• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.287-293
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• 2010

Reliable discrimination of a single nucleotide mismatch was demonstrated using arrays with peptide nucleic acid (PNA) probes. The newly developed PNA probes immobilization method and hybridization conditions for PNA arrays gave excellent specificity and sensitivity. In addition we compared the specificity, sensitivity, and stability obtained with the PNA and DNA arrays in discriminating single nucleotide mismatches. The PNA arrays had superior perfect match-to-mismatch signal ratios and sensitivities. The relative signal intensities of mismatch PNA probes ranged from 1.6% to 12.1% of the perfect-match PNA probes. These results demonstrated that the PNA arrays were 2.0 to 37.3 times more specific and about 10 times more sensitive than DNA arrays. The PNA array showed the same specificity and sensitivity after 12-month storage at room temperature.

• 한국미생물·생명공학회지
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• 제47권4호
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• pp.645-650
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• 2019

Despite differences in virulence between strains of Toxoplasma gondii, rapid and accurate genotyping methods are lacking. In this study, a method was developed to detect and genotype T. gondii in food and environmental samples using PCR and a novel peptide nucleic acid (PNA) melting array. An alignment of genome sequences for T. gondii type I, II, and III obtained from NCBI was generated, and a single nucleotide polymorphism analysis was performed to identify targets for PCR amplification and a PNA melting array. Prior to the PNA melting array, conventional PCR was used to amplify GRA6 of T. gondii. After amplification, the PNA melting array was performed using two different PNA hybridization probes with fluorescent labels (FAM and HEX) and quenchers. Melting curves for each probe were used to determine genotypes and identify mutations. A 214-bp region of the GRA6 gene of T. gondii was successfully amplified by PCR. For all T. gondii strains (type I, II, and III) used to evaluate specificity, the correct genotypes were determined by the PNA melting array. Non-T. gondii strains, including 14 foodborne pathogens and 3 protozoan parasites, such as Giardia lamblia, Cryptosporidium parvum, and Entamoeba histolytica, showed no signal, suggesting that the assay has a high specificity. Although this is only a proof-of-concept study, the assay is promising for the fast and reliable genotyping of T. gondii from food and environmental samples.

• 한국전자파학회논문지
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• 제22권12호
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• pp.1155-1164
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• 2011

본 논문에서는 국내 최초로 개발되는 함상용 중거리 레이더에 사용되는 능동 위상 배열 안테나의 송신 빔 특성을 근접 전계 시험으로 측정하는 방안에 대하여 제시하였다. 측정하고자 하는 능동 위상 배열 안테나는 고출력 송신 빔을 펄스 형태로 방사하기 때문에 저출력의 연속적인 파형을 사용하는 일반적인 근접 전계 시험 시설에서는 측정하기 어렵다. 그래서 본 논문에서는 고출력 송신 시에도 견딜 수 있도록 설계된 근접 전계 시험 시설과 펄스 모드 측정을 지원하는 Agilent사의 PNA-X 네트웍 분석기를 이용한 근접 전계 시험으로 펄스 형태의 고출력 송신 빔을 방사하는 능동 위상 배열 안테나를 측정하는 방안에 대하여 제시하였고, 실제 개발된 능동 위상 배열 안테나의 고출력 송신 빔 패턴을 근접 전계 시험으로 측정하였다. 또한, 능동 위상 배열 안테나의 송신 특성인 EIRP(Effective Isotropic Radiated Power)를 측정하였고, 수학적인 계산을 통해 예측한 EIRP 값과 비교한 결과 0.1 dB의 오차 내에서 동일함을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제32권spc8호
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• pp.2895-2896
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• 2011

• Gut and Liver
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• 제12권6호
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• pp.641-647
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• 2018

Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (${\kappa}$-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.