• YAKHAK HOEJI
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• v.45 no.6
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• pp.708-714
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• 2001

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including preS region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, IY-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S protein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg mRNA as expected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.

• Journal of Biomedical Engineering Research
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• v.13 no.3
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• pp.209-216
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• 1992

A new experimental technique is proposed to localize the flow limiting segment(FLS) during forced expiration. The present technique is based on the pressure drip across FLS and a consequent change in airway resistance, which can provide an accurate and objective location of FLS. During forced expiratory maneuver artificially induced by a strong negative pressure (-100mmHg) applied at the trachea in an anesthetized open chest dog, airway resistance( R) was calculated from air flow and airway pres- sure signals at various airway locations and lung volumes, At the lung volumes above 10 % VC, FLS located in the trachea 6cm lower from the larynx. With the lung volume decreased below 8% VC, FLS jumped upstream to End-3rd generation of the airway. These results were similar with the previous reports from excised dog lungs, which demonstrated the validity of the present technique. Since the present technique provides a more objective measure of FLS location, it would be useful in future studies of expiratory flow limitation.

• Journal of Advanced Marine Engineering and Technology
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• v.23 no.5
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• pp.679-686
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• 1999

This study is to investigate the effect of a substantial drag reduction caused by the polymer(A611P) when the working fluids flow to the vertical direction in the vertical cylindrical equipment of closed flow system. The drag reduction is associated with the mechanical degrada-tion thermal degradation and heat transfer. By ignore the heat fluxs within the closed system the pressure drop due to the polymer concentration the flow velocity and flow time have been mea-sured. By taking into account the mechanical and thermal degradation in the closed system an experiment has been focused on the determination of the condition which could improve the pump capacity in the heat union electric power plant. Under the condition of non-boiling it has been found out that the change of heat flux has little influence on the drag reduction.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1999.05a
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• pp.556-559
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• 1999

In order to investigate the influence on dielectric characteristic due to physical structural change of specimens, we investigated the frequency and temperature dependency of tan $\delta$ for virgin specimen and mixtured specimens in the temperature range of 25[$^{\circ}C$] ~ 120[$^{\circ}C$], frequency range of 300(mv) ~ 1500[mV] An experimenatal specimen is selected as Low Liner Density Polyethylene (LLDPE) and Ethylene Vinyl Acetate(EVA) (thickness 200[$\mu$m]) produced by mixture ratio of 50:50, 60:40, 70:30 and 80:20. From the results of XRD it can be confirmed that the peak of LLDPE and EVA made by mixture ratio of 70:30 at 2$\theta$= 21.4$^{\circ}$is higher and the peak made by amorphous contribution at 2$\theta$= 19.5[$^{\circ}$] is almostly constant without no concern.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.1235-1237
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• 2003

For small capacity rectifier circuits as these for consumer electronics and appli capacitor input type rectifier circuits are gen used. Consequently. various harmonics gen within the power system become a serious pro Various studies of this effect have been pres previously. However. most of these employ swit devices, such as FETs and the like. The absen switching devices makes systems more toleran over -load, and brings low radio noise benefits propose a power factor correction scheme using resonant in commercial frequency without swit devices. In this method. It makes a sinusoidal by widening conduction period using the cu resonance in commercial frequency. Hence, harmonic characteristics can be significantly imp where the lower order harmonics. such as the and seventh orders are much reduced. The resu confirmed by the theoretical and experm implementations.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 1995.11a
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• pp.6-8
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• 1995

Among powder metallurgy products, complex shaped parts are difficult to be produced. Even i9f it shall be compacted until pet shape, the parts are easily apt to crack. thus it causes inferior productivity.It is present condition that net ahaped parts are obtained by adding after treatment. Clutch hub, pulley, representative partawifh upper 2 steps, tower 3 steps parts are corresponded to the above case. In recent years. muired parts are get to be thinner and thinner, and therefore extremely high compacting techniaues are required. Definition of most preferable com~actingp arts are I) Uniform density 2) Non crack and 3) Realization of net shape or near net shape productlon. For the above purpose, YOSHIZUKA baa developed n now oreas applying hydraulic servo control system.

• Food Science and Biotechnology
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• v.15 no.4
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• pp.523-528
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• 2006

The digestibility of heat- or high-pres sure-treated beef extracts was evaluated with an in vitro simulated gastric digestion model and each sample also underwent immune assay to detect its antigenicity with the sera of beef allergic patients. Heat treatment of the beef extracts considerably decreased their digestibility, whereas high-pressure treatment at 200 MPa improved their digestibility compared with the control, but the difference was not significant. The digestibility of the high pressure-treated beef extract was generally higher than that of the heat-treated samples. Depending on the degree of digestion, the degree of antigenicity of the main beef allergens decreased. On the basis of these results, we hypothesized that the allergenicity of beef could be eliminated if the allergenic proteins are sufficiently digested in the digestive organ, leading to the suggestion that the digestibility of allergenic proteins must be improved in food processing. In conclusion, high-pressure processing is a more acceptable food processing technique for beef considering its digestibility.

• Bulletin of the Korean Chemical Society
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• v.21 no.10
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• pp.957-958
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• 2000

The addition effect of $CH^3Br$ on the ignition of methane was investigated in the temperature range of 1537-1920 K behind reflected shock waves. The ignition delay times were measured by the sudden increase of pres-sure and OH emission in the $CH_4-O_2-Ar$ system containing small amount of $CH_3Br.$ The delay times of mix-tures with $CH_3Br$ were shorter than those without $CH_3Br.$ The promotion of ignition by $CH_3Br$ was caused by the relative fast decomposition rate in additive. To clarify the addition effect of $CH_3Br$ from the viewpoint of the reaction mechanism, computational analyses were performed in $CH_4-CH_3Br-O_2-Ar$ mixtures.

• Journal of Dental Rehabilitation and Applied Science
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• v.26 no.3
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• pp.311-322
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• 2010

The purpose of this study was to evaluate the bond strengths between IPS e.max Press and four different types of resin cements that often used for esthetic restoration. Disc shaped IPS e.max Press blocks(N=40, diameter: 12mm, thickness: 3mm) were fabricated according to the manufacture's instruction and cleaned with ultrasonic cleaner. They were embedded into an autopolymerizing acrylic resin. Fourty cylinder shaped resin block(Filtek Z350, diameter: 4.5mm, thickness: 3mm) were fabricated using a plastic tube. Each specimens were randomly divided into 4 experimental group and bonded each other using 4 different resin cements(Variolink II(light-cure), Variolink II(dual-cure), Calibra, Super-Bond C&B) according to the manufactures' recommendations. All the specimens were stored in normal saline at $37^{\circ}C$ for 24 hours before test. Universal testing machine at a crosshead speed of 1mm/min was used to evaluate the shear bond strength. The data were statistically analyzed using one-way ANOVA(P<.01). Multiple comparison was done by the Tukey HSD test. The shear bond strength of Super-Bond C&B to e.max was significantly lower than those of Calibra, Variolink II(light-cure), Variolink II(dual-cure) (P<.01). The shear bond strength of Calibra, Variolink II(light-cure), Variolink II(dual-cure) to e.max were not significantly different. The shear bond strengths of light-cure/dual-cure cement were higher than that of self-cure cement.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.109-118
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• 2016

Language intervention through cooking activity programs is designed to provide an efficient teaching method and improved educational environment in the field of teaching. This program addresses the effects of this program on the language development of three three-year-old infants (M;2, F;1) in the Center in Seoul. A cooking topic suitable for the age of this group was selected. The language Intervention was conducted for 50 minutes per week for a total of 25 times, and made use of vocabulary, verbs and nouns related to cooking which were evenly distributed. In this study, the Peabody Picture Vocabulary Test-Revised (PPVT-R), receptive language age (RLA) and expressive language age (ELA), and Preschool Receptive-Expressive Language Scale (PRES) were used to analyze the collected data. After the study, the cooking activity was accomplished with normal development outcomes appearing in the ability of vocabulary, receptive language, expressive language, and integrated language. There is now a solid evidence base supporting the efficacy of cooking activity in producing positive outcomes in the language development of language delayed infants. Consequently, cooking can induce their active participation and interest and extend their language abilities through various experiences.