of these categories on the course page of the educational platform are presented and the distribution of attention in accordance with the focus on educational material is analyzed. Individual features and personal preferences regarding the used design tools are described, namely the use of accent colors in interface design, the application of the prin-ciples of typographic hierarchy, and so on. In accordance with this, the prospects for implementing personalization of the user interface of the educational platform are described. The results of the study allow us to state the relevance of developing and applying personalization of the user interface of an educational platform to improve learning outcomes in accordance with the psychological impact of individual design tools, and taking into account certain features of user categories. The research is devoted to the study of user attention concentration using heatmaps, in particular based on eyetreking technology, we will investigate the distribution of user attention on the course page of an educational platform Ta redistribution of atten-tion in accordance with certain categories of personality psychotypes. The results of the study can be used to rearrange the LMS Moodle interface according to the user's psychotype to achieve the best concentration on the training material. The obtained data are the basis for developing effective user interfaces for personalizing educational platforms to improve the quality of the education.