• Journal of Life Science
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• v.21 no.9
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• pp.1295-1300
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• 2011

The AtPGR gene is induced by pathogen infection, jasmonic acid and salicylic acid treatment and may therefore play a role in plant defense responses. Arabidopsis thaliana Plasma membrane Glucose-responsive Regulator (AtPGR) was previously isolated from Arabidopsis, which confers glucose insensitivity on plants. To study its biological functions directly, we have characterized both loss-of-function RNAi mutant and gain-of-function transgenic overexpression plants for AtPGR in Arabidopsis. The AtPGR-overexpressing plants displayed enhanced resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae as measured by a significant decrease in both bacterial growth and symptom development as compared to those in wild-type and RNAi plants. The enhanced resistance in the gain-of-function transgenic plants was associated with increased induction of SA-regulated PDF1.2 and JA-regulated PR1 by the bacterial pathogen. Thus, pathogen-induced AtPGR plays a positive role in defense responses to P. syringae.

• The Journal of Korean Obstetrics and Gynecology
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• v.31 no.4
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• pp.1-15
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• 2018

Objectives: Osteoporosis is considered a serious human disease. We developed an extract of mixed herbs containing root of Platycodon grandiflorum (ExMH-PGR), which is expected to be effective in preventing or treating osteoporosis. The aim of this study was to investigate the anti-osteoporotic effect of ExMH-PGR in osteoblastic MC3T3-E1 cells and osteoclastic RAW 264.7 cells. Methods: To examine the anti-osteoporotic effect of ExMH-PGR, osteoblast and osteoclast differentiation were induced and cultured with various concentrations of ExMH-PGR. Alkaline phosphatase (ALP) activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells were analyzed. Tartrate-resistant acid phosphatase (TRAP) activity and the formation of actin ring in RAW 264.7 cells were analyzed. Results: ExMH-PGR at concentration up to $25{\mu}g/mL$ significantly increased ALP activity, collagen synthesis, osteocalcin production, and mineralization in MC3T3-E1 cells. ExMH-PGR at 50 to $200{\mu}g/mL$ significantly inhibited TRAP activity and the formation of actin ring in RAW 264.7 cells. Conclusions: These results demonstrate that ExMH-PGR stimulates osteoblastic activities and inhibits osteoclastic activities in in vitro systems, suggesting that ExMH-PGR might be considered as an anti-osteoporotic candidate for treatment of osteoporosis disease.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.3
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• pp.1-12
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• 2000

식물생장조절제(Plant Growth Regulators, PGRs)를 이용한 예초관리 방법이 들잔디 엽색 및 품 질에 미치는 영향을 규명함으로 잔디 적응력 관점에서 적합한 PGR 종류 및 살포수준을 파 악하고자 본 연구를 시작하였다. 실험은 들잔디 생육이 왕성한 시기인 6월(실험 I)과 7월(실험 II) 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시약제 처리구는 Type I PGR인 amidochlor 3 수준 및 mefluidide 3 수준 과 Type II PGR인 trinexapacethyl 4 수준, 그리고 무처리구를 포함하여 전체 11 처리구였 고, 실험구 배 치는 난괴법 4 반복으로 하였다. 공시한 3종류 PGR 모두 잔디엽색 및 품질에 통계적으로 유의한 차이 가 있었지만, PGR 종류.살포수준 및 생육 상태에 따라 처리효과는 다르게 나타났다. 잔디 엽색 및 품질 은 PGR 살포후 경과기간에 따라 점진적으로 억제되는 경향이었다. 처리 1주후 잔디품질은 대조구에 비 해 큰 차이가 없었지만, 2주에서 4주까지는 PGR 종류 및 살포수준에 따라 유의하게 감소하 여 0.08 ml/ $m^2$ 이상의 trinexapac-ethyl 처리구 및 1.20 ml/$m^2$ 이상의 mefluidide 처리구에서는 가장 크 게 나타났다. 처리 4주부터 대부분 처리구에서 잔디품질이 적정수준 이상으로 회복되기 시작하였다. 본 실험을 통해 잔디관리 수준에서 저관리 잔디지역은 0.08 ml/$m^2$ 수준의 trinexapac-ethyl 처리구가 장기간 생장억제 관점에서 실용성과 경제성 측면에서 들잔디 관리에 효율적인 것으로 판단되었고, 고관리 잔 디지역은 0.04~0.08ml/$m^2$ 수준의 trinexapac-ethyl 처리구 및 0.60~1.20 ml/$m^2$ 수준의 amidochlor 처 리구가 적합 한 것으로 판단되었지만, 계절에 따라 PGR 효과정도가 다르게 나타났다. 따라서, 잔디 관리 시 잔디의 생육상태, 예초 절감 기간, 잔디품질의 기대수준 및 적용할 수 있는 관리수준에 따라 적합한 PGR 종류 선정과 함께 살포시기 및 살포량을 효율적으로 결정하는 것이 필요한 것으로 사료되었다.

• Korean Journal of Pharmacognosy
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• v.36 no.3 s.142
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• pp.209-219
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• 2005

In this study, we carried out the preparing standardization and regulation of processed Glycyrrhizae Radix (PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for the purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dry on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increased as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA, obtained from heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGA in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on $CCl_4-intoxicated$ rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was $150^{\circ}C$ for 80 min.

• Development and Reproduction
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• v.23 no.3
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• pp.277-284
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• 2019

ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout ($Pgr^{cre/+}Arid1a^{f/f}$) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of $Pgr^{cre/+}Arid1a^{f/f}$ mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RT-qPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the $Pgr^{cre/+}Arid1a^{f/f}$ mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, $Pgr^{cre/+}Arid1a^{f/f}$, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the $Pgr^{cre/+}Arid1a^{f/f}$ and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.

• Asian Journal of Turfgrass Science
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• v.3 no.1
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• pp.39-52
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• 1989

Native Kerean lawngrass (Zeysia japonica steud.) and Manilagrass ( Zeysia matrella Merr.) are frequently used as sport turf and lawn in Kerea and ether Asian countries because of its excellent summer qualities and superior winter-hardiness compared to ether warm-season turfgrass . The objective of this study was to investigate the effect of the plant growth retardants ( PGR ) en the inhibition (d the growth of the two Kerean native turfgrasses. Kerean lawngrass and Manilagrass were treated with [2 RS , 3 RS] -1- [4- chlorophenyl ] -4, 4-dimethyl -2- [1 H -1, 2, 4- tiazol -1- y] pentan -3- ol ( PP -333) at 93 and 930 gha -j ; 5-[4- chlorphenyl ]-3, 4, 5, 9, 10- peiitaaza - tetracyclo [5, 4, 1, 0 2 6 . o ~'~I -3, 9- diene ( BAS -106) at 16 and 160 gha ; [ E I-] - cyclohexyl -4, 4- dimethyl -2- [1, 2, 4- triazol -1- yl ]-1-pentene - 3- ol ( NTN -821) at 56 and 560 gha ', and 4- chloro -2- [$\alpha$- hydrozybenzy I isonicotin anilide ( CGR -811) at 92 gha in pot study . All PGR treatments were effective in reducing the plant height, with the exception of BAS -106 at 16 gha for Korean lawngrass ; however , all increased quality to some degree , with the exception of PP -333 at 93 gha . The low PGR rates significantly increased Korean lawngrass height during late jtily aitd mid-August . On the other hand , BAS -106 significantly increased Manilagrass height {rom 24 Septeml)er onwards . All PGR treatments haol higher turfgrass quality than untreatments , and also the high rates of PGIt treatments were more effective in increasing quality than the low rates observed on 8 October . Meanwhile , Manilagrass quality was increased to some extent than Korean lawegrass . PP - 333 was significantly effective in reducing clipping yield and the same results were found with the application of NTN -821 at 560 gha -l for both Korean lawngrass and Manilagrass , BAS -106 at 160 gha for Korean lawegrass , and CGR -811 at 92 gha for Manilagrass . The high PGR rates had higher turfgrass shoot weight observed on 8 October than the low rates , and there were little increases obtained with the low rates of PP -333 for Korean lawngrass and BAS - 1 06 for Manilagrass . Effeets on stolon growth varied between growth retardants , and between retardant treatments ; the high rates of PGR as well as the low rate of NTN -821 elongated stolon of Korean lawngrass and manoilagrass . However , PP -333 at 93 gha and BAS -106 at 16 gha - shortened stolons of Korean lawugrass , resulting in inhibition or little difference of stolon dry weight . NTN -821 at 560 gha and CGR -811 had no positive effect on the stolon length of Manilagrass . 101) -333 and the low rate of BAS -106 and NTN -821 decreased root weight of Korean lawng - rass All PGR rates had negative effect on the root weight of Manilagrass with the exception of BAS -106 CR -811 and the high rate of NTN -821 which made no difference in root weight between retarolant treatments and the control .

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.7
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• pp.3041-3044
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• 2014

The aim of this work was to analyze methylation of the promoter sites of the ESR1 and PGR genes and to determine correlations with immunohistochemical expression of estrogen and progesterone receptors in ductal and lobular breast cancers. An observational, descriptive, molecular study was conducted on 20 ductal and 20 lobular breast cancer samples with immunohistochemical determination of estrogen and progesterone receptor expression. The methylation analysis of ESR1 and PGR promoter sites was carried-out by methylation-specific PCR. For correlation analysis, Kendall's tau coefficient was determined. Positive correlations were found between estrogen and progesterone receptors, estrogen receptor and unmethylated progesterone receptor, progesterone receptor, and unmethylated progesterone receptor. Negative correlations were found between estrogen receptor and methylated progesterone receptor, progesterone receptor and methylated progesterone receptor, methylated and unmethylated estrogen receptor, and methylated and unmethylated progesterone receptor. The results suggest that methylation of promoter sites of ESR1 and PGR is a relatively uncommon event in ductal and lobular breast cancer, and also suggest that the determination of epigenetic states of ESR1 and PGR could represent an alternative or complement to the histopathological expression analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1176-1182
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• 2018

Objective: Progesterone receptor (PGR) and AT-rich interactive domain 1A (ARID1A) have important roles in the establishment and maintenance of pregnancy in the uterus. In present studies, we examined the expression of mitochondrial tumor suppressor 1 (MTUS1) in the murine uterus during early pregnancy as well as in response to ovarian steroid hormone treatment. Methods: We performed quantitative reverse transcription polymerase chain reaction and immunohistochemistry analysis to investigate the regulation of MTUS1 by ARID1A and determined expression patterns of MTUS1 in the uterus during early pregnancy. Results: The expression of MTUS1 was detected on day 0.5 of gestation (GD 0.5) and then gradually increased until GD 3.5 in the luminal and glandular epithelium. However, the expression of MTUS1 was significantly reduced in the uterine epithelial cells of $Pgr^{cre/+}Arid1a^{f/f}$ and Pgr knockout (PRKO) mice at GD 3.5. Furthermore, MTUS1 expression was remarkably induced after P4 treatment in the luminal and glandular epithelium of the wild-type mice. However, the induction of MTUS1 expression was not detected in uteri of $Pgr^{cre/+}Arid1a^{f/f}$ or PRKO mice treated with P4. Conclusion: These results suggest that MTUS1 is a novel target gene by ARID1A and PGR in the uterine epithelial cells.

• Applied Chemistry for Engineering
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• v.21 no.2
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• pp.148-153
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• 2010

This study was carried out to investigate the effect of extraction methods on the physicochemical characteristics using seed oil of wild green tea (Camellia sinensis). When the solvent extraction method after grinding and steam treatment (SGS) was used for oil extraction, the yield was highest. The specific gravity was a range of $0.91{\sim}0.94g/cm^{3}$ irrespective of extraction methods of oil. However, the light in the solvent extraction method after grinding (SG), the red in the pressure extraction method after grinding and roasting treatment (PGR), and the yellow in SG method were highest. Among various fatty acids, the concentrations of C16 : 0, C18 : 1 and C18 : 2 were highest, irrespective of extraction methods. Especially, the C16 : 0 concentration was in the order of SG (34.78%), SGS (23.04%), and PRGS method (23.01%), the C18 : 1 concentration was in the order of PGR (43.35%), SGS (42.7%), SG method (39.0%), and in the case of C18 : 2, it was in order of PGR (23.15%), SGS (23.03%), and SG method (15.01%). The saturated fatty acid concentration was in the order of SG (40.59%), PGR (31.61%), and SGS method (30.1%). On the other hand, in the case of the unsaturated fatty acid, it was in the order of SGS (69.9%), PGR (68.39%), and SG method (59.41%). The acid values in the SGS and SG method after 10 days of storage were in the range of 6~8 mgKOH/g. However, in the case of PGR method, it was increased with the increase of storage time and was 49.3 mgKOH/g after 60 days. The peroxide values in the SGS and SG method were in the range of 60~100 mEq/g from 10 to 60 days of storage. On the other hand, when the storage time was increased from 10 to 30 days, it was sharply increased from 60 to 240 mEq/g. The rancidity was in the order of $Fe^{2+}$, $Cu^{2+}$, $Cr^{2+}$, $Zn^{2+}$ and $Ni^{2+}$, irrespective of extraction methods. Especially, when butylated hydroxyanisole (BHA) was added into oil containing 1.0 ppm of <$Fe^{2+}$, the peroxide value was decreased from 539.4 to 216.6%. These results show that seed oil of Camellia sinensis grown in Iksan can be applied as sources for cosmetics, detergents, food, and pharmaceuticals.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.353-360
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• 2016

Effect of heavy metals(Cd, Cu, Zn) on the survival and population growth rates(PGR) of marine rotifer, Brachionus plicatilis were examined. B. plicatilis were exposed to Cd, Cu and Zn for 24 h to determine their survival and 72 h to determine their PGR. Survival rates in the control groups were greater than 90%. They were decreased with increasing concentrations of Cd, Cu and Zn. Survival rates were reduced in a concentration-dependent manner. Significant reduction in survival rates after exposure to Cd, Cu and Zn at concentration greater than 40.00, 0.13 and $10.00mg\;L^{-1}$, respectively. PGR in the control groups were greater than 0.50. They were decreased with increasing concentrations of heavy metals. PGR were reduced in a concentration-dependent manner. Significant reduction in PGR after exposure to Cd, Cu and Zn occurred at concentration greater than 12.5, 0.06 and $1.00mg\;L^{-1}$, respectively. The order of heavy metal toxicity based on PGR was Cu>Zn>Cd, with $EC_{50}$ (50% Effective Concentration) values of 0.12, 6.15 and $21.41mg\;L^{-1}$, respectively. The lowest-observed-effective-concentrations(LOEC) of PGR after exposure to Cd, Cu and Zn were 12.50, 0.06 and $1.00mg\;L^{-1}$, respectively. The No-observed-effective-concentrations(NOEC) of PGR after exposure to Cd, Cu and Zn were 6.25, 0.03 and $0.01mg\;L^{-1}$, respectively, in marine ecosystems have toxic effects on PGR of B. plicatilis. These results suggest that the PGR of B. plicatilis are useful tool to assess the effect of heavy metals on primary consumers in marine natural ecosystems.