• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.289-292
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• 1999

마늘은 백합과 식물로서 인류의 식생활에서 중요한 역할을 할 뿐 아니라 최근에는 항암작용을 가진 것으로도 알려져 세계적으로 많은 연구가 수행되고 있다. 그러나 같은 백합과 식물인 양파의 경우 과량 섭취시 개에서 용혈성 빈혈이 유발된다는 것은 잘 알려진 사실이지만 마늘의 경우 이러한 부작용에 관한 보고는 전혀 없는 실정이다. 따라서 본 연구에서는 체중 25 kg 이상의 성견을 대상으로 마늘 추출액을 투여한 결과 다음과 같은 성적을 얻었다. 총적혈구수(RBC), 헤모글로빈(Hb)함량, 혈구용적(PCV), 평균적혈구용적(MCV), 평균적혈구혈색소농도(MCHC)는 실험기간 중 유의한(P＜0.05) 감소경향을 나타내었다. GSH 함량은 6일째 유의한(P<0.01)증가를 나타내었다. 본 실험 성적의 결과 과량의 마늘 투여시에도 양파에서와 동일한 용혈성 빈혈이 발생되는 것으로 생각된다.

• Journal of Veterinary Clinics
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• v.4 no.2
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• pp.505-514
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• 1987

Babesia gibsoni infection was diagnosed and treated in three hunting dogs which were hospitalized to the Veterinary Teaching Hospital, College of Veterinary Medicine, Seoul National University between April 4 and August 29, 1987. All three dogs revealed severe anemia, hemoglobinuria, splenomegaly and markedly decreased PCV, RBC count and hemoglobin. The anemia was regenerative, as characterized by increased numbers of nucleated erythrocytes, polychromasia, anisocytosis, reticulocytosis. B. gibsoni was identified by examination of blood smear stained with Giemsa stain. The forms of B. gibsoni identified in this report were pleomorphic such as singnet ring, oval, comma, dot and elongated forms. The maximal percentages of erythrocytes infected with one or more B. gibsoni organisms were 39%, 20% and 40%, respectively. The Tick, Haemophysalis longicornis was assumed to be the vector of babesiosis in these cases. Specific treatment consisted of diminazene aceturate and supportive treatment consisted of whole blood transfusion, lactated Ringer's solution, vitamin B complex and broad spectrum antibiotics. All three dogs had convalesced successfully after treatment.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.505-509
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• 2000

A five-month old female dog was presented to Teaching Anilmal Hospital, Gyeongasng National University for femur fracture. Previously the dog had been presented to a local hospital. Orthopedic and blood examinations were conducted. There was absent response of conscious propri- oception. The dog stood knuckled onto the digits and had hock dropped, but showed no117a1 values in WBC.RBC, Hb, PCV, BUN, Creatine, Glucose, Cholesterol, AST and ALT. Plain radioaraphs were taken and there were abundant periosteal reaction and malalignment of fragments. The dog was anesthetized and prepared for aseptic surgery. Joshi external fixator and half pins were applied(uni lateral). AT 8 weeks after surgery, the pins were removed from the bone. The fracture was well healed wish no evidence of original fracture line. but mild muscle atropy and shortening of femur were found.

• Korean Journal of Veterinary Research
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• v.28 no.2
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• pp.285-297
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• 1988

The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328(♂ 92, ♂ 236) healthy Jindo dogs in Jindo area. The results obtained in this study were summarized as follows: 1. The mean values of red blood cell(RBC) count, hemoglobin(Hb) content and packed cell volume(PCV) in the age group of less than one year were 6.77(♂ 6.80, ♂ 6.76)${\times}10^6/{\mu}l$, 13.14 (♂ 13.53, ♂ 12.99)g/100ml and 43.28(♂ 44.47, ♂ 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(♂ 7.44, ♂ 7.42)${\times}10^6/{\mu}l$, 14.98(♂ 15.56, ♂ 14.76)g/100ml and 47.18 (♂ 48.43, ♂ 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PCV appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. 2. The mean values of mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin content(MCHC) in the age group of less than one year were 63.93(♂ 65.40, ♂ 63.30)fl, 19.41(♂ 19.90, ♂ 19.22) pg and 30.36(♂ 30.43, ♂ 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(♂ 65.09, ♂ 62.95)fl, 20. 19(♂ 20.91, ♂ 19.89) pg and 31.75(♂ 32.13, ♂ 31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. 3. The mean values of white blood cell(WBC) count in the age group of less than one year were 14,356(♂ 13,878, ♂ 14,551)/${\mu}l$, whereas the values of WBC count in the age group of one year and more were 13,394(♂ 12,656, ♂ 13,672)/${\mu}l$. The WBC count appeared to be higher in the female than in the male. No differences were found between age groups in the WBC count. 4. In WBC differential count the mean percentage of lymphocyte, monocyte, neutrophil and eosinophil of the age group less than one year were 33.45(♂ 31. 64, ♂ 34.18), 2.77(♂ 3.00, ♂ 2.68), 57.40(♂ 58.18, ♂ 57.08) and 6.38(♂ 7.18, ♂ 6.06)% respectively, while those of the age group one year and more were 30. 22(♂ 28.38, ♂ 30.90), 3.03(♂ 3.23, ♂ 2.95), 60.93(♂ 63.34, ♂ 60.03) and 5.82(♂ 5.04, ♂ 6.11)% respectively. Lymphocyte count appeared to be higher in the female and in the age group below one year than in the male and in the age group of one year and more, whereas the reverse was the case with neutrophil and monocyte counts. 5. Mean platelet count in the age group of less than one year was 377,391(♂ 398,778, ♂ 368,721)/${\mu}l$, whereas mean platelet count in the age group of one year and more was 354,657 (♂ 373,660, ♂ 347,512)/${\mu}l$. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more($305,513/{\mu}l$) than in the other age groups.

• Korean Journal of Veterinary Research
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• v.27 no.1
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• pp.93-99
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• 1987

A study was conducted to observe the effects of dexamethasone sodium 21 phosphate injection in different amounts, intervals and times on the fluctuation of the parasitized erythrocytes in the peripheral blood of the T. sergenti carrier calves. A total of 31 calves including 15 Holstein and 16 Korean native male calves aged 6-12 months (125-300kg) was used. Results observed were as follows. 1. The calves injected 0.2mg/kg body weight two and three times in 48 hour intervals and 5 times in 24 hour intervals observed that 2 out of 2 heads, 3 out of 6 heads, and 4 out of 5 heads, respectively were substantially increased parasitized erythrocytes in the blood for 3-11 days after the first injection and continued to 2-10 days. 2. However, the calves injected once with 0.1mg/kg body weight and all control calves showed no visible change of the parasitized erythrocytes in the blood during the experimental period. 3. The animals injected over 2 times with 0.2mg/kg body weight tended to be decreased the number of erythrocytes, hemoglobin concentration and PCV for 2-3 days after first injection and continued about one week. 4. All animals injected the dexamethasone were observed the neutrophilic leukocytosis, eosinopenea and lymphopenea until 48 hours after injection.

• Journal of Veterinary Clinics
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• v.23 no.3
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• pp.320-324
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• 2006

The purpose of the present study was to compare pulsatile type(Twin Pulse Life Support; TPLS) with rotary type hemodialyzer(AK95) in order to reduce the dialysis time and to improve dialysis effect. Three healthy dogs(about 30 kg BW) were used. Experimental renal failure was induced by the ligation of bilateral renal artery. A pair of catheters were implanted in jugular vein for dialysis. Daily investigated parameters included clinical signs such as vomiting, fecal appearance and activity, and also laboratory data such as PCV, TP, BUN and creatinine. Hemodialysis was started above 90 mg/100 ml BUN level and, laboratory data were measured every an hour for 4 hours. Heparin was administered 300 IU/Kg before dialysis and 150 IU/Kg via IV route every 90 minutes during dialysis. Clinical signs after induction renal failure were shown severe vomiting, anorexia, diarrhea, mucous feces, ataxia, dilated pupil and episcleral hyperemia. The average of BUN value decreased hourly $99{\pm}12.1,\;84{\pm}12.2,\;72{\pm}8.0,\;58{\pm}7.1,\;48{\pm}5.2,\;and\;39{\pm}3.2mg/100ml$ by hemodialysis. The average of creatinine value decreased $7.8{\pm}0.61,\;6.4{\pm}0.40,\;5.3{\pm}0.42,\;4.5{\pm}0.23,\;4.0{\pm}0.41,\;and\;3.4{\pm}0.42mg/100ml$ according to hemodialysis an hour. There are not significantly differences BUN, creatinine, PCV and TP values between pulsatile and rotary type hemodialysis. These results suggested that effects of hemodialysis with Pulsatile type(TPLS) are not significantly difference as compared with hemodialysis of rotary type(AK95). Further research is needed in order to estimate the influence of cardiovascular and pulmonary system in hemodialysis of pulsatile type.

• Journal of Korean Neurosurgical Society
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• v.54 no.6
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• pp.489-495
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• 2013

Objective : To evaluate the efficacy of temozolomide (TMZ) chemotherapy for recurrent anaplastic oligodendroglioma (AO) and anaplastic oligoastrocytoma (AOA). Methods : A multi-center retrospective trial enrolled seventy-two patients with histologically proven AO/AOA who underwent TMZ chemotherapy for their recurrent tumors from 2006 to 2010. TMZ was administered orally (150 to 200 $mg/m^2/day$) for 5 days per 28 days until unacceptable toxicity occurred or tumor progression was observed. Results : TMZ chemotherapy cycles administered was median 5.3 (range, 1-41). The objective response rate was 24% including 8 cases (11%) of complete response and another 23 patients (32%) were remained as stable disease. Severe side effects (${\geq}$grade 3) occurred only in 9 patients (13%). Progression-free survival (PFS) of all patients was a median 8.0 months (95% confidence interval, 6.0-10.0). The time to recurrence of a year or after was a favorable prognostic factor for PFS (p<0.05). Overall survival (OS) was apparently differed by the patient's histology, as AOA patients survived a median OS of 18.0 months while AO patients did not reach median OS at median follow-up of 11.5 months (range 2.7-65 months). Good performance status of Eastern Cooperative Oncology Group 0 and 1 showed prolonged OS (p<0.01). Conclusion : For recurrent AO/AOA after surgery followed by radiation therapy, TMZ could be recommended as a salvage therapy at the estimated efficacy equal to procarbazine, lomustine, and vincristine (PCV) chemotherapy at first relapse. For patients previously treated with PCV, TMZ is a favorable therapeutic option as 2nd line salvage chemotherapy with an acceptable toxicity rate.

• Journal of Veterinary Clinics
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• v.11 no.1
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• pp.471-478
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• 1994

The hematologic values and serum chemistry were examined healthy male 12 Pere David's Deer and 8 Wapiti at the Farmland Zoological Garden. In wapiti, the blood values collected from horn and jugular vein were compared. The results were as follows: 1. There were not any significant differences in hematologic values and serum chemistry of blood col. looted from horn and jugular vein in wapiti. 2. In Pere David's Deer, Mean$\pm$SD Values of White Blood Cell(WBC) count were 4673.50$\pm$1893.42/${mu}ell$, of Neutrophils 65.42$\pm$12.7%, of Lymphocytes 31.50$\pm$8.68%, of Monocytes 0.25$\pm$0.43%, of Eosinophils 0.17$\pm$0.37%. of Basophils, of Red Blood Cell(RBC) 9.93$\pm$1.38$\times$10/${mu}ell$, of PCV 54.25$\pm$6.25%, of Hb 16.89$\pm$2.43g/㎗, of Tot protein 7.63$\pm$0.6g/㎗, of Cholesterol 87.25$\pm$10.76mg/㎗, of Glucose 109.36$\pm$43.7mg/㎗, of SGOT 85.17$\pm$33IU/$\ell$, of SGPT 102.92$\pm$31.49IU/$\ell$, of BUN 21.00$\pm$5.18mg/㎗, of Creatinine 2.1$\pm$0.43mg/㎗, of ALKP 156$\pm$32.72IU/$\ell$, of Ca 11.28$\pm$1.04mg/㎗, of Na 127.92$\pm$10.04mmo1/$\ell$, of NH3 102.83$\pm$42.25mmo1/$\ell$, of Cl 105.91$\pm$3.45mmo1/$\ell$, respectively. 3. In wapiti, Mean$\pm$SD Values of White Blood Cell(WBC) count were 3450.00$\pm$1040.73/${mu}ell$, of Neutrophils 53.88$\pm$7.42%, of Lymphocytes 36.00$\pm$5.72%, of Monocytes 1.13$\pm$1.05%, of Eosinophils 1.38$\pm$1.93%, of Basophils 7.63$\pm$4.15%, of Red Blood Cell 10.09$\pm$1.3$\times$10/${mu}ell$, of PCV 46.88$\pm$3.33%, of H 15.09$\pm$1.48g/㎗, of Total protein 7.15$\pm$0.73g/㎗, of Cholesterol 78.63$\pm$12.27mg/㎗, of Glucose 75.63$\pm$33.22mg/㎗ , of SGOT 79.25$\pm$18.43IU/$\ell$, of SGPT 36.50$\pm$19.20IU/$\ell$, of BUN 29.63$\pm$4.15mg/㎗, of Creatinine 1.35$\pm$0.21mg/㎗ , of ALKP 283.50$\pm$88.13IU/$\ell$, of Ca 9.78$\pm$0.43mg/㎗ , of Na 120.00$\pm$10.02mmol/$\ell$, of NH3 261.50$\pm$ 103.46mmol/$\ell$, of Cl 92.00$\pm$9.19mmol/$\ell$, respectively.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.151-161
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• 2018

This study was designed to investigate the effects of multi-enzyme on diarrhea and immune responses of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multienzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Frequency of diarrhea, levels of packed cell volume (PCV), white blood cells (WBC), immunoglobulins, cortisol, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), transforming growth $factor-{\beta}$ ($TGF-{\beta}$), and C-reactive protein (CRP) were measured. Multi group tended to decrease (p<0.1) diarrhea frequency than CON group during 2 wk after weaning. Lower values of PCV on d 3 (p<0.05) and d 7 (p<0.1) were found in Multi group compared with CON group. There were no significant differences on WBC number and immunoglobulin (Ig) M and A between Multi and CON groups. However, Multi group tended to increase (p<0.1) Ig G on d 7 than CON group. Moreover, Multi group showed modulated immune responses, indicated by decreased levels of cortisol (p<0.05) on d 7 and 14, $TNF-{\alpha}$ on d 3 (p<0.05) and d 7 (p<0.10), $TGF-{\beta}$ on d 2 (p<0.05) and d 7 (p<0.10), and CRP (p<0.10) on d 3 and 7 after weaning compared with CON group. Consequently, inclusion of multi-enzyme in diets for weaned pigs improved gut health and modulated immune responses of weaned pigs.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.36.1-36.9
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• 2020

Background: Pseudorabies, also known as Aujeszky's disease, is caused by the pseudorabies virus (PRV) and has been recognized as a critical disease affecting the pig industry and a wide range of animals around the world, resulting in great economic losses each year. Shandong province, one of the most vital food animal-breeding regions in China, has a very dense pig population, within which pseudorabies infections were detected in recent years. The data, however, on PRV epidemiology and coinfection rates of PRV with other major swine diseases is sparse. Objectives: This study aimed to investigate the PRV epidemiology in Shandong and analyze the current control measures. Methods: In this study, a total number of 16,457 serum samples and 1,638 tissue samples, which were collected from 362 intensive pig farms (≥ 300 sows/farm) covered all cities in Shandong, were tested by performing enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results: Overall, 52.7% and 91.5% of the serum samples were positive for PRV-gE and -gB, respectively, based on ELISA results. In addition, 15.7% of the tissue samples were PCR positive for PRV. The coinfection rates of PRV with porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus, and classical swine fever virus were measured; coinfection with PCV2 was 35.0%, higher than those of the other two viruses. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Conclusions: The results demonstrate the PRV prevalence and its coinfection rates in Shandong province and indicate that pseudorabies is endemic in pig farms in this region. This study provides epidemiological data that can be useful in the prevention and control of pseudorabies in Shandong, China.