• BMB Reports
• /
• 제39권1호
• /
• pp.91-96
• /
• 2006

Previously, we have shown that nitric oxide (NO) directly activates the Maxi-K channels. In the present study, we have investigated whether NO has prolonged effects on the Maxi-K channels reconstituted in lipid bilayer. Application of S-nitroso-N-acetyl-D, L-penicillamine (SNAP), a NO donor, induced an immediate increase of open probability (Po) of Maxi-K channel in a dose-dependent manner. When SNAP was removed from the cytosolic solution, the Po did not simply returned to, but irreversibly decreased to a level lower than that of the control Po. At 0.2 mM, (Z)-[N-(3-Ammoniopropyl)-N-(n-propyl)amino] diazen-1-ium-1,2-diolate (PAPA-NO), another NO donor, produced a similar increase of Po and decrease of Po upon washout. The increasing effects of SNAP on Po were not blocked by either 50 U/ml superoxide dismutase (SOD) or 2 mM N-ethylmaleimide (NEM) pre-treatments. However, NEM appears to be ineffective when applied after SNAP. These results suggest that NO can modulate Maxi-K channel via direct interaction and chemical modification, such as S-nitrosylation in the brain.

• 대한후두음성언어의학회지
• /
• 제11권2호
• /
• pp.167-171
• /
• 2000

Nasal obstruction due to nasal deformities, rhinitis, sinusitis, or nasal polyps etc. induces hyponasality, which can influence the articulation and/or phonation of patients. However few studies were done on the subjects. Therefore, this study was performed to establish the guideline that can be used in diagnosing the hyponasality. We analyzed the nasalance scores of 6 different sentences for 26 normal adults. We measured the nasalance scores before simulated nasal obstruction, after one nostril obstruction, and after both nostrils obstruction, while the subjects are reading roe nasal sentences(so-called, 'Mama' sentence, 'Mimi' sentence, and 'Nana' sentence) and three non-nasal sentences('Papa' sentence, 'Bibi' sentence, and 'Tatda' sentence). The change of nasalance score of six sentences were compared in three conditions, that is, without obstruction, with one nostril obstruction, and with both nostrils obstruction. The nasalance scores of one nostril obstruction and both nostrils obstruction were significantly lower than those of no obstruction. The mean nasalance scores of one nostril obstruction and both nostrils obstruction were lower than those of no obstruction by 10.93% and 48.88% in 'Mama' sentence, 3.72% and 5.76% in 'Papa' sentence, 12.28% nd 38.12% in 'Mimi' sentence, 7.28% and 12.96% in 'Bibi' sentence, 13.32% and 34.62% in 'Nana' sentence, 4.27% and 717% in 'Tatda' sentence, respectively. In both nostrils obstruction, the nasalance score of nasal sentences were decreased more than those of non-nasal sentences significantly. The result suggests that nasal sentences can be used as efficient stimuli in assessing the patients suspected of hyponasality. Eventhough non-nasal sentences also induced significant discrimination, the dynamic range was too small.

• Journal of Animal Science and Technology
• /
• 제53권1호
• /
• pp.29-34
• /
• 2011

본 연구에서는 Landrace, Large White와 Duroc 3품종의 3원 교잡 시스템으로 비육돈을 생산하는 9개 농가를 대상으로 Landrace와 Large White 교배를 통해 생산된 $F_1$ 모돈과 Duroc 웅돈 그리고 비육돈을 이용하여 기 보고한 바 있는 돼지 이력추적 및 브랜드육 식별을 위한 13종의 MS marker의 개체판별능과 혈연관계 추정 효율을 실증하였다. 우선 $F_1$ 모돈과 웅돈 즉 부모 집단을 대상으로 API-CALC version 1.0 프로그램을 이용하여 무작위 교배집단, 반형매 교배집단 그리고 전형매 교배집단으로 가정시 개체 판별능이 각 각 $4.94{\times}10^{-34}$, $8.16{\times}10^{-23}$ 그리고 $2.01{\times}10^{-08}$으로 추정되었으며, 비육돈을 포함한 추정치는 $3.74{\times}10^{-35}$, $5.48{\times}10^{-25}$ 그리고 $2.96{\times}10^{-11}$으로 추정되었다. 또한 Cervus version 2.0을 이용하여 친자감별률을 추정한 결과 100% 인 것으로 추정되었다. 이론적으로 산출된 상기의 수치들을 검증하기 위해 PAPA version 2.0 프로그램을 이용하여 비육돈 전체 452두에 대한 친자감별을 실시한 결과 100% 친부모를 확인 할 수 있었으며, Cervus 프로그램을 이용하여 전체 축군에서 동일한 대립유전자형을 가진 개체의 출현을 조사한 결과 동일개체는 존재하지 않는 것을 확인하였다. 비록 개체 판별능에 대한 실증은 제시한 이론적 판별능에 상응하는 두수를 검증하지는 못하였으나, 친자확인의 경우는 제시한 이론적 효율성 100%는 실증적으로 검증되었다. 따라서 현재 국내에서 행해지는 3 품종 교잡에 의한 비육돈 생산 시스템의 경우 본 연구의 결과로 비추어 볼 때 13 MS marker를 이용하여 체계화된 전산정보와 병행하여 계열화된 생산체계하의 브랜드 단위 또는 지역별 권역으로 구분하는 이력추적이 충분히 가능하다고 사료된다.