• BMB Reports
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• v.39 no.1
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• pp.91-96
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• 2006

Previously, we have shown that nitric oxide (NO) directly activates the Maxi-K channels. In the present study, we have investigated whether NO has prolonged effects on the Maxi-K channels reconstituted in lipid bilayer. Application of S-nitroso-N-acetyl-D, L-penicillamine (SNAP), a NO donor, induced an immediate increase of open probability (Po) of Maxi-K channel in a dose-dependent manner. When SNAP was removed from the cytosolic solution, the Po did not simply returned to, but irreversibly decreased to a level lower than that of the control Po. At 0.2 mM, (Z)-[N-(3-Ammoniopropyl)-N-(n-propyl)amino] diazen-1-ium-1,2-diolate (PAPA-NO), another NO donor, produced a similar increase of Po and decrease of Po upon washout. The increasing effects of SNAP on Po were not blocked by either 50 U/ml superoxide dismutase (SOD) or 2 mM N-ethylmaleimide (NEM) pre-treatments. However, NEM appears to be ineffective when applied after SNAP. These results suggest that NO can modulate Maxi-K channel via direct interaction and chemical modification, such as S-nitrosylation in the brain.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.11 no.2
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• pp.167-171
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• 2000

Nasal obstruction due to nasal deformities, rhinitis, sinusitis, or nasal polyps etc. induces hyponasality, which can influence the articulation and/or phonation of patients. However few studies were done on the subjects. Therefore, this study was performed to establish the guideline that can be used in diagnosing the hyponasality. We analyzed the nasalance scores of 6 different sentences for 26 normal adults. We measured the nasalance scores before simulated nasal obstruction, after one nostril obstruction, and after both nostrils obstruction, while the subjects are reading roe nasal sentences(so-called, 'Mama' sentence, 'Mimi' sentence, and 'Nana' sentence) and three non-nasal sentences('Papa' sentence, 'Bibi' sentence, and 'Tatda' sentence). The change of nasalance score of six sentences were compared in three conditions, that is, without obstruction, with one nostril obstruction, and with both nostrils obstruction. The nasalance scores of one nostril obstruction and both nostrils obstruction were significantly lower than those of no obstruction. The mean nasalance scores of one nostril obstruction and both nostrils obstruction were lower than those of no obstruction by 10.93% and 48.88% in 'Mama' sentence, 3.72% and 5.76% in 'Papa' sentence, 12.28% nd 38.12% in 'Mimi' sentence, 7.28% and 12.96% in 'Bibi' sentence, 13.32% and 34.62% in 'Nana' sentence, 4.27% and 717% in 'Tatda' sentence, respectively. In both nostrils obstruction, the nasalance score of nasal sentences were decreased more than those of non-nasal sentences significantly. The result suggests that nasal sentences can be used as efficient stimuli in assessing the patients suspected of hyponasality. Eventhough non-nasal sentences also induced significant discrimination, the dynamic range was too small.

• Journal of Animal Science and Technology
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• v.53 no.1
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• pp.29-34
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• 2011

Using the materials collected from nine farms in a three-way cross system to produce commercial pigs produced from F1 sows (Landrace $\times$ Large White) $\times$ Duroc, the power of individual discrimination and parentage of the 13 microsatellite (MS) marker set that has been suggested for individual/brand identification (traceability) was empirically tested. Initially, genotypes of the parental population ($F_1$ sows and Duroc), and commercial pigs were determined and the genotype frequency and polymorphic index were estimated using the Cervus 2.0 program. The probability of identity among genotypes of random individuals, that random half sibs and that of full sib individuals, based on the genotypes from 91 $F_1$ sows and Duroc were expected to be $4.94{\times}10^{-34}$, $8.16{\times}10^{-23}$ and $2.01{\times}10^{-08}$, respectively, using the API-CALC version 1.0 program. When commercial pigs were included, the estimates increased to $3.74{\times}10^{-35}$, $5.48{\times}10^{-25}$ and $2.96{\times}10^{-11}$, respectively. For the empirical verification of the estimated powers of individual discrimination and parentage, the parentage test was performed for 452 commercial pigs using PAPA version 2.0, and individuals with the same genotype were investigated using the Cervus version 2.0 program. Parents for all commercial pigs were successfully estimated and no identical individual was identified in the pedigree. Although the individual discriminating power was not fully verified because of the lack of individuals corresponding with the theoretical power, the 100% efficiency of parentage test was clearly confirmed. Therefore, we believe that the 13 MS marker set in conjunction with management record/information for the pig production kept in a farm/brand should be useful in the pork traceability in a brand unit.