• Journal of Life Science
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• v.16 no.3 s.76
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• pp.485-492
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• 2006

The PrrBA two-component system is one of the major regulatory systems that control expression of photosynthesis genes in response to changes in oxygen tension in the anoxygenic photosynthetic bacterium, Rhodobacter sphaeroides. The system consists of the PrrB histidine kinase and the PrrA response regulator. The N-terminal transmembrane domain of PrrB serves as a signal-sensing domain and comprises six transmembrane helices forming three periplasmic loops and two cytoplasmic loops. The $3^{rd}$ and $4^{th}$ transmembrane helices and the $2^{nd}$ periplasmic loop were suggested to play a crucial role in redox-sensory function. In this study we demonstrated that mutations of Asp-90, Gln-93, Leu-94, Leu-98, and Asn-106 in the $2^{nd}$ periplasmic loop and its neighboring region led to severe defects in PrrB sensory function, indicating that these amino acids might be related to the redox-sensing function of PrrB. The mutant forms (D90E, D90N, and D90A) of PrrB were heterologously overexpressed in Escherichia coli, purified by means of affinity chromatography and their autokinase activities were comparatively assessed. The D90N form of PrrB was shown to possess higher autokinase activity than the wild-type form of PrrB, whereas the D90E form of PrrB displayed lower autokinase activity than the wild-type form of PrrB. The D90A mutation led to the loss of PrrB autokinase activity.

• BMB Reports
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• v.52 no.2
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• pp.157-162
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• 2019

Our previous study found that two novel cancer-related genes, PRR11 and SKA2, constituted a classic gene pair that was regulated by p53 and NF-Y in lung cancer. However, their role and regulatory mechanism in breast cancer remain elusive. In this study, we found that the expression levels of PRR11 and SKA2 were upregulated and have a negative prognotic value in breast cancer. Loss-of-function experiments showed that RNAi-mediated knockdown of PRR11 and/or SKA2 inhibited proliferation, migration, and invasion of breast cancer cells. Mechanistic experiments revealed that knockdown of PRR11 and/or SKA2 caused dysregulation of several downstream genes, including CDK6, TPM3, and USP12, etc. Luciferase reporter assays demonstrated that wild type p53 significantly repressed the PRR11-SKA2 bidirectional promoter activity, but not NF-Y. Interestingly, NF-Y was only essential for and correlated with the expression of PRR11, but not SKA2. Consistently, adriamycin-induced (ADR) activation of endogenous p53 also caused significant repression of the PRR11 and SKA2 gene pair expression. Notably, breast cancer patients with lower expression levels of either PRR11 or SKA2, along with wild type p53, exhibited better disease-free survival compared to others with p53 mutations and/or higher expression levels of either PRR11 or SKA2. Collectively, our study indicates that the PRR11 and SKA2 transcription unit might be an oncogenic contributor and might serve as a novel diagnostic and therapeutic target in breast cancer.

• Proceedings of KOSOMES biannual meeting
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• 2003.05a
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• pp.135-139
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• 2003

This paper describes on the development of Passive-type Radar Reflector for Fisheries (PRR-F) based on the newly revised 2000 SOLAS regulations. The purpose of PRR-F is to provide it as the protection devices of a fishing net and a fishing field. The PRR-F is composed of corner cluster bundle of light galvanized iron, and it is inserted into floating styrofoam. Performance tests for the PRR-F are carried out in an anechoic chamber. The test results show that the reflected radar signal from PRR-F is large enough for the purpose.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5479-5486
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• 2012

In this paper, we have measured PRR, RSSI and LQI of ZigBee channels under Wi-Fi environment and have assessed channel characteristic and link quality. To confirm any relationship among RSSI, LQI values and PRR under Wi-Fi interference in overlapping and non-overlapping channels of Wi-Fi and ZigBee, the experiments were performed without Wi-Fi, with Wi-Fi and file download through Wi-Fi. Under Wi-Fi interference, We perfomed experiments to ensure channel characteristics and link quality by fixing Wi-Fi and ZigBee receiver and varying the distance between ZigBee receiver and transmitter. ZigBee transmitter sends packet of 256 bits every second to ZigBee receiver. PRR was measured from ZigBee with variance of distance between fixed Wi-Fi and ZigBee. RSSI, LQI, PRR were measured from ZigBee with fixed Wi-Fi, fixed ZigBee receiver and variance of distance of ZigBee transmitter. As a result, we confirmed decrease of PRR under Wi-Fi interference but RSSI, LQI values similar regardless of overlapped or non-overlapped channel and Wi-Fi interference. Therefore, PRR should be used for interference detection in ZigBee communication under Wi-Fi environment but RSSI and LQI are not appreciate.

• Proceedings of KOSOMES biannual meeting
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• 2003.05a
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• pp.125-134
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• 2003

This paper describes on the design of Passive-type Radar Reflector for small Ship (PRR-S) based on the newly revised 2000 SOLAS regulations. The design idea, adopted in the study, is to hold PRR-S in the proper ‘catch rain’ position to avoid fluctuations of Radar Cross Section (RCS) due to ship's heeling. The PRR-S consists of octahedral-type radar reflector with circular plates and three-axis gimbaled stabilizer with weight on the bottom of outer gimbal ring. Performance test for the PRR is carried out in an anechoic chamber. The test results show that the reflected radar signal from PRR-S is more uniformly distributed than the reference model (Davis Echomaster).

• Molecules and Cells
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• v.38 no.1
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• pp.81-88
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• 2015

Flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative short-day (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, Heading date 6 (Hd6) and Hd16 encode $CK2{\alpha}$ and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37;hereafter PRR37) and Ghd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and $CK2{\alpha}$ directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and $CK2{\alpha}$ interact with PRR37. We further use in vitro kinase assays to show that CKI and $CK2{\alpha}$ phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed.

• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.340-347
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• 2022

Advanced or metastatic breast cancer affects multiple organs and is a leading cause of cancer-related death. Cancer metastasis is associated with epithelial-mesenchymal metastasis (EMT). However, the specific signals that induce and regulate EMT in carcinoma cells remain unclear. PRR16/Largen is a cell size regulator that is independent of mTOR and Hippo signalling pathways. However, little is known about the role PRR16 plays in the EMT process. We found that the expression of PRR16 was increased in mesenchymal breast cancer cell lines. PRR16 overexpression induced EMT in MCF7 breast cancer cells and enhances migration and invasion. To determine how PRR16 induces EMT, the binding proteins for PRR16 were screened, revealing that PRR16 binds to Abl interactor 2 (ABI2). We then investigated whether ABI2 is involved in EMT. Gene silencing of ABI2 induces EMT, leading to enhanced migration and invasion. ABI2 is a gene that codes for a protein that interacts with ABL proto-oncogene 1 (ABL1) kinase. Therefore, we investigated whether the change in ABI2 expression affected the activation of ABL1 kinase. The knockdown of ABI2 and PRR16 overexpression increased the phosphorylation of Y412 in ABL1 kinase. Our results suggest that PRR16 may be involved in EMT by binding to ABI2 and interfering with its inhibition of ABL1 kinase. This indicates that ABL1 kinase inhibitors may be potential therapeutic agents for the treatment of PRR16-related breast cancer.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.8
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• pp.15-21
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• 1996

This paper describes data placement schemes that provide uniform and balanced to multiple disks load for retrievals of VBR (variable bit rate) video at varying retrieval speeds. To support maximum concurent users at arbitrary-speed playbacks in a disk-arry based system, the hot spot disks should be carefully avoided. In this paper, we extend the proposed scheme, prime round-robin(PRR), for VBR video. In addition, we have compared the performance of PRR and PRR (PRR extension).

• Molecules and Cells
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• v.26 no.1
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• pp.5-11
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• 2008

Stalled DNA replication forks activate specific DNA repair mechanism called post-replication repair (PRR) pathways that simply bypass DNA damage. The bypassing of DNA damage by PRR prevents prolonged stalling of DNA replication that could result in double strand breaks (DSBs). Proliferating cell nuclear antigen (PCNA) functions to initiate and choose different bypassing pathways of PRR. In yeast, DNA replication forks stalled by DNA damage induces monoubiquitination of PCNA at K164, which is catalyzed by Rad6/Rad18 complex. PCNA monoubiquitination triggers the replacement of replicative polymerase with special translesion synthesis (TLS) polymerases that are able to replicate past DNA lesions. The PCNA interaction motif and/or the ubiquitin binding motif in most TLS polymerases seem to be important for the regulation of TLS. The TLS pathway is usually error-prone because TLS polymerases have low fidelity and no proofreading activity. PCNA can also be further polyubiquitinated by Ubc13/ Mms2/Rad5 complex, which adds an ubiquitin chain onto monoubiquitinated K164 of PCNA. PCNA polyubiquitination directs a different PRR pathway known as error-free damage avoidance, which uses the newly synthesized sister chromatid as a template to bypass DNA damage presumably through template switching mechanism. Mammalian homologues of all of the yeast PRR proteins have been identified, thus PRR is well conserved throughout evolution. Mutations of some PRR genes are associated with a higher risk for cancers in mice and human patients, strongly supporting the importance of PRR as a tumor suppressor pathway.

• Journal of Navigation and Port Research
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• v.27 no.3
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• pp.267-272
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• 2003

This paper describes design method of Passive-type Radar Reflector (PRR) which is to provide the requirement of newly revised 2000 SOLAS regulations on the Radar Reflector. The main target of this work is to find the optimum shape of a radar target having large Radar Cross Section (RCS). Through the RCS analysis based on the theoretical approach, two kinds of PRR models, RRR-F model for use in fisheries and PRR-S model for use in small sized ship, are designed and discussed their RCS performance. RCS measurement tests for the various sized samples are carried out in an anechoic chamber. As evaluation results it was clearly shown that the conventional sphere-type shows optimum shape in case of PRR-S, while the cylinder-type which consists of large sized corner clusters or zig-zag flat plats gives best performance in case of PRR-F.