• Korean Journal of Microbiology
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• v.48 no.3
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• pp.200-206
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• 2012

A bacterial strain capable of synthesizing polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units was isolated from activated sludge using the enrichment culture technique. The organism, identified as Pseudomonas aeruginosa P-5, produced polyesters consisting of 3-hydroxyvalerate and medium-chain-length (MCL) 3-hydroxyalkanoate monomer units when $C_{-odd}$ alkanoic acids such as nonanoic acid and heptanoic acid were fed as the sole carbon source. Solvent fractionation experiments using chloroform and hexane revealed that the 3-hydroxyalkanoate monomer units in these polyesters were copolymerized. The molar concentration of 3-hydroxyvalerate in the polyesters produced were significantly elevated up to 26 mol% by adding 1.0 g/L valeric acid as the cosubstrate. These copolyesters were sticky with low degrees of crystallinity. The PHA synthase genes were cloned, and the deduced amino acid sequences were determined. P. aeruginosa P-5 possessed genes encoding MCL-PHA synthases (PhaC1 and PhaC2) but lacked the short-chain-length PHA synthase gene, suggesting that the MCL-PHA synthases from P. aeruginosa P-5 are uniquely active for polymerizing (R)-3-hydroxyvaleryl-CoA as well as MCL (R)-3-hydroxyacyl-CoAs.

• KSBB Journal
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• v.31 no.1
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• pp.27-32
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• 2016

Several CoA transferases from Clostridium beijerinckii, C. perfringens and Klebsiella pneumoniae were examined for biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) in recombinant Escherichia coli XL1-Blue strain. The CB3819 gene and the CB4543 gene from C. beijerinckii, the pct gene from C. perfringens and the pct gene from K. pneumoniae, which encodes putative CoA transferase gene, respectively, was co-expressed with the Pseudomonas sp. MBEL 6-19 phaC1437 gene encoding engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 ($PhaC1_{Ps6-19}$) to examine its activity for the construction of key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli XL1-Blue expressing the phaC1437 gene and CB3819 gene synthesized poly(3-hydroxybutyrate) [P(3HB)] homopolymer to the P(3HB) content of 60.5 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 3-hydroxybutyrate. Expression of the phaC1437 gene and CB4543 gene in recombinant E. coli XL1-Blue also produced P(3HB) homopolymer to the P(3HB) content of 51.2 wt% in the same culture condition. Expression of the phaC1437 gene and the K. pneumoniae pct gene in recombinant E. coli XL1-Blue could not result in the production of PHAs in the same culture condition. However, the recombinant E. coli XL1-Blue expressing the phaC1437 gene and the C. perfringens gene could produce poly(3-hydroxybutyrate-co-lactate [P(86.4mol%3HB-co-13.7 mol%LA) up to the PHA content of 10.6 wt% in the same culture condition. Newly examined CoA transfereases in this study may be useful for the construction of engineered E. coli strains to produce PHA containing novel monomer such lactate.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.2
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• pp.109-114
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• 2013

Polyhydroxyalkanoates (PHAs) are synthesized by numerous bacteria as carbon and energy storage compounds and are raw materials for biocompatible plastics. In this paper, the effect of $CO_2$ concentrations on the growth of C. necator and the accumulation of Poly(3-hydroxybutyrate) (P(3HB)) are investigated by increasing the $CO_2$ concentration in the substrate gas mixture. During 6 d cultivation in a nitrogen-present mineral medium, the $CO_2$ concentration did not affect the growth of the cells, while the Poly(3- hydroxybutyrate) (P(3HB)) content decreased with increasing $CO_2$ concentrations from 1% to 20%. During 4 d cultivation in the nitrogen-limited medium, the P(3HB) accumulation was the greatest at 3% $CO_2$; however, the total amount of accumulated P(3HB) was the greatest at 1% $CO_2$, which decreased with increasing $CO_2$ concentrations. The results indicate that the gas mixture with 1% $CO_2$ is the most effective in both growing the cells and accumulating P(3HB) under our experimental conditions.

• Journal of Microbiology and Biotechnology
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• v.3 no.2
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• pp.115-122
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• 1993

Poly(3-hydroxybutyrate)(P(3HB)) accumulation under nutrient-rich condition with various amounts of $(NH_4)_2 SO_4$ was systematically investigated. The results of the electron-microscopy and the solvent extraction showed that the P(3HB) accumulation is unavoidable even under nutrient-rich condition. This indicates that in a two-step culture of Alcaligenes eutrophus H16, the researches should be careful in interpreting the data of polyhydroxyalkanoates(PHAs) accumulation in terms of the carbon-source fed in the second step because the two-step culture product contains the P(3HB) produced under nutrient-rich condition. The polyester production capability in a two-step batch culture of A. eutrophus H16(ATCC 17699) was also investigated using various saccharides and their derivatives such as glucose, fructose, gluconic acid, glucaric acid, sorbitol, lactose, galactose, and mannose. The polyesters synthesized were characterized by 500 MHz$^{1}H-NMR$ spectroscopy, intrinsic viscosity$[\eta]$ measurement in chloroform and differential scanning calorimetry(DSC). 500 MHz $^{1}H-NMR$ analysis showed that all polyesters synthesized generally contained 1~2 mol% of 3HV. Another finding is that the glucose utilization can be increased by changing the autoclaving procedure of the substrate to enhance the P(3HB) production yield up to 46 wt% of P(3HB) in dry cells.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.49.3-50
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• 2016

Deep Ecliptic Patrol of the Southern Sky (DEEP-South) observations have been conducted officially during the off-season for exoplanet search since October 2015. Most of the allocated time for DEEP-South is devoted to targeted photometry, Opposition Census (OC), of Near Earth Asteroids (NEAs) to increase the number of such objects with known physical properties. It is efficiently achieved by multiband, time series photometry. This Opposition Census (OC) mode target objects near their opposition, with km-sized PHAs in the early stage and goes down to sub-km objects. Continuous monitoring of the sky with KMTNet is optimized for spin characterization of various kinds of asteroids, including binaries, satellites, slow/fast- and non-principal axis-rotators, and hence is expected to facilitate the debiasing of previously reported lightcurve observations. We present the preliminary lightcurves of NEAs from year one of the DEEP-South with our long term plan.

• Polymer(Korea)
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• v.28 no.1
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• pp.92-102
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• 2004

(6-Cholesteryloxycarbonylpentoxypropyl)celluloses (CHPCs) with degree of esterification (DE) ranging from 2.25 to 2.91 were synthesized by reacting hydroxypropyl cellulose with 6-cholesteryloxycarbonylpentanoyl chloride. The acrylic esters of CHPCs (CHPCEs) and their photocrosslinked films with liquidcrystalline order were also synthesized. The thermotropic properties of mesophase for both uncrosslinked and crosslinked samples and the swelling behavior of the crosslinked samples in acetone were investigated. The hydroxypropyl cellulose exhibited an enantiotropic cholesteric phas, while all the uncrosslinked cholesterylbearing samples exhibited a monotropic cholesteric phases; the 6-cholesteryloxycarbonylpentanoyl chloride also showed a monotropic smectic phase. The hydroxypropyl cellulose formed a right-handed helix whose optical pitch (λ$\sub$m/) increases with temperature, whereas all the uncrosslinked derivatives farmed left-handed helices whose λ$\sub$m/'s decreased with temperature. The thermal stability of the mesophase and the magnitude of λ$\sub$m/ at the same temperature for both CHPCs and CHPCEs decreased with increasing DE. All the crosslinked samples, in constrast with CHPCEs, did not display reflection colors but exhibited an anisotropic swelling characteristic of crosslinked gel retaining liquid-crystalline order.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.64-69
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• 2003

Pseudomonas chlororaphis HS21 was isolated from a soil sample and found to produce medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using palm kernel oil (PKO) as the sole carbon source. Up to 3.3 g/1 dry cell weight containing $45\%$ MCL-PHA was produced, when the strain was grown for 21 h in a jar fermentor culture containing 5 g/1 PKO. The polymer produced from PKO consisted of unsaturated monomers of $7.3\%$ 3-hydroxy-5-cis-tetradecenoate and $2.3\%$ 3-hydroxy-5,8,-cis, cis-tetradecadienoate as well as saturated even-carbon number monomers ranging from $C_6\;to\;C_14$, as determined by GC and El GC/MS The PHA was a transparent, sticky material at room temperature. A differential scanning calorimetric analysis revealed that the polymer was amorphous with a $-44^{\circ}C$ glass transition temperature. The number average molecular weight and polydispersity index of the PHA were 83,000 and 1.53, respectively. Although the PHA was practically biodegradable, its degradability was lower than that of poly(3-hydroxyoctanoate) based on a comp:trison of the clear zones formed by growing PHA depolymerase-producing bacteria on an agar plate containing the respective polymers.

• Journal of the Korea Computer Industry Society
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• v.5 no.1
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• pp.9-18
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• 2004

Successful project planning relies on a good estimation of the manpower required to complete a project in addition to the schedule options that may be available. Existing software manpower estimation models present the total manpower and instantaneous manpower distribution for the software life cycle. Putnam's Rayleigh and Phillai et al.'s Gamma models present a model with assumption that the manpower is needed at the system delivery. This means that 40 percent of total manpower is applied at the software development, and the other 60% is applied during maintenance phase. However Warburton observes the manpower is needed during development phase with the peak at the completion of the software design phase. So, the existing models were not appropriate to be applied to practical projects. This paper suggests the Sigmoid model which does not consider the point of manpower peak to fix the problem above. The suggested model showed some improvement when practical data was applied. Therefore, the Sigmoid model can be used as alternative of Rayleigh and Gamma model to estimate distribution of manpower during software development phas.

• KSBB Journal
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• v.29 no.6
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• pp.399-404
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• 2014

Production of biodegradable polymer polyhydroxyalkanoates (PHAs) from industrial wastes exhibits several advantages such as recycle of waste and the production of high valuable products. To this end, this study aimed at isolating from the sewage treatment plant a PHA producing bacterium capable of utilizing wastes generated from biodiesel and food industries. A Bacillus cereus strain capable of producing poly(3-hydroxybutyrate) [P(3HB)] was isolated, which was followed by confirmation of P(3HB) accumulation by gas-chromatographic analyses. Then, the effects of nutrient limitation on P(3HB) production by B. cereus was first examined. Cells cultured in a minimal medium under the limitation of nitrogen, potassium and sulfur suggested that nitrogen limitation allows the highest P(3HB) accumulation. Next, production of P(3HB) was examined from both waste of biodiesel production (crude glycerol) and waste from food industry (spent coffee grounds). Cells cultured in nitrogen-limited minimal medium supplemented crude glycerol and waste spent coffee grounds extract accumulated P(3HB) to the contents of 2.4% and 1.0% of DCW. This is the first report demonstrating the capability of B. cereus to produce P(3HB) from waste raw materials such as crude glycerol and spent coffee grounds.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.3
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• pp.63-68
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• 2014

This paper related with the I-CMA (Improved-CMA) algorithm that is possible to compensates of phase in CMA adatpve equalizer which is used for the elemination of intersymbol interference in the multipath fading and band limit characteristics of channel. The new cost function is proposed for the eliminate the amplitude and phase simulataneous by modifying the cost fuction for get the error signal in present CMA algorithm. It has a merit to the algorithm simplicities and eliminats the PLL device for phase compensation after equalization. For proving this, the recovered signal constellation that is the output of equalizer output signal and the residual isi and Maximum Distortion charateristic learning curve that are presents the convergence performance in the equalizer and the overall frequency transfer function of channel and equalizer were used. As a result of computer simulation, the I-CMA has more good compensation capability of amplitude and phas in the recovered constellation. But the convergence time is slow due to the simultaneously phase compensation.