• Journal of Veterinary Science
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• v.22 no.4
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• pp.45.1-45.13
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• 2021

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 10⁶ cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

• Journal of Microbiology and Biotechnology
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• v.25 no.12
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• pp.2125-2134
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• 2015

IrrE is a highly conserved global regulator in the Deinococcus genus and contributes to survival from high doses of UV radiation, ionizing radiation, and desiccation. Drad-IrrE and Dgob-IrrE from Deinococcus radiodurans and Deinococcus gobiensis I-0 each share 66% sequence identity. However, Dgob-IrrE showed a stronger protection phenotype against UV radiation than Drad-IrrE in the D. radiodurans irrE-deletion mutant (ΔirrE), which may be due to amino acid residues differences around the DNA-binding HTH domain. Site-directed mutagenesis was used to generate a Drad-IrrE A184S single mutant, which has been characterized and compared with the ΔirrE mutant complemented strain with Drad-irrE, designated ΔirrE-E. The effects of the A184S mutation following UV radiation and mitomycin C (MMC) shock were determined. The A184S mutant displayed significantly increased resistance to UV radiation and MMC shock. The corresponding A184 site in Dgob-IrrE was inversely mutated, generating the S131A mutant, which exhibited a loss of resistance against UV radiation, MMC shock, and desiccation. qPCR analysis revealed that critical genes in the DNA repair system, such as recA, pprA, uvrA, and ddrB, were remarkably induced after UV radiation and MMC shock in the ΔirrE-IE and A184S mutants. These data suggested that A184S improves the ability against UV radiation and MMC shock, providing new insights into the modification of IrrE. We speculated that the serine residue may determine the efficiency of DNA binding, leading to the increased expression of IrrE-dependent genes important for protection against DNA damage.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2011.11a
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• pp.154-155
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• 2011

2010년 천리안위성의 성공적인 발사에 따라 인공위성의 활용에 대한 기대가 커지고 있다. 천리안 해양관측위성(GOCI)이외에 아리랑 2호가 현재 운용중인 우리나라 위성들이다. 가까운 시기에 아리랑 5호(2011년 말), 아리랑 3호(2012년), 아리랑 3A호(2013년)가 발사될 예정이다. 즉, 해양적용을 위한 위성환경은 이제부터 준비되고 있다고 볼 수 있다. 대외적으로 보면, 인공위성 자원은 아주 많다. 문제는 이와 같은 자원을 어떻게 활용할 것인가 인데 이의 활용 기술 개발적 측면에서는 많이 소홀한 것이 사실이다. 전세계적으로 이 시스템 개발을 위한 치열한 경쟁이 진행 중에 있다. 이미 소말리아 주변 감시체계는 많은 부분을 위성에 의지하고 있다. 우리나라에서 최초로 위성활용 가능성을 보여준 사건이 허베이스피리트호 원유유출 사고이다. 이 사고는 2007년 12월7일 아침 7시6분경 서해안 만리포 북서쪽 10km 해상에서 크레인을 적재한 1만1800t급 바지선이 정박 중인 홍콩 선적 유조선 허베이 스피리트호(14만6000t급)와 부딪치면서 발생했다. 이와 같은 기름 유출 사고의 경우, 유출 범위를 정확하게 이해하는 것이 중요하다. 거의 준비된 상태가 아님에도 불구하고 12월 8일 아침 최초로 유출된 기름을 모습을 보여주는 위성이미지(광학위성)가 얻어졌다. 하지만 이와 같은 자료가 관련 전문가가 이용할 수 있기까지 많은 시간이 소용되었고, 이 정보를 전달할 수 있는 방법도 없었다. 사실 단순한 이미지가 아니라 지리정보체계를 가진 오염정보를 제공할 방법도 준비도 되어 있지 못한 상황이었다. 본 발표를 통하여, 허베이스피리트호 사고뿐만 아니라, 2011년 6월부터 수개월간 지속된 발해만 오염사고 적용 등 다양한 사례 소개를 하고, 이를 기반으로 해양경찰청에서 업무활용을 위한 방안을 제시한다. 먼저, 해경청의 주요 임무인, 경비, 수색구조, 오염대응 분야별로 현황 분석을 수행하였다. 또한 국외사례에 대한 조사를 한 후, 최종 인공위성 원격탐사기술의 해경청 도입방안에 대한 설계를 실시하였다. 국제적으로 인공위성을 이용한 해양 경비, 수색구조, 오염 모니터링기술 개발이 이루어지고 있으며, 유럽 국가는 시범도입을 진행 중에 있다. 유럽해사안전국(EMSA)은 해양경비 및 수색구조를 위한 선박통항 및 보고 서비스와 오염대비대응(Pollution Preparedness and Response, PPR) 위성 서비스를 회원국에 제공하고 있다. 해양경찰청 임무 수행뿐만 아니라, 해양영토 관리적 측면에서 첨단 위성장비 활용, 선진국형 해상경비 패러다임의 전환 필요성이 크다고 할 수 있다.

• Journal of the Microelectronics and Packaging Society
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• v.18 no.4
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• pp.49-53
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• 2011

High-speed Cu filling into a through-silicon-via (TSV) and simplification of bumping process by electroplating for three dimensional stacking of Si dice were investigated. The TSV was prepared on a Si wafer by deep reactive ion etching, and $SiO_2$, Ti and Au layers were coated as functional layers on the via wall. In order to increase the filling rate of Cu into the via, a periodic-pulse-reverse wave current was applied to the Si chip during electroplating. In the bumping process, Sn-3.5Ag bumping was performed on the Cu plugs without lithography process. After electroplating, the cross sections of the vias and appearance of the bumps were observed by using a field emission scanning electron microscope. As a result, voids in the Cu-plugs were produced by via blocking around via opening and at the middle of the via when the vias were plated for 60 min at -9.66 $mA/cm^2$ and -7.71 $mA/cm^2$, respectively. The Cu plug with a void or a defect led to the production of imperfect Sn-Ag bump which was formed on the Cu-plug.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.477-484
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• 2002

Ninety pre-puberal (6-7 months) female and 15 pre-puberal male Black Bengal goats were collected on the basis of their phenotypic characteristics from different parts of Bangladesh. Goats were reared under semi-intensive management, in permanent house. The animals were vaccinated against Peste Des Petits Ruminants (PPR), drenched with anthelmentics and deeped in 0.5% Melathion solution. They were allowed to graze 6-7 h along with supplemental concentrate and green forages. Concentrates were supplied either 200-300 g/d (low level feeding) or quantity that supply NRC (1981) recommended nutrient (high level of feeding). Different physiological, productive and reproductive characteristics of the breed were recorded. At noon (temperature=$95^{\circ}F$ and light intensity=60480 LUX) rectal temperature and respiration rate of adult male and female increased from 100.8 to $104.8^{\circ}F$ and 35 to 115 breath/min, indicated a heat stress situation. Young female attain puberty at an average age and weight of 7.2$\pm$0.18 months and 8.89$\pm$0.33 kg respectively. Mean age and weight at 1st kidding were 13.5$\pm$0.49 months and 15.3$\pm$0.44 kg respectively. It required 1.24-1.68 services per conception with an average gestation length of 146 days. At low level of feeding the postpartum estrus interval was 37$\pm$2.6 days, which reduced (p<0.05) with high feeding level to 21$\pm$6.9 days. Kidding interval also reduced (p<0.05) from 192 d at low feeding level to 177 d at high feeding level. On an average there were two kiddings/doe/year. Average litter sizes in the 1st, 2nd, 3rd and 4th parity were 1.29, 1.71, 1.87 and 2.17 respectively. Birth weights of male and female kids were 1.24 and 1.20 kg respectively, which increased (p<0.05) with better feeding. Although kid mortality was affected (p<0.05) by dam's weight at kidding, birth weight of kid, milk yield of dam, parity of kidding, season of birth, but pre-netal dam's nutrition found to be the most important factor. Kid mortality reduced from 35% at low level of feeding to 6.5% at high level of feeding of dam during gestation. Apparently, this was due to high (p<0.05) average daily milk yield (334 vs. 556 g/d) and heavier and stronger kid at birth at high feeding level.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.4
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• pp.340-347
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• 2001

Seasonal variation of the elemental and biochemical composition of the suspended particulate matter (SPM) was investigated in terms of quantity and quality of diets for suspension feeders from July 1999 to August 2000 in two coastal bay systems of Gosung and Kangjin Bays in Korea. No clear patterns in the seasonal variations of SPM concentration were found in these two bay systems. The results indicated that the seasonal variation of SPM could not be considered the variation of food available to suspension-feeders. The simultaneous peaks in chlorophyll a and biochemical components in summer indicated that the quantity of the particulate organic matter primarily depended on phytoplankton productivity. However, no correlation between chlorophyll a and biochemical components [particulate protein (PPr), carbohydrate (PCHO) and lipid] were found, indicating that other processes might also contribute to the particulate organic matter in the period when the phytoplankton biomass was low. High C: Chl a and C:N, and carbohydrate peaks during the autumn to spring period suggested that resuspension of surface sediments was a probable process to supply the particulate organic matter. The food material, represented by summing up the total quantity of three biochemical components, was highest in spring with minor peaks during the period from autumn to the next spring, The food index, calculated as the ratio of food material to total SPM, did not generally exceed $6\%$ with short peaks during the year. Therefore, nutritional quality of SPM in the bays are relatively poor than in other more productive coastal waters in the world. Our results confirm that the measurement of a single chemical variable cannot describe fully the nutritive value of the seston available to suspension-feeders as previously proposed, and the biochemical composition of SPM can provide effective information on its origin and nutritive Quality.