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Regulation of Phospholipase D by CoCl2 in Human Glioblastoma Cells (인간 교세포주에서 CoCl2에 의한 phospholipase D의 조절기전)

  • Lee, Seung-Hoon;Min, Gye-Sik;Min, Do-Sik
    • Journal of Life Science
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    • v.16 no.4
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    • pp.691-698
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    • 2006
  • Phospholipase D (PLD) is known to play an important role in a variety of cells. However, little is known about $CoCl_2-mediated$ PLD signaling. In this study we demonstrated for the first time that $CoCl_2$ stimulates PLD activity and increases expression of cyclooxygenase-2 (COX-2), which is known to mediate inflammatory reaction. $CoCl_2-induced$ PLD activity was assessed by measuring the formation of $[^3H]$ phosphatidylbutanol (PtdBut), the product of PLD-mediated transphosphatidylation, in the presence of 1-butanol. To study mechanism of PLD signaling induced by $CoCl_2$, U87 human glioblastoma cells were stimulated by $CoCl_2$ and regulators of PLD activity induced by $CoCl_2$ were investigated using several inhibitors of signaling proteins. Moreover, PLD activation by $CoCl_2$ increased not only expression of COX-2 protein but also COX-2 promoter activity. In summary, these results suggest that $CoCl_2$ increases expression of COX-2 protein via PLD in human U87 glioblastoma cells.

Power Consumption Prediction Scheme Based on Deep Learning for Powerline Communication Systems (전력선통신 시스템을 위한 딥 러닝 기반 전력량 예측 기법)

  • Lee, Dong Gu;Kim, Soo Hyun;Jung, Ho Chul;Sun, Young Ghyu;Sim, Issac;Hwang, Yu Min;Kim, Jin Young
    • Journal of IKEEE
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    • v.22 no.3
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    • pp.822-828
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    • 2018
  • Recently, energy issues such as massive blackout due to increase in power consumption have been emerged, and it is necessary to improve the accuracy of prediction of power consumption as a solution for these problems. In this study, we investigate the difference between the actual power consumption and the predicted power consumption through the deep learning- based power consumption forecasting experiment, and the possibility of adjusting the power reserve ratio. In this paper, the prediction of the power consumption based on the deep learning can be used as a basis to reduce the power reserve ratio so as not to excessively produce extra power. The deep learning method used in this paper uses a learning model of long-short-term-memory (LSTM) structure that processes time series data. In the computer simulation, the generated power consumption data was learned, and the power consumption was predicted based on the learned model. We calculate the error between the actual and predicted power consumption amount, resulting in an error rate of 21.37%. Considering the recent power reserve ratio of 45.9%, it is possible to reduce the reserve ratio by 20% when applying the power consumption prediction algorithm proposed in this study.

Development of a flower support for real flower decoration Automatic Production System (생화 장식 꽃받침 자동 생산 시스템 개발)

  • Song, Myung-Seok;Kim, Man-Joong;Kim, Seon-Bong;Ji, Peng;Ryuh, Beom-Sahng
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.7
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    • pp.63-71
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    • 2018
  • A flower support was developed for real flower decoration automation production system using an ultrasonic wave sealer to automatically produce a system. Because a flower support for real flower decoration that was produced manually could not meet the needs of the consumers, this study developed an automated manufacturing system to increase productivity. A flower support for real flower decoration was constructed using a cap consisting of plastic and plate made from non-woven fabric. The guide was designed to transport the cap to the ultrasonic wave sealer and optimal guide was developed from the test according to the material and shape. To produce the entire system, the guides and accessories were weighed and appropriate motors and pulleys were calculated. Control of the automation production system was based on a PCB board, which increased the reliability and security, and a remote controller with manual and automatic modes was prepared. After development, tests of the transfer precision and repetition accuracy revealed an X-axis of 2.7mm, a Y-axis of 1 mm, and a repetition of 0 mm. The productivity was also checked. The automated machine worked 8 hours/day to make 35 supports and 70 Therefore, the automatic system produces 200% more output than manual work

Cudrania Tricuspidata root extract (CTE) has an anti-platelet effect via cGMP-dependent VASP phosphorylation in human platelets (꾸지뽕나무 뿌리 추출물의 cGMP에 의한 VASP 인산화 기전을 통한 항혈소판 효과)

  • Ro, Ju-Ye;Cho, Hyun-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.298-305
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    • 2019
  • Cudrania tricuspidata has been reported to have many biological activities, including anti-inflammatory, anti-cancer, and antioxidant properties. However, the effects of C. tricuspidata root extract (CTE) on human platelet aggregation induced by collagen as well as the signaling pathways involved remain unknown. In the present study, we investigated the effect of CTE on human platelets. CTE inhibited platelet aggregation via down-regulation of thromboxane A2 (TXA2) by blocking cyclooxygenase-1 (COX-1) activity and intracellular Ca2+ mobilization in collagen-induced platelets. CTE also reduced the phosphorylation of phospholipase C (PLC) γ2 and syk. CTE regulated platelet aggregation via cyclic guanosine monophosphate (cGMP)-dependent phosphorylation of vasodilator-stimulated phosphoprotein (VASP) Ser239. In addition, administration of CTE (50 and 100 mg/kg) significantly reduced hyper-aggregated platelet aggregation by collagen (5 ㎍/mL) without hepatotoxicity in HFD (high fat diet)-fed rats. Taken together, these results suggest that CTE has anti-platelet effects both in vitro and in vivo. Therefore, CTE may be an effective therapeutic and preventive agent for cardiovascular disease, and is a safe and natural product.

Independent I/O Relay Class Design Using Modbus Protocol for Embedded Systems

  • Kim, Ki-Su;Lee, Jong-Chan
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.6
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    • pp.1-8
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    • 2020
  • Communication between system modules is applied using the Modbus protocol in industrial sites including smart factories, industrial drones, building energy management systems, PLCs, ships, trains, and airplanes. The existing Modbus was used for serial communication, but the recent Modbus protocol is used for TCP/IP communication.The Modbus protocol supports RTU, TCP and ASCII, and implements and uses protocols in embedded systems. However, the transmission I/O devices for RTU, TCP, and ASCII-based protocols may differ. For example, RTU and ASCII communications transmit on a serial-based communication protocol, but in some cases, Ethernet TCP/IP transmission is required. In particular, since the C language (object-oriented) is used in embedded systems, the complexity of source code related to I/O registers increases. In this study, we designed software that can logically separate I/O functions from embedded devices, and designed the execution logic of each instance requiring I/O processing through a delegate class instance with Modbus RTU, TCP, and ASCII protocol generation. We designed and experimented with software that can separate communication I/O processing and logical execution logic for each instance.

Physiological Roles of Phospholipase Cγ and Its Mutations in Human Disease (Phospholipase Cγ의 생리적 기능과 질병과 연관된 돌연변이)

  • Jang, Hyun-Jun;Choi, Jang Hyun;Chang, Jong-Soo
    • Journal of Life Science
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    • v.30 no.9
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    • pp.826-833
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    • 2020
  • Phospholipase C gamma (PLCγ) has critical roles in receptor tyrosine kinase- and non-receptor tyrosine kinase-mediated cellular signaling relating to the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to produce inositol 1,4,5 trisphosphate (IP3) and diacylglycerol (DAG), which promote protein kinase C (PKC) and Ca2+ signaling to their downstream cellular targets. PLCγ has two isozymes called PLCγ1 and PLCγ2, which control cell growth and differentiation. In addition to catalytically active X- and Y-domains, both isotypes contain two Src homology 2 (SH2) domains and an SH3 domain for protein-protein interaction when the cells are activated by ligand stimulation. PLCγ also contains two pleckstrin homology (PH) domains for membrane-associated phosphoinositide binding and protein-protein interactions. While PLCγ1 is widely expressed and appears to regulate intracellular signaling in many tissues, PLCγ2 expression is restricted to cells of hematopoietic systems and seems to play a role in the regulation of immune response. A distinct mechanism for PLCγ activation is linked to an increase in phosphorylation of specific tyrosine residue, Y783. Recent studies have demonstrated that PLCγ mutations are closely related to cancer, immune disease, and brain disorders. Our review focused on the physiological roles of PLCγ by means of its structure and enzyme activity and the pathological functions of PLCγ via mutational analysis obtained from various human diseases and PLCγ knockout mice.

Effect of substrate bias voltage on a-C:H film (기판 bias 전압이 a-C:H 박막의 특성에 미치는 영향)

  • 유영조;김효근;장홍규;오재석;김근식
    • Journal of the Korean Vacuum Society
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    • v.6 no.4
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    • pp.348-353
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    • 1997
  • Hydrogenated amorphous carbon(a-C:H) films were deposited on p-type Si(100) by DC saddle-field plasma enhanced CVD to investigate the effect of substrate bias on optical properties and structural changes. They were deposited using pure methane gas at a wide range of substrate bias at room temperature and 90 mtorr. The substrate bias voltage ($V_s$) was employed from $V_s=0 V$ to $V_s=400 V$. The information of optical properties was investigated by photoluminescence and transmitance. Chemical bondings of a-C:H have been explored from FT-IR and Raman spectroscopy. The thickness and relative hydrogen content of the films were measured by Rutherford backscattering spectroscopy (RBS) and elastic recoil detection (ERD) technigue. The growth rate of a-C:H film was decreased with the increase of $V_s$, but the hydrogen content of the film was increased with the increase of $V_s$. The a-C:H films deposited at the lowest $V_s$ contain the smallest amount of hydrogen with most of C-H bonds in the of $CH_2$ configuration, whereas the films produced at higher $V_s$ reveal dominant the $CH_3$ bonding structure. The emission of white photoluminescence from the films were observed even with naked eyes at room temperature and the PL intensity of the film has the maximum value at $V_s$=200 V. With $V_s$ lower than 200 V, the PL intensity of the film increased with V, but for V, higher than 200 V, the PL intensity decreased with the increase of $V_s$. The peak energy of the PL spectra slightly shifted to the higher energy with the increase of $V_s$. The optical bandgap of the film, determined by optical transmittance, was increased from 1.5 eV at $V_s$=0V to 2.3 eV at $V_s$=400 V. But there were no obvious relations between the PL peak and the optical gap which were measured by Tauc process.

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Development of Automatic Loading Equipment of Seedling Tray for Automatic Process of Raising Seedlings in Plant Factory (식물공장 육묘공정 자동화를 위한 육묘트레이 자동적재장치 개발)

  • Park, Sang-Min;Min, Young-Bong;Lee, Gong-In;Kim, Dong-Ouk;Kang, Dong-Hyun;Moon, Sung-Dong
    • Journal of agriculture & life science
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    • v.45 no.5
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    • pp.105-113
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    • 2011
  • This study was carried out to develop an automatic loading equipment that can load seedling trays on loading position of the seedling bed driving on enclosed-type rail installed for interconnecting each process of raising seedlings in plant factory. The experiment of transferring the seedling tray by monorail pusher was carried out to figure out the required transfer force and problems during push type device of transporting the plastic seedling trays, that has completed its sowing process, which are installed onto the board of different materials. From the results of this experiment, the loading equipment which can exactly load three of the seedling trays orderly on the loading position of the seedling bed was designed and made. When three sowed trays on every board are transferred by pusher with the speed is at 30 cm/s, the maximum peak transfer force with maximum overshooting at initial transient state and the maximum transfer force at stead state are were respectively 32.8 N, 29.4 N on rubber board, 29.7 N, 22.5 N on a wooden board, 26.9 N, 19.6 N on a acrylic board, and 27.6 N, 19.1 N on an iron board. Changes in the transfer force occurred its maximum at the moment when the pusher collided with the tray, after the collision gradually decreased until it became stable. When two or three trays placed it in order of widthwise are transferred, it is occurred the overlapping of the tray's external bracket. The developed automatic loading equipment with PLC controller did not make any operation error through 100 times of tests, its maximum seedling tray loading speed was 2 sec/tray and its maximum error of transferred location of the tray was 0.5 cm.

A Natural L-Arginine Analog, L-Canavanine-Induced Apoptosis is Suppressed by Protein Tyrosine Kinase p56lck in Human Acute Leukemia Jurkat T Cells (인체 급성백혈병 Jurkat T 세포에 있어서 L-canavanine에 의해 유도되는 세포자살기전에 미치는 단백질 티로신 키나아제 p56lck의 저해 효과)

  • Park, Hae-Sun;Jun, Do-Youn;Woo, Hyun-Ju;Rue, Seok-Woo;Kim, Sang-Kook;Kim, Kyung-Min;Park, Wan;Moon, Byung-Jo;Kim, Young-Ho
    • Journal of Life Science
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    • v.19 no.11
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    • pp.1529-1537
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    • 2009
  • To elucidate further the antitumor effects of a natural L-arginine analogue, L-canavanine, the mechanism underlying apoptogenic activity of L-canavanine and its modulation by protein tyrosine kinase $p56^{lck}$ was investigated in human Jurkat T cells. When the cells were treated with 1.25 to 2.5 mM L-canavanine for 36 h, several apoptotic events including mitochondrial membrane potential (${\Delta\Psi}m$) loss, activation of caspase-9, -3, -8, and -7, poly (ADP-ribose) polymerase (PARP) degradation, and DNA fragmentation were induced without alteration in the levels of Fas or FasL. These apoptotic changes were more significant in $p56^{lck}$-deficient Jurkat clone JCaM1.6 than in $p56^{lck}$-positive Jurkat clone E6.1. The L-canavanine-induced apoptosis observed in $p56^{lck}$-deficient JCaM1.6 cells was significantly reduced by introducing $p56^{lck}$ gene into JCaM1.6 cells by stable transfection. Treatment of JCaM1.6/lck cells with L-canavanine caused a transient 1.6-fold increase in the kinase activity of $p56^{lck}$. Both FADD-positive wild-type Jurkat T cell clone A3 and FADD-deficient Jurkat T cell clone I2.1 exhibited a similar susceptibility to the cytotoxicity of L-canavanine, excluding involvement of Fas/FasL system in triggering L-canavanine-induced apoptosis. The L-canavanine-induced apoptotic sub-$G_1$ peak and activation of caspase-3, -8, and -7 were abrogated by pan-caspase inhibitor (z-VAD-fmk), whereas L-canavanine-induced activation of caspase-9 was not affected. These results demonstrated that L-canavanine caused apoptosis of Jurkat T cells via the loss of ${\Delta\Psi}m$, and the activation of caspase-9, -3, -8, and -7, leading to PARP degradation, and that the $p56^{lck}$ kinase attenuated the ${\Delta\Psi}m$ loss and activation of caspases, and thus contributed as a negative regulator to L-canavanine-induced apoptosis.