• Journal of Life Science
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• v.27 no.10
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• pp.1145-1151
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• 2017

In this study, we report on a simple, efficient method for obtaining penicillin G amidase (PGA) from recombinant Escherichia coli using a formulation mixed with detergent and lysozyme. Research was conducted on the extraction efficiency of PGA from the periplasmic space in cells in terms of the type of detergent, detergent concentration, pH, reaction time, and temperature of permeabilization. The extraction yield of PGA in the formulated surfactant/lysozyme treatment was increased by approximately (55-65 U/ml) in comparison with that in the single surfactant treatment. The released PGA solution was concentrated and exchanged with buffer using an ultrafiltration (U/F) system. The yields of diatomite filtration, membrane filtration (M/F), and U/F were 69.7%, 93.8%, and 77.3%, respectively. A total of 212 KU of PGA was recovered. At the 25-L culture scale, the overall yield of extraction using the mixed surfactant/lysozyme method was 49.2%. The specific activity of extracted PGA was 11 U/mg in protein. The concentrated PGA solution was immobilized on microporous silica beads without further purification of PGA. The total immobilization yield of PGA on the resin was 48.7%, while the enzyme activity was 101 U/g. The immobilized PGA was successfully used to produce 6-APA from penicillin G. Our results indicated that a simple extraction method from periplasmic space in E. coli may be used for the commercial scale production of ${\beta}-lactam$ antibiotics using immobilized PGA.

• Journal of the Earthquake Engineering Society of Korea
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• v.15 no.5
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• pp.35-44
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• 2011

PGA (Peak Ground Acceleration) is the parameter which indicates the peak value for strong ground motion and is mainly due to the intensity of the seismic wave. Usually, seismic waves can consist of different characteristics and can have different effects on structures. Therefore, it may be undesirable that the effects of a seismic wave are evaluated only based on the PGA. In this study, time history analysis was executed with a single degree of freedom model for inelastic seismic analysis. The numerical model was assumed to be a perfect elasto-plastic model. Input accelerations were made with El Centro NS (1940), other earthquake records and artificial earthquakes. The displacement ductility demand and cumulative dissipated energy, which were calculated from other artificial earthquakes, were compared. As a result, different responses from other seismic waves which have the same PGA were identified. Therefore, an index which could reflect both seismic and structural characteristics is needed. The SI (Spectrum Intensity) scale which could be obtained from integration by parts of the velocity response spectrum could be an index reflecting the inelastic seismic response of structures. It can be possible to identify from correlation analysis among the SI scale, displacement ductility demand and cumulative dissipated energy that the SI scale is sufficient to be an index for the inelastic response of structures under seismic conditions.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.231-236
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• 2004

The inexpensive large-scale production of pure PGA (Penicillin G Acylase) has been a commercial goal. PGA has been used as a model enzyme in the development of simple one-step purification methods. In this study, the purification of poly-His tagged PGA protein secreted into the periplasmic space was carried out by using immobilized metal-ion affinity chromatography (IMAC). The PGA gene was obtained from E. coli ATCC 11105. Codons encoding histidines were fused at the C-terminus of the PGA gene by PCR. E. coli JM109 harboring pPGA-HIS6 vector produced active his-tagged acylases in the presence of lac promoter during cultivation at $26^{\circ}C$. The maximum specific activity of the acylase purified by using one-step chromatography after osmotic shock was 38.5 U/mg and was recovered with the yield of 70％. Both 23 kDa ($\alpha$) and 62 kDa ($\beta$) subunits were recovered by using IMAC with just C-terminus tagging of the $\beta$ subunit. The purification of the periplasmic fraction by osmotic shock and that of purified acylase was increased by 2.6-fold and 19-fold, respectively, compared to the crude extract.

• Proceedings of the Korea Concrete Institute Conference
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• 1997.10a
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• pp.581-586
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• 1997

The objective of this study is to investigate the behavior of a 1/5-scale 3-story nonductile reinforced concrete frame subjected to earthquake excitation. For this purpose, Taft N21E earthquake accelerogram was simulated by using 3m${\times}$5m shaking table. When the input acceleration is compared to that of output, it can be found that simulation of shaking table is excellent. From the results of test with Taft N21E earthquake accelerogram adjusted to peak ground acceleration(PGA) 0.06g and 0.12g(maximum acceleration in korea seismic code) the model responded in elastic behavior and it is found that the existing building in our country are safe against the levels of PGA 0.06g and 0.12g.

• Computers and Concrete
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• v.13 no.1
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• pp.1-16
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• 2014

To evaluate the behavior of the advanced unbonded pre-stressed concrete containment vessel (UPCCV) for one typical China nuclear power plant under Japan's March 11 earthquake, five nonlinear time history analysis and a nonlinear static analysis of a 1:10 scale UPCCV structure have been carried out with MSC.MARC finite element program. Comparisons between the analytical and experimental results demonstrated that the developed finite element model can predict the earthquake behavior of the UPCCV with fair accuracy. The responses of the 1:10 scale UPCCV subjected to the 11 March 2011 Japan earthquakes recorded at the MYG003 station with the peak ground acceleration (PGA) of 781 gal and at the MYG013 station with the PGA of 982 gal were predicted by the dynamic analysis. Finally, a static analysis was performed to seek the ultimate load carrying capacity for the 1:10 scale UPCCV.

• Structural Engineering and Mechanics
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• v.28 no.6
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• pp.727-747
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• 2008

In the framework of the SPEAR (Seismic PErformance Assessment and Rehabilitation) research Project, an under-designed three storey RC frame structure, designed to sustain only gravity loads, was subjected, in three different configurations 'as-built', Fiber Reinforced Polymer (FRP) retrofitted and rehabilitated by reinforced concrete (RC) jacketing, to a series of bi-directional pseudodynamic (PsD) tests under different values of peak ground acceleration (PGA) (from a minimum of 0.20g to a maximum of 0.30g). The seismic deficiencies exhibited by the 'as-built' structure after the test at PGA level of 0.20g were confirmed by a post - test assessment of the structural seismic capacity performed by a nonlinear static pushover analysis implemented on the structure lumped plasticity model. To improve the seismic performance of the 'as-built' structure', two rehabilitation interventions by using either FRP laminates or RC jacketing were designed. Assumptions for the analytical modeling, design criteria and calculation procedures along with local and global intervention measures and their installation details are herein presented and discussed. Nonlinear static pushover analyses for the assessment of the theoretical seismic capacity of the structure in each retrofitted configuration were performed and compared with the experimental outcomes.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.10a
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• pp.541-546
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• 1998

The objective of this research is to observe the actual response of low-rise nonseismic moment-resisting infilled reinforced concrete frame subjected to varied levels of earthquake ground motions. First of all, the reduction scale for the model was determined as 1 : 5 considering the capacity of the shaking table to be used. This model was, then, subjected to the shaking table motions simulating Taft N21E component earthquake ground motions, whose peak ground accelerations(PGA`s) were modified to 0.12g, 0.2g, 0.3g, and 0.4g. The global behavior and failure mode were observed. The lateral accelerations and displacements at each story and local deformations at the critical portions of structure were measured. Before and after each earthquake simulation test, free vibration tests were performed to find the changes in the natural period of the model.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.05a
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• pp.139-144
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• 2001

The objective of this study is to investigate the behavior of 1/12 scale upper-wall lower-frame reinforced concrete structure subjected to earthquake excitations. For this purpose, Taft N21E earthquake accelerogram was simulated by using 4m$\times$4m shaking table. When the input acceleration is compared to that of output, it was found that simulation of shaking table is satisfactory. From the test results with peak ground acceleration(PGA) 0.22g, which corresponds to 0.11g in prototype by the similitude law, it can be observed that the model responded in elastic behavior and that large interstory drift occurred at the lower part of the structure.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.6
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• pp.1185-1194
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• 1997

It is necessary to control the frame memory to capture, edit and display images. This paper presents the free-scale image capture processor size of which is user-defined, compared to the conventional image capture processor size of which is fixed 1/2, 1/4 and full size. User-defined scale data is fed into this system, which generates the gating pulses and gates the inputted image data. This system also controls the 4M DRAM instead of frame meamory. And stored gated image data are displayed on the TV monitor. We designed the scalable image capture parts and DRAM controller with ACTEL FPGAs, simulated the circuits with Viewlogic and fusing ACTEL A1020B chips. We confirmed the whole operation with beadboard which composed of "Philips TV Chipset" and designed FPGA chips.PGA chips.

• CELLMED
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• v.10 no.2
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• pp.15.1-15.9
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• 2020

Introduction: Buthūr Labaniyya (Acne vulgaris) is a multifactorial disorder of the pilosebaceous units characterized by non-inflammatory and inflammatory clinical lesions. Several Unani medications have been used for centuries to treat acne. Objectives: Evaluation of safety and efficacy of Tila-i Muhāsā in patients with acne vulgaris Materials and Methods: This clinical study was conducted in patients with acne vulgaris. Patients applied Tila-i Muhāsā or 5% Benzoyl Peroxide (BPO) once daily for 6 weeks. Assessment of efficacy was carried out by Global Acne Grading System (GAGS) and Patient Global Assessment (PGA) scores. In addition, overall severity of acne was evaluated on a 5-point Cook's acne grading scale using photographic standards. Assessment of safety was performed through adverse drug reactions, local dermal tolerability, vital signs, and routine laboratory investigations. Results: A total of 60 patients (30 in each group) completed 6 weeks of treatment. The mean percentage reduction in GAGS score at 6 weeks from baseline in Unani group (66.97%) and BPO group (59.09%) was statistically significant (P<0.0001). After 6 weeks of therapy, the mean percentage reduction in PGA score compared to baseline in Unani group (57.44%) and BPO group (50.23%) was statistically significant (P<0.0001). No serious adverse events were reported in both groups; however, mild adverse events occurred more frequently in BPO group (30%) compared to Unani group (10%). Conclusion: Tila-i Muhāsā was found to be effective and safe in the treatment of acne vulgaris. However, further clinical studies with larger sample size and longer duration of therapy need to be conducted.