• Development and Reproduction
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• v.2 no.1
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• pp.9-20
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• 1998

It has been wel known that phospholipase C(PLC) plays an important role in the intracellular signaling in a variety of cell types. However, involvement of PLC in mouse oocyte maturation and preimplantation embryo development remains unknown. The present study examined the expression patterns of the mouse PLC \beta 1 and \gamma 1 during oocyte maturatio and preimplantation embryo development study examined the expression patterns of the mouse PLC \beta 1 and \gamma 1 during oocyte maturation and preimplantation embryo development by the competitive reverse transcription-polymerase chain reaction (RT-PCR method). PLC \gamma 1 mRNA (0.1 fg) was readily detected in germinal vesicle (GV)-stage oocyte and its level was reduced as meiotic resumption proceeded. PLC-\beta 1 mRNA (<0.1 fg) as detected at low level at GV-stage oocytes and scarcely detected at germinal vescle breakdown (GVBD)-stage oocytes. After fertilization, both PLC \beta 1 and \gamma 1 mRNA levels began to increase at morula-stage embryos (0.2 fg) and were more prominent in blastocyst-stage embryos(1 fg). to elucidate the possible involvement of PLC via protein kinase C(PKC) pathway during oocyte maturation and preimplantation embryo development , the effects of sphingosine (PKC inhibitor), sn-$diC_{8}$(PKC activator) anc U73122 (PLC ingibitor) were examined. Treatment of GV-stage oocytes with sphingosine (20 \mu M) facilitated the meiotic resuption by 10-20 over the control within 1 h as judged by GVBD, whereas U73122 failed to show any significant effect. U73122 (10 \mu M) effectively blocked the compaction of morula, while sn-$diC_{8}$(50 \mu M). In summary, the present study shows that the mouse PLC \beta 1 and \gamma 1 are expressed in a developmental stage-specific manner and PLC-PKC pathway may be involved in early preimplantation embryo development.

• The Transactions of the Korea Information Processing Society
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• v.3 no.2
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• pp.283-294
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• 1996

To operate the automatic devices of manufacturing process more effectively and to solve the needs of the resource sharing, network technology is applied to the unit control devices located in common manufacturing zone and operated by connecting them. This paper introduces deign and test operation of cell system for the management of multivendor PLC by applying to wall paper process based on the manufacturing standardization of CIM. The analysis of designed multivendor PLC group is performed by selecting the parameters such as variation of data packet size and node number of PLC ladder logic program and variation by analyzing the corresponding variation values of token rotation time and waiting time for the system operation. For the method of the analysis,the equation is considering the overhead such as indicated packet service time and transmission safety margin for PLC network connection in M/G/1 queue model,and it is applied to the cell system and PLC group manage-ment for operation .Through the experiment,it is found that the realtime processing is possible with expanded and better result than the resuit obtained by Jayasumana.Browaka with PLC scan time considered,response lower limit of 10-20 msec. data packet size not more than 50 bytes.and the number of nodes less than 40.

• BMB Reports
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• v.28 no.5
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• pp.387-391
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• 1995

In order to investigate whether phospholipase C (PLC) activity in oat celIs is regulated by Gprotein, we have characterized PLC in plasma membranes of oat tissues. To identify the purified plasma membrane, $K^+$-stimulated, $Mg^{2+}$-dependent ATPase activity was measured. The activity of ATPase was shown to be proportional to the concentration of membrane protein. To examine the PLC activity regulated by G-protein, we used the inside-out and outside-out plasma membrane mixture isolated from the oat cells. The plasma membrane mixture showed higher PLC activity than the one of the outside-out plasma membrane. This suggests that PLC activity is located at the cytoplasmic surface of plasma membrane. PLC activity in plasma membrane mixture was dependent on $Ca^{2+}$ with maximum activity at 100 ${\mu}m$ $Ca^{2+}$ and it was inhibited by 1 mM EGTA. Using Sep-pak $Accell^{TM}$ Plus QMA chromatography, we found that inositol 1,4,5-trisphosphate ($IP_3$) was produced in the presence of 10 ${\mu}m$ $Ca^{2+}$. The PLC activity in the membrane was enhanced by an activator of G-protein ($GTP{\gamma}S$) and not by an inhibitor ($GDP{\beta}S$). This indicates that a G-protein is involved in the activation of PLC in the plasma membrane of oat cells.

• IEIE Transactions on Smart Processing and Computing
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• v.6 no.1
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• pp.39-46
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• 2017

In this paper, we propose an integer frequency offset estimation and compensation method based on PLC preamble correlation in DOCSIS 3.1 Downstream system. The proposed method determines the PLC preamble subcarrier location recovered from PLC data and the one obtained from PLC preamble correlation. We showed the performance of PLC preamble detection in the received signal through the maximum value detection of PLC preamble correlation. Thus we can estimate and compensate for the integer frequency offset by computing the difference of PLC subcarrier locations.

• Proceedings of the KIEE Conference
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• 2001.11c
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• pp.65-67
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• 2001

PLC (Power Line Communication) is the communication method using the existing power line installed in houses and offices to convert and transmit high frequency communication signal from tens of KHz to tens of MHz, and receive the filtered signal using high frequency filter. The advantage of PLC is that PLC uses the existing power line installed in houses and offices so it does not require separate power line. Easy and convenient access using electric outlets is another advantage of PLC. However, PLC has some disadvantages such as limited transmission power, high load interference and noise, variable signal attenuation, characteristic of impedance selective possibility of frequency property. This study designed the boiler temperature control system by unit using the modem designed on the basis of technology used for PLC modem, and designed remote control system using internet. After conducting experiments with those two systems, it was possible to control stably. By commercializing this product, we can avoid unnecessary heating of separate temperature control unit, and save the cost according1y, and it is possible to control on a remote site using internet in a more convenient way.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.7
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• pp.741-749
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• 2014

This paper proposes a machine vision module for PLCs (Programmable Logic Controllers). PLC is the industrial controller most widely used in factory automation system. However most of the machine vision systems are based on PC (Personal Computer). The machine vision system embedded in PLC is required to reduce the cost and improve the convenience of implementation. In this paper, we newly propose a machine vision module based on PLC. The image processing libraries are implemented and integrated with the PLC programming tool. In order to interface the libraries with ladder programming, the ladder instruction set was also designed for each vision library. By use of the developed system, PLC users can implement vision systems easily by ladder programming. The developed system was applied to sample inspection system to verify the performance. The experimental results show that the proposed system can reduce the cost of installing as well as increase the ease-of-implementation.

• Journal of the Society of Naval Architects of Korea
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• v.47 no.1
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• pp.93-98
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• 2010

In this paper, PLC which can be installed easily and is stable to transfer datum, is tested for approving its application in a real ship. Internet access service, CCTV monitoring, light control and huge sensor communications are needed for enhancing the convenience and safety of passengers and crew in ships. In order to apply PLC in ships, we surveyed some noises interrupting PLC in ships and investigated the PLC characteristics. The Hannara, a training ship of Korea Maritime University, was used for the test. We measured and analyzed blocking noises using by NI's SCXI-1001. When noises of specific frequency band occurred near the PLC frequency band, PLC transfer capacity was low for mass datum. We developed and verified some methods to apply PLC in a ship under lots of noises.

• Biomolecules & Therapeutics
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• v.1 no.1
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• pp.121-124
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• 1993

To elucidate the effect of antipsychotics and antidepressants on phosphoinositide(Pl) second massenger system, we studied the dose-dependent inhibition of the phosphoinositide-specific phospholipase C(PLC) isozymes, ${\beta}_1,\;{\gamma}_1$ and${\delta}_1,$ by fluphenazine and haloperidol as antipsychotics, and amitriptyline, maprotiline and mianserin as antidepressants. All the antipsychotics and antidepressants tested showed inhibition on at least one of the PLC isozymes with $IC_{50}$ at the concentration between 25 and $250 {\mu}M.$ Maprotiline, mianserin and amitriptyline inhibited 80 to 90% of the activities of all three PLC isozymes at the concentration of $250{\mu}M,$ while haloperidol and fluphenazine inhibited PLC ${\beta}_1$ and${\gamma}_1$ But baclofen didn't inhibit any PLC isozyme. These results suggested that PLC isozymes are inhibited by antipsychotics and antidepessants even though the concentration is high, and these drugs may affect PI signal transduction system by direct inhibition of PLC isozymes.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.3
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• pp.105-110
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• 2005

Programmable Logic Controller(PLC) is the most widely utilized among many sorts of existing controller for the design of factory automation control system, and study about a PLC language is peformed actively. In this paper, we proposed the encoding module that we increase input points when increased of the input machine which is going to control it in the FA design that used PLC. Input point is going to be extended with 2-3 points in the state that cannot equip input unit in basic base any more. Then an extension base, a power supply, an extension cable and an output nit must be equipped particularly. Then a cost must be added very much. This paper proposed the encoding module which extended an input in a small quantity, and we confirmed a feasibility through a simulation.

• Journal of Practical Engineering Education
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• v.6 no.1
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• pp.43-49
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• 2014

It is known that the ability and habit to "flow" could be one of the important indices to evaluate one's life. And the flow experience of university students who are in the important time transferring from the adolescence to the initial adult period will give an important effect firstly to study one's major and map out one's career path, finally to spread out the high quality life. This study investigated the university students who are in School of Mechatronic Engineering to experience the flow in the learning of PLC (Programmable Logic Controller) practice. For this, the flow condition was arranged. And in order to experience the flow in the learning of PLC, lecture plan was established, studying environment was changed and level of difficulty was controlled. Then the implications of the flow were explored by a 5-point Likert scale survey performed to 90 students.