• Parasites, Hosts and Diseases
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• v.39 no.1
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• pp.57-66
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• 2001

In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP 12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP 12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one you. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.

• Korean Journal of Applied Biomechanics
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• v.30 no.2
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• pp.155-163
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• 2020

Objective: This study to identify the mechanism of head impact that occurs during youth soccer game with regard to head injuries in sports. Method: Ten male subjects (age: 10.0±2.0 yrs.) were participated during 10 soccer practices spread out over a time period of 10 weeks. During each soccer game, the participants agreed and wore the X-Patch (wireless accelerometer, gyroscopes). The X-Patch records the head impact mechanics, such as peak linear acceleration (PLA), peak rotational acceleration (PRA), peak rotational velocity (PRV), Head Injury Criterion (HIC), and the location of impact. Results: A total of 501 impacts to the head were measured over the 10 soccer games, PLA 17.8±10.4 g, PRA 3168±2442 rad/s²; PRV 16.1±10.6 rad/s; HIC 11.7±34.2. The severity of impact was classified into 3 ranges; low 10~39 g (482 impacts); medium 40~69 g (17 impacts); and high >69 g (2 impacts). There are no significant differences in PLA and HIC (p=0.08, p=0.15), however PRA and PRV show the differences (p<.05) between each of the participants. For the analysis comparing between the soccer games, there are no significant differences in PLA, PRA, PRV and HIC (p=0.11, p=0.13, p=0.14, p=0.05). Conclusion: Our results indicated that there were significant differences between athletes, especially in terms of rotational acceleration, whereas there were significant differences in linear and rotational based variable between each of the soccer games. Although the vast majority of impacts were below 39 g there were 2 potentially dangerous impacts above 69 g. It is important that future research continuous to measure head impact mechanics during soccer to help understand head injury mechanisms to ensure the safety of athletes.

• Journal of the Korea Society of Computer and Information
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• v.17 no.1
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• pp.141-149
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• 2012

This paper proposes a resource management and allocation algorithm to guarantee QoS continuity of realtime multimedia services and to maximize the resource utilization in heterogeneous wireless networks simultaneously. This scheme reserves the appropriate resources to be used by real-time multimedia services and allocates those resources left unused due to decreased transmission rate of realtime services to non-realtime services. Those resources can be preempted by the need of realtime services later. Performances are analyzed by simulation.

• Nuclear Engineering and Technology
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• v.45 no.6
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• pp.767-790
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• 2013

MAAP4 is a computer code that can simulate the response of a light water reactor power plant during severe accident sequences, including actions taken as part of accident management. The code quantitatively predicts the evolution of a severe accident starting from full power conditions given a set of system faults and initiating events through events such as core melt, reactor vessel failure, and containment failure. Furthermore, models are included in the code to represent the actions that could mitigate the accident by in-vessel cooling, external cooling of the reactor pressure vessel, or cooling the debris in containment. A key element tied to using a code like MAAP4 is an uncertainty analysis. The purpose of this paper is to present a MAAP4 based analysis to examine the sensitivity of a key parameter, in this case hydrogen production, to a set of model parameters that are related to a Level 2 PRA analysis. The Level 2 analysis examines those sequences that result in core melting and subsequent reactor pressure vessel failure and its impact on the containment. This paper identifies individual contributors and MAAP4 model parameters that statistically influence hydrogen production. Hydrogen generation was chosen because of its direct relationship to oxidation. With greater oxidation, more heat is added to the core region and relocation (core slump) should occur faster. This, in theory, would lead to shorter failure times and subsequent "hotter" debris pool on the containment floor.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.868-871
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• 2004

Candida albicans has become the most important human pathogen in immunocompromised patients. One important feature of the pathogenicity in C. albicans is the morphological transition from yeast to hyphae. Previously, we reported that lysophosphatidylcholine (Lyso-PC) suppressed the hyphal transition through the MAP kinase pathway (Min et al., 2001). Therefore, it should be useful to examine the unknown genes involved in the MAP kinase pathway. As a way to identify target genes of Lyso-PC in hyphal suppression, this present study exploited two-dimensional electrophoresis. It was revealed that Lyso-PC suppressed expression of Phr1p and Pra1p, surface proteins involved in the morphogenesis.

• The Korean Journal of Archival Studies
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• no.6
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• pp.3-40
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• 2002

Appraisal is a basic archival function that analyzes values of records and determines the eventual disposal of records based upon their archival values. In Korea, this appraisal concept introduces in earnest through Public Records and Archives Act(PRA, 공공기관의 기록물관리에관한법률) with which Korean record management systems settle inflexibly. In theoreical and methodological area, therefore, it is necessary to analyze appraisal system in this Act with it in archival science. In PRA Act, appraisal system is founded on the Tables of Transaction for Records Scheduling(TRS, 기록물분류기준표) through which disposal activities of all records are definited in a concrete form. In this system, selection of archival materials which has been recognized as a important function of record center is carried out by record creators and archival institutions; Primary value between semi-currenty and non-currenty are reflected at the same time. In view of values, this appraisal system intends to separate reasonably consideration for continuing utility of achives from current use of records throughout agencies duration. Ultimately, appraisal based upon TRS makes up not separated management course but organic courses reflecting the Continuum of Care. Of course, this appraisal system makes up the deficiency partially. TRS regarded as 'mainboard' of current appraisal system will have to be enacted elaborately. And appraisal strategies of electronic records must set up in detail in PRA Act and TRS. Lastly, arrangement and description concepts immanent in TRS will have to supplement in archival institutions.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.3
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• pp.125-130
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• 2007

The aim of this work was to study the relationship between subjective symptoms and binocular function during near work in forty one(12-17yr) schoolchildren. CISS symptom survey, amplitude of accommodation, accommodative facility, neagative relative accommodation(NRA)/positive relative accommodation(PRA) near point of convergence(NPC), heterophoria, AC/A ratios were measured. The results of measurement were compared with normal expected value of each test and classified into normal and abnormal group. Accommodative insufficiency was more general than convergence insufficiency and esophoria was greater than exophoria. NRA/PRA showed very high and AC/A ratios were very low compare with normal expected value. The results of CISS was as follows; most frequent symptom was tired, mean score was $16.63{\pm}7.49$, mean number of symptoms were $10.07{\pm}3.04$, and mean number of serious symptoms were $1.98{\pm}2.13$. After classified into 4 groups according to score of symptoms, number of symptoms, and degree of symptoms and then analyze the correlation compare with binocular vision test results. NRA was most correlated with group comparison, accommodative facility was most correlated with score of symptoms, NRA was most correlated with number of symptoms, and near horizontal phorias were most correlated with serious symptoms. NRA showed most correlative relation with near symptoms.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.2
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• pp.48-53
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• 2013

Recently, the isolation rate of nontuberculous mycobacteria (NTM) in clinical laboratories and the incidence of NTM infections are on the increase in Korea, but there have been only a few studies that reveal the general aspect of NTM isolation or species distribution. Therefore, this study was performed to examine the species identification by PCR-restriction fragment length polymorphism analysis (PRA, PCR-RFLP), and the clinical significance of mycobacterial cultures. PRA was used during the novel region of the rpoB gene and was developed for rapid and precise identification of mycobacteria to the species level. From January 2012 to April 2012, we examined pre-identified nontuberculous mycobacteria (60 species in 3 hospital of Busan-Kyeongnam area). We confirmed 4 (6.6%) Mycobacterium tuberculosis (MTB) and 56 (93.4%) NTM from 60 pre-identified NTM species by multiplex PCR (MolecuTech $MTB-ID^R$ V3, YD Diagnostics, Korea) and PRA (Myco-ID, YD Diagnostics, Korea). The distribution of 56 NTM species were M. intracellulare type I 15 (26.7%), M. avium 14 (25%), M. abscessus 11 (19.5%), M. kansasii type I 3 (5.4%), M. pulveris 2 (3.6%), M. intracellulare type, M. chelonae, M. kansasii type V, M. gallinarum, M. wolinskyi. Respectively, 1 (1.8%) and 6 (10.7%) species were not identified.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.71-74
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• 2006

This study shows the historical background, and work flow of PRA (Probabilistic Risk Assessment) method which devised by NASA during the space development. It also illustrates the possibility of adoption of the method to evaluation of reliability KSLV project.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.04a
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• pp.3-10
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• 1999

This paper presents probabilistic risk assessment system model and methods for general construction projects and demonstrates the applicability of the approach to a specific subway construction project. The proposed system model entitled Integrated Risk Assessment System(IRAS) for construction projects is composed of four steps, which is newly reorganized and improved in order to be easily adjusted for a systematic PRA of construction projects. Based on the proposed model, and integrated prototype software is then developing for computer-aided PRA of construction projects under the environment of the graphic-user interface, which will be successfully applied to construction projects.