• 미생물학회지
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• 제36권4호
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• pp.249-253
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• 2000

Cyanobacteria의 광합성 보조색소인 phycocyanin의 PC operon(cpc gene)을 PCR로 증폭하고, 제한효소로 처리하여 RFLP pattern을 비교하였다. Intergenic spacer sequence를 포함한 cpc gene은 실험에 사용한 cyanobacteria 균주 모두에게 증폭되었으며, 산물의 size는 약 700 bp였다. PCR산물을 5종의 제한효소로 처리한 결과 AluI, MspI, HaeIII는 같은 속내으ㅐ 균주간에 동일한 pattern을 나타내어 속 구분이 가능하였으며 CfoI은 Anabeana와 Synechocystis속의 균주간에 구별되는 양상을 나타내어 속내 균주 구별에 유용하였다. Restriction enzyme profile에 의한 phenogram에서 Anabeana, Chlorogloea, Synechyhocystis는 각각 하나의 cluster를 형성하여 cyanobacteria의 분류에 PC-IGS의 RFLP pattern이 유용함을 알 수 있었다.

• 한국환경과학회지
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• 제14권7호
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• pp.649-655
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• 2005

Microcystis aeruginosa is common form of cyanobacteria (blue-green algae) capable of producing toxic heptapeptide (microcystin) that cause illness or death. The comparison of molecular genetic method with the morphological characteristics of cyanobacteria was conducted. We have designed PCR primers (JJM98F, JJM1141R) for cyanobacterial 16S rRNA and phycocyanin intergenic spacer (PC-IGS) gene domain. To confirm the production of microcystins, PCR primers for the N-methyltransferase (NMT) domain of microcystin synthetase gene mcyA were designed using 21 cyanobacteria strains Most of isolated strains from the Nakdong River was classified as Microcystis aeruginosa and the similarities were $99\%$ with M. aeruginosa AF 139292. $38.1\%$ of isolated strains contained microcystin synthesis gene. NMT (N-methyltransferase) were not detected in isolated strain in several strains, which means non-toxic. However, the NMTs of the strains were detected during the cultivation.