• Journal of Microbiology and Biotechnology
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• v.6 no.2
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• pp.92-97
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• 1996

Portage kinetic constants of peptide transport can be measured by competitive spectrophotometry. The kinetic constants of L-Glu-L-Glu transport in Escherichia coli were ascertained using L-Phe-L-3-thia-Phe (PSP) as a detector. Since the production of thiophenol upon intracellular hydrolysis of PSP was competitively inhibited by L-Glu-L-Glu, it was able to compute the kinetic constants of L-Glu-L-Glu using this method. The resulted data were in agreement with the values obtained by the method of Michaelis-Menten kinetics. The potential of this method was examined against dipeptide transport systems in various microorganisms. These results strongly suggest that the overall properties of individual systems for dipeptide transports can be easily characterized by competitive spectrophotometry.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.107-110
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• 2001

Among the tested ten enzymes, Optimase M-440 showed the highest activity in transesterification of N-t-Boc-L-Phe-OTFE with D-glucose. Monosaccharides and their derivatives acted as good acyl acceptors in the Optimase M -440 catalyzed transesterification of N-t-Boc-L-Phe-OTFE. Optimase M-440 showed a preferable catalytic activity on the primary hydroxyl group of saccharides and a good regioselectivity. Optimase M-440 showed the highest activity in pyricline among the tested solvents. As acyl donors, trifluoroethyl esters of amino acids showed a high reactivity in transesterification. Optimase M-440 showed a broad substrate specificity towards amin 。 acid esters and saccharides.

• Journal of Advanced Marine Engineering and Technology
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• v.25 no.3
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• pp.581-587
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• 2001

Plate heat exchange(PHE) will be applied to the refrigeration and air conditioning systems as evaporators or condensers for their high efficiency and compactness. The purpose of this study is the analyze the characteristics of heat transfer and pressure drop of plate heat exchanger. Numerical work was conducted using the FLUENT code k-$\varepsilon$model. Also the dependence of heat transfer coefficient and friction factor on Reynolds number was investigated. As the Reynolds number increases, it is found that heat transfer coefficient also increases, but friction factor decreases. The study examines the internal flow, thermal distribution and the pressure distribution in the channel of plate heat exchanger. The results of CFD analysis compared with experimental data, and the difference of friction factor and Nusselt number in plate heat exchanger are 10% and 20%, respectively, Therefore the CFD analysis model is effective for the performance prediction of plate heat exchanger.

• KSBB Journal
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• v.14 no.2
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• pp.235-240
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• 1999

Simple and rapid screening methods were developed to screen mutant strains of Trigonopsis variabilis ATCC10679 (TW). D-amino acid oxidase (D-AAO) from a mutant strain, T26, showed about 30% higher specific activity against cephalosporin C than from its wild type, TW. D-AAO genes from both TW and T26 strains were cloned and sequenced. There was one nucleotide changed from T to C at 811 position, resulting in an amino acid codon changed from Phe-258 to Ser-258.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.15 no.1
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• pp.59-66
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• 2003

The purpose of this study is to analyze the characteristics (COP) of the heat pump system for various operating conditions with the use of seawater heat source and exhaust energy. To accomplish the goal, first of all, the computer simulation for heat pump system is carried out. The heat pump system model is made of a waste heat recovery system and a vapor compression refrigeration system, and the working fluid is R-22. The model calculated the change of COP with the variation of temperature and flow rate. The COP and the plate heat exchanger (PHE) area of the heat pump system are considered moderately high in the condensation temperature of $25^{\circ}^C$ and the evaporation temperature of $2^{\circ}^C$ in indoor culture system. The simulation results will be used effectively for the design and the performance prediction of heat pump system using unused energy in a land base aquaculture system.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.1
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• pp.50.1-50.1
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• 2010

We have analyzed times of minima for of 6 binary systems. Three binary systems show period decrease at rate $3.19{\times}10-5$ yr -1 for SV Cen, $1.35{\times}10-7$ yr -1 for RT Scl and $1.14{\times}10-7$ yr -1 for AD Phe. Two systems show period increase $5.696{\times}10-8$ yr -1 for SX Aur and $6.93{\times}10-8$ yr -1 for GO Cyg. One system shows cyclic period variation. We estimated the mass transfer rate for 5 binary systems. Four systems show asymmetric light curves. Two asymmetric light curves (SV Cen and RT Scl) are due to hot spot caused by mass transfer. And two asymmetric light curves (AD Phe and TY Boo) are due to cool spot caused by magnetic activities on the cooler component. We also obtain absolute dimensions from photometric solution and spectroscopic solution by analyzing their light curves and radial velocity curves, which are collected from literatures, using 2007 version Wilson and Deviney computer code.

• Journal of Microbiology and Biotechnology
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• v.2 no.1
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• pp.1-6
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• 1992

The aspartame, $\alpha$-L-aspartyl-L-phenylalanine methylester, is an artificial sweetener. Taking advantage of the fact that the aspartame is a derivative of dipeptide, synthesis of aspartame from the artificial polypeptide made by an artificial gene has been attempted. The artificial polypeptide (LAP32), a polymer of tripeptide (aspartyl-phenylalanyl-lysine), was purified from the E. coli cells harboring a recombinant plasmid containing the artificial gene. This polypeptide was then digested with trypsin and carboxypeptidase B to produce dipeptide (Asp-Phe). Using the esterase activity of $\alpha$-chymotrypsin, the dipeptide was directly converted into Asp-Phe methylester in a water-methanol system. When the methanol concentration in reaction mixture was 25%, 50% of dipeptide was converted to the dipeptide methylester without producing any by-products.

• Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.15-19
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• 2012

A new cyclic peptide (six-membered amino acid), gamakamide-E (L-Leu-L-Met (SO)-L-Me-Phe-L-Leu-D-Lys-L-Phe), was isolated as a strongly bitter tasting compound from cultured oysters, Crassostrea gigas. The molecular formula of $C_{43}H_{61}N_7O_8S$ was deduced from high resolution fast atom bombardment mass spectrometry (HR FAB-MS) ($[M+H]^+$ m/z 836.4356 ${\Delta}$= -2.4 mmu). Its unique structure including a hydantoin structure was firstly elucidated by nuclear magnetic resonance (NMR) analysis. Stereochemistries of constituent amino acids were determined by chiral high performanced liquid chromatography analysis of natural and synthesized peptides.

• Korean Chemical Engineering Research
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• v.56 no.6
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• pp.792-797
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• 2018

We have explained the synthesis of novel phenanthrene and pyrene substituted phthalocyanines (PC-PHE and PC-PYR) and fully confirmed the structures by its spectral, photo physical and elemental analysis. For these phthalocyanines we checked the UV-Visible absorbance in PGMEA and chloroform and transmittance checked in PGMEA. The transmittance results suggested that these phthalocyanines are showing more than 90% transmittance at the 450-550 nm region. These synthesized molecules are nicely soluble in almost all industrial solvents. We checked the aggregation property of these phthalocyanines in PGMEA, and the results suggested no any aggregation for these molecules in PGMEA. The thermogravimetric analysis results concluded that PC-PHE and PC-PYR had high thermal stability. All studies explain that these new phthalocyanines are more suitable for LCD green color filter application.

• BMB Reports
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• v.44 no.10
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• pp.686-691
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• 2011

Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine, hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore, the mutated product appears to be a feasible therapy for patients with neutropenia.