• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1192-1195
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• 2010

To investigate the influence of the N- or C-terminal regions of pleurocidin (Ple) peptide on antifungal activity, four analogs partially truncated in the N- or C-terminal regions were designed and synthesized. Circular dichroism (CD) spectroscopy demonstrated that all the analogs maintained an alpha-helical structure. The antifungal susceptibility testing also showed that the analogs exhibited antifungal activities against human fungal pathogens, without hemolytic effects against human erythrocytes. The result further indicated that the analogs had discrepant antifungal activities [Ple>Ple (1-22)>Ple (4-25)>Ple (1- 19)>Ple (7-25)] and that N-terminal deletion affected the activities much more than C-terminal deletion. Hydrophobicity [Ple>Ple (1-22)>Ple (4-25)>Ple (1-19)> Ple (7-25)] was thought to have been one of the consistent factors that influenced these activity patterns, rather than the other primary factors like the helicity [Ple>Ple (4-25) >Ple (1-22)>Ple (1-19)>Ple (7-25)] or the net charge [Ple=Ple (4-25)=Ple (7-25)>Ple (1-22)=Ple (1-19)] of the peptides. In conclusion, the hydrophobic amino acids in the N-terminal region of Ple is more crucial for antifungal activity than those in the C-terminal region.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1386-1394
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• 2013

In our previous study, the 25-mer antimicrobial peptide pleurocidin (Ple) had been thought to induce apoptosis in Candida albicans. This study demonstrated that reactive oxygen species (ROS) production was a major cause of Ple-induced apoptosis. Four truncated analogs were synthesized to understand the functional roles in the N- and C-terminal regions of Ple on the apoptosis. Ple, Ple (4-25), Ple (1-22), and Ple (1-19) produced ROS, including hydroxyl radicals, on the order of [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19)], whereas Ple (7-25) did not induce any ROS production. The results suggested that the N-terminal deletion affected the ROS-inducing activities much more than that of the C-terminal deletion, and net hydrophobicity [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)] was related to ROS generation rather than other primary factors like net charge. Hence, we focused on the N-terminal-truncated peptides, Ple (4-25) and Ple (7-25), and examined other apoptotic features, including mitochondrial membrane depolarization, caspase activation, phosphatidylserine externalization, and DNA and nuclear fragmentation. The results also confirmed the disappearance of apoptotic activity of Ple (7-25) by the truncation of the N-terminal region (1-6) and the specific activity patterns between Ple and analogs. In conclusion, the N-terminal region of Ple played an important role in apoptosis.

• The KIPS Transactions:PartD
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• v.13D no.1 s.104
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• pp.51-60
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• 2006

Product Line Engineering(PLE) is an effective software development technique which produces applications using core assets. Because of reusing the core assets, PLE can save cost for developing products in a domain but increase reusability. There are about ten PLE methodologies available, but there are not yet common agreements on PLE process and artifacts. This makes developers harder to choose a methodology and to apply it in practice. A comprehensive technical evaluation and comparison on existing PLE methodologies would be essential for practitioners. In this paper, we present a technical assessment of representative PLE methodologies; FAST, SEI SPL, PuLSE, Bosch's PL proceis, FOPLE, ESAPS, KobrA/PoLITe, Alexandria, COPA, QADA. They are compared in the criteria of process, artifacts, instructions, and special features. And we identify common or variable elements between methodologies and confirm elements to be improved in each PLE methodology. The assessment result would be well utilized in defining a practical methodology for PLE projects and in choosing an appropriate methodology among available ones.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.1
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• pp.52-60
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• 2016

The biological activity of polysaccharide is greatly influenced by polysaccharide structure and molecular distribution. Here, we developed a rapid and convenient isolation method for fractionating polysaccharides with different characteristics and optimized it using a polysaccharide mixture from Korean persimmon leaves. A crude polysaccharide mixture, persimmon leaves-enzyme (PLE) fraction, was isolated from persimmon leaves digested with pectinase and ethanol precipitation. The PLE fraction was further fractionated with a serially diluted ethanol solution (ethanol : deionized water=4:1, 2:1, 1.5:1, 1:1, and 0.5:1) to produce 10 subfractions (five precipitate fractions labeled from PLE-4 to PLE-0.5 and five supernatant fractions labeled from PLE-4S to PLE-0.5S). HPLC analysis indicated that PLE-4 and -2 consisted of diverse polysaccharides, whereas PLE-1.5, -1, and -0.5 contained high molecular weight (MW) polysaccharides. The fractions from PLE-4 to PLE-1 were mostly composed of 13 different characteristic sugars in rhamnogalacturonan (RG) I and II, and the sugars contained an arabino-${\beta}$-3,6-galactan moiety. However, PLE-0.5 did not contain RG-II or ${\beta}$-arabino-3,6-galactan. Treatment of macrophages with fractions PLE-1.5S and PLE-1S led to a $10{\mu}g/mL$ increase in interleukin (IL)-6 production, whereas treatment with PLE-4S and PLE-2S fractions composed of low MW polysaccharides resulted in reduced levels of IL-6. These results indicate that this isolation method may be useful for the rapid and convenient fractionation of bioactive RGs from polysaccharide mixtures with various properties.

• Journal of KIISE:Software and Applications
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• v.32 no.4
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• pp.237-246
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• 2005

Product Line Engineering(PLE) is one of the technologies that develop applications economically reusing core assets. PLE consists of Framework Engineering(FE) and Application Engineering. Framework Engineering is to develop core assets that have common functionality shared by a set of family members. Application Engineering is to develop a specific application by instantiating the core assets. The PLE process increases reusability and efficiency because a specific application is developed by using core assets with less time and effort. Since definition of PLE artifacts and relationship between artifacts are not clear. developers have several troubles to make artifacts based on PLE process, are difficult to maintain consistency between artifacts, and do not use PLE process more practically. In this paper, we define meta-models of artifacts that are produced in PLE activities of PLE process and describe the traceability relationship between artifacts by using traceability map and guidelines that can apply traceability relationship. Finally, we define the way how trace links and guidelines of traceability map are applied.

• Nutritional Sciences
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• v.6 no.3
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• pp.141-147
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• 2003

Fruits and vegetables reportedly have a protective effect against hyperlipidemia and oxidative disease. Accordingly, this study aimed to investigate the lipid-lowering effect and antioxidative capacity of persimmon leaf extract (PLE) in rats fed a high-cholesterol diet. Male rats were fed a high-cholesterol (1% wt/wt) or high-cholesterol diet supplemented with Lovastatin (0.02% wt/wt) or PLE (0.2% wt/wt) for 5 weeks. The concentration of plasma total cholesterol was significantly lower in the PLE group than in the lovastatin group. However, the concentration of plasma HDL-cholesterol and the ratio of HDL-cholesterol/total-cholesterol (%) were significantly higher in the PLE group than in the control group. The PLE supplement also significantly lowered the contents of hepatic cholesterol and triglyceride. In comparing fecal sterol contents, the PLE group saw a significant increase of both neutral and acidic sterol compared to the other groups. The PLE supplement significantly lowered plasma GOT and GPT activity, which ave indices of hepatic toxicity. Plasma TBARS concentration was significantly lower in the PLE group than in the control group, while hepatic TBARS level was not significantly different between the groups. In a comparison of hepatic antioxidant parameters, SOD, catalase and GSH-Px activity were significantly higher in the PLE group than in the control group. However, the PLE supplement significantly towered antioxidant enzyme activity in the erythrocyte. Furthermore, these results suggest that supplementation of PLE promoted the excretion of fecal sterols, thereby leading to decreased absorption of dietary cholesterol. In addition, PLE may play an important role in regulating antioxidative capacities by altering SOD and ChT activity.

• Journal of KIISE:Software and Applications
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• v.32 no.9
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• pp.835-846
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• 2005

A Product Line (PL) is a set of products (applications) that share common assets in a domain. Product Line Engineering (PLE) is a set of principles, techniques, mechanisms, and processes that enables the instantiation of produce lines. Core assets, the common assets, are created and instantiated to make products in PLE. Model Driven Architecture (MDA) is a new software development paradigm that emphasizes its feasibility with automatically developing product. Therefore, we can get advantages of both of the two paradigms, PLE and MDA, if core assets are represented as PIM in MDA with predefined automatic mechanism. PLE framework in the PIM level has to be interpreted by MDA tools. However, we do not have a standard UML profile for representing core assets. The research about representing PLE framework is not enough to make automatically core assets and products. We represent core asset in PIM level in terms of structural view and semantic view. We also suggest a method for representing architecture, component, workflow, algorithm, and decision model. The method of representing framework with PLE and MDA is used to improve productivity, applicability, maintainability and qualify of product.

• Applied Chemistry for Engineering
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• v.30 no.2
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• pp.254-259
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• 2019

In this study, we synthesized the pine leaf extract intercalated layered Mg-phyllosilicate nanoparticles (PLE/MgP) via one-pot synthesis. MgP was successfully synthesized with the octahedral and tetrahedral structure by XRD analysis and a gap of interlayer distance (d-spacing) between MgP sheets by the intercalation of PLE was confirmed. As a result of the investigation of antimicrobial activity against cutaneous microorganisms by the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) analyses, the antimicrobial activity of PLE/MgP was more improved than that of MgP or PLE. The prepared sandwich-structured PLE/MgP organic/inorganic hybrid materials will be useful in the field of numerous applications containing cosmetic and biomedical materials.

• Toxicological Research
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• v.20 no.1
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• pp.37-42
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• 2004

This study was undertaken to investigate comparative anti-tumor activity of water extracts of Phellinus gilvus (PGE), Phellinus linteus (PLE), and Phellinus baumii (PBE) in vitro. The anti-tumor activity in the present study was evaluated by sulforhodamine B (SRB) and microtetrazolium (MTT) assay in terms of cell survival level. The tumor cells (sarcoma 180 and P388) were treated with PGE, PLE, and PBE (7.5, 15, and 30 $\mu\textrm{g}$/ml) and Doxorubicin (DOX) (0.001~10 $\mu\textrm{M}$). The results showed that DOX, PGE, and PLE inhibited proliferation showing a dose-dependent manner against both tumor cells. However, PBE was inhibited by the only 30 $\mu\textrm{g}$/ml in both cells proliferation. In conclusion, all of PGE, PLE, and PBE used in this study have shown anti-tumor activity against both sarcoma 180 and P388. Among them, PLE was the most effective in anti-tumor activity against sarcoma 180 (p<0.05) and PGE was against P388 in SRB assay. PLE, however, was against P388 (p<0.05) in MTT assay.

• Preventive Nutrition and Food Science
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• v.17 no.1
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• pp.14-21
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• 2012

The purpose of this study was to investigate the anti-allergy activities of persimmon leaf extract (PLE) on a phthalic anhydride (PA)-induced allergic mouse model. A human leukemic mast cell line (HMC-1) was used to examine the inhibitory activity of PLE on the histamine release by human leukemic mast cells. PLE inhibited histamine release from HMC-1 cells in response to cross-linkage of high-affinity IgE receptor-${\alpha}$ ($Fc{\varepsilon}RI{\alpha}$). Additionally, a PA-induced allergic mouse model was used to investigate the effects of PLE in vivo. Mice were orally administrated with or without PLE of single dose (250 mg/kg/day) for 31 days. Oral intake of PLE significantly inhibited passive cutaneous reactions. Oral administration of PLE to PA-induced allergic mice also led to a striking suppression of the development of contact dermatitis, ear swelling and lymph node weight. In addition, PA-specific IL-4 production of draining lymph node cells was markedly diminished by PLE oral administration, but not IFN-${\gamma}$. Furthermore, PLE treatment suppressed PA-induced thymus and activation-regulated chemokine (CCL17) and cutaneous T cell-attracting chemokine (CCL27) expressions in ear tissues. Based on these results, we suggest that PLE may have therapeutic potential as an effective material for management of irritant contact dermatitis or related inflammatory diseases.