• The Korean Journal of Ecology
• /
• v.23 no.2
• /
• pp.187-192
• /
• 2000

After Cassia mimosoides var. nomame was treated with Phytolacca americana extracts during the germination process, its effect on the activities of AsPOX and GuPOX were examined. As the concentration of P. americana extract increased. the activity of AsPOX decreased while that of GuPOX increased. During the germination process, the activity of AsPOX was lower than the control, while the activity of GuPOX was over 2 times higher than the control. Activity of total peroxidase by IEF was extremely high at pI 6.4 and pI 6.6 when treatment was made with a 30% concentration of P. americana extract. The treatment with phenolic compounds, caffeic acid and benzoic acid did not show much difference from the control although a slight increase was observed at pI 6.6. Activity of GuPOX in C. mimosoides was over 4 times higher in roots than in shoots. Namely, because GuPOX activity of C. mimosoides was increased by extracts of P. americana, defense enzyme, GuPOX. was generated against external stress, and we could certified the activity increase at pI 6.4, especially in root.

• Korean Journal of Veterinary Service
• /
• v.40 no.2
• /
• pp.149-153
• /
• 2017

The present study was carried out to evaluate the effect of avian pox on wild bird population by investigating the avian pox infection in migratory birds of a stopover site. 3,565 birds in 116 species were examined for avian pox in migratory birds at the Heuksando island in South Korea during the spring and fall of 2011. 20 birds in 12 species were found pox-like lesions and 5 birds were diagnosed by avian pox using PCR: Pale Thrush (Turdus pallidus), Yellow-breasted Bunting (Emberiza aureola), Yellow-throated Bunting (Emberiza elegans), Rustic Bunting (Emberiza rustica), Black-faced Bunting (Emberiza spodocephala). To our knowledge, this is the first report of avian pox detected in these 5 species of the world.

• Korean Journal of Veterinary Research
• /
• v.5 no.1
• /
• pp.37-41
• /
• 1965

For the preparation of an effective fowl pox vaccine, comparative studies of a number of fowl and pigeon pox virus strains were accomplished, and the following conclusions were made. 1. Anyang-Nakano strain which was nation widely used as a seed virus of fowl pox vaccine was proven its inadequacy. 2. A liquid vaccine prepared with Minnesota strain of pigeon pox virus showed its stability for 6 months and on side reaction.

• BMB Reports
• /
• v.29 no.6
• /
• pp.564-568
• /
• 1996

Bursts of ethylene production occurred in twice at an early exponential (EEP) and prestationary (PSP) phases, respectively, during growth of callus tissue isolated from the root of soybean seedlings. The second burst of ethylene production at PSP was smaller in magnitude than the earlier one at EEP, but was followed by increases in both guaiacol peroxidase (GuPOX) and ascorbate peroxidase (AsPOX). The increase in AsPOX activity was also preceded by an increase in lipoxygenase (LOX) activity. Treatment of the tissue with the ethylene antagonist 2,5-norbonadiene (NBD) resulted in substantial reduction in LOX and AsPOX activities during this period. GuPOX activity was reduced only slightly, if any, by NBD. Role of ethylene in the sequential induction of LOX and AsPOX in senescing callus tissue is discussed.

• Microbiology and Biotechnology Letters
• /
• v.21 no.2
• /
• pp.181-186
• /
• 1993

The functions of n-alkane inducible genes, ALI1 and POX18Cm isolated from Canida maltosa were investigated, using it's distruptants. As a result, it is suggested that ALI1 is essential for n-alkane assimilation in C. mltosa and it regulates genes related to assimilation of n-alkane (ALI1, P450alk POX18Cm) at transcriptional level. Nuclear localization experiments indicated that ALI1 was located and functioned in the nucleus. POX18Cm is considered as a peroxisomal nonspecific lipid transfer protein gene related to n-alkane assimilation in C. maltosa also regulated by ALI1. But it had no significant effect on n-alkane assimilation in C. maltosa.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.5
• /
• pp.320-326
• /
• 2000

Promoters of the genes G3P, ICL1, POT1, POX1, POX2 and POX5 of the yeast Y. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was to select suitable promoters for high expression of heterologous genes in this yeast. For this purpose the promoters were fused with the reporter gene lacZ of E. coli and integrated as single copies into the genome of Y. lipolytica strain PO1d. The measurement of expressed activities of ${\beta}$-galactosidase revealed that pICL1, pPOX2 and pPOT1 are the strongest regulable promoters available for Y. lipolytica, at present. pPOX2 and pPOT1 were highly induced during growth on oleic acid and were completely repressed by glucose and glycerol. pICL1 was strongly inducible by ethanol besides alkanes and fatty acids, however, not completely repressible by glucose or glycerol. Ricinoleic acid methyl ester appeared as a very strong inducer for pPOT1 and pPOX2, in spite of that it inhibited growth of Y. lipolytica transformants.

• Journal of the korean veterinary medical association
• /
• v.51 no.11
• /
• pp.651-653
• /
• 2015

• Polymer(Korea)
• /
• v.37 no.6
• /
• pp.702-710
• /
• 2013

Zaltoprofen loaded polyoxalate (POX) microspheres were prepared by an emulsion solvent-evaporation/extraction method like oil-in-water (O/W) for sustained release of zaltoprofen. The influence of several preparation parameters such as fabrication temperature, stirring speed, intensity of the sonication, initial drug ratio, molecular weight ($M_w$) of POX, concentration of POX and concentration of emulsifier has been investigated on the zaltoprofen release profiles. Physicochemical properties and morphology of zaltoprofen loaded POX microspheres were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimeter (DSC) and Fourier transform infrared (FTIR). Through the analyzed results, it was demonstrated that the characteristics of the microspheres greatly affected by the prepared condition. The releases behavior of zaltoprofen was investigated for 10 days in vitro. It was confirmed that the release behavior of zaltoprofen can be controlled by the manufacturing factor of solvent-evaporation/extraction method.

• Journal of the korean veterinary medical association
• /
• v.21 no.7
• /
• pp.410-412
• /
• 1985

The outbreak of swine pox from a herd of pigs was pathologically diagnosed in Korea. The skin lesions were those of a typical pox virus, pustules and crusts 1$\~$2 cm in diameter, and they affected mostly piglets 2$\~$3 months of age

• Journal of Crop Science and Biotechnology
• /
• v.10 no.3
• /
• pp.167-174
• /
• 2007

Spongy Alphonso mangoes were found to be infected with Staphylococcus bacteria. A Gram positive Staphylococcus strain was isolated from spongy pulp and identified from CABI Bioscience, UK, by partial 16S rDNA sequence analysis and by morphological and biochemical characterization through IMTECH, Chandigarh, India. Although identification by both of these methods indicated the organism belonged to same genus, different species names were given. Changes in total phenolics, reducing, and non-reducing sugars, respiration rate, total carotenoids, peroxidase(POX), and catalase activities were monitored during ripening of these fruits. The climacteric rise in spongy fruits was marked by an increase in respiration rate and a decrease in sugar content. Total phenolics content increased in spongy fruits as compared to ripe non-spongy fruits. Development of corky white tissue in spongy fruits was associated with about a 2.5-fold reduction in total carotenoids and a concomitant increase in lipoxygenase-mediated, $\beta$-carotene co-oxidation. A marked decrease in soluble protein content and about a 1.5-fold increase in POX activity was observed. Maximum POX activity was confined to 50-70%$(NH_4)_2SO_4$ fraction. The intense dark bands visible after POX specific substrate staining of the Native gel indicated a high expression of isoenzymes of POX in spongy fruits. Similarly, changes in levels of catalase activity were also observed in spongy fruits. The results suggest that infection of Alphonso mangoes with Staphylococcus bacteria affects the normal ripening processes of the fruit interfering with the carbohydrate and carotenoid metabolism. Also, the studies indicate the expression of POX and catalase enzymes as a plant defense response to microbial invasion.