• 제목/요약/키워드: POx

검색결과 113건 처리시간 0.028초

Effect of Phytolacca americana Extracts on the Activities of AsPOX and GuPOX during Germination Process of Cassia mimosoides var. nomame

  • Lee, Ho-Joon;Kim, Yong-Ok
    • The Korean Journal of Ecology
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    • 제23권2호
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    • pp.187-192
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    • 2000
  • After Cassia mimosoides var. nomame was treated with Phytolacca americana extracts during the germination process, its effect on the activities of AsPOX and GuPOX were examined. As the concentration of P. americana extract increased. the activity of AsPOX decreased while that of GuPOX increased. During the germination process, the activity of AsPOX was lower than the control, while the activity of GuPOX was over 2 times higher than the control. Activity of total peroxidase by IEF was extremely high at pI 6.4 and pI 6.6 when treatment was made with a 30% concentration of P. americana extract. The treatment with phenolic compounds, caffeic acid and benzoic acid did not show much difference from the control although a slight increase was observed at pI 6.6. Activity of GuPOX in C. mimosoides was over 4 times higher in roots than in shoots. Namely, because GuPOX activity of C. mimosoides was increased by extracts of P. americana, defense enzyme, GuPOX. was generated against external stress, and we could certified the activity increase at pI 6.4, especially in root.

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국내 중간기착지에서 철새의 조류 폭스 감염 조사 (The survey on avian pox infections in migratory birds at a stopover site, South Korea)

  • 김희종;채희영;박성준;성환우;김종택
    • 한국동물위생학회지
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    • 제40권2호
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    • pp.149-153
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    • 2017
  • The present study was carried out to evaluate the effect of avian pox on wild bird population by investigating the avian pox infection in migratory birds of a stopover site. 3,565 birds in 116 species were examined for avian pox in migratory birds at the Heuksando island in South Korea during the spring and fall of 2011. 20 birds in 12 species were found pox-like lesions and 5 birds were diagnosed by avian pox using PCR: Pale Thrush (Turdus pallidus), Yellow-breasted Bunting (Emberiza aureola), Yellow-throated Bunting (Emberiza elegans), Rustic Bunting (Emberiza rustica), Black-faced Bunting (Emberiza spodocephala). To our knowledge, this is the first report of avian pox detected in these 5 species of the world.

계두예방약용(鷄痘豫防藥用) 계두독(鷄痘毒)과 구두독주(鳩痘毒株)의 비교연구(比較硏究) (Comparative Studies of Fowl and Pigeon pox Virus Strains for the Preparation of Fowl pox Vaccine)

  • 전윤성;김순재;정영석
    • 대한수의학회지
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    • 제5권1호
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    • pp.37-41
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    • 1965
  • For the preparation of an effective fowl pox vaccine, comparative studies of a number of fowl and pigeon pox virus strains were accomplished, and the following conclusions were made. 1. Anyang-Nakano strain which was nation widely used as a seed virus of fowl pox vaccine was proven its inadequacy. 2. A liquid vaccine prepared with Minnesota strain of pigeon pox virus showed its stability for 6 months and on side reaction.

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Sequential Induction of Ethylene, Lipoxygenase, and Ascorbate Peroxidase in Senescing Soybean Callus

  • Ha, Mi-Young;Kim, Do-Kyun;Kim, Soon-Young;Kang, Bin-G.;Oh, Seung-Eun
    • BMB Reports
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    • 제29권6호
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    • pp.564-568
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    • 1996
  • Bursts of ethylene production occurred in twice at an early exponential (EEP) and prestationary (PSP) phases, respectively, during growth of callus tissue isolated from the root of soybean seedlings. The second burst of ethylene production at PSP was smaller in magnitude than the earlier one at EEP, but was followed by increases in both guaiacol peroxidase (GuPOX) and ascorbate peroxidase (AsPOX). The increase in AsPOX activity was also preceded by an increase in lipoxygenase (LOX) activity. Treatment of the tissue with the ethylene antagonist 2,5-norbonadiene (NBD) resulted in substantial reduction in LOX and AsPOX activities during this period. GuPOX activity was reduced only slightly, if any, by NBD. Role of ethylene in the sequential induction of LOX and AsPOX in senescing callus tissue is discussed.

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Candida maltosa에서 분리된 n-Alkane 유도성 유전자(ALI1, POX18Cm)의 n-Alkane 대사에 있어서의 기능 (The Function of Two n-Alkane Inducible Genes (ALIl, POX18Cm) for n-Alkane Assimilating Candida maltosa)

  • 황철원;양창술
    • 한국미생물·생명공학회지
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    • 제21권2호
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    • pp.181-186
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    • 1993
  • The functions of n-alkane inducible genes, ALI1 and POX18Cm isolated from Canida maltosa were investigated, using it's distruptants. As a result, it is suggested that ALI1 is essential for n-alkane assimilation in C. mltosa and it regulates genes related to assimilation of n-alkane (ALI1, P450alk POX18Cm) at transcriptional level. Nuclear localization experiments indicated that ALI1 was located and functioned in the nucleus. POX18Cm is considered as a peroxisomal nonspecific lipid transfer protein gene related to n-alkane assimilation in C. maltosa also regulated by ALI1. But it had no significant effect on n-alkane assimilation in C. maltosa.

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Comparison of Promoters Suitable for Regulated Overexpression of $\beta$-Galactosidase in the Alkane-Utilizing Yeast Yarrowia lipolytica

  • Thomas Juretzek;Hui-Jie wang;Nicaud, Jean-Marc;Stephan Mauersberger;Gerold Barth
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제5권5호
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    • pp.320-326
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    • 2000
  • Promoters of the genes G3P, ICL1, POT1, POX1, POX2 and POX5 of the yeast Y. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was to select suitable promoters for high expression of heterologous genes in this yeast. For this purpose the promoters were fused with the reporter gene lacZ of E. coli and integrated as single copies into the genome of Y. lipolytica strain PO1d. The measurement of expressed activities of ${\beta}$-galactosidase revealed that pICL1, pPOX2 and pPOT1 are the strongest regulable promoters available for Y. lipolytica, at present. pPOX2 and pPOT1 were highly induced during growth on oleic acid and were completely repressed by glucose and glycerol. pICL1 was strongly inducible by ethanol besides alkanes and fatty acids, however, not completely repressible by glucose or glycerol. Ricinoleic acid methyl ester appeared as a very strong inducer for pPOT1 and pPOX2, in spite of that it inhibited growth of Y. lipolytica transformants.

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방출제어를 위한 잘토프로펜이 함유된 폴리옥살레이트 미립구의 제조와 특성 (Preparation and Characterization of Zaltoprofen-Loaded Polyoxalate Microspheres for Control Release)

  • 김경희;이천중;조선아;이정환;장지은;이동원;권순용;정진화;강길선
    • 폴리머
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    • 제37권6호
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    • pp.702-710
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    • 2013
  • 서방성방출을 위해 잘토프로펜을 함유한 미립구를 oil-in-water(O/W) 에멀젼용매 증발법을 이용하여 제조하였다. 제조온도, 교반속도, 초음파분쇄기의 강도, 약물농도, POX의 분자량과 농도, 유화제 농도 등의 제조조건에 따른 잘토프로펜의 방출거동을 평가하였다. 잘토프로펜을 함유한 POX 미립구의 물리화학적 성질 및 형태를 X선 회절분석법(XRD)과 시차주사열량계(DSC), 적외선 분광분석기(FTIR), 주사현미경(SEM)을 통해 연구하였다. 이러한 연구 결과로부터 제조조건들에 따른 미립구의 특성을 확인할 수 있었다. 또한 POX 미립구의 분해성을 10일 동안 in vitro 실험을 통해 조사하였다. 본 연구를 통해 잘토프로펜을 함유한 POX 미립구를 최적화된 용매증발방법으로 제조하였고, 이 미립구로부터 약물의 방출제어를 확인할 수 있었다.

Biochemical Changes Induced due to Staphylococcal Infection in Spongy Alphonso Mango(Mangifera indica L.) Fruits

  • Janave, Machhindra Tukaram
    • Journal of Crop Science and Biotechnology
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    • 제10권3호
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    • pp.167-174
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    • 2007
  • Spongy Alphonso mangoes were found to be infected with Staphylococcus bacteria. A Gram positive Staphylococcus strain was isolated from spongy pulp and identified from CABI Bioscience, UK, by partial 16S rDNA sequence analysis and by morphological and biochemical characterization through IMTECH, Chandigarh, India. Although identification by both of these methods indicated the organism belonged to same genus, different species names were given. Changes in total phenolics, reducing, and non-reducing sugars, respiration rate, total carotenoids, peroxidase(POX), and catalase activities were monitored during ripening of these fruits. The climacteric rise in spongy fruits was marked by an increase in respiration rate and a decrease in sugar content. Total phenolics content increased in spongy fruits as compared to ripe non-spongy fruits. Development of corky white tissue in spongy fruits was associated with about a 2.5-fold reduction in total carotenoids and a concomitant increase in lipoxygenase-mediated, $\beta$-carotene co-oxidation. A marked decrease in soluble protein content and about a 1.5-fold increase in POX activity was observed. Maximum POX activity was confined to 50-70%$(NH_4)_2SO_4$ fraction. The intense dark bands visible after POX specific substrate staining of the Native gel indicated a high expression of isoenzymes of POX in spongy fruits. Similarly, changes in levels of catalase activity were also observed in spongy fruits. The results suggest that infection of Alphonso mangoes with Staphylococcus bacteria affects the normal ripening processes of the fruit interfering with the carbohydrate and carotenoid metabolism. Also, the studies indicate the expression of POX and catalase enzymes as a plant defense response to microbial invasion.

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