• Journal of the Korean Applied Science and Technology
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• v.38 no.1
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• pp.99-106
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• 2021

To circumvent the skin problem from phenylethanol (PhE), we have studied on the enzymatic synthesis of phenylethanol galactoside (PhE-gal) as an alternative to PhE. Base on the previous study, we optimized the reaction conditions for PhE-gal synthesis from PhE using E. coli β-galactosidase (β-gal). The optimal amount of β-gal, PhE concentration, pH, and temperature for PhE-gal synthesis were 0.45 U/ml, 1%, 8.0, 40℃, respectively. Under these conditions, about 81.9 mM PhE was converted into about 47.4 mM PhE-gal, in which the conversion yield was about 57.9%. Meanwhile, when the reaction mixture containing PhE and PhE-gal was mixed and fractionated with water-immiscible solvent (EA or MC), it was observed that PhE-gal was distributed in water phase, and PhE was distributed in solvent phase. Additionally, PhE-gal was clearly distributed into water phase when MC was used, but PE-gal was not when EA was used. In the future, we are planning to carried out the continuing study on developing an alternative cosmetic preservative using PhE-gal.

• The Korean Journal of Zoology
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• v.13 no.4
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• pp.101-111
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• 1970

저자들은 1970년 8월에 채집된 거제도산 지렁이 총 251 개체를 정리한 결과 다음 2과 2속 9종을 얻었다. Pheretima acincta, Ph. agrestis, Ph. geojeinsulae n. sp., Ph. gucheonensis n. sp., Ph. heteropoda, Ph. hilgendorfi, Ph. koreana and Ph. soulensis; Allolobophora caliginosa trapezoides. 거제도의 지렁이에 관해서는 지금까지 보고된 바 없으므로 이들은 전부 거제도 미기록종이되며 이들 중에는 한국 미기리록종 Ph. acincta(Goto et Hatai, 1899)와 두개의 신종, Ph. geojeinsulae, Ph. gucheonensis를 포함하고 있다. Ph. geojeinsulae는 체장 185-210mm, 체폭 10-12mm, 체절수 125-136개, spermathecal pore는 2雙으로서 5/6 및 6/7에 있다. 본 종은 spermathecal pore region과 male pore region의 모양으로 이 속의 다른 종과는 뚜렷이 구별된다. 또 Ph. gucheonensis는 일본산의 Ph. tappensis Ohfuchi, 1935와 비슷하나 체장이 짧으며 (70:100), 특히 내부의 spermatheca의 모양이 전혀 다를 뿐 아니라 10/11, 11/12의 septa가 있는 점으로 뚜렷이 구별된다.

• The Korean Journal of Zoology
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• v.13 no.1
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• pp.9-14
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• 1970

제주도산 육서 빈모류의 분류학적 연구는 Kobayashi, S. (小林新二郞)에 依하여 1937년에 Drawida anchingiana, Pheretima kanrazana, Ph. masatakae, Ph. quelparta 등의 4종이 보고 되었고, 다시 1938년에 Dr. japonica, Ph. carnosa, Ph. hupeiensis가 보고 되었을 뿐이다. 저자들은 1966년 및 1969년에 채집된 제주도산 지렁이 총 1045개체를 정리한 결과 다음 2과 2속 11종을 얻었기에 이에 보고 하는 바이다. 제주도 기지종 : Pheretima carnosa, Ph. hupeiensis, Ph. kanrazana, Ph. masatakae, Ph. quelparta. 제주도 미기록종 : Allolobophora caliginosa trapezoides, Ph. agrestsi, Ph. heteropoda. Ph. phaselus var. kamitai, Ph. soulensis. 신종 : Ph. seungpanesis n. sp. 신종 pheretima seungpanensis의 체장은 $105 \\sim 120mm$, 체폭은 $5 \\sim 6mm$, 체절수는 $78 \\sim 98$개 spermathecal pore는 2쌍으로서 첫째쌍은 VI체절의 setal line 뒷쪽에서 이 선과 인접해 있으며 두째쌍은 setal line의 앞쪽에 intersegmental furrow에 가까운 부분에서 개구한다. 본종은 spermathecal pore의 위치가 이속의 다른 종에서 보는것 처럼 intersegmental furrow에 개구하지 않는점이 특별하다. 그 뿐 아니라 male pore region의 모양에 있어서도 모든 기지종과 뚜렷이 구별된다.

• The Korean Journal of Zoology
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• v.16 no.1
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• pp.5-12
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• 1973

著者들은 1968, 1971 및 1972년의 3個年間 소백산에서 採集한 지렁이 총 1,850個體를 調査 整理한 결과 Pheretima agrestis, Ph. gucheonensis, Ph. hilgendorfi, Ph. jiriensis, Ph. koreana, Ph. soulensis 및 Ph. susakii patina 의 7기지종과 신종 Ph. planta 와 Ph. sopaikensis 등 총 1屬 9種을 얻었다. 신종 Ph. planta는 체장 105mm., 체폭 5.5mm., 체절수 101개이며, spermathecal pore 2雙으로서 vi체절과 vii 체절 腹面 setal line 바로 위에 位置한다. 또 male pore region의 양모에 있어서 뚜렷한 特徵을 나타내고 있다. 신종 Ph. sopaikensis 는 기지종인 Ph. koreana 다음으로 많은 수가 채집되었으며 체장 80mm., 체폭 4.5mm., 체절수 82개이며 spermathecal pore는 2雙으로서 5/6, 6/7에 位置한다. 특히 xviii 체절 male pore 사이에 雙을 이 불규칙적인 모양의 genital patches 는 본종의 특징이다.

• 보존과학연구
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• s.19
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• pp.3-22
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• 1998

The effect of pH on degradation of paper by some fungi, which able to degrade cellulose, was investigated. Trichoderma koningii, Aspergillus nigerand Penicillium nigulosum were cultured at $28^{\circ}C$ for 16 days in the selective medium (PH3, PH4, PH5, PH6, PH7, PH8, PH9, PH10, PHC) containing paper as substrate. Each paper was pretreated with each pH buffer (pH 3∼pH 10, D.W.)prior to addition to the selective medium. Enzyme activities in the each culture medium were measured spectroph to metrically using C.M.C., Avicel, PNPG as the substrates for endoglucanase, exoglucanase and $\beta$-glucosidase, respectively. In all experimental fungi, the enzyme activities of PH3 and PH9 medium were usually much higher than those of other experimental groups. However in the PH6medium, enzyme activity was lower than other groups. To analyze the concentration and pattern of protein in the each culture medium, the medium was concentrated by lyophilization. The protein concentration of PH3 and PH9 medium were relatively high (T.koningii; 6.31mg, 6,19mg, A.niger; 1.62mg, 1.96mg, P.nigulosum;2.50mg, 2.73mg, respectively), but that of PH6 was relatively low. The protein pattern of each medium was analyzed by using SDS-PAGE and VDS Image Master Analysis Program. The concentrations of bands in the each lane were usually high at lane2 (PH3) and lane8 (PH9) and low at lane5 (PH6). Therefore, the incresed cellulolytic activity of fungus against acidified paper could be result of structural change and deterioration of paper caused by being acidified.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1377-1384
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• 2003

This study investigated the effects of different types of dietary fats and fibers on the concentrations of serum and liver lipids and biochemical index in male Sprague-Dawley rats for 6 weeks. The experimental groups were divided into three groups; 5% corn oil +5% cellulose (CO group),10% corn oil +10% psyllium husk (CO +PH group) and 10% lard +10% psyllium husk (LA+ PH group). Body weight gain and FER (food efficiency ratio%) decreased significantly in the LA+PH group and decreased slightly in the CO+PH group compared with the CO group. Food intake and tissue weights were not significantly different among the dietary groups. Concentrations of serum total cholesterol decreased significantly in the CO + PH group compared with the CO group, but no significant different in the LA + PH group. Concentrations of serum triglyceride and phospholipid decreased significantly in the CO+PH and the LA+PH groups compared with the CO group. Liver cholesterol concentration was significantly decreased in the LA+PH group compared with the CO and the CO+PH groups. Glutamic pyruvic transaminase activity was significantly increased in the LA + PH group compared with the CO and the CO + PH groups. However, lactate dehydrogenase and blood urea nitrogen was significantly decreased in the LA+ PH group compared with the CO and the CO +PH groups. The others biochemical parameters, total protein, albumin, glucose, glutamic oxaloacetic transaminase, alkaline phosphatase were not significantly different among the dietary groups. These results showed that the lowering effect of dietary psyllium husk on serum lipid concentrations was more pronounced in the corn oil -feeding group than in the lard-feeding group as dietary fat source.

• Journal of Food Hygiene and Safety
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• v.26 no.4
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• pp.398-402
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• 2011

Cruciferous vegetables including diindolylmethane (DIM) have been shown to have anticancer activity. Especially, DIM-pPhBr and DIM-pPhF used in this study was reported to have more effective and less toxic effects than DIM. However, there is no report presenting their anti-tumorigenic activity in oral cancer. In the present study, we examined the effects of DIM-pPhBr and DIM-pPhF on the cell proliferation and apoptosis in KB human oral cancer cells. DIM-pPhBr and DIM-pPhF decreased cell proliferation and induced apoptosis evidenced by western blot analysis, DAPI staining and sub-$G_1$ population. This provides the first evidence that DIM-pPhBr and DIM-pPhF originating from Cruciferous vegetables induce apoptotic cell death in human oral cancer cells to inhibit cancer cell proliferation.

• The Korean Journal of Zoology
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• v.12 no.1
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• pp.13-21
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• 1969

韓國産 陸棲貧毛類의 分類學的 硏究의 일련으로서 1965年 $\\sim$ 1966年 및 1968年에 採集된 울릉도 産을 調査한 結果 3科 5屬 10種(Allolobophora caliginosa trapezoides, Eisenia foetida, Pheretima agrestis, Ph. heteropoda, Ph. hilgendorfi, Ph. hupeiensis, Ph. phaselus, Ph. serrata, Ph. sp. 1 Ph. sp. 2)을 報告하였다. 울릉도의 陸棲貧毛類에 관해서는 지금까지 報告된 바 없으므로 이들은 全部 울릉도 未記錄이 되고 이중 Pheretima heteropoda Goto et Hatai 는 韓國 未記錄種이고 Phertima hilgendorfi 는 male pore를 가진 點이 本土産과 比較해서 若干 特異하였다.

• Journal of the Korean Chemical Society
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• v.27 no.5
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• pp.340-344
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• 1983

It has been found that both of the iridium (Ⅰ) complexes, Ir$(ClO_4$)(AN)(CO)$(Ph_3P)_2$(AN = $CH_2$CHCN, $Ph_3P = (C_6H_5)_3$P) and [Ir(AN)(CO)$(Ph_3P)_2]ClO_4$, react with $Cl^-$ to give IrCl(AN)(CO)$(Ph_3P)_2$, and [Ir(AN)(CO)$(Ph_3P)_2]ClO_4$ dissociates AN to yield Ir$(ClO_4)(CO)(Ph_3P)_2$ in the absence of excess AN added, and Ir$(ClO_4)(CO)(Ph_3P)_2$ reacts with $Cl^-$ to produce IrCl(CO)$(Ph_3P)_2$. It is suggested that the catalytic polymerization of AN with Ir$(ClO_4)(AN)(CO)(Ph_3P)_2$ proceeds through the formation of [(CO)(Ph_3P)_2$Ir(-CH=CHCN)(H)($CH_2$=CHCN)]Cl$O_4$ followed by the formation of iridium(alkyl)(alkenyl) type complex which undergoes a reductive elimination to produce the polymer of acrylonitrile.

• Toxicological Research
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• v.19 no.4
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• pp.259-266
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• 2003

Although 'Cinnamon' has been widely used for the food and biophamacy in the world, it's toxicity was not screened completely. Major component of 'cinnamon' is CB-OH and CB-PH. CB-PH has been reported to have antimutagenic effect. To investigate the toxicity of 2-o-Benzoylcinnama-Idehyde (CB-PH), repeated dose (4 weeks) oral toxicity test performed in SD rats. Results of repeated dose oral toxicity tests for 4 weeks (CB-PH; 500, 1000, 2000 mg/kg/day) suggested that the CB-PH treated group showed no significant toxicological findings with body weights, organ weights, hematological and histopathological findings. Therefore, these data indicated that the maximum tolerated dose of CB-PH was 2000 mg above/kg/day in the rats.