• 정보화사회
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• s.123
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• pp.70-71
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• 1998

정보통신부는 정보화의 진전에 따라 증가하고 있는 전자문서 및 인터넷 전자상거래의 안정성.신뢰성 확보를 위하여 전자서명법(안)을 마련. 그동안 산.학.연등 관계전문가로 작업반을 구성, 7차에 걸친 작업회의를 통해 마련된 이 법(안)은 98년 정기국회 제출 목표로 5월부터 관계부처 및 PC통신을 이용한 전자공청회 등 여론수렴에 들어갔다. 그러나 구체적인 입법시기는 UN국제상거래법위원회의 전자서명 통일규격 제정작업 등 해외동향을 고려햐여 신중히 추진해 나갈 계획이다.

• Journal of the Korea Concrete Institute
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• v.22 no.3
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• pp.419-426
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• 2010

This paper discusses a series of experiments including material improvement in order to ensure quality of grouting for the post-tensioned structure. In presstressed concrete, grouting refers to the construction procedure of filling empty space of duct enclosing the prestress tendons using cementitious material, To date, adequate quality control of the grouting has not been established in Korea because the relationship between the grouting and durability of post-tensioned structure is not well-recognized. The Korean standard does not consider the important material characteristic, wick effect, which is caused by tendons in the ducts, and furthermore current standard testing method does not quantify reasonable material segregation. As a result, the grout material, which satisfies the current material standards, may well exhibit excessive bleeding of water or shrinkage during construction. In this study, international codes and standards related to grouting were surveyed. The mix proportions of the constituents and novel admixtures were suggested to meet equivalently with these standards. Performance of this enhanced grout was compared with common domestic grouts using the international standard testing method. A series of mock-up specimens considering geometry of PC beam was constructed and grout flow pattern was observed as the grout was injected. It was observed that the grouting performance was highly influenced by material properties and filling characteristic can be varied depending on geometry of ducts.

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.181-186
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• 2003

This study was performed to analyze quantitatively the number of living bacteria in forest soil samples collected from Mt. Keryong using improved direct viable count (DVC) and plate count (PC) methods. The number of living bacteria by DVC comprised 18～44% of the total direct count (TDC), whereas the number of living bacteria by PC was less than 1% of TDC. These results showed that viable but non-culturable (VBNC) bacteria existed in the soil with high percentages. Besides, DVC was proved to make it possible to make a quantitative detection of the VBNC bacteria. On the other hand, upon measuring the value from the conventional nutrient broth (NB) and $10^{-2}$ folded diluted nutrient broth (DNB), the values from the DNB showed 5 to 10 times higher than those from the conventional NB medium. These results indicate that oligotrophic bacterial groups, which could multiply in the low nutrient broth, abundantly exist in the soil ecosystem. It would also be possible to apply this kind of method to other substrate to make a quantitative detection of soil bacterial groups.

• Journal of Korean Tunnelling and Underground Space Association
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• v.25 no.2
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• pp.43-63
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• 2023

With the concentration of the population in the city center and the saturation of the structures on the ground, the development of the underground structures becomes important and the construction of an adjoining tunnel that can reduce the overall problems is respected. In addition, it is necessary to apply the reinforcement construction method for the pillar part of the adjacent tunnel that can secure stability, economy and workability of the site. In this study, the tunnel pillar reinforcement method using prestress and grouting was reviewed. There are various reinforcement methods that can compensate for the problems of the side tunnel, but as the tunnel pillar construction method using prestress and grouting is judged to be excellent in field applicability, stability and economic feasibility, theoretical and numerical analysis of the actual behavior mechanism are conducted. Numerical analysis is divided into PC stranded wire + steel pipe reinforcement grouting + prestress (Case 1), pillar part tie bolt reinforcement (Case 2), pillar part non-reinforcement (Case 3) under the same ground conditions, and the maximum value of the celling displacement, internal displacement, and member force, the stability was confirmed. Through numerical analysis, it was confirmed that Case 1 which reinforced the PC stranded wire, was the best construction method and if it is verified and supplemented through field experiments later, it will be possible to derive superior results in terms of displacement control and member force than the currently applied reinforcement method was judged.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1994.04a
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• pp.434-437
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• 1994

기계부품의 설계공정에 3차원 CAD 시스템이사용되어 널리 보급중에 있으며, 그중에서도 시설설계 의 경우 CAD형상 데이터의 급속입체표현법, 즉 3차원 입체모형에 의한 프로토타입 모델의 표현기술 개발에 관한 연구개발이 현재 세계적으로 널리 진행되고 있다. 이 연구개발의 첫단계는 3차원 CAD 의 형상데이터를 어떻게 처리하여 실제 원하는 형상을 가공해낼 것인가를 해결하는 것으로 3차원 CAD의 변환파일중 한가지안 STI 파일을 이용하여 이것의 해결방법에 대해 논한다. 본 연구에서 사용한 3차원 CAD는 Pro-Engineer이며, 이것을 PC로 down load 받아서 PC 상에서의 형상이입작업은 AutoCAD를 사용하였다.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2007.04a
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• pp.35-36
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• 2007

고분자는 다양한 산업 분야에 응용되고 있으며, 표면의 기능화 처리로 그 응용 분야를 넓히는 것이 가능하다. 본 연구에서는 고분자 (PC, PET) 표면에 N - (2 - aminoethyl) 3 -aminopropyl trimethoxysilane (AEAPS) 분자막을 Thermal CVD법에 의해 형성시키고, XPS와 수적접 촉각을 이용하여 표면 상태를 측정하였다. 또한, AEAPS 분자막에 Carboxy acid과 Pd 이온의 흡착 실험을 통해 고분자 표면에서의 amino기 작용에 대한 연구를 하였다.

• Journal of Plant Biology
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• v.36 no.4
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• pp.415-423
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• 1993

대두의 uricase II cDNA를 탐침으로 plaque 혼성화 방법에 의해 해녀콩의 뿌리를 cDNA library로부터의 두 개의 phage 클론(λCINUO-01, λCINUO-02)을 선별하였다. 두 phage 클론은 약 1.6 kb와 1.0 kb의 insert를 갖고 있었으며 이들의 염기서열을 결정하기 위하여 pUC19과 pBSKS vector에 subcloing(pcCLNUO-01, pcCLNUO-02)하였다. Sanger법에 의해 염기서열을 결정한 결과, 두 클론은 각각 1,611 bp와 1,024 bp로 이루어져 있었으며 pcCINUO-01은 308개의 아미노산, pcCINUO-02는 301개의 아미노산을 암호화하는 open reading frame(ORF)을 갖고 있었다. 두 클론의 ORF의 염기서열은 대두의 uricase II와 각각 88.9%, 89.3%의 상동성을 보여주었으며, 아미노산 서열은 84.1%, 85.4%의 상동성을 보여주었다. pcCINUO-01의 경우, 종결코돈으로부터 313 NT 하류쪽에 진핵생물의 poly(A) 첨가신호인 AATAAA 서열이 존재하였으며 이로부터 21 NT 하류쪽에 17 잔기의 poly(A)가 존재하였다. 두 클론의 염기서열에서 추정된 아미노산 서열의 카르복시 말단에는 세포질에서 합성된 몇몇 단백질들이 peroxisome으로 수송되는데 필요한 신호서열인 Ser-Lys-Leu-COOH 서열이 존재하고 있었다. 두 클론의 염기서열을 토대로 아미노산 조성을 살펴본 결과, 염기성 아미노산(Arg, His, Lys)과 산성 아미노산(Asp, Glu)이 각각 46 대 35, 47 대 35의 비를 보여주었는데 이는 uricase II 단백질의 염기성 성질을 보여주는 결과로 추정된다. Northern 혼성화 결과 해녀콩에서 uricase II는 뿌리혹에서만 특이적으로 발현됨을 알 수 있었고 게놈 혼성화 반응 결과는 uricase II 유전자가 해녀콩 게놈상에 유전자 가족으로는 존재할 수 있음을 보여주었다.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.5
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• pp.533-540
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• 2008

In this study, a new slab system where a part of precast slab is connected each other by viscoelastic material is proposed and numerical analysis is performed to evaluate the effect of the connection between the material and PC slab on the vibration and noise control. Substructuring is introduced to develop the equation of motion for the slab system. In addition, the optimal properties of viscoelastic material are investigated. For the performance evaluation of the new slab system, the sound power and acceleration responses of the slab are compared with those of two way slab and one way slab, respectively. Numerical analysis results show that the sound power of the new slab system can be reduced by viscoelastic material significantly.

• KSBB Journal
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• v.18 no.3
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• pp.229-233
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• 2003

Antioxidant effect of reconstituted liposome was studied for application to food and cosmetic industry. Vitamin-C/alginate alginate gel-entrapped DHA-PC liposome (AVDL) was prepared and studied with respect to morphology (TEM), loading efficiency (TLC) and antioxidant effect (TBARS assay). AVDL has spherical structure and 80 ∼ 250 ㎚ size. As DHA content increased, DHA and PC loading efficiency and antioxidant effect was increased. At addition of 0.2 % vitamin C in AVDL, DHA auto-oxidation was minimized and sustained about 3 days.

• Journal of the Korean Institute of Gas
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• v.13 no.1
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• pp.21-27
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• 2009

This paper presents the numerical study on the strength safety of the prestressed concrete outer tank for a LNG storage tank, which is manufactured by sets of membrane panels with special corrugations. This study for a finite element analysis assumes that the membrane panel of the inner tank was fractured and the liquefied natural gas stored in the inner membrane tank was leaked to the prestressed concrete outer tank. The stress and displacement of the outer tank have been analyzed for five different loadings, which are originated by a hydrostatic pressure and a weight of a LNG, a temperature difference, a weight of the prestressed concrete and a boil-off gas pressure. The computed FEM results indicate that the PC outer tank with a storage capacity of 200,000$m^3$ has a good strength safety for a leaked LNG from the membrane inner tank, but the increased cryogenic loadings in which are originated by a leaked LNG decreases the strength safety of the PC structure. This may lead to the collapse of the outer storage tank.