• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.8
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• pp.1012-1017
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• 2017

Mesembryanthemum crystallinum (Family: Aizoaceae) is an annual plant consisting of ice crystal-shaped bladder cells, which is responsible for its common name ice plant. This study investigated biological activities according to general components and extraction solvent in order to examine the functionality of ice plant. The total content of free amino acids was 32.57 mg/g, including 4.64 mg/g of L-alanine as the most abundant and 2.60 mg/g of ${\gamma}$-aminobutyric acid. Regarding angiotensin converting enzyme inhibitory activities of solvent fractions of ice plant, ethyl acetate fraction and chloroform fraction showed activities of $33.17{\pm}3.20{\sim}88.19{\pm}3.20%$ and $23.72{\pm}2.89{\sim}86.78{\pm}2.24%$, respectively, similar to $Captopril^{(R)}$ ($19.51{\pm}3.44{\sim}84.72{\pm}1.06%$) and $Enalapril^{(R)}$ ($24.93{\pm}1.12{\sim}91.32{\pm}3.62%$) as positive control groups. Regarding inhibition of lipid droplet production in 3T3-L1 preadipocytes by ice plant, anti-adipogenic activities were $53.00{\pm}0.45{\sim}65.75{\pm}0.31%$ and $44.16{\pm}0.29{\sim}63.32{\pm}0.36%$ in the ethyl acetate fraction and butanol fraction, respectively, showing the lowest lipid droplet production. The chloroform fraction and hexane fraction showed activities of $38.33{\pm}0.09{\sim}56.55{\pm}0.50%$ and $31.17{\pm}0.50{\sim}55.10{\pm}1.93%$, respectively, whereas the water fraction showed activity of $26.32{\pm}2.27{\sim}49.48{\pm}0.05%$. Therefore, all solvent fractions inhibited fat accumulation of 3T3-L1 preadipocytes according to treatment concentration. According to the results above, it would be possible to utilize ice plant as a new health functional material.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.126-130
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• 2005

Manufacturing method and ingredient ratio for young radish kimchi (yulmoo kimchi) and young radish watery kimchi (yulmoo mool-kimchi) were standardized from literatures. Ingredients having frequency of use greater than 50% were only used in the standardization process. Green onion, red pepper, red pepper powder, garlic, ginger, and anchovy juice were included in young radish kimchi. Green pepper, red pepper, garlic, ginger, and starch were included in young radish watery kimchi. The standardized ingredients ratio of young radish kimchi (yulmoo kimchi) on young radish 100 g was as follows: green onion 8.0$\pm$3.8, crushed garlic 2.9$\pm$1.3, crushed ginger 1.6$\pm$0.7, red pepper 7.0$\pm$1.7, red pepper powder 4.2$\pm$1.2, and anchovy juice 3.7$\pm$0.5. The standardized ingredients ratio of young radish watery kimchi (yulmoo mool-kimchi) on added water 100 mL was as follows: young radish 50.6:$\pm$10.8, crushed garlic 3.0$\pm$0.7, crushed ginger $1.5\pm$0, green onion 3.3$\pm$1.3, green pepper 3.3$\pm$1.9, red pepper 2.4$\pm$1.3, and starch $1.5\pm$0.6.

• Korean Journal of Animal Reproduction
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• v.24 no.3
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• pp.241-248
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• 2000

In this study, we examined the number and motility of sperms, and also observed the changes in testes weight, and histological changes of several organs after 5 days of a continuous administration of dioxin (2,3,7,8-tetrachlorodibenzo-p-dioxin) of per oral administration of a herb drug extracts, which were administerated altermate days, to elucidate the effects of the a herb drug extracts on reproductive toxicity of dioxin. 1. The sperm numbers of dioxin-administered groups were 90.7$\pm$3.6~l18.5$\pm$3.6$\times$10/suup 6/$m\ell$ and 67.3$\pm$4.1~88.2$\pm$3.3$\times$10$^{6}$ $m\ell$ for 10~20 $\mu\textrm{g}$/kg and 30~40 $\mu\textrm{g}$/kg dosages of dioxin-administerated groups, respectively. Each dioxin-administered group showed prominently lower value than that of control group's which was 119.3$\pm$3.4~120.2$\pm$4.7 $\times$ 10$^{6.}$$m\ell$. 2. The sperm motility of each dioxin-administered group's also showed lower value than that of control group's which was 93.6$\pm$3.8~94.9$\pm$3.4%. The sperm motility of each dioxin-administerated group were 77.0$\pm$4.7~89.5$\pm$3.6% and 66.5$\pm$3.3 ~79.9$\pm$3.8% for 10~20 $\mu\textrm{g}$/kg and 30~40 $\mu\textrm{g}$/kg dosages of dioxin-administerated groups, respectively. 3. The sperm numbers of each group, which was administered a herb drug extracts, were 77.4$\pm$3.2~90.9$\pm$3.4$\times$10$^{6}$ $m\ell$, 78.0$\pm$3.3~105.0$\pm$4.2$\times$10$^{6}$ $m\ell$, 76.2$\pm$2.8~84.4$\pm$3.5$\times$10$^{6}$ $m\ell$ and 75.4$\pm$3.3~80.2$\pm$3.3$\times$10$^{6}$ $m\ell$ for extracts of green leaf, red ginseng, Kugija and Oume-administered groups respectively. And the sperm motility of each group were 63.4$\pm$3.8~77.0$\pm$4.0%, 65.5$\pm$4.1~87.4$\pm$3.8%, 64.3$\pm$4.2~69.8$\pm$4.2%, 66.3$\pm$3.9~66.0$\pm$4.0% for extracts of green leaf, red ginseng, Kugija and Oume extracts-administered groups, respectively. 4. The number and motility of sperm of control group were 119.3$\pm$3.4~120.2$\pm$4.7$\times$10$^{6}$ $m\ell$ and 93.0$\pm$3.5~96.1$\pm$3.5%, respectively. Red ginseng extracts- administered group seemed to be recovered than my other groups, and the green leaf extracts-administered group was shown to be the next. The Kugija and Oume extracts-administered groups didn't show to be recovered much. 5. Most a herb drug extracts-administered group except the red ginseng-administered group displayed prominently lower values of testes weights than that of control group's which was 0.15$\pm$0.01~0.16$\pm$0.01g. The red ginseng extracts-administered group seemed to be recovered conspicuously. 6. After 5 days of a continuous administration of 30 $\mu\textrm{g}$/kg dosage of dioxin followed by 3 weeks of per oral administration of green leaf, red ginseng, Kugija, or Oume extracts, histological findings showed that the liver, spleen, and testis of most a herb drug extracts-administered groups were damaged severely. By the way, the testes of red ginseng extracts-administered group seemed to recover compared to the other group's.ompared to the other group's.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.716-723
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• 1993

This study was carried out to estimate intake level of Pb, Fe, Cu in rural area of Korea(12 males and 18 females). Analyses for the nutritional status of the record, duplicated diet collections, 24-hour urine collections, and venous blood sampling before measuring of blood pressure. The mean age and blood pressure of the subjects were 45.8$\pm$11.1years and 117.5$\pm$22.2/80.8$\pm$15.6mmHe in males, and 41.9$\pm$11.0years, 110.0$\pm$ 11.9/73.9$\pm$8.5mmHg in females, respectively. In respect to both males and females, mean BMI were 22.0$\pm$2.3, 23.1$\pm$3.0, mean Rohrer index were 131.8$\pm$14.8, 142.4$\pm$20.2 and mean skinfold thickness were 12.4$\pm$5.9mm, 25.3$\pm$7.4mm, respectively. The daily mean intakes of Pb, Fe, and Cu estimated for 3 days were 277.2$\pm$111.2$\mu\textrm{g}$/day, 12.7$\mu\textrm{g}$ 7.6mg/day, and 3.0$\pm$1.4mg/day in males and 192.0$\pm$72.4$\mu\textrm{g}$/day, 13.3$\pm$5.8mg/day, and 3.7 $\pm$1.7mg/day in females, respectively. The mean concentrations of serum Pb, Fe, Cu were 11.0$\pm$2.0$\mu\textrm{g}$/dl, 131.1$\pm$42.6$\mu\textrm{g}$/dl, 120.3$\pm$25.7$\mu\textrm{g}$/dl in males and 9.0$\pm$1.2$\mu\textrm{g}$/dl, 112.2$\pm$ 35.9$\mu\textrm{g}$/dl, 117.3$\pm$17.9$\mu\textrm{g}$/dl in females, respectively. The mean levels of Hb and Hct were 15.1$\pm$1.1g/dl, 45.2$\pm$3.3% in males and 13.1$\pm$0.8g/dl, 39.8$\pm$2.6% in females, respectively. The 24-hour urinary excretions of Pb, Fe, Cu were 35.5$\pm$10.0$\mu\textrm{g}$/day, 0.16$\pm$0.12mg/day, 60.12$\pm$0.02$\mu\textrm{g}$/day in males, and 25.3$\pm$11.0$\mu\textrm{g}$/day, 0.24$\pm$0.20mg/day, 70.07$\pm$0.03$\mu\textrm{g}$/day in females, respectively. In conclusion, the Pb intake in self selected diet of this subjects was not in the level that antagonized to Fe and Cu metabolism seriously.

• Journal of the Korean Wood Science and Technology
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• v.15 no.2
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• pp.26-52
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• 1987

Among the diffuse-porous woods which arc dominant in Korea and used as construction materials due to their wood quality, ten species of six genus involving seven species of three genus in Betulaceae were studied on the radial variation of structural demenb. The species studied were Betula platyphylla var. japonica, B. ermanii, B. davurica, B. scstata, B. schmidtii, Carpinus laxifora, Alnus japonica, Prunus sargentii. Acer mono and Diospyros kaki. Wood fiber, vessel elements and ray increased rapidly in size from pith to a certain annual ring. After then the radial variation in size of the main structural elements seemed to be divided into three types; levelled off curve pattern indicating constant size(type I), continuously increasing curve pattern showing ever increase in size (type II) and parabolic curve pattern showing the gradual decrease after the maximum (type III), but the variation types by structural dements were different even in the same species. Based on the results from this study, it appears to be reasonable to consider the stabilized age of wood fiber, vessel elements and ray rather than considering wood fiber length in distinguishing mature woods from juvenile woods.

• Journal of Society of Preventive Korean Medicine
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• v.3 no.1
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• pp.36-36
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• 1999

From January 1994 to April 1997, there was 15 survivals who was admitted to the department of internal medicine, Wonkwang Oriental Medicine' Hospital in Cheun-ju, after ingestion of paraquat, and treated with Oriental and western medicine therapy. For the patients, I investigated clinical symptoms, gastroduodenoscopy, intake by oral and parenteral route, and output by urine and stool, serum ALP, AST. ALT, Bilirubin, BUN, Creatinine level and urine analysis. On admission day, the LFT level was as follows. The serum mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin was 10.05${\pm}$2.75 KAU, 66.67${\pm}$9.88 IU／L, 43.80${\pm}$7.74 IU／L, 1.89${\pm}$1.22 ㎎／dl and 1.10${\pm}$1.14 ㎎／dl respectively. After that day, administered Gamdutang and checked the mean LFT level regullary. Until the 3rd day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was 11.0l${\pm}$3.16 KAU, 56.47${\pm}$7.19 IU／L, 59.00${\pm}$7.57 IU／L, 2.54${\pm}$1.78 ㎎／dl, 1.64${\pm}$1.59 ㎎／dl respectively. From 4th day to 7th day, the mean ALP; AST, ALT, Total Bilirubin and Direct Bilirubin level was 12.51${\pm}$3.49 KAU, 77.85${\pm}$7.17 IU／L, 58.00${\pm}$9.09 IU／L, 2.54${\pm}$1.97 ㎎／dl, and 1.80${\pm}$1.81 ㎎／dl respectively. From 8th day to 10th day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was 12.43${\pm}$3.14 KAU, 41.13${\pm}$6.49 IU／L, 50.40${\pm}$7.17 IU／L, 1.66${\pm}$1.90 ㎎／dl and 1.14${\pm}$1.50 ㎎／dl respectively. From 11th day to 14th day, the mean ALP, AST, ALT, Total Bilirubin and Direct Bilirubin level was 12.30${\pm}$3.25 KAU, 31.07${\pm}$3.85 IU／L, 43.33${\pm}$5.49 IU／L, 1.62${\pm}$1.95 ㎎／dl, 1.17${\pm}$1.71 ㎎／dl respectvely. On admission day, the mean RFT level as follows. Serum BUN and Creatinine level was 28.73${\pm}$5.19 ㎎／dl and 1.82${\pm}$1.27 ㎎／dl respectively. After that day, administered Gamdutang and checked the mean RFT level regullary. Until the 3rd day, the mean BUN and Creatinine level was 32.12${\pm}$5.65 ㎎／dl and 2.31${\pm}$0.45 ㎎／dl respectively. From 4th day to 7th day, the mean BUN and Creatinine level was 31.07${\pm}$5.47 ㎎／dl and 1.92${\pm}$0.79 ㎎／dl respectively. From 7th day to 10th day, the mean BUN and Creatinine level was 17.47${\pm}$3.57 ㎎／dl and 1.33${\pm}$0.59 ㎎／dl respectively. From 11th day to 14th day, the mean BUN and Creatinine level was 11,93${\pm}$3.16 ㎎／dl, 1.27${\pm}$0.38 ㎎／dl respectively.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.3
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• pp.183-191
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• 2005

This study is designed to compare the performance of established samplers (personal air sampler and MiniVOL portable air sampler) commonly used in the air environment or work environment with that of the sampler made by remodeling the air bubble generator for aquarium fishes. Sampling method used in this study is the filter collection method for PM10 and total suspended particles (TSP), the liquid collection method for sulfur dioxide ($SO_2$) and nitrogen dioxide ($NO_2$), and the solid collection method for toluene, respectively. There is not a significant difference in the average concentration of TSP between the Gilian personal air sampler (1st, $0.316{\pm}0.095$; 2nd $0.191{\pm}0.090$; 3rd, $0.185{\pm}0.073mg/m^3$) and the remodeled sampler (1st, $0.317{\pm}0.106$, 2nd $0.201{\pm}0.050$; 3rd $0.189{\pm}0.081mg/m^3$). There are also not significant differences in the average concentration of PM10 among the Gilian personal air sampler ($0.058{\pm}0.006mg/m^3$), the remodeled sampler ($0.052{\pm}0.008mg/m^3$) and the MiniVOL portable air sampler ($0.054{\pm}0.007mg/m^3$). The average concentration of the SO2 by the established sampler and the remodeled one is $3.79{\pm}0.21ppb$ and $3.45{\pm}0.15ppb$, respectively. In addition, there are not sigmficant differences in the average concentration of the NO2 between the Gilian personal air sampler (1st, $0.325{\pm}0.068$; 2nd $0.341{\pm}0.206$; 3rd, $2.971{\pm}0.078{\mu}g/m^3$) and the remodeled sampler (1st, $0.300{\pm}0.062$; 2nd $0.332{\pm}0.144$, 3rd, $2.968{\pm}0.085{\mu}g/m^3$). There are not significant differences in the average concentration of toluene between the Gilian personal air sampler (1st, $0.499{\pm}0.072$; 2nd $0.598{\pm}0.112$; 3rd $2.284{\pm}0.077{\mu}g/m^3$) and the remodeled sampler (1st, $0.463{\pm}0.133$; 2nd $0.603{\pm}0.082$; 3rd $2.353{\pm}0.115{\mu}g/m^3$). From these results, we can conclude that the performance of the remodeled sampler is not different from that of established samplers. There is possibility that the remodeled sampler can be used as a alternative device for Gilian personal air sampler in area and personal air sampling.

• Journal of Life Science
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• v.18 no.12
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• pp.1728-1732
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• 2008

This study was performed to investigate the bionomical characteristics of Luciola lateralis in Korea. Imago's longevity of female was $17.5{\pm}0.7$ and male was $20.6{\pm}1.0$ days at $23^{\circ}C$, R.H. 80%, and significant difference was not occurred in breeding density. Pre-oviposition period was a pair $2.8{\pm}1.5$, two pairs $2.5{\pm}1.5$, three pairs $1.7{\pm}0.7$, four pairs $1.3{\pm}0.5$ days and pre-oviposition period was shorten in higher breeding density. The mean of laying eggs was $248.9{\pm}80.7$, was not related breeding density. Daily oviposition rate was 1st day 20.8%, 2nd 17.3%, 3rd 14.3%, 4th 12.7%, 5th 7.0%, the rate was highest in 1st day and gradually reduced. Egg period was $21.8{\pm}0.7$ days and hatchability was $95.5{\pm}4.8%$. Larval instar was identified the 9th instar, the evidence was able to its skin and marking pattern. Larval period of each instar was 1st $1.0{\pm}0.1$, 2nd $3.3{\pm}0.2$, 3rd $10.2{\pm}1.0$, 4th $6.8{\pm}0.5$, 5th $6.9{\pm}2.0$, 6th $15.4{\pm}4.1$, 7th $18.3{\pm}5.9$, 8th $25.8{\pm}8.7$, 9th $31.2{\pm}13.2$ days. Matured 9th larva was made of cocoon during $2.8{\pm}0.8$ days and pre-pupal, pupal period was $4.6{\pm}0.9$, $5.6{\pm}0.7$ days. Imago was made hard hind wing during $3.8{\pm}0.4$ days in cocoon and come out.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.229-234
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• 2002

This study was carried out to investigate the general characteristics such as concentration sperm motility and abnormality of sperm on the whole epididymal semen(EWS), RSP-S(removed seminal plasma by saline) and RSP-T(removed seminal plasma by tris-buffer) semen and survival rates after freezing on motility of whole and RSP-S and RSP-T semen and extender containing 2~8% glycerol, and ability of frozen-thawed sperm to penetrate homologous oocytes. 1. The concentration, motility and abnormality of epididymal WES, RSP-S and RSP-T sperm were 4.25 $\pm$ 0.25($\times$10$^{6}$ Cells/$m\ell$), 3.85$\pm$0.20($\times$10$^{6}$ Cells/$m\ell$), 4.05 $\pm$ 0.28($\times$10$^{6}$ Cells/$m\ell$), 50.55 $\pm$ 2.75%, 67.25 $\pm$ 2.55%, 78.75 $\pm$ 3.55 and 9.45 $\pm$ 2.25%, 37.75 $\pm$ 2.10%, 24.25 $\pm$ 1.55%, respectively. 2. The survival rates of slow and rapid frozen epididymal RSP-S and RSP-T sperm were 35.00 $\pm$ 2.35%, 45.50 $\pm$ 2.15％ and 16.50 $\pm$ 3.55%, 22.55 $\pm$ 3.95%, respectively. The survival rate of epididymal WES and RSP-T sperm after freezing following dilution with tris-buffer containing 2~8% glycerol were 9.25 $\pm$ 1.55%~17.50 $\pm$ 2.50%. 3. The percentage of capacitated and acrosome-reacted sperm prier to culture for fresh and frozen -thawed epididymal RSP-T semen were 45.25 $\pm$ 5.75%, 7.06 $\pm$ 0.25%, 48.20 $\pm$ 6.80% and 13.00 $\pm$ 2.35%, 3.55 $\pm$ 0.85%, 15.50 $\pm$ 1.90%, respectively. The penetration rate the number of sperm per penetrated for fresh and frozen-thawed epididymal RSP-T sperm were 39.25 $\pm$ 4.72%, 34.24 $\pm$ 3.93% and 1.30 $\pm$ 0.33, 1.10 $\pm$ 0.50., respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.4
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• pp.295-304
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• 2015

To develop a technique for efficient management of fertility for cucumber in greenhouse, a quick test method to quantify nitrate ($NO_3{^-}$) content in soil solution and leaf petiole juice using a simple instrument that are easy to use for farmers was investigated. N fertilizer (urea) was applied at 0, 50, 100 and 200% levels of the recommended application rate from 30 days after transplanting to harvest by soil fertigation treatments. Stable results were obtained from analysis of nitrate ($NO_3{^-}$) using top $10^{th}$ or $11^{th}$ leaf petioles collected between 10 to 11 am in the morning. Under the semiforcing culture, $NO_3{^-}$ content of leaf petiole juice was highest at 60 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$content of leaf petiole juice was $2,418{\pm}78{\sim}2,668{\pm}118$ at 45 DAT, $3,032{\pm}90{\sim}3,332{\pm}63$ at 60 DAT, $2,709{\pm}50{\sim}3,158{\pm}155$ at 75 DAT, $2,535{\pm}49{\sim}2,907{\pm}83$ at 90 DAT, and $2,242{\pm}48mg\;L^{-1}$ at 105 DAT. In addition, appropriate $NO_3{^-}$ content of soil solution was $167{\pm}9{\sim}212{\pm}15$ at 45 DAT, $83{\pm}10{\sim}112{\pm}12$ at 60 DAT, $49{\pm}3{\sim}92{\pm}6$ at 75 DAT, $71{\pm}9{\sim}103{\pm}9$ at 90 DAT, and $73{\pm}9mg\;L^{-1}$ at 105 DAT. The cucumber yield at 100% N level of fertigation was $7,770kg\;10a^{-1}$ and no difference in yield was found at 200% N level of fertigation. However, there was 12% decrease in yield at 50% N fertigation and, 17% decrease at 0% N fertigation. Under retarding culture, $NO_3{^-}$ concentration of leaf petiole juice was highest at 55 days after transplanting (DAT) at all fertigation treatments. Appropriate $NO_3{^-}$ content of leaf petiole juice was $2,464{\pm}102{\sim}2,651{\pm}33$ at 45 DAT, $3,025{\pm}71{\sim}3,314{\pm}84$ at 55 DAT and $2,488{\pm}92mg\;L^{-1}$ at 65 DAT, respectively. Appropriate $NO_3{^-}$ content of soil solution was $111{\pm}10{\sim}155{\pm}14$ at 45 DAT, $93{\pm}7{\sim}147{\pm}14$ at 55 DAT, $67{\pm}4mg\;L^{-1}$at 65 DAT, respectively. The cucumber yield at 50% N fertigation was not different from $1,697kg\;10a^{-1}$ of 100% N fertigation level and even with that of the 200% N fertigation. However, there was 21% decrease in yield at 0% N fertigation.