• Title/Summary/Keyword: PROPAGATION

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Effects of Auxin and Fog Treatments on the Green-Wood Cutting of the Mature Trees in Prunus yedoensis (왕벚나무 성숙목의 녹지삽목에서 Auxin 및 Fog 처리 효과)

  • Kim, Chang-Soo;Kim, Zin-Suh
    • Journal of Korean Society of Forest Science
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    • v.96 no.6
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    • pp.676-683
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    • 2007
  • In an attempt to develop an efficient method for the propagation of mature Prunus yedoensis (45 to 55 years old), green wood cuttings from normal branch and sprouts branch were treated with three different kinds of auxin (Rootone < 1-naphthylacetamide 0.4% >, IBA 100 ppm, and control and two different kinds of fog systems (0.9 L/min. and 0.54 L/min.). The Rootone treatment showed higher values in the percentage of rootings (PR) and the mean number of roots per cutting (NR) than the IBA treatment in the early stage. However, in the late stage, the values of PR and NR in the Rootone treatment become lower than those in the IBA 100 ppm treatment. On the other hand, root development ceased 62 days after taking cuttings for all of the treatments. The IBA 100 ppm treatment showed the best performance in root development (PR= 89.5%, NR = 6.5, LR=6.4 cm). The values of PR (76.5%) and NR (6.4) in the 0.9 L/min. of fog treatment was higher than those (PR = 71.7% and NR = 5.4) in the 0.54 L/min. of fog treatment. The cuttings from sprouts (PR: 74.8%, NR: 5.9, LR: 5.7 cm) showed slightly better performance in rooting rate that the cuttings from shoots (PR : 73.3%, NR: 5.9, LR: 5.4 cm). Statistically significant interactions were presented among most of the different combinations of three factors (auxin treatments, fog treatments, and types of cuttings). The PR showed the highest value of 98.0% in the combination of cuttings of shoots+IBA 100 ppm+0.54 L/min. fog treatments. In case of NR, the cuttings from normal branch showed a higher value than the cuttings from sprouts branch under the fog treatment of 0.9 L/min., while this tendency was reversed under the fog treatment of 0.54 L/min.. The perigon development of roots, which reflects the number and the direction of roots, was best in the IBA treatment (85.6%).

Lowered Substrate pH Reduced the Bicarbonate Injury during Vegetative Growth of 'Ssanta' Strawberry (혼합상토의 pH 저하가 영양생장 중인 '싼타' 딸기의 중탄산 피해 경감에 미치는 영향)

  • Lee, Hee Su;Cheung, Jong Do;Choi, Jong Myung
    • Journal of Bio-Environment Control
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    • v.26 no.2
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    • pp.115-122
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    • 2017
  • Objective of this research was to investigate the influence of lowered substrate pH on the reduction of bicarbonate injury in the vegetative growth of 'Ssanta' strawberry. The acid substrate was formulated by mixing sphagnum peat moss with pine bark (5:5, v/v) and the pH, EC, and CEC of the substrate were 4.07, $0.46dS{\cdot}m^{-1}$, and $91.3cmol^+{\cdot}kg^{-1}$, respectively. To adjust the pH of acid substrate, various amount of dolomitic lime [$CaMg(CO_3)_2$] were incorporated with the rate of 0 (untreated), 1, 2, 3, and $4g{\cdot}L^{-1}$. Then, mother plants were transplanted and grown with fertilizer solution containing $240mg{\cdot}L^{-1}$ of the $HCO_3{^-}$ and equal concentrations of essential nutrients to Hoagland solution. In growth of 'Ssanta' strawberry, fresh weight of mother plants were the highest in the treatment of $2g{\cdot}L^{-1}$ dolomitic lime such as 102.1 g followed by 94.7 g in $1g{\cdot}L^{-1}$, 91.2 g in $3g{\cdot}L^{-1}$, 75.7 g in $0g{\cdot}L^{-1}$ and 72.3 g in $4g{\cdot}L^{-1}$ treatments. The dry weight showed a similar tendency to fresh weight. At 140 days after transplanting, 5.8, 9.8, 11.8, 8.8, and 5.0 daughter plants were derived from each of the mother plants in the treatments of 0, 1, 2, 3, and $4g{\cdot}L^{-1}$ dolomitic lime, respectively. The highest occurrence of daughter plants were observed in the treatments $2g{\cdot}L^{-1}$ dolomitic lime. The substrate pH and bicarbonate concentration of 'Ssanta' strawberry seedlings in the 1 and $2g{\cdot}L^{-1}$ dolomitic lime treatments were maintained at a proper range such as 5.6 to 6.2. The micro-nutrient contents of above ground tissue in mother plants were the highest in $2g{\cdot}L^{-1}$ and the lowest in $4g{\cdot}L^{-1}$ dolomitic lime treatment. The above results indicate that incorporation rate of dolomitic lime in acid substrate with the pH of around 4 is $2g{\cdot}L^{-1}$ to raise the 'Ssanta' strawberry in propagation.

Effect of $CO_2$ Enrichment on the Differentiation of Multi-shoots and Saponin contents in Tissue culture of Korean ginseng (Panax ginseng C. A. Meyer) (인삼(人蔘) 조직배양(組織培養)에서 $CO_2$처리(處理)가 multi-shoot 분화(分化) 및 사포닌 함량(含量)에 미치는 영향(影響))

  • Chung, Chan-Moon;Bae, Kil-Kwan
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.4
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    • pp.296-302
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    • 1999
  • This experiment was conducted to study the effect of $CO_2$(0, 2, 500, 5, 000, 10, 000ppm) enrichment by enabling ventilation on micropropagation of multi-shoot and on the saponin contents in vitro in Korean ginseng (Panax ginseng C. A. Meyer). Embryo was cultured in Murashige and Skoog medium added 3mg/ l of Indolbutyric acid, Benzyladenin and Gibberellic acid $(GA_3)$, respectively. $CO_2$, enrichment had little effects on the number of adventitious buds and shoots originated from adventitious buds. The ratio of differentiated shoots to adventitious buds were about 50% in $CO_2$, enrichment treatment. The shoots originated from adventitious bud showed more rapid growth and had larger leaf area than the shoots originated from the leaf primordia did. The number of shoot primordia was the highest in 2, 500ppm of $CO_2$ enrichment treatment. On the contrary, 10,000ppm of $CO_2$, enrichment made smaller the number of shoot primordia and ratio of shoots to shoot primordia. The range of shoots differentiated was from shoot primordia were 15. 4 to 23. 9. The rate of dry weight of cultured shoots showed lowest (7. 5%) in control and highest (8. 59%) in 2, 500ppm of $CO_2$, enrichment. Rate of in vitro flower in control was 7.6% and that in 2500ppm of $CO_2$ was about twice (15.7-16.3%) as much as in control. Flower number per a embryo cultured was about 1.2-1.3. In the multi-shoots with callus enriched by 2, 500ppm of $CO_2$, the contents of crude saponin and ginsenosides in multi-shoots alone were higher than in multi-shoots with callus. The characteristics of ginsenosides in multi-shoots were especially the higher content of ginsenoside Rd, Re, and $Rg_1$.

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Studies on the Bare Rock-slope Conservation Measures (I) -Conservation and Revegetation by Parthenocissus spp.- (암벽면녹화공법개발(岩壁面綠化工法開發)에 관(關)한 연구(硏究)(I) - 담쟁이덩굴류(類)의 이용성개발(利用性開發) -)

  • Woo, Bo-Myeong
    • Journal of Korean Society of Forest Science
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    • v.37 no.1
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    • pp.1-16
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    • 1978
  • The study describes on the identification and morphological characteristics of each species, ecological characteristics and propagation techniques, and developing utilization measures of the Parthenocissus plants for environment conservation and revegetation of the babe rock-slopes. The following species and varieties are disscussed in this study; Parthenocissus tricuspidata (S. et Z.) Planch. var. veitchii Rehd. var. lowii Rehd. var. pupurea Hort. Parthenocissus quiquefolia (L.) Planch. var. engelmanni Rehd. var. saint-pauli Rehd. var. hirsuta Planch. Parthenocissus henryana Diels et Gilg. Parthenocissus thomsoni Planch. Parthenocissus heptaphylla Small. Parthenocissus inserta (Kern.) K. Fritsch. Parthenocissus laetevirens Rehd. Parthenocissus himalayana Planch. These are, in general, all vigorous self-clinging climbers that will quickly cover a wall and bare rock surfaces with a dense network of branch growths and beautiful green leaves which change to shades of scarlet and crimson before they fall in Autumn. Parthenocissus tricuspidata out of 8 species in the genus Parthenocissus is the most useful plant for the environment conservation including the bare rock-slope revegetation and for the production of food and shelter for wildlifes. This native of Korea clings by means of small rootlike holdfasts (adhesive discs) and holds (tendrils) to stone work or any other solid support, tenaciously.

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The Effects of Maternal HBs antigenemia on the Neonatal Health (산모(産母)의 B형 간염(肝炎) Virus 보유(保有)가 신생아(新生兒) 건강(健康)에 미치는 영향(影響))

  • Park, Jung-Han;Yoon, Sung-Do;Kim, Chang-Youn;Lee, Sung-Kwan
    • Journal of Preventive Medicine and Public Health
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    • v.17 no.1
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    • pp.47-55
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    • 1984
  • To study the risk factors associated with maternal HBsAg carrier and the effects of maternal HBs antigenemia on the neonatal health, sera of 729 pergnant women admitted to the Keimyung University Hospital for delivery during the period of February 1-May 30. 1982 were tested for HBsAg by RPHA method and for anti-HBs by PHA method. Among them 43 women (5.9%) had HBsAg and 246 women (33.7%) had anti-HBs giving an infection rate of 39.6%. The interview data for 43 HBsAg positive mothers and randomly selected 210 HBsAg negative mothers showed a statistically significant association between acupuncture history and HBsAg positive rate (p<0.005) which suggest that acupuncture might have contributed significantly to the propagation of viral hepatitis in Korea. The living standard of HBsAg positive mothers was generally lower than that of HBsAg negative mothers which supports the hypothesis that environmental factors are associated with viral hepatitis B infection. None of the 43 neonates born to HBsAg positive mothers had HBsAg in their cord blood. Three months after birth, 35 out of 43 infants were retested and only one infant became HBsAg positive. At six months of age, 32 out of 35 infants were retested and none of them were HBsAg positive except the same infant who was positive at three months. Among 20 control infants of HBsAg negative mothers, all of them were HBsAg negative at three and six months follow-up. These findings are not consistent with the supposition that perinatal infection is a main route of viral hepatitis B transmission in south-east Asia including Korea. HBsAg positive mothers had significantly higher rate of premature delivery (27.9%) than HBsAg negative mothers (11.7%) (p<0.05). Also, the low birthweight incidence rate was higher among HBsAg positive mothers (23.3%) than negative mothers (14.1%) but this was not statistically significant (P=0.16). The premature rupture of membrane was more frequent among HBsAg positive mothers (25.5%) than negative mothers (11.1%) (p<0.05). There were no significant differences in the stillbirth rate and incidence of congenital anomalies between HBsAg positive and negative groups. It was not clarified in this study due to small sample size whether higher incidence of premature delivery and premature rupture of membranes among HBsAg positive mothers was due to HBs antigenemia per so or their lower living standard than HBsAg negative mothers.

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Evaluation of Stress Thresholds in Crack Development and Corrected Fracture Toughness of KURT Granite under Dry and Saturated Conditions (포화유무에 따른 KURT 화강암의 균열손상 기준 및 수정 파괴인성 측정(Level II Method))

  • Kim, Jin-Seop;Hong, Chang-Ho;Kim, Geon-Young
    • Tunnel and Underground Space
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    • v.30 no.3
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    • pp.256-269
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    • 2020
  • The objective of this study is to evaluate the stress thresholds in crack development and the corrected fracture toughness of KURT granite under dry and saturated conditions. The stress thresholds were identified by calculation of inelastic volumetric strain from an uniaxial compression test. The corrected fracture toughness was estimated by using the Level II method (Chevron Bend specimen), suggested by ISRM (1988), in which non-linear behaviors of rock was taken into account. Average crack initiation stress(σci) and crack damage stress(σcd) under a dry condition were 91.1 MPa and 128.7 MPa. While, average crack initiation stress(σci) and crack damage stress(σcd) under a saturated condition were 58.2 MPa and 68.2 MPa. The crack initiation stress and crack damage stress of saturated ones decreased 36% and 47% respectively compared to those of dry specimens. A decrease in crack damage stress is relatively larger than that of crack initiation stress under a saturated condition. This indicates that the unstable crack growth can be more easily generated because of the saturation effect of water compared to the dry condition. The average corrected fracture toughness of KURT granite was 0.811 MPa·m0.5. While, the fracture toughness of saturated KURT granite(KCB) was 0.620 MPa·m0.5. The corrected fracture toughness of rock in saturated condition decreases by 23.5% compared to that in dry condition. It is found that the resistance to crack propagation decreases under the saturated geological condition.

Improvement of Arbuscular Mycorrhizal Fungi(AMF) Propagule at the Preplanting Field for Ginseng Cultivation (인삼 재배 예정지의 Arbuscular 균근균(AMF) 번식체 밀도 향상)

  • Sohn, Bo-Kyoon;Jin, Seo-Young;Kim, Hong-Lim;Cho, Ju-Sik;Lee, Do-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.3
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    • pp.170-176
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    • 2008
  • This study was carried out to improve density of arbuscular mycorrhizal fungi (AMF) propagule and physiochemical properties of soil by planting crops at the preplanning field for ginseng cultivation. Winter crops, such as barley and rye and summer crops, such as sudangrass and soybean were cultivated in combination to improve AMF propagation and soil aggregation at the fields. Yield of harvested crops by plating with winter or/and summer crops was $3,045kg\;10a^{-1}$ of the only rye cultivation, $2,757kg\;10a^{-1}$ of sudangrass cultivation in combination with rye growing (rye/sudangrass) and $1,628kg\;10a^{-1}$ of soybean cultivation in combination with barley growing (barley/soybean), respectively. Soil aggregation rate was improved by cultivation with barley (45.7%) and with rye/sudangrass (45.1%), respectively. The density of AMF spores in soil was increased slowly by cultivating with winter crops. In summer crops cultivation system, density of AMF spores at sudangrass cultivated field was $64.0spores\;g^{-1}$ dried soil and it was higher than that at soybean cultivated field. External hyphae length (EHL) was $1.5{\sim}2.0m\;g^{-1}$ air-dried soil at winter crops cultivated field. However, in summer crops cultivation systems, EHL was $2.6{\sim}2.9m\;g^{-1}$ airdried soil at sudangrass cultivated field and was $1.7{\sim}2.2m\;g^{-1}$ air-dried soil at soybean cultivated filed, showing these were higher than those in non-cultivated field (control). Glomalin content of soil cultivated with crops was higher than that of control soil. Especially, the highest glomalin content was shown to $1.7m\;g^{-1}$ air-dried soil in the barley/soybean cultivation systems. These results suggested that the most effective soil management to improve AMF propagule density and soil physical properties by planting crops system was cultivating sudangrass followed by barley at the preplanning fields for ginseng cultivation.

A Simulation-Based Investigation of an Advanced Traveler Information System with V2V in Urban Network (시뮬레이션기법을 통한 차량 간 통신을 이용한 첨단교통정보시스템의 효과 분석 (도시 도로망을 중심으로))

  • Kim, Hoe-Kyoung
    • Journal of Korean Society of Transportation
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    • v.29 no.5
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    • pp.121-138
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    • 2011
  • More affordable and available cutting-edge technologies (e.g., wireless vehicle communication) are regarded as a possible alternative to the fixed infrastructure-based traffic information system requiring the expensive infrastructure investments and mostly implemented in the uninterrupted freeway network with limited spatial system expansion. This paper develops an advanced decentralized traveler information System (ATIS) using vehicle-to-vehicle (V2V) communication system whose performance (drivers' travel time savings) are enhanced by three complementary functions (autonomous automatic incident detection algorithm, reliable sample size function, and driver behavior model) and evaluates it in the typical $6{\times}6$ urban grid network with non-recurrent traffic state (traffic incident) with the varying key parameters (traffic flow, communication radio range, and penetration ratio), employing the off-the-shelf microscopic simulation model (VISSIM) under the ideal vehicle communication environment. Simulation outputs indicate that as the three key parameters are increased more participating vehicles are involved for traffic data propagation in the less communication groups at the faster data dissemination speed. Also, participating vehicles saved their travel time by dynamically updating the up-to-date traffic states and searching for the new route. Focusing on the travel time difference of (instant) re-routing vehicles, lower traffic flow cases saved more time than higher traffic flow ones. This is because a relatively small number of vehicles in 300vph case re-route during the most system-efficient time period (the early time of the traffic incident) but more vehicles in 514vph case re-route during less system-efficient time period, even after the incident is resolved. Also, normally re-routings on the network-entering links saved more travel time than any other places inside the network except the case where the direct effect of traffic incident triggers vehicle re-routings during the effective incident time period and the location and direction of the incident link determines the spatial distribution of re-routing vehicles.

Effects of Temperature, Light and Plant Growth Regulators on the Seed Germination of Lavandula angustifolia Mill (잉글리쉬 라벤다의 종자발아(種子發芽)에 대한 온도(溫度), 광(光) 및 생육조절물질(生長調節物質)의 영향(影響))

  • Li, Xian Ri;Kang, Won-He;Yu, Chang-Yeon;Kim, Ii-Seop
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.258-264
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    • 1998
  • This study was conducted to establish mass propagation system from seeds of Lavandula angustiolia Mill. Only 4% of dry seeds were germinated when they were incubated in $4^{\circ}C$. Germination rate of stratified seeds for 9 weeks was 15% higher in light than darkness. Soaking with 1000mg/l of $GA_3$ was helpful to overcome the effect of darkness. The optimal temperature for germination was $25^{\circ}C$ for the seeds that were treated with$GA_3$, solution. For the improvement of germination rates, pretreatment of $GA_3$ at 500-2000mg/l showed about 75% of germination, and in the combination treatment of $GA_3$, and BA, germination rate increased by about 10% in the treatment of 1000mg/l $GA_3+10mg/l$ BA compared with the 1000mg/l $GA_3$ treatment. Cold stratification treatment was very effective for seed germination, and over 70% of seeds were germinated when they were incubated in $4^{\circ}C$ for 9-12 weeks. In addition, there was synergic effect on the seed germination subject to stratification and 1000mg/l $GA_3$, treatment for 6 months. In the mixture of vermiculite: peatmoss (1 : 1, v/v), emergence rate was 6.7% in control and 65% in 1000mg/l $GA_3$ treatment, respectively.

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A Morphological Study on the Granulosa Cell Apoptosis and Macrophages during Follicular Atresia in Pig Ovary (돼지난소에서 난포폐쇄시 과립층세포의 아포토시스와 대식세포에 관한 형태학적 연구)

  • Park, C.S.;Han, S.R.;Kim, S.I.;Cho, K.J.;Kim, W.S.;,
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.571-584
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    • 2004
  • It is known widely that granulosa cell apoptosis leads follicular atresia and macrophages exert their effects directly and/or indirectly from the initiation to the completion of follicular atresia by phagocytosis of apoptotic bodies and secretion of various cytokines. However, the site of initiation, propagation routes and the elimination methods of apoptotic bodies, and the time and methods of penetration of macrophages into the follicles are not known completely. Using pig(Yorkshire-breed) ovary, immunohistochemical studies with TUNEL for apoptotic bodies and pig macrophage monoclonal antibody 4E9 for macrophages, and light and transmission electron microscopic observations were performed. In the pig, follicular atresia began with the granulosa cell apoptosis, and the apoptosis of theca intema cells occured at the same time. The apoptosis of granulosa cells initiated randomly within the granulosa cell layer and propagated rapidly into the whole layer. Ultrastructura1ly, apoptotic granulosa cells showed characteristic pyknotic and deformed nucleus and intracytoplasmic vesicles. Apoptotic bodies were eliminated by intact granulosa cells and macrophages. Intact granulosa cells ingested apoptotic bodies transiently, soon after they fell into the apoptosis. Finally, apoptotic bodies and degenerated oocyte were phagocytosed by macrophages. Macrophages entered the ovarian follicle at the time of initiation of granulosa cell apoptosis, and migrated with the progression of apoptosis. By elimination of theca cells, macrophages contributed the completion of follicular atresia These results will provide valuable informations on the study of the interrelation between macrophage and ovarian follicular atresia.