• Title/Summary/Keyword: PAS-4

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Ultrastructural and Histochemical Study on the Prostate Gland of a Korean Slug, Incilaria fruhstorferi (한국산 산민달팽이 (Incilaria fruhstorferi) 전립선의 미세구조 및 조직화학적 연구)

  • Chang, Nam-Sub
    • Applied Microscopy
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    • v.27 no.2
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    • pp.155-163
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    • 1997
  • Ultrastructural and histochemical studies were carried out on the prostate gland of a Korean slug (Incilaria fruhstorferi) with light and electron microscopes. The results are as follows. The endothelial tissue of the prostate gland is constituted with tall and narrow ciliated columnar cells, irregularly shaped cells and gland cells in which are included the elongated oval or ellipsoid nucleus those are surrounded by curved membrane. The endothelial tissue of the prostate gland is composed by type-A, B, C and type-D gland cells, and the glanules of type-A, B, and D gland cells are certified to neutral mucopolysaccharide because are related by PAS-alcian blue (pH 2.5) , but the other hand type-C gland cell is only weakly reacted to PAS, but strongly reacted by Million reaction. The glanules of type-A gland cell are small size (about $0.4{\mu}m$) and are seen high electron density, but the glanules of type-B gland cell are large size (about $0.7{\mu}m$) and glanule density is same type-A glanules. Long ellipsoid type-C gland cell contained round nucleus which is well developed beterochromatin in, and that small oval glanules (size, about $0.9{\mu}m$) of moderate high electron density which are formed a group of large glanule together with $4\sim5ea$, but type-D gland cell possessed round small nucleus are seen high electron dense glanules (size, $0.8{\mu}m$).

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Vulnerability Attack for Mutual Password Authentication Scheme with Session Key agreement (세션 키 동의를 제공하는 상호인증 패스워드 인증 스킴에 대한 취약점 공격)

  • Seo Han Na;Choi Youn Sung
    • Convergence Security Journal
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    • v.22 no.4
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    • pp.179-188
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    • 2022
  • Password authentication schemes (PAS) are the most common mechanisms used to ensure secure communication in open networks. Mathematical-based cryptographic authentication schemes such as factorization and discrete logarithms have been proposed and provided strong security features, but they have the disadvantage of high computational and message transmission costs required to construct passwords. Fairuz et al. therefore argued for an improved cryptographic authentication scheme based on two difficult fixed issues related to session key consent using the smart card scheme. However, in this paper, we have made clear through security analysis that Fairuz et al.'s protocol has security holes for Privileged Insider Attack, Lack of Perfect Forward Secrecy, Lack of User Anonymity, DoS Attack, Off-line Password Guessing Attack.

Development of a Transient ihpRNA-induced Gene Silencing System for Functional Analysis in Persimmon (Diospyros kaki Thunb.)

  • Mo, Rongli;Zhang, Na;Yang, Sichao;Zhang, Qinglin;Luo, Zhengrong
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.314-323
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    • 2016
  • A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5' and pHG-PDS3') targeted DkPDS, which also led to significantly reduce expression of DkPDS in 'Mopanshi' persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in 'Mopanshi' and 'Eshi 1' leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in 'Mopanshi', 'Eshi 1' and 'Youhou' leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.

Design for System Architecture of Multiple AVPs with Fail-safe based on Dynamic Network (Fail-safe를 적용한 다수 AVP 차량 및 아키텍처 설계)

  • Woo, Hoon-Je;Kim, Jae-Hwan;Sung, Kyung-Bok;Kim, Jung-Ha
    • Journal of Institute of Control, Robotics and Systems
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    • v.18 no.6
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    • pp.584-593
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    • 2012
  • This paper introduces an AVP (Automated Valet Parking) system which applies an autonomous driving concept into the current PAS (Parking Assistant System). The present commercial PAS technology is limited into vehicle. It means vehicle only senses and controls by and for itself to assist the parking. Therefore, the present PAS is restricted to simple parking events. But AVP includes wider parking events and planning because it uses infra-sensor network as well as vehicle sensor. For the realization of AVP, the commercial steering system of a compact vehicle was modified into steer-by-wire structure and various sensors like LRF (Long Range Finder) and camera were installed in a parking area. And local & global server decides where and when the vehicle can go and park in the testing area after recognized the status of environment and vehicle from those sensors. GPS solution was used to validate the AVP performance. More various parking situations, vehicles and obstacles will be considered in the next research stages based on these results. And we expect this AVP solution with more intelligent vehicles can be applied in a big parking lot like a market, an amusement park, etc.

Pulmonary artery sarcoma manifesting as a main pulmonary artery stenosis diagnosed by 18F-FDG PET/CT (18F-FDG PET/CT를 통해 진단된 주폐동맥 협착 소견의 폐동맥 육종)

  • Lee, Hoonhee;Park, Han-bit;Cho, Yun Kyung;Ahn, Jung-Min;Lee, Sang Min;Lee, Jae Seung;Kim, Dae-Hee
    • Journal of Yeungnam Medical Science
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    • v.34 no.2
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    • pp.279-284
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    • 2017
  • Pulmonary artery sarcoma (PAS) is a rare and fatal disease that often mimics chronic thromboembolic pulmonary hypertension (CTEPH); therefore, diagnosis of PAS is often delayed. Herein, a healthy 74-year-old man was presented with a 4-month history of dyspnea. Chest computed tomography showed wall thickening and stenosis in the main pulmonary artery as well as in both proximal pulmonary arteries. In order to differentiate between unusual CTEPH, vasculitis, and PAS, we performed right heart catheterization and pulmonary angiography. The mean pulmonary arterial pressure was 21 mmHg, and there was severe pulmonary artery stenosis. Thrombi on the pulmonary arterial wall lesions were observed in intravascular ultrasound and optical coherence tomography. Furthermore, the patient had a history of deep vein thrombosis. Therefore, we diagnosed unusual CTEPH. After 6 months of rivaroxaban anticoagulation therapy, a chest X-ray revealed a left lower lobe lung mass, and a positron emission tomography later showed hypermetabolic lesions in the main pulmonary artery wall, in both pulmonary arteries walls, in the lung parenchyma, and in the bones. A biopsy of the right proximal humerus lesion revealed undifferentiated intimal sarcoma. Pulmonary sarcoma is rare, but should be considered when differentially diagnosing main pulmonary artery wall thickening and stenosis. A positron emission tomography may aid in this diagnosis.

Changes in Plasminogen Activity in Uterus Tissue during the Estrous Cycle in the Pigs

  • Kim, Kang-Hyun;Lee, Yong-Seung;Gu, Ha-Na;Yang, Boo-Keun;Cheong, Hee-Tae;Park, Choon-Keun
    • Reproductive and Developmental Biology
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    • v.35 no.4
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    • pp.463-468
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    • 2011
  • This study investigated the changes of plasminogen activators (PAs) activity, expression and localization of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) during the estrous cycle in pigs. Estrous cycle was sorted into three group by pre-ovulation (Pre-Ov), post-ovulation (Post-Ov) and early to mid-luteal stages (Early to mid-L). Analysis for immunohistochemistry was confirmed by location of tPA and uPA. Porcine uterus tissue was cut into $1{\times}1$ cm squares, and were incubated in DMEM/F-12 medium for 1 h at $38^{\circ}C$, 5% $CO_2$ for measurement of PA activity. Western blotting was implemented for measurement of PA quantity. In results, the blood vessels and secretory glands were increased in Post-Ov stage than Pre-Ov and Early to mid-L stages. The tPA and uPA was located mainly within lumen of blood vessels and secretory glands. The PA activity in Post-Ov ($0.99{\pm}0.03$) stage were significantly (p<0.01) higher than Pre-Ov stage ($0.51{\pm}0.03$) and Early to mid-L stage ($0.21{\pm}0.04$). Expression of PAs were significantly (p<0.05) higher in Early to mid-L stage than other stages. These results indicate that PAs activity and expression may change in uterus tissue during the estrous cycle in pigs.

Effect of Cold Stress on Myocardium of Aging Rat (Cold Stress가 노화(老化) 흰쥐 심근(心筋)에 미치는 영향(影響))

  • Kim, Jeong-Ki;Chung, Hyeung-Jae;Lee, Yong-Deok;Park, Won-Hark
    • Applied Microscopy
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    • v.28 no.1
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    • pp.21-38
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    • 1998
  • The present study was performed to determine the effect of cold stress on myocardium of aging rat. Control groups, which aged 6, 12 and 24 months, were compared with age-matched experimental groups that were exposed to moderate cold stress for a hours daily in a week at laboratory cold room $(4{\pm}1^{\circ}C)$. The histological, histochemical and ultrastructural changes of myocardium were observed. The results were summarized as follow: 1. Age-dependent histological change of control groups was observed the formation of contraction band in 24months aged group. The experimental groups submitted to cold stress showed a similar change pattern as seen in control groups. However, the degree of change in the experimental groups was significantly larger than that of control groups. In the 34 months aged group the formation of hypercontraction band was observed. 2. Regarding age-dependent histochemical changes of control groups, we observed the increase activities of PAS and Masson's trichrome. In experimental groups the activities of PAS and Masson's trichrome were also increased with age. Compare with control group, the activities of PAS was increased but the activities of Masson's trichrome was decreased. 3. Age-dependent ultrastructural changes on vacuolization, lysosome were observed. In control groups the structural changes occur at 12 months. The accumulation of lipofuscin, contraction band, hypercontraction band and a component of connective tissue were observed in 24 months. However, the degree of change in the experimental groups was significantly larger than that of control groups. In contract, the myelin body in intercalated discs was observed in 24 months of experimental groups.

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Characterization of Coagulation on Synthetic Polymerization Al(III) Inorganic Coagulants for Water Treatment (상수처리용 합성 무기고분자 Al(III)계 응집제의 응집특성)

  • Han Seung-Woo;Jung Chul-Woo;Kang Lim-Seok
    • Journal of Environmental Science International
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    • v.8 no.6
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    • pp.717-724
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    • 1999
  • This experiment was performed on three parts with prepared coagulants. (1) The characterization of coagulation for PACI coagulants. (2) Comparison of the characterization of coagulation with PAS and PACI coagulants. And (3) Comparison of the characterization of coagulation for the addition of calcium with PACI. Coagulation experiments were conducted with several dosages and pH for each coagulants. For the characterization of coagulation with PACI coagulants, coagulation of Nakdong river waters with three PACls (r=2.0, 2.2, 2.35) showed that the effectiveness of the three coagulants can be considered as r=2.2 > 2.0 > 2.35 which are also the order of higher polymeric aluminum contents. For the comparison of the characterization of coagulation for PAS and PACI coagulants, PAS (r=0.75) coagulants was more effective than other coagulant for the removal of organic matters by sweep floc mechanism with $A;(OH)_{3(S)}$. For comparison of the characterization of coagulation for the addition of calcium with PACI, the presence of divalent cation like $Ca^{2+}$ was supposed to influence the complex formation of organic anions. From the result of test on coagulation at various pH ranges, the PACI was least affected by the coagulation pH, and the addition of calcium to PACI was very effective for the removal of turbidity and organic materials over broader pH range (pH 4-9).

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