• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.2
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• pp.138-142
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• 1999

An experiment was carried out to find out the changes in morphology, sugars, and $\alpha$-amylase activity during the priming of rice seeds (Oryza sativa L. cv. ＇Ilpumbyeo＇). For priming, seeds were soaked in -0.6 MPa PEG solution at 15$^{\circ}C$ for 4 days (properly primed) and at $25^{\circ}C$ for 4 and 10 days (over-primed) and dried at room temperature. The size of coleoptile and differentiated leaves of properly primed seeds were bigger and coleoptile was separated from the other part of embryo compared with non-primed and over-primed seeds. As priming of seeds advanced, compound starch grains in the endosperm disintegrated into tiny starch granules, and small holes were found in the tiny starch granules and a cavities developed between embryo and endosperm. The radicle and plumule of properly primed germinating seeds developed faster than non-primed and overprimed germinating seeds. Sucrose, maltose, and raffinose contents of properly primed seeds decreased, while content of glucose and fructose and $\alpha$-amylase activity increased. However, sugar content and $\alpha$-amylase activity of over-primed seeds were lower compared with non-primed seeds or properly primed seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.145-156
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• 1996

The effects of priming with different polyethylene glycol (PEG 6, 000) solutions on the germination, emergence characteristics and early plant growth in rice, barley and wheat were investigated. Rice, barley and wheat seeds were subjected to various priming conditions of osmotic potentials (-0. 75, -1.00 and -1.50 MPa) of PEG, and incubation period were 5 days at 25, 20, 2$0^{\circ}C$, respectively. The percentage of water absorption was highest in barely, and lowest in rice, and all the treatments enhanced water absorption in barley, but showed no significant effects in rice and wheat. Respiration quotient was lower than none PEG treatment seeds, and those of barley and wheat were higher than rice regardless of PEG concentration, and that of rice increased with high PEG concentration. Total germination percentage of osmoconditioning seeds with PEG was higher than that of none treatment seed, and those of barley and wheat were significant. The artificially deteriorated seeds with PEG treatment seeds after ageing treatment could recover to nearly the same germination level as that of the control seeds. The effects of coating polymer were higher than osmoconditioning with PEG, and germination characteristics in rice showed varietal difference at PVP and waterlock at recoated seeds after PEG treatment. Osmoconditioning with PEG reduced mean germination and emergence time, but there was no difference among PEG concentrations. The plant height of PEG treaed seed in rice was taller and those of barely and wheat showed varietal difference, and those of polymer-coated seed after PEG treatment were different among the polymers. The dry weight of PEG treatment were different among the crops, and those were increased with the high PEG concentration. The emergence percentage of PEG-treated seed were higher than none-treated seed, and those were decreased with the increased PEG concentration, and the highest emergence percentage of rice, barley and wheat were 90, 50 and 50% soil moisture content, respectively. The time to emergence in rice was longer than barley and wheat, and those in rice was shortened in high soil moisture content, and barley and wheat were shortened in low soil moisture content.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.3
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• pp.194-198
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• 1998

An experiment was carried out to find out the priming effects of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) on. the seedling establishment and early emergence under excess soil moisture conditions. Seeds were primed by soaking in -0.6 MPa polyethylene glycol (PEG) solution at $25^{\circ}C$ for 4 days. The primed seeds were sown in soils with various soil moistures (60, 80, 100, 120, and 140% field capacity) at 17 and $25^{\circ}C$, respectively. Germination and emergence rates, plumule height, and radicle length of primed seeds were higher than those of untreated seeds at any soil moisture and temperature examined. The time from planting to 50% germination ($T_{50}$) of primed seeds was less than that of untreated seeds by 0.9～3.7 days. Germination rate, emergence rate, plumule height, and radicle length were highest at the soil moisture of 80% field capacity among the soil moistures. Priming effects of rice seeds on germination and emergence rates were more prominent under the unfavorable soil moistures (60, 100, 120, and 140% field capacity) than those under the optimum soil moisture condition (80% field capacity). However, priming effects on seedling growth were greater at near optimum soil moisture compared with too lower or higher soil moistures. Therefore, these findings suggest that priming of rice seeds may be a useful way for better seedling establishment under the adverse soil conditions.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.214-214
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• 2017

Seeds of Tomato (Lycopersicon esculentum Mill.) have demanded high quality because of their high cost of seed. The optimization of the seed priming techniques that have positive effect on fast and uniform germination becomes important at the commercial level. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. In this study, osmotic potentials of priming solution and germination characteristics of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Tomato seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of PEG 8000 (17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $Na_2SO_4$, and $K_2SO_4$ (100, 200, 300mM). The seeds were treated at $20^{\circ}C$ for 2, 4, and 6 days. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$. Four replications of 25 seeds per each treatments were placed in 10-cm petri dishes containing two filter papers and 3 ml of $dH_2O$ and incubated at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$ and seedlings evaluated for abnormality after 14 days of incubation. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.86$). Seeds primed in 100mM $KNO_3$ resulted the highest germination rate (GR, $63.9 %{\cdot}day^{-1}$) and lowest mean germination time (MGT, 2.0 days) comparing to untreated control ($23.9%{\cdot}day^{-1}$ of germination rate and 4.1 days of MGT) at $20/30^{\circ}C$, even though 96% of germination percentage were not different. Seeds primed in 100mM $KNO_3$ (${\psi}=-0.45MPa$) for 4 days showed ${\psi}=-0.38MPa$. Priming in $Na_2SO_4$, $K_2SO_4$, and PEG solution for 6 days improved MGT and GR, but not significantly than 4 days of treatment. Additionally, stepwise osmotic solution treatment with 100mM and 300mM concentration for 6 day did not showed differences with single treatment. In relation to osmotic potentials, identical osmotic potential in different inorganic salt solution showed different effect on germination characteristics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.265-273
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• 1995

Seeds of chicory were collected and selected with good germination, emergence and productivity. To develop chicory as a new forage crop seeds were primed with polyethylene glycol (PEG 6000) and coated with some chemicals, and tested in laboratory and field. The results obtained are summarized as follows: 1. Among the seed lines collected, PUlgAH, PU21TH, PU37CH and PU30TK were germinated more quickly and took less time for germination than the others. 2. PUI8AH was the best in emergence and growth although PU21TH showed superior germinative ability among the lines collected. 3. Chicory seeds primed with 20~25% PEG solution for three days at $10^{\circ}C$, or the seeds treated with 25% PEG solution at $15^{\circ}C$ regardless the treatment period showed good germination performances. 4. Osmotic priming with PEG accelerated the germination of chicory, but not in the field. 5. Coated seeds geminated more slowly in Petridishes but showed superior emergence and growth to those of primed or untreated seeds. Treatment 3 showed the best performances in pot experiment. 6. Coated seeds showed remarkable increase in establishment and dry matter yield especially in sod sowing compared to the other sowing method. 7. Most nutrient contents except iron and manganese of chicory were much higher than those of mixed pasture .

• Plant Resources
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• v.2 no.2
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• pp.88-95
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• 1999

These experiments were conducted to evaluate the variability of seed germination and seedling growth with different levels of polyethylene glycol(PEG 6,000) solution in onion seed. Average germination percentage of seed primed in PEG solution with 1.00 and 0.75 MPa was higher than control, and that of seed primed in 1.50MPa was lower than unprimed control. Germination percentage(GP) of seed primed for 5 days was highest, and as the primed days become long, the GP was decreased. The GP of airation seed during the primed was higher than that of unairation seed, about 5% , respectively. The GP of washed seed after primed was higher than that of unwashed seed, but that of redried seed after primed was lower than that of the others. The highest GP cultivar was Chunjoogoohyung and the lowest GP cultivar was Seouldego in unredried seed after primed, but Chunjoojoonggo was highest and Jungpoonwhang was the lowest cultivar in redried seed after primed. As the PEG concentration increased, the seedling length(SL) was shortened, and seed primed for 15 days was longer than other treatments. The SL of primed seed was similar to GP. The SL of washed seed after primed was longer than that of others, but that of redried seed after primed was shortest among the others. The SL of Chunjoojoonggo and Nongwoodego was longest and Seouldego was shortest among the cultivars in unredried seed after primed, but that of Chunjoogoohyung and Chunjoojoonggo was longest and Seouldego was the shortest cultivars in redried seed after primed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.2
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• pp.128-138
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• 1994

To improve seed vigour in rice, barley and soybean, several methods of presowing treatment, using chemicals and priming in polyethylene glycol solution, were investigated. Gibberellic acid($GA_3$) slightly improved germination of rice, but other chemical treatments showed no beneficial effect on seed vigour. Aged seeds were primed in polyethylene glycol solution then rinsed and germinated with drying back. In general mean germination time increased and percentage germination decreased with in- creasing water potential of the priming solution, but there were no significant effects on spread of germination times. Priming also showed no marked improvement in germination under cold, wet, or osmotic conditions. None of the treatments used was successful in practically improving the seed germination and vigour of the tested crops. However, seed treated with GA$_3$ gave the best overall germination response.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.322-325
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• 2003

Controlled hydration treatment of tobacco seeds enhanced seed performance greatly without additional materials associated with conventional osmotic or solid matrix priming technique. The seeds were hydrated by adding water to a level from 10 to 60% by 5% increments and incubated for 8 days at $25^{\circ}C$. After the treatment, the seeds were dried to the original seed moisture content under $20^{\circ}C$ for 72 hours. The moisture content of tobacco seeds equivalent to 35% by the hydration treatment gave the greatest improvement in germination rate and speed compared to untreated or polyethylene glycol (PEG) primed seeds, especially at low temperature of $15^{\circ}C$.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.505-511
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• 1994

Seeds of Rigitaeda pine tree(Pinus rigida${\times}$P. taeda) was primed with polyethylene glycol(PEG-6000) under different PEG concentrations, treatment period, and temperatures to test uniformity of germination. Coated seeds and PEG treated seeds were sown to compare germination, emergence, establishment, and seedling growth. The results obtained are summarized as follows : 1. As the concentration of PEG increased, as total germination percentage of pine tree was increased. Total germination percentage of pine tree seeds primed with PEG at 15 or $20^{\circ}C$ was better than the seeds treated at $10^{\circ}C$ compared to untreated seeds. 2. Germination rate was improved as primed period long and level of PEG concentration high. 3. Maximum germination rate was high with long PEG treatment period, and markedly improved when the seeds were primed at $20^{\circ}C$ 4. Priming with PEG at $20^{\circ}C$ for 12 days reduced time taken for germination. 5. Osmoconditioned seeds accelerated germination under drought condition with injurious effect of coated seeds by some chemicals. 6. There were much differences in establishment and dry matter production between drill and oversowing method, and primed seeds showed better performances than the coated seeds. Coating effects to seeds were better under drought soil condition rather than moderate moisture condition.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.216-216
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• 2017

Primed seeds germinate faster and uniformly, since these have already started imbibition and metabolism requiring to complete germination. Several factors such as solution composition, osmotic potential, and treatment duration affect seed priming response. Water potentials of priming solution and germination response of primed seed were investigated to clarify the effects different inorganic salt types and the duration. Pepper seeds were primed in osmotic solutions that were osmotic potential ranged -1.54 to -0.45 MPa in an aerated solution of polyethylene glycol 8000 (PEG; 17%, 22%, 27%), and inorganic salt solution of $KNO_3$, $K_2SO_4$, and $Na_2SO_4$ (100, 200, 300mM) in aerated condition. The seeds were treated at $20^{\circ}C$ for 4, 6 day (D) and stepwise duration combined concentration of 100mM (2 or 4 days) and 300mM(2 or 4days). After soaking treatment, seeds were washed with distilled water and then were dried to approximately 6% moisture content in dry chamber at $25^{\circ}C$. The germination of seed was characterized by ISAT rules at $20^{\circ}C/30^{\circ}C$ and $15^{\circ}C$. Total germination percentage (GP), mean germination time (MGT), germination uniformity (GU), germination rate (GR), and health seedling percent (HS) were calculated on the germinated seed in a 14-day period. Seed water potential (${\psi}$) was correlated with water potential of priming solution ($r^2=0.84$). The effect of seed priming on germination varies with inorganic salt and organic agents (PEG). Germination percentage (GP, 77 and 73%), GR (21.3 and $19.4 %{\cdot}day^{-1}$), and HS (58 and 52 %) was greatest and lowest MGT (4.2 and 4.8 day) when they were primed in 100mM $K_2SO_4$ (${\psi}=-0.45MPa$), and 100mM $KNO_3$ (${\psi}=-0.52MPa$) for 6 days compared to untreated control (67% GP, $19.7%{\cdot}day^{-1}\;GR$, 18% HS, and 10.8 day MGT) in $15^{\circ}C$ chamber. Water potential less then -1.2MPa of osmotic solution resulted lower GP, GR and HS and in $K_2SO_4$ and $KNO_3$. However, stepwise treatment of water potential that changed solution as 100mM for 4 D + 300mM for 2 D or 300mM for 4 D + 100mM for 2 D) were not better than single concentration alone in germination characteristics.