• Korean Journal of Poultry Science
• /
• v.40 no.3
• /
• pp.271-276
• /
• 2013

A study was conducted to investigate the effect of dietary supplementation of feedstuff of Chlorella (Chlorella vulgaris) to replace of antibiotic in the diets of broiler chickens. A total of 720 1-d-old straight run broiler chicks (Ross ${\times}$ Ross) was randomly assigned into six treatments with four replicate pens (30 birds/replicate pen) for 5-wk. A corn-soy bean meal basal diet was formulated, the treatment groups were negative group (NC, antibiotic-free diet) and 0.1% virginiamycin in as antibiotic growth promoters (PC), 1.0% fresh liquid Chlorella (T1), 1.0% dried Chlorella powder (T2), 1.0% commercial Chlorella product and 1.0% (T3) and commercial Chlorella product 0.5% (T4) were added to the basal diet to form six dietary treatments. No significant differences were found among the treatments for feed intake and feed conversion of broiler chickens during the whole experimental period, but the BW gain was significantly higher (P<0.05) in commercial Chlorella product supplemental groups than the control group (NC and PC groups). Dietary supplementation of Chlorella significantly (P<0.05) increased the plasma IgA, IgM and IgG concentration of chicks compared to NC and PC groups. Supplemental AGPs and commercial chlorella product did not affect the E. coli and Salmonella concentration in the intestinal microflora of broiler chicks; however, the population of Lactobacillus was significantly increased (P<0.05) when birds were fed commercial Chlorella product groups. It is concluded that commercial Chlorella product supplementation could be used as an alternative of antibiotics to promote growth and immune response by increasing the production of lactic acid bacteria in the intestinal microflora of broiler chickens.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.7 no.5
• /
• pp.94-99
• /
• 2004

This study was carried out to find out what is the optimum conditions for RAPD of Zelkova serata. We changes the factors what affect to PCR band patterns, as a result, we established the optimum conditions as follows; template DNA 100mg, Primer 0.25uM, dNTP 100mM, Taq polymerase 1.0u, and total reaction volume was filled up to 10uL with distilled water. As the amount of primers went higher, PCR reaction rates were lowered. This reason was cause by exhaustion of primers during initial reaction. The amount of dNTP didn't showed noticable differtations between the range, but the optimum amount was 100mM for efficiency. Taq polymerase 1.0 unit was the best in the range. As the concentration of polymerase were increased, many non-specific bands were appeared, In primer selection, most Openron Random Primers are amplified in this experiment. The primers GC contents were 60, and set A, B, C, D, E, X were tested. Thermal cycler(ASTEC PC808, Japan) condition was, $95^{\circ}C$, 5min, initial denaturation, $94^{\circ}C$, 20sec, denaturation, $37^{\circ}C$, 40sec, annealing, $72^{\circ}C$, 1min, extention, 45cycle repeated and final extention $72^{\circ}C$10min.

• Journal of Oriental Neuropsychiatry
• /
• v.19 no.3
• /
• pp.117-127
• /
• 2008

Objective: Bee venom (BV) has been used for the treatment of inflammatory diseases such as rheumatoid arthritis and relief of pain in Oriental medicine. The two main components of BV are melittin and phospholipase A2 (PLA2). Of these, melittin, the major active ingredient of BV, has been reported to induce apoptosis and to possess anti tumor effects. Several studies have established that the agents inducing apoptosis in target organs suppress tumorigenesis. As the other component, PLA2 has been reported to induce neurite outgrowth in PC12 cells. However, there was no report about proliferative effect of BV in neuronal cells. In order to examine the effect of BV on glioma cell, human glioma cell line, U87 was used. Methods: Analysis of proliferation was confirmed by MTT assay. BV increased cell number through dose and duration dependent manner and these effects are apparent at a concentration of 10 ug/ml. To observe which signaling molecules will be activated by BV, phosphorylation of Akt, MAPK, PYK2 or CREB were examined by Western blot analysis. To study the long term effect of BV in U87 cells, the image of cells treated with BV for 4 days were obtained. Results: The phosphorylation levels of PYK2 and Akt were increased at 5 min after addition of 10 ug/ml of BV and sustained to 2 hours. On the other hand, phosphorylation of MAPK and CREB were increased at 5 min, maximum at 10 min, and returned to 30 min. These imply that BV may activate two different signaling pathways, PYK2/Akt and MAPK/CREB. BV treated cells showed increased neurite number and length. Conclusion: These results propose that BV may induce differentiation as well as proliferation of U87 cells through the activation of PYK2/ Akt and MAPK/ CREB.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.65 no.10
• /
• pp.1755-1760
• /
• 2016

Automation of greenhouses has proved to be extremely helpful in maximizing crop yields and minimizing labor costs. The optimum conditions for cultivating plants are regularly maintained by the use of programmed sensors and actuators with constant monitoring of the system. In this paper, we have designed a prototype of a smart greenhouse using Arduino microcontroller, simple yet improved in feedbacks and algorithms. Only three important microclimatic parameters namely moisture level, temperature and light are taken into consideration for the design of the system. Signals acquired from the sensors are first isolated and filtered to reduce noise before it is processed by Arduino. With the help of LabVIEW program, Time domain analysis and Fast Fourier Transform (FFT) of the acquired signals are done to analyze the waveform. Especially, for smoothing the outlying data digitally, Moving average algorithm is designed. With the implement of this algorithm, variations in the sensed data which could occur from rapidly changing environment or imprecise sensors, could be largely smoothed and stable output could be created. Also, actuators are controlled with constant feedbacks to ensure desired conditions are always met. Lastly, data is constantly acquired by the use of Data Acquisition Hardware and can be viewed through PC or Smart devices for monitoring purposes.

• Proceedings of the KIEE Conference
• /
• 2009.07a
• /
• pp.1461_1462
• /
• 2009

송전선로에 설취된 애자들은 지속적인 전기적 기계적 스트레스에 노출되어 있기 때문에 여러 환경적 요인으로 인해 애자 표면의 열화가 가속된다. 이와 같은 애자의 표면 열화는 dryband를 형성하게 되고 지속적인 애자 표면상의 dryband와 누설 전류 증가의 상호작용은 애자의 섬락를 일으키는 원인이 된다. 이러한 연구의 결과 고전압용 애자로 유입되는 누설 전류는 애자 열화의 정도를 평가할 수 있는 매우 중요한 parameter임을 알 수 있다. 따라서 고전압용 애자의 누설전류를 분석하고 취득하여 애자의 열화 평가 및 이로 인해 발생할 수 있는 결함 애자의 검출에 대한 연구가 절실히 요구되고 있다. 본 논문에서는 고전압용 애자 중 자기애자의 습도변화에 따른 누설전류 특성을 조사하기 위해 자체 제작한 실험 챔버, power transformer, 전류센서, 오실로스코프, PC등으로 구성된 실험 시스템을 구축하였고, 데이터 분석을 위한 S/W로서 LabView를 사용하였다. 그에 따른 실험결과로서 다양한 습도변화(30%~90%)의 경우 정상 애자련과 결함 애자를 포함한 애자련의 누설전류 데이터를 취득하여 본 연구에서 제시한 알고리즘을 통해 분석함으로써 정상 애자 내의 결함 애자 포함 여부를 판별하고 다양한 습도 변화시에 정상 애자련과 불량 애자련의 특징을 볼 수 있었다.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.28 no.9A
• /
• pp.746-753
• /
• 2003

In this paper, we propose an efficient algorithm for non-streaming synchronized data processing based on ATSC-DASE in terrestrial digital data broadcasting services. Non-streaming synchronized data is encapsulated in DSM-CC sections with PTS(presentation time stamp) values associated with A/V and it is transmitted in a form of MPEG-2 TS(transport stream). At the receiver, the transmitted A/V data are processed by PC based set-top box(STB) in real-time, and the transmitted non-streaming synchronized data is also stored at the STB and is displayed at right time by the proposed algorithm. To verity the proper operation of the proposed algorithm, we make a scenario for non-streaming synchronized data by XML, and finally we are able to display it properly by using declarative application(DA) browser.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2007.09a
• /
• pp.623-634
• /
• 2007

The aim of this research is development and verification of resistivity seismic dilatometer (RSDMT) system. The resistivity module for obtaining resistivity-depth plot and seismic module for obtaining wave velocity-depth plot are attached to the conventional flat dilatometer testing equipment. To enhance reliability and repeatability of seismic part in RSDMT, automatic testing system including automatic surface source, PC based data acquisition system and operating program were developed. To obtain real resistivity value of soil, geometric factor for the array of electrodes in RSDMT was derived empirically. The verification studies for the developed RSDMT system were performed at the southeast side of Korea where soil improvement work is planned. SPT, CPT, geophysical subsurface imaging techniques and some laboratory tests were performed for the comparisons. As one penetration of RSDMT, various soil parameters could be obtained. The results of field test showed good repeatability and reliability in every part. From these studies, developed RSDMT system was checked and the effectiveness of this system was verified in light of proper evaluation of geotechnical characteristics of soft soil.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.4 no.4
• /
• pp.71-88
• /
• 2009

The Korean online gaming industry has seen rapid growth since the boom of information technology (IT) related industries, especially Internet and PC-bang or local area network (LAN) use, throughout the country. Online games are real-time games in which two or more players meet, compete, and cooperate in the same game space using the same communication network. Even though the growth rates of IT-related industries have recently slowed, the growth of the online gaming industry has increased, and Korean firms are expanding their businesses to countries all over the world. However, the online gaming industry is becoming an oligopoly, in which 4-5 leading companies occupy most online gaming markets. This situation presents a disadvantage to the Korean online gaming industry, since online players usually seek a diversity of online gaming contents and high quality online game services. Therefore, small- and medium-sized game developers must survive in this market by upgrading the quality of their online gaming services in an effort to provide differentiated goods and services in monopolistic competition markets. However, most venture companies in the online game industry, whether new or previously existing, are not able to obtain adequate financing. The objective of this paper is to examine the management environment for medium- and small-sized game developers in the Korean online gaming industry. By introducing the case of Grigon Entertainment, we try to identify the source of difficulty obtaining external financing for Korean venture firms in the online gaming industry.

• YAKHAK HOEJI
• /
• v.55 no.6
• /
• pp.510-515
• /
• 2011

Isoquinoline compounds including berberine enhance L-DOPA-induced cytotoxicity in PC12 cells. In this study, the effects of berberine on L-DOPA therapy in unilateral 6-hydroxydopamine (6-OHDA)-induced rat models of parkinsonism were investigated. Rats were prepared for the models of Parkinson's disease by 6-OHDA-lesioning for 14 days and then treated with L-DOPA (10 mg/kg) with or without berberine (5 and 30 mg/kg, i.p.) for 21 days. Treatment with berberine (5 and 30 mg/kg, i.p.) showed a dopaminergic cell loss in substantia nigra of 6-OHDA-lesioned rats treated with L-DOPA: 30 mg/kg berberine was more intensive neurotoxic. The levels of dopamine were also decreased by berberine (5 and 30 mg/ kg) in striatum-substantia nigra of 6-OHDA-lesioned rats treated with L-DOPA. These results suggest that berberine aggravates cell death of dopaminergic neurons in L-DOPA-treated 6-OHDA-lesioned rat models of Parkinson's disease. Therefore, the long-term L-DOPA therapeutic patients with isoquinoline compounds including berberine may need to be checked for the adverse symptoms.

• Korean Journal of Materials Research
• /
• v.12 no.7
• /
• pp.587-590
• /
• 2002

A silver indium sulfide ($AgInS_2$) epilayer was grown by the hot wall epitaxy method, which has not been reported in the literature. The grown $AgInS_2$ epilayer has found to be a chalcopyrite structure and evaluated to be high quality crystal. From the photocurrent measurement in the temperature range from 30 K to 300 K, the two peaks of A and B were only observed, whereas the three peaks of A, B, and C were seen in the PC spectrum of 10 K. These peaks are ascribed to the band-to-band transition. The valence band splitting of $AgInS_2$ was investigated by means of the photocurrent measurement. The crystal field splitting, $\Delta_{cr}$ , and the spin orbit splitting, $\Delta_{so}$ , have been obtained to be 0.150 eV and 0.009 eV at 10 K, respectively. And, the energy band gap at room temperature has been determined to be 1.868 eV. Also, the temperature dependence of the energy band gap, $E_{g}$(T), was determined.d.