• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1061-1065
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• 2001

The antibacterial effects of water extracts of Scutellariate Radix (a dried root of Scutellaria baicalensis GEORGI) and its major flavonoid components, Baicalin and Baicalein, on Salmonella typhimurium, a representative enteric pathogen, were studied. Through a Kriby-Bauer disc analysis, the growth-inhibition activity of Scutellariae Radix against. S. typhimurium was found to be compatible with commercial antibiotics, such as ampicillin, chloramphenicol, and streptomycin. In contrast, the growth of a nonpathogenic E. coli strain was unaffercted by Scutellariae Radix. To examine the effect of polyphosphate kinase (ppk), a putative virulence factor, on the antibacterial activity of Scutellariae Radix, the growth profile of a ppk mutant of S. typhimurium was investigated in a tryptic soy broth containing different concentrations of water extracts of Scutellariae Radix. The ppk mutant was able to grow in 6 mg/ml of water extracts of Scutellariae Radix, whereas in 6 mg/ml of water extracts of Scutellariae Radix, whereas the wild-type could not, implying that the inactivation of ppk made S. typhimurium more resistant to the antibacterial activity of Scutellariae Radix. No enhanced resistance was observed in a ppk mutant of S. typhimurium complemented with a ppk expression vector. The attenuation of the virulence by ppk inactivation was also observed in a virulence assay using BLAB/c mice. Neither Baicalin nor Baicalein exhibited any growth-inhibition activity against S. typhimurium. The water extracts of Scutellariae Radix stimulated the transcription of ppk, especially in the early growth-stage of S. typhimurium.

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권4호
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• pp.355-362
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• 2017

무인항공기는 최근 공간정보 분야 수요 증가에 따라 측량, 지도제작, 공간분석 등 다양한 분야에 활용되고 있으며, 신속한 데이터 취득이 가능하고, 경제성이 뛰어나기 때문에 많은 주목을 받고 있다. 본 연구에서는 무인항공사진측량을 도시방재정보 구축 분야에 적용하고자 하였다. 연구대상지에 대한 PPK(Post Processed Kinematic) GNSS 기반의 무인항공영상 취득 및 데이터 처리를 통해 수치표고모델과 정사영상을 생성하고, 결과물을 이용한 공간분석을 수행하였다. 또한 PPK GNSS 방법을 적용한 무인항공사진측량의 업무흐름을 기존 방법과 비교하였다. 연구를 통해 대상지역에 대한 도시방재정보를 효과적으로 구축할 수 있었으며, 기존 무인항공사진측량과 비교를 통해 PPK GNSS 방법의 효율성을 제시할 수 있었다. 향후 도시방재정보 구축 분야의 무인항공사진측량 활용에 있어 PPK GNSS 기법을 활용한다면 신속한 데이터 취득 및 처리로 관련 업무의 효율성을 크게 개선시킬 수 있을 것으로 기대된다.

• KAPE누리
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• 통권172호
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• pp.34-37
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• 2010

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.139-145
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• 2003

The gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and $39\%$ to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with $36\%$ yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and $70^{\circ}C$, respectively. A divalent cation was required for the enzyme activity, with $Mg^2+$ being the most effective.

• 한국지형학회지
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• 제28권4호
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• pp.41-52
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• 2021

With the popularization of drones and the ease of use of the Global Navigation Satellite System (GNSS), drone photogrammetry for terrain information has been widely used. Drone photogrammetry enables the realization of high-accuracy three-dimensional topography for the entire area with less effort and time compared to the past direct survey using GNSS or total station. From 3-D topographic data, various topographical analysis is possible. To improve the accuracy of drone photogrammetry, direct GCP surveying in the field is essential, and the numbers and reasonable positioning of GCPs are very important. In the case of beaches or tidal flats on the west coast of Korea, the numbers and location of GCPs are important factors in efficient drone photogrammetry because of the size of the area, difficulties of movement, and the risk from tides. If the RTK (Real-time kinematic) or PPK (Post-processed kinematic) method is used, the increased accuracy of the drone's location enables high-accuracy photogrammetry with a small number of GCPs. This study presents an efficient drone photogrammetry method in terms of time and economy by comparing and analyzing the results of drone photogrammetry using Non-PPK with low-cost PPK-Kit, based on the tests of various numbers and locations of GCPs in the university field including various slopes and structures like coastal terrain.

• 생명과학회지
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• 제10권4호
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• pp.397-402
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• 2000

본 연구는 인산 축적능이 뛰어난 균주를 분자 육종하여 생물학적 폐수처리 및 토양의 인산 집적을 해결시키는 산업적 유용한 재료로 이용하기 위한 기초연구를 목표로 하고 있다. Polyphosphate kinase의 ATP의 phosphate를 단리하여 한분자씩 결합시키는 형태로 polyphosphate의 합성반응을 촉매한다. 인산 축적에 관한 대사과정의 분자적 이해를 위하여 Serratia marcescens균주로부터 Southern hybridization방법으로 ppk를 암호하는 유전자를 찾아내어 새조합시킨 pDH3를 구축하였다. pDH3으로부터 ppk를 암호하는 유전자 영역의 4.0 kb 단편을 가진 subclone을 작성하였다.Serratia marcescens의 polyphosphate kinase의 활성은 catabolite repression에 의한 조절을 받았다. 발현멕타에 삽입시킨 재조합 플라스미드를 대장균에 도입시킨 결과, polyphosphate kinase의 효소활성이 크게 증가됨을 확인 하였다. 또한 대량 발현시킨 결과를 SDS-PAGE를 통하여 75 KDa의 발현산물을 확인할 수 있었다.

• Molecules and Cells
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• 제39권7호
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• pp.573-579
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• 2016

During copulation, male Drosophila transfers Sex Peptide (SP) to females where it acts on internal sensory neurons expressing pickpocket (ppk). These neurons induce a post-mating response (PMR) that includes elevated egg-laying and refractoriness to re-mating. Exactly how ppk neurons regulate the different aspects of the PMR, however, remains unclear. Here, we identify a small subset of the ppk neurons which requires expression of a pre-mRNA splicing factor CG3542 for egg-laying, but not refractoriness to mating. We identify two CG3542-ppk expressing neurons that innervate the upper oviduct and appear to be responsible for normal egg-laying. Our results suggest specific subsets of the ppk neurons are responsible for each PMR component.

• The Journal of Asian Finance, Economics and Business
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• 제7권10호
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• pp.491-499
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• 2020

The implementation of PPK-BLU in Indonesia was not followed by a comprehensive change in aspects of organization, human resources, and finance. Based on this background, this study aims to provide a comprehensive evaluation of the BTIP after the implementation of the PPK-BLU policy in the implementation of telecommunications services and information to the public in Indonesia. This research used integration of the McKinsey 7S method, Analytical Hierarchy Process, and Likert scale. The integration of these methods can provide a detailed quantitative analysis. Based on the overall evaluation results of carrying out the PPK BLU, BTIP has a performance value of 81.195%, which puts it within the Good category. The McKinsey 7S elements used as a whole have the following values: one aspect is categorized as Very Good, namely, the strategy aspect with a value of 91.025%; two aspects are categorized as Good (structural aspects with a value of 86.857%, and skill aspects with a value of 81.432%); three aspects are categorized as Adequate (style aspect with a value of 76.441%, staff aspect with a value of 78.605%, and share value aspect with a value of 76.331%); one aspect is categorized as Bad, that is the system aspect with a value of 67.503%.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1527-1535
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• 2009

Polyphosphate (polyP) plays diverse physiological functions in prokaryotes and eukaryotes, but most of their detailed mechanisms are still obscure. Here, we show that deletion of polyphosphate kinase (PPK), the principal enzyme responsible for synthesis of polyP, resulted in augmented expression of cAMP receptor protein (CRP) and rpoS and lowered $H_2O_2$ sensitivity in Salmonella Typhimurium ATCC14028. The binding of cAMP-CRP complex to rpoS promoter and further stimulation of its transcription were proved through electrophoretic mobility shift assay, lacZ fusion, and exogenous cAMP addition, respectively. The rpoS expression increased in cpdA (cAMP phosphodiesterase coding gene) mutant, further suggesting that cAMP-CRP upregulated rpoS expression. These results demonstrate that PPK affects oxidative stress response by modulating crp and rpoS expression in S. Typhimurium.

• 프린팅코리아
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• 제10권5호
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• pp.84-85
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• 2011