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Physical Characteristics and Microsatellite Polymorphisms in Miryang Native Dogs (밀양지방 토종개의 형태학적 특징 및 유전적 다양성 연구)

  • Cho, Byung-Wook;Cho, Gil-Jae
    • Journal of Life Science
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    • v.16 no.4
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    • pp.626-631
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    • 2006
  • This study was carried out to investigate the physical characteristics (height, body lenght, chest depth, head type, ear type, body color, eye type and tail type) and genetic diversity using 15 microsatellite DNA markers (PEZ 1, 5, 8, 10, 11, 12, 13, 15, 16, 17, 20, 21, FHC 2010, FHC 2054 and FHC 2079) in 44 random Miryang native dogs(6 months${\sim}$12 years old). The height, body lenght, and chest depth of Miryang native dogs were 43-55 cm(mean 49.5 cm), 45-60 cm(mean 54.3 cm), and 50-64 cm(mean 57.9 cm), respectively. Miryang native dog was medium sized. The head and eye type were reverse-triangle(100%), triangle (90.9%) and newborn moon(9.1%), respectively. Most of body color had white coat color(93.2%), light pink tongue color(100%), light black anal color(90,9%) and pink claw color(100%). The ear type showed erect ear(100%), and half-curled(56.8%), upward(34.1%), curled(9.1%) in tail type, respectively. Number of alleles observed at a single locus ranged from 2 (PEZ 21 and FHC 2010) to 14 (PEZ 13), with average number of alleles per locus of 6.13. The expected heterozygosities of 15 microsatellite loci were estimated based on gene frequencies. The highest expected heterozygosity, 0.863 was estimated in PEZ 13 locus and the lowest, 0.455 in PEZ 21 and FHC 2010 locus. And the mean expected heterozygosity of 15 microsatellite markers was calculated as 0.635. Polymorphic information content (PIC) values were ranged from 0.348 (PEZ 21 and FHC 2010) to 0.837 (PEZ 13), and the mean PIC value was calculated as 0.570. Of the 15 markers, PEZ 10, PEZ 13, PEZ 17 and FHC 2054 loci have relatively high PIC value (> 0.7) in Miryang native dog. In order to determine the efficieney of parentage control, exclusion probabilities (PE) were calculated for each allele. The highest PE 1 and PE 2 in PEZ 13 locus was caculated to 0.548 and 0.710, respectively. And the total exclusion power in PE 1 and PE 2 was calculated to 0.9895 and 0.9996, respectively. These results can give basic information for perservation and research in Miryang native dog, and phylogenetic relationships of the Korean native dog and Asian dog breeds.

Characteristics of Ju-Back and Effect of Ju-Back Fertilizer on Growth of Crop Plants (주류생산 부산물인 주박의 특성 규명 및 주박이 작물생육에 미치는 영향)

  • Lee, Jung-Hoon;Park, Sung-Min;Park, Chi-Duck;Jung, Hyuck-Jun;Kim, Hyun-Soo;Yu, Tae-Shick
    • Journal of Life Science
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    • v.17 no.11
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    • pp.1562-1570
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    • 2007
  • This experiment was conducted to develop fertilizer which promotes plant growth as well as suppressing pathogenic fungi. The fertilizer was made from the mixture of Ju-Back (Korean rice wine cake) and indigenous rhizosphere-bacterium. The main ingredients of Ju-Back were investigated as 6.04% total nitrogen, 42.59% total carbohydrate, 1.01% available phosphate, 73.42% organic matter, 7.72% potassium oxide, 1.35% calcium oxide, 0.53% magnesium oxide. The enzyme activities of Ju-Back were estimated to be 980 units/g for ${\alpha}-amylase$, 300 units/g for glucoamylase, and 1800 units/g for acid pretense. Indigenous rhizosphere bacteria which produced antifungal agent were isolated from soil, and was selected KMU-13 strain which can antagonize against various plant pathogenic fungi (Botrytis cinerea KACC 40573, Sclerotinia sclerotiorum KACC 41065, Fusairum oxysporum KACC 40052, Pythium aphanidermatum KACC 40156, Phytophthora capsici KACC 40476 and Glomerella cingulata KACC 40299). KMU-13 strain was identified as Bacillus subtilis KMU-13 by biochemical and 16s rDNA analysis. The organic fertilizer was made as prototype which was composed 20% Ju-Back, 70% carrier, 9.7% microorganism cultivated solution, 0.3% trace-element. We also investigated an application of fertilizer using Ju-Back for cultivating lettuce (Lactuca sativar) which were grown in three soil conditions that had chemical fertilizer, barnyard manure, lime power, urea, potassium chloride and superphosphate as a control, the whole quantity (80 kg/10a) of posted fertilizer with the control and the half quantity (40 kg/10a) with the control. The growth characteristics were examined and analysed with several weeks interval from 3 weeks to 8 weeks on head length (cm), head width (cm/head), number of leaf and fresh weight (g/plant). The results are summarized as follows. The head width and fresh weight of lettuce were the highest at posted fertilizer 1 (whole quantity) was applied chemical, organic matter (Ju-Back) and carrier. The head length was the highest at posted fertilizer 2 (whole quantity) was applied Ju-Back only.

Effect of Alkaline Ionized Water on Stabilization of Antioxidation, Antithrombosis and Antibacterial Activities (항산화, 항혈전 및 항세균 활성의 안정화에 미치는 알칼리 이온수의 영향)

  • Ahn, Seon-Mi;Kang, Mee-A;Kim, Moo-In;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.20 no.7
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    • pp.1107-1112
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    • 2010
  • In this study, the stable maintenance of bioactivity in alkaline ionized water (AIW) and antibacterial effects of AIW were evaluated to confirm benefits of AIW. As controls, purified water (PW) and tap drinking water (DW) were used. The pH and ORP (oxidation-reduction potential) of AIW, PW and DW used were 9.5 and 120 mV, 7.2 and 144 mV, and 7.3 and 564 mV, respectively. High level of minerals was observed in DW (DW>AIW>PW of mineral contents). Concentrations of $Ca^{++}$ and $Na^+$ in DW were 14.5, and 8.4 mg/l, respectively, while no $Ca^{++}$, $Mg^{++}$, $K^+$, and $Na^+$ were detected in PW. Evaluation of antioxidant activities for AIW, PW and DW showed that the waters did not act as antioxidants. However, the DPPH (1,1-diphenyl-2-picryl hydrazyl) or superoxide radical scavenging activities or reducing power of vitamin C were stably maintained in AIW and PW, though not in DW, against heat treatment ($60^{\circ}C$) or vigorous shaking (120 rpm) at $37^{\circ}C$. Similarly, after aspirin treatment at $60^{\circ}C$ for 1 hr, the antithrombosis activity in PW and AIW was 62.6% and 55.3%, while that of DW was 52.1%. Furthermore, cell growth analysis and viable cell count of Escherichia coli H7:O157 in PW, AIW and DW showed that AIW and DW, not DW, have antibacterial activities. Our results suggest that the state of water, for example pH, ORP and mineral contents of water, should be considered in medicine or food industries, and that AIW has high potential for utilization in various fields.

A Study on Optimal Site Selection for Automatic Mountain Meteorology Observation System (AMOS): the Case of Honam and Jeju Areas (최적의 산악기상관측망 적정위치 선정 연구 - 호남·제주 권역을 대상으로)

  • Yoon, Sukhee;Won, Myoungsoo;Jang, Keunchang
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.4
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    • pp.208-220
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    • 2016
  • Automatic Mountain Meteorology Observation System (AMOS) is an important ingredient for several climatological and forest disaster prediction studies. In this study, we select the optimal sites for AMOS in the mountain areas of Honam and Jeju in order to prevent forest disasters such as forest fires and landslides. So, this study used spatial dataset such as national forest map, forest roads, hiking trails and 30m DEM(Digital Elevation Model) as well as forest risk map(forest fire and landslide), national AWS information to extract optimal site selection of AMOS. Technical methods for optimal site selection of the AMOS was the firstly used multifractal model, IDW interpolation, spatial redundancy for 2.5km AWS buffering analysis, and 200m buffering analysis by using ArcGIS. Secondly, optimal sites selected by spatial analysis were estimated site accessibility, observatory environment of solar power and wireless communication through field survey. The threshold score for the final selection of the sites have to be higher than 70 points in the field assessment. In the result, a total of 159 polygons in national forest map were extracted by the spatial analysis and a total of 64 secondary candidate sites were selected for the ridge and the top of the area using Google Earth. Finally, a total of 26 optimal sites were selected by quantitative assessment based on field survey. Our selection criteria will serve for the establishment of the AMOS network for the best observations of weather conditions in the national forests. The effective observation network may enhance the mountain weather observations, which leads to accurate prediction of forest disasters.

An Analysis of Papers used in Historical Manuscrips (조선시대 고문서(古文書)에 사용된 종이 분석)

  • Son, Ke-Young
    • Journal of Korean Society of Archives and Records Management
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    • v.5 no.1
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    • pp.79-105
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    • 2005
  • This study is that the name from Tagjijunjeol is compared with the actual papers used in historical documents. I have selected the families having had influencing power in Gyungi-Do(京畿道), Gyungsang-Do(慶尙道), Jeonra-Do(全羅道), Chungcheung-Do(忠淸道), and analyzed 1504 articles of historical documents owned by those families. I checked up each papers used in historical documents in it's shape of screens, density, raw material. I cannot find the feature of it's region, but prominent feature according to the types and period of historical documents. At first, I have surveyed the impressions of screen marks in papers. The number of strips can be divided into two cases, around 10 and around 17. The case around 17 is similar to the number seen in the book paper of the early Joseon Dynasty, but the case around 10 is rare. Dard Hunter said in his book, published in 1933, that screens were made of Korean grass(Miscanthus sp.) at that time in Korea. I guess the impressions of screen marks of the case around 10 was made of Korean grass, Miscanthus sp.. The case around 10 and the case around 17 were not different in period, but the contemporary appearance. However, if we look into the change of strips' number, we can learn that it becomes more dense as time goes by. The intervals of hair stitch, contrary to book paper, have appeared irregularly and a short hair stitch was frequently found between long hair stitches. This was the result of fastening extra stitch to increase the firmness of strips, as the part of screens which was frequently touched by hands would wear out easily. Then, have look into the density. Each kinds of historical documents had a wide range of density. But the average have had relationship with the kinds of paper written in Tagjijunjeol. The historical documents having the same item of paper have kept similar densities. From the density variation of Gosingyoji(告身敎旨), the letter of appointment the official rank over 5 by king, based on time periods, we can see a great disparity between before and after Imjin-waeran war. Before Imjin-waeran war paper's quality was high, right after Imjin-waeran war went abruptly down, and went gradually up just from 1650's, but since 1700's quality has not risen more, kept the value. On the contrary, the density of Gyocheop(敎牒), the letter of appointment the official rank under 5 by the Ministry of Personnel and the Ministry of Military, have not had a great disparity according to time periods, but had a lower density than Gosingyoji. Joseon Dynasty has strictly divided Gosingyoji appointing the official rank over 5 from Gyocheop appointing the official rank under 5, it seems to distinguish the appointment by king from the appointment by Ijo(吏曹), the Ministry of Personnel and Byeongjo(兵曹), the Ministry of Military.

Recovery of $SF_6$ gas from Gaseous Mixture ($SF_6/N_2/O_2/CF_4$) through Polymeric Membranes (고분자 분리막을 이용한 혼합가스($SF_6/N_2/O_2/CF_4$)로부터 $SF_6$의 회수)

  • Lee, Hyun-Jung;Lee, Min-Woo;Lee, Hyun-Kyung;Choi, Ho-Sang;Lee, Sang-Hyup
    • Membrane Journal
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    • v.21 no.1
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    • pp.22-29
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    • 2011
  • During the maintenance, repair and replacement process of circuit breaker, $SF_6$ reacted with input air in arc discharge, which led to the production of by-product gases (eg, $N_2$, $O_2$, $CF_4$, $SO_2$, $H_2O$, HF, $SOF_2$, $CuF_2$, $WO_3$). Among these various by-product gases, $N_2$, $O_2$, $CF_4$ is major component. Therefore, the effective separation process is necessary to recycle the $SF_6$ gas from the mixture gas containing $N_2$, $O_2$, $CF_4$. In this study, the membrane separation process was applied to recycle the $SF_6$ gas from the mixture gas containing $N_2$, $O_2$, $CF_4$. The concentration of $SF_6$ gas in gas produced from the electric power industry is over than 90 vol%. Therefore, we made the simulated gas containing $N_2$, $O_2$, $CF_4$, $SF_6$ which the concentration of $SF_6$ gas is minimum 90 vol%. From the results of membrane separation process of $SF_6$ gas from $N_2$, $O_2$, $CF_4$ $SF_6$ mixture gases, PSF membrane shown the highest recovery efficiency 92.7%, in $25^{\circ}C$ and 150 cc/min of retentate flow rate. On the other hand, PC membrane shown the highest recovery efficiency 74.8%, in $45^{\circ}C$ and 150 cc/min of retentate flow rate. Also, the highest rejection rate of $N_2$, $O_2$, $CF_4$ is 80, 74 and 58.9% seperately in the same operation condition of highest recovery efficiency. From the results, we supposed the membrane separation process as the effective $SF_6$ separation and recycle process from the mixture gas containing $N_2$, $O_2$, $CF_4$, $SF_6$.

Selectivity and Permeability Characteristics of Pure CO2 and N2 Gases through Plasma Treated Polystyrene Membrane (플라즈마 처리된 폴리스티렌 막을 통한 순수한 CO2 와 N2 기체의 선택·투과 특성)

  • Hwang, Yui-Dong;Shin, Hee-Yong;Kwak, Hyun;Bae, Seong-Youl
    • Korean Chemical Engineering Research
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    • v.44 no.6
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    • pp.588-596
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    • 2006
  • The surface of polystyrene membrane treated by Ar, $O_2$ plasma, and the effects were observed before and after the treatment and permeability of $CO_2$, $N_2$ and selectivity of $CO_2$ relative to $N_2$ was measured using continuous flow gas permeation analyzer (GPA). The mole ratio of O over C in the surface was increased from 0 to 0.179 with Ar plasma treatment and route mean square of surface was increased from $15.86{\AA}$ to $71.64{\AA}$. Therefore the contact angle was decreased from $89.16^{\circ}$ to $18.1^{\circ}$. Thus Plasma treatments made surface of membrane tend to be highly hydrophilic. The optimum condition for the $CO_2$ permeability and ideal selectivity of the plasma treated membrane was as follows: the measurement of Ar (60 W, 2 min, $70^{\circ}C$) plasma treatment was $1.14{\times}10^{-12}[m^3(STP){\cdot}m/m^2{\cdot}sec{\cdot}atm]$ and 4.22. In the case of $O_2$ plasma treatment, the contact angle was decreased at $13.56^{\circ}$ with increase of O/C ratio ($0.189{\AA}$) and route mean square of surface ($57.10{\AA}$). The optimum condition for the $CO_2$ permeability and ideal selectivity of the plasma treated membrane was as follows: the measurement of $O_2$ (90 W, 2 min, $70^{\circ}C$) plasma treatment was $7.1{\times}10^{-12}[m^3(STP){\cdot}m/m^2{\cdot}sec{\cdot}atm]$ and 11.5. After plasma treatment, the changes of membrane surface were all subtly linked with both cross-linking and etching effects. Finally, it was confirmed that the gas permeation capacity and selectivity of the modified membrane with plasma could be improved by an appropriate control of the plasma conditions such as treatment time, the power input and sort of plasma gas.

Process Suggestion and HAZOP Analysis for CQ4 and Q2O in Nuclear Fusion Exhaust Gas (핵융합 배가스 중 CQ4와 Q2O 처리공정 제안 및 HAZOP 분석)

  • Jung, Woo-Chan;Jung, Pil-Kap;Kim, Joung-Won;Moon, Hung-Man;Chang, Min-Ho;Yun, Sei-Hun;Woo, In-Sung
    • Korean Chemical Engineering Research
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    • v.56 no.2
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    • pp.169-175
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    • 2018
  • This study deals with a process for the recovery of hydrogen isotopes from methane ($CQ_4$) and water ($Q_2O$) containing tritium in the nuclear fusion exhaust gas (Q is Hydrogen, Deuterium, Tritium). Steam Methane Reforming and Water Gas Shift reactions are used to convert $CQ_4$ and $Q_2O$ to $Q_2$ and the produced $Q_2$ is recovered by the subsequent Pd membrane. In this study, one circulation loop consisting of catalytic reactor, Pd membrane, and circulation pump was applied to recover H components from $CH_4$ and $H_2O$, one of $CQ_4$ and $Q_2O$. The conversion of $CH_4$ and $H_2O$ was measured by varying the catalytic reaction temperature and the circulating flow rate. $CH_4$ conversion was 99% or more at the catalytic reaction temperature of $650^{\circ}C$ and the circulating flow rate of 2.0 L/min. $H_2O$ conversion was 96% or more at the catalytic reaction temperature of $375^{\circ}C$ and the circulating flow rate of 1.8 L/min. In addition, the amount of $CQ_4$ generated by Korean Demonstration Fusion Power Plant (K-DEMO) in the future was predicted. Then, the treatment process for the $CQ_4$ was proposed and HAZOP (hazard and operability) analysis was conducted to identify the risk factors and operation problems of the process.

A Study on Advancing Strategy for National Environmental Geographic Informations - Focused on the National Environmental Assessment Map, Ecological Map and Land Cover Map - (국토환경지리정보 고도화 전략 연구 - 국토환경성평가지도, 생태자연도, 토지피복지도를 중심으로 -)

  • Lee, Chong-Soo
    • Journal of Environmental Policy
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    • v.6 no.2
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    • pp.97-122
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    • 2007
  • In 2006, the Ministry of Environment of the Republic of Korea, completed the construction on national environmental geographic informations including National Environmental Assessment Map, Ecological Map, Land Cover Map and so on. At this point of time, it is necessary to establish the advance strategy on national environmental geographic information, considering the complicated characteristics. Therefore, this study suggests the advance strategy on national environmental geographic information, reflecting results of analyzing the given condition and the trend of informatization. National environmental geographic information has spacial quality to be managed dispersedly in a department unit or an operations unit. According to this quality, requirements for users who need the policy based on national environmental geographic information and complex information are not satisfactory. And, the information system centering the process of administrative affairs should be converted to one putting decision supporting first in importance. Therefore, this study sets up "the realization of the sustainable land management system by advancing national environmental geographic information" as the vision of the advancing strategy. In order to accomplish the vision, this study established the purpose as follow; constructing strategic and geographic information based on knowledge, arranging the foundation to open information to the public transparently, building expanded and integrated national environmental geographic information, embodying the environmental administration based on national environmental geographic information, enhancing the efficiency of national environmental geographic information, and supporting efficiently the process of administrative affairs. And this study suggests executive plans to achieve the vision and the purpose as next; developing the quality control program to verify the information confidence, building the system to integrate and to provide environmental information, collecting information, readjusting laws and regimes in parts of the construction, the application and the management of the system, and operating the task process, human power, organization and information technology. This study puts the emphasis on providing the turning opportunity politically which is possible to make sure of the information confidence in quality, advancing from the expansion of one in quantity. However, this improvement strategy doesn't reflect all national environmental geographic information and current status of environmental administrations. Therefore, for applying the result of this study to the actual environmental administration, it is necessary to discuss regularly the systematic categorization of national environmental geographic information, to interview with the contracting parties and so on hereafter.

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Fermentation Characteristics of Bread Added with Pleurotus eryngii Powder (새송이버섯 분말을 첨가한 식빵의 발효특성)

  • Lee, Ji-Yeon;Lee, Kyung-Ae;Kwak, Eun-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.6
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    • pp.757-765
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    • 2009
  • Bread was prepared with different concentrations of Pleurotus eryngii powder ($2.5{\sim}10.0%$), and the fermentation characteristics were investigated. While pH of dough increased, fermentation power of dough expansion by fermentation decreased with the increase of Pleurotus eryngii powder added. Although bread weight decreased, there were no significant differences in dough weight, dough yield and baking loss as Pleurotus eryngii powder content increased. In case of volume and specific volume, they were significantly decreased as Pleurotus eryngii powder content increased. Size of bread decreased gradually and aircell uniformity became bigger and random as Pleurotus eryngii powder content increased. As the results of color values, L and b values of crust decreased; however, there was no significant difference in a value. L value of crumb decreased although a and b values increased by adding the Pleurotus eryngii powder content. In the textual characteristics, hardness, gumminess and brittleness of bread significantly increased as Pleurotus eryngii powder content increased. Cohesiveness and springiness of bread decreased as Pleurotus eryngii powder content increased. In case of free amino acid, Glu content was the largest in most samples and most amino acid contents increased by adding the Pleurotus eryngii powder content except for Asp. In descriptive test, scores of color, flavor, hardness and palatability increased, however aircell uniformity, springiness and moistness decreased as Pleurotus eryngii powder content increased. As the results of preference test, color was the best preferred in control${\sim}5.0%$ groups without significant difference. The preference of flavor and texture was in the order of 2.5% group> 5.0% group> 7.5% group> control> 10.0% group as 2.5% group was the most preferred.