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Choline-Lipid Release from Normal and Transformed Cells

  • Hong, Seong-Tshool;Jang, Yong-Suk;Park, Kie-In
    • BMB Reports
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    • v.29 no.1
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    • pp.73-80
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    • 1996
  • The effect of albumin on phosphatidylcholine (PC) metabolism in Hep-G2, 3T3-H.ras, and 3T3 cells pre-labelled with [Me-$^3H$]choline was studied. The [$^3H$]choline was more efficiently taken up and incorporated into cellular phospholipids in 3T3-H.ras cells than in Hep-G2 and 3T3 cells. In each of the three cell lines, most of the [$^3H$]choline metabolized into the phospholipids was incorporated into PC and only minor was incorporated into lysophosphatidylcholine (LPC). Bovine serum albumin stimulated the release of [$^3H$]LPC and [$^3H$]PC from each of the three cell lines pre-labelled with [$^3H$]choline. [$^3H$]PC was also released in the absence of albumin but [$^3H$]LPC was not. The efficiency of LPC secretion represented as the proportion of medium [$^3H$]LPC to cellular [$^3H$]choline lipid during a chase period is approximately 9 to 14 times greater in 3T3 cells compared with the transformed 3T3-H.ras and Hep-G2 cells. A similar comparison of published data for rat hepatocytes with Hep-G2 shows secretion to be 35~75 times greater from the rat hepatocytes than from Hep-G2. Also, PC secretion from 3T3 cells was 1.6 times more effective than from 3T3-H.ras, whereas rat hepatocytes secrete PC 2.8~3.8 times more effectively than does Hep-G2. The measurement of specific radioactivity of cellular PC in pre-labelled 3T3 cells showed it to be similar to that of the secreted PC. However, the specific radioactivity of secreted LPC was markedly lower than that of the cellular PC, which suggests that LPC is being secreted from a PC pool distinct from that used for PC secretion.

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PC 운영체제의 오늘과 내일

  • 유주진
    • The Magazine of the IEIE
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    • v.19 no.4
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    • pp.1-7
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    • 1992
  • IBM이 16비트 PC를 처음 선보인 81년 이후 오늘에 이르기까지 10여년간의 운영체제(0S) 시장은 마이크로소프트(MS)사의 독주시대로 요약할 수 있다. 그러나 지금까지 이렇다 할 변화를 보이지 않던 OS제품은 90년대에 들어서면서 커다란 변화의 조짐에 횝싸이고 있다. 그동안 8086, 80286등의 마이크로프로세서를 탑재한 16비트 PC시장이 80386,80486등을 탑재한 32비트 시장으로 급변하기 시작했고 종전 데스크탑 일변도의 PC시장은 랩톱,노트북형 등의 휴대형컴퓨터와 펜컴퓨터,멀티미디어 등의 차세대 제품등으로 세분화되기 시작한 것이다. 또 32비트 시대가 다가오면서 한사람이 한 대의 PC로 일을 하는 종전 PC운용환경은 넷워킹과 멀티테스킹이 강조되는 다중작업 환경으로 전환되고 있으며 윈도즈(Windows) 3.0의 대히트로 IBM PC에서도 GUI(그래픽 사용자인터페이스) 환경을 요구, 이를 위한 새로운 05의 등장이 불가피해지고 있다. 게다가 지금까지 메인프레임을 중심으로 한 중앙집중방식의 컴퓨터환경이 다운사이징화 되면서 넷워크 환경을 기반으로 한 PC의 역할이 크게 강조, 이를 위한 운영체제 또한 새로운 영역으로 대두되고 있다. 불과 1∼2년 사이에 급진전되고 있는 이같은 변화의 물결은 필연적으로 다양한 운영체제의 개발을 가져왔고 이를 통해 차세대 PC시장을 주도하기 위한 업계의 패권다툼은 전쟁을 방불케할 만큼 치열해지고 있다. MS사의 전유물이었던 DOS 영역에서는 최근 노벨사와의 합병으로 전열을 가 다듬은 디지틀리서치사가 가세, 한판승부를 선언하고 나섰으며 고성능 PC시대의 패권을 잡기 위해 10년지기였던 IBM과 MS사는 각각 OS/2와 윈도즈를 내세우고 양보할 수 없는 힘겨루기에 들어갔다. 또 이들 양사는 펜컴퓨터,멀티미디어등 차세대제품의 운영체제 시장을 둘러싸고 일찍부터 격전에 들어갔으며 IBM과 MS사의 혼전을 틈타 썬마이크로시스템을 필두로한 워크스테이션 업체 및 유닉스진영까지 고성능 PC시장을 겨냥한 OS를 속속 개발, 90년대의 OS 전쟁은 한치 앞을 내다볼 수 없는 안개국면으로 접어들고 있다. DOS에서 32비트시대,펜컴퓨터, 멀티미디어에 이르는 차세대제품을 둘러싼 업계의 OS 쟁탈전을 통해 OS의 발전동향과 미래를 전망해 본다.

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Seasonal Growth Inhibition of Perennial Ryegrass (Lolium perenne L.) after Applying Two Plant Growth Regulators (생장조정제 처리 후 생육 시기별 퍼레니얼 라이그래스의 억제 효과)

  • Seung Jae Lim;Tae Wooung Kim;Young-Sun Kim
    • Journal of Bio-Environment Control
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    • v.32 no.3
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    • pp.242-248
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    • 2023
  • This study was conducted to evaluate the effects of trinexapac-ethyl (TE) and prohexadione-calcium (PC) on the growth and turf quality of perennial ryegrass (Lolium perenne L.). Treatments were designed as follows; control (non-treatment), TE (TE 0.01 a.i. g·m-2·100mL-1), and PC (PC 0.01 a.i. g·m-2·100mL-1). Compared with control, turf color index and chlorophyll content of TE and PC treatments were not significantly different. As applied plant growth regulators (PGRs), shoot length in the TE and PC was decreased by 8.9-12.4% and 6.7-13.6%, respectively, and clipping yield by 44.4-45.8% and 40.6-40.9%. When evaluated with the growth of shoot length after applying PGRs, residual days of TE and PC were 43.6 and 37.9 days after treatment of PGRs (DAT) in the 1st experiment (July 29-August 26), respectively, and 38.3 and 39.5 DATs in the 2nd experiment (September 30-October 28). These results indicated that an application of TE or PC decreased shoot length and clipping yield in the perennial ryegrass, and their residual time was about 40±3 DATs. Perennial ryegrass applied with PGRs like TE and PC was not significant seasonal difference, and might be not phytotoxic.

디지털저작권보호와 강인한 영상 워터마킹 기술

  • 송학현;김윤호
    • The Magazine of Kiice
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    • v.5 no.1
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    • pp.38-48
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    • 2004
  • 정보기술(Information Technology ; IT)의 발전을 대표하는 상징 중에 하나는 것은 각 가정에 설치된 개인용 컴퓨터(Personal Computer)를 들 수 있다. 즉, PC의 보급이 광범위해지면서 PC가 생산, 보유한 정보를 서로 공유하고 교환할 필요성을 느끼게 되어 특정 개인의 PC에만 국한되지 않고 networked PC가 필요하게 되었고, PC간의 데이터통신을 위한 다양한 기술들이 개발되기 시작하였다. 그 첫 번째 시도가 지리적으로 가까이 있는 컴퓨터간의 통신네트워크를 구성하려는 시도였는데, 이것이 근거리 통신망(LAN)과 1990년대 중반에 탄생한 웹(WWW; world wide web)의 출현이다. (중략)

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A Study on Improvement of Valuable Metals Leaching and Distribution Characteristics on Waste PCBs(Printed Circuit Boards) by Using Pulverization Process (폐 PCBs의 미분쇄 공정 적용에 따른 유가금속 분포 특성 및 금속 침출 향상에 관한 연구)

  • Han, Young-Rip;Choi, Young-Ik
    • Journal of Environmental Science International
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    • v.24 no.2
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    • pp.245-251
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    • 2015
  • The main objective of this study is to recovery valuable metals with metal particle size distributions in waste cell phone PCBs(Printed Circuit Boards) by means of pulverization and nitric acid process. The particle size classifier also was evaluated by specific metal contents. The PCBs were pulverized by a fine pulverizer. The particle sizes were classified by 5 different sizes which were PcS1(0.2 mm below), PcS2(0.20~0.51 mm), PcS3(0.51~1.09 mm), PcS4(1.09~2.00 mm) and PcS5(2.00 mm above). Non-magnetic metals in the grinding particles were separated by a hand magnetic. And then, Cu, Co and Ni were separated by 3M nitric acid. Particle diameter of PCBs were 0.388~0.402 mm after the fine pulverizer. The sorting coefficient were 0.403~0.481. The highest metal content in PcS1. And the bigger particle diameter, the lower the valuable metals exist. The recovery rate of the valuable metals increases in smaller particle diameter with same leaching conditions. For further work, it could improve to recovery of the valuable metals effectively by means of individual treatment, multistage leaching and different leaching solvents.

External Morphology and Numerical Taxonomy among Habitat of Allium victorialis var. platyphyllum (산마늘의 자생지별 외부형태 및 수리분류학적 연구)

  • 유기억
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.210-216
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    • 1998
  • Taxonomic studies in external morphology, principal component analysis and cluster analysis were conducted to understand the intraspecific relationship among three habitats (Jirisan, Odaesan and Ulleungdo) of allium voctorialis var. platyphyllum. External morphology such as bulb color, leaf blade length and width, petiole length, total leaf length, peduncle length, perianth lobe length and width, length of anther and filament were useful characters for identification of poplations in three habitats. The results obtained based on the principal component (Pc) analysis of treated 72 OTUs(included outgroup) were divided into three groups by the PC 1 ,2,3 and the sums of contributions for the total variance were 84.1%(PC1 51.0%, PC2 24.9% and PC3 8.2%, respectively). In cluster analysis by the UPGMA and Ward's methods , there was similarities in the compostion of clustered taxa, and only Ulleungdo population was distinctly identified from population of other two habitats.

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Photochemical and Thermal Solvolysis of Picolyl Chlorides

  • Shim Sang Chul;Choi Seung Ju
    • Bulletin of the Korean Chemical Society
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    • v.3 no.1
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    • pp.30-33
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    • 1982
  • Photochemical and thermal solvolysis of 2,3,4-picolyl chlorides (2,3,4-PC) were studied in amine solvents and the results were correlated with the electronic structures calculated by PPP-SCF-MO CI method. Activation parameters show that the thermal solvolysis of PC is $S_N2$ type rcaction. The rates of thermal reaction in pyridine or t-butylamine solvent decrease in the order of 2-PC > 3-PC > 4-PC. These results are consistent with the predictions based on the electron densities of picolyl chlorides. In photosolvolysis, the same products as those of thermal reactions were obtained. The results indicate that photochemical solvolysis undergoes through heterolytic cleavage. Relative quantum yields of photosolvolysis of 2,3,4-picolyl chlorides in t-butylamine solvent were determined to be 0.73, 1, and 0.50 respectively. These results are in good agreement with the electron densities of the excited triplet state of picolyl chlorides.

Value of KRAS, BRAF, and PIK3CA Mutations and Survival Benefit from Systemic Chemotherapy in Colorectal Peritoneal Carcinomatosis

  • Sasaki, Yusuke;Hamaguchi, Tetsuya;Yamada, Yasuhide;Takahashi, Naoki;Shoji, Hirokazu;Honma, Yoshitaka;Iwasa, Satoru;Okita, Natsuko;Takashima, Atsuo;Kato, Ken;Nagai, Yushi;Taniguchi, Hirokazu;Boku, Narikazu;Ushijima, Toshikazu;Shimada, Yasuhiro
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.2
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    • pp.539-543
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    • 2016
  • Background: It is well known that peritoneal carcinomatosis (PC) from colorectal cancer (CRC) is associated with a poor prognosis. However, data on the prognostic significance of modern chemotherapy containing bevacizumab, cetuximab or panitumumab are not available. Materials and Methods: This retrospective review concerned 526 patients with metastatic CRC who were classified into two groups according to the presence or absence of PC, and were treated with systemic chemotherapy, with or without bevacizumab or anti-EGFR antibodies. The genetic background, in particular KRAS, BRAF, and PIK3CA gene mutations, and overall survival (OS) were compared between the two groups. Results: The median OS values were 23.3 and 29.1 months for PC and non-PC patients, respectively (hazard ratio [HR]=1.20; p=0.17). Among all patients, tumor location, number of metastatic sites and BRAF mutation status were significant prognostic factors, whereas the presence of PC was not. In the PC group, chemotherapy with bevacizumab resulted in a significantly longer OS than forchemotherapy without bevacizumab (HR=0.38, p<0.01), but this was not the case in the non-PC group (HR=0.80, p=0.10). Furthermore, the incidence of the BRAF V600E mutation was significantly higher in PC than in non-PC patients (27.7% versus 7.3%, p<0.01). BRAF mutations displayed a strong correlation with shorter OS in non-PC (HR=2.26), but not PC patients (HR=1.04). Conclusions: Systemic chemotherapy, especially when combined with bevacizumab, improved survival in patients with PC from CRC as well as non-PC patients. While BRAF mutation demonstrated a high frequency in PC patients, but it was not associated with prognosis.

A Study on the Exposure to PC Pornography and the Post-Exposure Reactions of Middle School Boys (남자중학생의 컴퓨터음란물 접촉실태와 접촉반응에 대한 조사연구)

  • Kim, Y.-H.;Lee, H.-Z.;Jung, H.-M.
    • The Journal of Korean Academic Society of Nursing Education
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    • v.6 no.1
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    • pp.7-22
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    • 2000
  • The purpose of this study was to identified the level of the exposure to the PC Pornography and what's their post-exposure reactions to the PC pornography. The subjects were 423 middle school boys in Pusan. The survey was executed from July 12 through October 30, 1999. The collected data were analyzed by SPSS WIN using the %, mean, ANOVA. The results of this study were as follows: 1. 79.4%(336 boys) were exposed to the PC pornography. 46.3%(196 boys) answered that they experienced the published pornography prior to the PC pornography. 2. 78.5%(333 boys) answered that they had their PC in their homes. This ratio revealed the higher level of possession. 67.6% said that their PC were set in their own rooms. 57.9% of the subject answered they experienced the Internet Communications. 68.6% of the subject said that they tried PC communications once or twice in a week. 38.5% of the subject expressed that they used their own PC for 1-2 hours in a day. Most of them(82.7%) enjoyed the PC games or entertainment programs. 81.3% of the subject answered that their school performance didn't show the progress after the use of their own PC. 78.3% of the subject answered that they acquired information. 45.2% of the subject answered that they made their friend through the Internet communications. 78.7% of the subject answered that the time of family dialogue decreased. 70.7% of the subject answered that the time of their TV watching decreased. 3. As for their post-exposure reactions the subject who were exposed to the PC Pornography showed the point 2.93 in the item 'pleasure'. The point of 'sexual impulses' was 2.81. But the point of 'anxiety' was 2.75, the point of 'dislike or disgust' was 2.62, the point of 'guilty' was 2.59. 4. According to the frequency, the motivation and the companionship on the exposure to PC Pornography, their post-exposure reaction scores showed differences significantly. Finally, the post-exposure reaction had relation to the frequency, the motivation, the companionship and the place (F=3.28 ~ 8.40, p=.012 ~ .000).

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Preparations and Antistatic/UV Blocking Properties of Dual Functional Phthalocyanine Materials (기능성 프탈로시아닌 물질의 제조 및 대전방지/UV 차단 특성)

  • Kang, Young-Goo;Ihm, Dae-Woo;Kim, Shi-Surk;Park, Byoung-Ki
    • Journal of the Korean Society of Safety
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    • v.23 no.1
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    • pp.66-71
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    • 2008
  • A new route to phthalocyanine complexes were developed to synthesize these products by fusion in the absence of solvent. This new method of synthesis without using solvent has advantages over the conventional synthetic methods since there are no risk of explosion and formation of harmful vapor from organic solvent. Reaction of PcFe with axial ligands such as $PcFe(4-VP)_2$[Pc: Phthalocyanine, 4-VP: 4-Vinylpyridine] and $PcFe(VIM)_2$[VIM: 1-Vinylimidazole] afforded powderlike, pure dark greenish blue colored products. The resulted products are soluble in $CH_2Cl_2$ and found to be complexes of the type $PcFeL_2$. Spectral properties were studied with ATR-FTIR and UV/Vis. Thermal and electrical characterization was also performed. Phthalocyanine complexes exhibit useful properties such as UV/Vis blocking, antistatic characteristics and excellent thermal stability and we anticipate various applicability in numerous products.