• 한국식물병리학회지
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• 제14권3호
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• pp.217-219
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• 1998

Of over 100 accessions of pepper tested, MC 4 and MC 5, PBC631, PI322727, PI358812, PBC066, Kerting, PI322726, PI369994, PI377688, CMS-B, PI322728, and Jatilaba showed similarly high level of resistance to bacterial wilt (Ralstonia solanacearum).

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.112-112
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• 2017

Rice blast caused by Magnaporthe oryzae is one of the most serious diseases that affect the quantity and quality of rice production. The use of resistant rice varieties would be the most effective way to control the rice blast. However R gene incorporation into the rice variety takes time and pathogen could overcome the R gene effects after for a while. For monitoring the rice blast resistance gene distribution in Korean varieties, the four major blast resistance genes against M. oryzae were screened in a number of Korean rice varieties using molecular markers. Of the 120 rice varieties tested, 40 were found to contain the Pi-5 gene, 25 for the Pi-9 gene, 79 for Pi-b and 40 for the Pi-ta gene. None of these rice varieties includes tested 4 R genes. 3 R genes combination, Pi-5/Pi-9/Pi-b, Pi-5, Pi-9.Pi-ta, or Pi-9/Pi-b/Pi-ta were found in 12 varieties, the rice blast disease severity were showed as resistant in the rice verities containing Pi-9/Pi-b/Pi-ta R genes combination, respectively. Also pathogenic diversity of M. oryzae isolates collected in the rice field from 2004 to 2015 in rice field in Korea were analyzed using rice blast monogenic lines, each harboring a single blast resistance gene. Compatibility of blast isolates against rice blast monogenic lines carrying the resistance genes Pi5, Pi9, Pib, and Piz showed dynamic changes by year. It indicates that pathogen has high evolutionary potential adapted host resistances to increase fitness and would lead to rice blast resistance bred into the cultivar becoming ineffective eventually.

• Current Research on Agriculture and Life Sciences
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• 제9권
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• pp.127-133
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• 1991

역병에 저항성인 PI201232와 더뎅이병 저항성인 PI271322와 PI163192를 교배하여 각 병해에 대한 저항성과 두가지 병해 저항성간의 유전적 관계를 검토하였다. 더뎅이병균 race 3에 대한 PI271322의 비과민반응형 저항성은 양적으로 유전하였다. PI201232의 역병에 대한 저항성은 2개의 우성유전자에 가까운 양식으로 유전하였다. PI271322의 더뎅이병균 race 1에 대한 과민반응형 저항성은 한개의 우성유전자 양식으로 유전하였다. PI163192는 더뎅이병균 race에 비특이적으로 저항성이었으며 우성효과가 큰 양적유전 양식으로 유전하였다. 더뎅이병균에 대한 저항성은 역병에 대한 저항성과는 독립적으로 유전하였다.

• The Plant Pathology Journal
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• 제16권2호
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• pp.105-109
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• 2000

The Pi-b is the rice gene conferring race specific resistance to the blast fungus Magnaporthe grisea race having a corresponding avirulence gene, AVR-Pi-b. All resistant cultivars have two copies of the Pi-b gene, but susceptible cultivars have a single copy of the gene. About 1 Kbp insertion sequence was detected in the open reading frame of the Pi-b gene from the susceptible cv. Nipponbare. The nature of insertion sequence was identified as a solo long terminal repeat (LTR) of new rice Tyl-copia-like retrotransposon. LTR was widely distributed in the rice genome. Various types of different patterns of restriction fragment length polymorphism of LTR were detected in indica cultivars, whereas a single type was detected from japonica cultivars. The insertion of LTR sequence in the Pi-b gene in the susceptible cultivar suggested that retrotransposon-mediated insertional mutation might played an important role in the resistance breakdown as well as evolution of resistance genes in rice.

• Molecules and Cells
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• 제42권9호
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• pp.637-645
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• 2019

Effector-triggered immunity (ETI) is an effective layer of plant defense initiated upon recognition of avirulence (Avr) effectors from pathogens by cognate plant disease resistance (R) proteins. In rice, a large number of R genes have been characterized from various cultivars and have greatly contributed to breeding programs to improve resistance against the rice blast pathogen Magnaporthe oryzae. The extreme diversity of R gene repertoires is thought to be a result of co-evolutionary history between rice and its pathogens including M. oryzae. Here we show that Pii is an allele of Pi5 by DNA sequence characterization and complementation analysis. Pii-1 and Pii-2 cDNAs were cloned by reverse transcription polymerase chain reaction from the Pii-carrying cultivar Fujisaka5. The complementation test in susceptible rice cultivar Dongjin demonstrated that the rice blast resistance mediated by Pii, similar to Pi5, requires the presence of two nucleotide-binding leucine-rich repeat genes, Pii-1 and Pii-2. Consistent with our hypothesis that Pi5 and Pii are functionally indistinguishable, the replacement of Pii-1 by Pi5-1 and Pii-2 by Pi5-2, respectively, does not change the level of disease resistance to M. oryzae carrying AVR-Pii. Surprisingly, Exo70F3, required for Pii-mediated resistance, is dispensable for Pi5-mediated resistance. Based on our results, despite similarities observed between Pi5 and Pii, we hypothesize that Pi5 and Pii pairs require partially distinct mechanisms to function.

• 한국작물학회지
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• 제56권4호
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• pp.329-341
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• 2011

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.

• Journal of Information Technology Applications and Management
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• 제14권4호
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• pp.199-219
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• 2007

This research analyze the impact factors that affect the PI resistance of securities brokerage salespersons during Process Innovation in securities companies focusing on the broker's individual personality. After reviewing some related literatures, a survey was conducted at a domestic securities company with the derived factors from a focus group of securities salesmen with over 10 year work experience. The results show that broker's individual propensity to innovation, individual customer relationship and individual flexibility are closely related to the PI resistance. By controlling such factors for salespersons, securities companies can boost the ability to meet and control the changing situation and management innovation.

• Current Research on Agriculture and Life Sciences
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• 제20권
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• pp.83-90
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• 2002

역병 저항성 재료로 도입하여 유지하고 있는 PI123469, PI201234, PI201232, AC2258 (=Line 29), CM334, KC268, KC358, KC820, KC821, KC822, KC823 (Line 29 = AC2258), KC462, KC463, KC464 혹은 이들 유전자원에서 선발한 계통들의 역병에 대한 저항성 검정, 원예적 특성 조사 및 종자증식을 실시하였다. PI123469, PI201234, PI201232, AC2258, KC823에서 선발된 계통들이 가장 저항성이 강한 것으로 나타났다. 동일한 유전자원에서 선발한 계통 간에도 저항성 수준에서 현저한 차이가 관찰되는 경우가 있었으며, 이러한 경우는 주로 유지 증식과정에 자연교잡이 일어난 결과로 추정되었다. 따라서 순도 높은 저항성 재료를 얻기 위해서는 봉지를 씌워 자식종자를 채종하는 것이 가장 안전하며, 다음으로는 계통 망실 혹은 망상을 이용하는 것이 바람직한 것으로 판단되었다.

• Current Research on Agriculture and Life Sciences
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• 제4권
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• pp.7-11
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• 1986

한국(韓國) 지방(地方) 재래종(在來種) 고추 9계통(系統)과 미국(美國) 식물(植物) 도입국계통(導入局系統)(PI) 34개(個) 계통(系統)을 공시(公試)하여 흑색(黑色) 병징(病徵)의 탄저병(炭疽病)(Colletotrichum dematium)에 대한 저항성(抵抗性)을 검정(檢定)하였다. 적열과(赤熱果)에다 침상후(針傷後) 병원균(病原菌) 현탁액(懸濁液)을 분무(噴霧)하는 방법(方法)과 점적(點滴)하는 방법(方法)으로 접종(接種)한후 침상부위(針傷部位)를 중심(中心)으로 형성(形成)된 병반(病班) 직경(直徑)을 측정(側定)하여 저항성(低抗性)을 평가(評價)한 결과(結果)는 다음과 같다. 1) PI 201232, PI 224451, PI 257044, PI 257119, PI 257099, PI 224433, PI 244668, PI 257102, PI 173877, 남지 청룡 서동 등(等)은 발병(發病)이 가장 적어 저항성(抵抗性)으로 사료(思料)된다. 2) PI 241670, PI 244670, PI 224423 등(等)은 발병(發病)이 가장 커 이병성(罹病性)으로 사료(思料)된다. 3) 기외(其外)는 중간(中間) 정도(程度)였다.

• 한국육종학회지
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• 제40권3호
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• pp.215-222
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• 2008

Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.