• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.141-141
• /
• 2003

소에 있어서 수정란이식이 활성화되고, 수정란이식 기술이 첨단기술도입의 근간이 되면서, 수태율의 향상은 첨단기술의 활성화에 필수적인 요소가 되었다. 또한 수태율 향상을 위해 임신유지를 도와주는 progesterone의 정기적인 투여나 수정란이식 시기에 hCG 등을 투여하여 수태율을 향상시키고자 여러가지 방법이 시도되고 있으나, 수태율 향상에 대한 명확한 기술이 정립되지 않은 상태이다. 따라서 본 연구는 착상시 황체의 Progesterone의 분비를 촉진시키는 것으로 보고되고 있는 비장유래의 macrophage의 체외배양을 통하여 배양액 중 호르몬적 변화를 관찰하고 착상을 유도하는 기전을 밝히고자 시도하였다. 임신 및 비임신 도축암소의 비장을 채취하여 알루미늄박으로 포장하여 얼음에 채운 뒤 실험실로 운반하였고, 비장을 70% alcohol로 외부를 잘 세척한후 표피를 제거하였다. 표피를 제거한 비장조직을 가위로 잘게 세절하고 buffer A 용액으로 조직속의 세포를 분리하였다. 분리된 세포는 1,700 rpm으로 5분간 3회 세정하였으며, 세정된 세포는 유리 petri dish에 넣어 39$^{\circ}C$, 5% $CO_2$, 95% air인 배양기에서 2시간 동안 배양을 실시하였다. (중략)

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.265-265
• /
• 2004

한우번식우 사육농가와 사육규모가 확대와 소 값 상승으로 인하며 인공수정 및 수정란 이식에 대한 수태율 향상에 많은 관심이 집중되고 있다. 이러한 수태율 향상을 위하여 한우 암소에 배란동기화 처리 후 인공수정 시 황체기능 강화제인 등을 투여하여 황체기능을 강화시키고, 투여시기에 따라 개체에 대한 신체충실지수(BCS)와 혈중요소태질소화합물(BUN)을 분석하였으며, 또한 투여시기에 따른 혈중 Progesteroner의 변화를 조사하였다. 황체기능강화제의 투여 방법은 HCG 투여구는 배란동기화 처리 후 6일째와 7일째에 각각 1500 IU와 3000 IU를 투여하였고, 투여 후 14일째에 혈액을 채취하여 혈중 P4 분석하였다. (중략)

• Korean Journal of Animal Reproduction
• /
• v.16 no.4
• /
• pp.353-361
• /
• 1993

This study was taken to examine external changes, behavioral changes, and endocrine changes such as estradiol-17$\beta$, progesterone, T4 and T3 of female Oreochromis niloticus living in 0$\textperthousand$, 10$\textperthousand$, 20$\textperthousand$, and 30$\textperthousand$ salt concentrations, respectively. The results obtained in these experiments were summarized as follows. In seawater obtained in these experiments were summarized as follows. In seawater challenge test, any fish didn't die in each group such as 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, external body color of fish changed from dark-striped to light-grey color. At the same time, thyroxine and triiodothyronine concentrations significantly(P<0.05) increased, and then were at the highest level in 30 salinity. When fish were adapted from 0$\textperthousand$ to 10$\textperthousand$, feed intake of fish started from the fourth day. From 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, estradiol-17$\beta$ levels were increased gradually. When fish was adapted from 0$\textperthousand$ to 10$\textperthousand$, 20$\textperthousand$ and 30$\textperthousand$, the levels of each progesterone didn't show significant change, and especially showed the lowest peak in 20$\textperthousand$. The greatest thyroxine activity(T4) was observed in 30$\textperthousand$. The levels of and triiodothyronine(T3) significantly changed in all salinities, and its level was at the highest peak in 30$\textperthousand$ salinity. Correlation coefficients between serum progesterone and triiodothyronine in 10$\textperthousand$ and 30$\textperthousand$ were ＋0.677 and ＋0.843, respectively. Correlation coefficient of serum thyroxine(T4) and triiodothyronine(T3) individuals in 10$\textperthousand$ was ＋0.768, and ＋0.843, respectively.

• Journal of Pharmacopuncture
• /
• v.3 no.1
• /
• pp.177-191
• /
• 2000

In order to investigate the effect of the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu($BL_{23}$) and Taejo($BL_{11}$) loci on the estrogen-deficiency osteoporosis induced by ovariectomy in rats, serum osteocalcin estradiol progesterone follicular stimulating hormone(FSH) calcium(Ca) and phosphorous(P) levels were monitored. The results were as follows : 1. Serum osteocalcin levels were significantly (p<0.01, p<0.05) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 2. Serum estradiol levels were increased In the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group, but Taejo group did not show significant value. 3. Serum progesterone levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 4. Serum FSH levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 5. Serum Ca levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu group compared with control group, but Taejo group did not show significant value. 6. Serum P levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. According to these results, the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo loci have favorable effect on treatment and prevention of osteoporosis induced by ovariectomy in rats.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.59-59
• /
• 2003

ADAM은 metalloprotease/disintegrin domain을 가진 transmembrane glycoprotein으로서 지금까지 30종류 이상의 ADAM 및 10종류 이상의 ADAM-TS 단백질이 알려져 있다. 이들의 기능은 포유동물의 수정 시 sperm-egg binding과 fusion, myoblast fusion, integrin과의 결합 등에 직접 관여하거나, TNF-alpha 등의 생체신호전달물질이 세포로부터 분비될 때에 이들의 구조를 변화시켜 활성화시키는 효소로서의 작용, 그리고 dendritic cell differentiation 등에 관여하는 것으로 알려져 있다. 본 연구에서는 난소가 제거된 생쥐를 이용하여 자궁조직의 ADAM-8, -9, -10, -12, -15, -17 그리고 -TS1의 gene의 발현이 $17 \beta $-estradiol에 의하여 조절되는 지를 알아보았다. 생후 6 - 8주 된 암컷 생쥐의 난소를 제거하고, 2 주 후에 $17 \beta $-estradiol ($E_2$), progesterone ($P_4$) 혹은 이 둘 혼합액 ($E_2 + P_4$)을 sesame oil에 녹여 근육주사하였다. 2, 6, 12 시간 후 각각 자궁 조직을 얻고 유전자의 발현 양상을 알아보기 위하여 시료로부터 total RNA을 추출하여 역전사 중합효소반응 (RT-PCR)을 실시하였다. Densitometry를 이용, rpL7에 대한 ADAMS의 mRNA 발현 양을 상대적으로 분석하였다. 그 결과 ADAM-8과 -15는 6시간째에서, ADAM-10과 -TS1은 2시간째에서 sesame oil을 주사하거나 $P_4$만을 주사한 군보다 E$_2$를 주사한 군에서 mRNA의 양이 현저하게 증가하였고 ADAM-12는 2시간째에서 ADAM-17은 12시간째에서 sesame oil을 주사하거나 $P_$만을 주사한 군보다 E$_2$를 주사한 군에서 mRNA의 양이 현저하게 증가하였다. 이러한 결과로 미루어 ADAM-8, -10, -15 그리고 TS1은 progesterone에 의하여, ADAM-12와 17은 $17 \beta $-estradiol에 의하여 유전자의 발현이 upregulation 되는 것으로 생각되어진다.

• Journal of Embryo Transfer
• /
• v.23 no.1
• /
• pp.59-64
• /
• 2008

The objective of this study is to investigate the changes in concentrations of blood urea nitrogen (BUN) and sex steroid hormones, such as estrogen, progesterone, and testosterone in Korean cattle (Hanwoo) with reproductive disorders and to examine the relationship between BUN and body condition score (BCS) in Hanwoo. The concentration of BUN was 16.2 mg/dl, 17.8 mg/dl, 15.1 mg/dl, 17.9 mg/dl, and 28.3mg/dl in pregnancy, repeat breeding, follicular cyst, luteal cyst, and ovarian atrophy, respectively. In Hanwoo with BCS $2.0{\sim}2.9$, $3.0{\sim}3.5$ and $3.6{\sim}4.0$, the concentration of BUN was 15.8 mg/dl, 17.0 mg/dl, and 17.6 mg/dl, respectively. Fluoroimmunoassay showed that serum estrogen and progesterone levels were decreased in reproductive disorders Hanwoo, such as ovarian atrophy, endometritis, and weak estrus. The testosterone level was significantly decreased in Hanwoo with reproductive disorders compared to that in pregnant Hanwoo ($0.02{\sim}0.03\;ng/ml$ vs 0.13 ng/ml, p<0.05). The progesterone and estrogen concentrations in follicular fluid obtained from ovary with follicular cyst were significantly higher (p<0.05) than those in normal follicle fluid. These results show that there is no relationship between BUN and BCS in Hanwoo, and the concentration of sex steroid hormone in serum and follicular fluid are changed in reproductive disorders Hanwoo.

• The Korean Journal of Zoology
• /
• v.37 no.2
• /
• pp.174-181
• /
• 1994

3 종의 한국산개구리를 사용하여 여포들이 생성하는 스테로이드와 난자의 성숙능이 번식기 근처에서 어떻게 변하는가를 조사하였다. 개구리의 여포를 생체외 배양하면서 이들이 뇌하수체추출물(frog pituitary homogenate, FPH) 혹은 CAMP의 자극으로 생성하는 progesterone(Pa)과 testosterone(T)의 양을 측정하여 이로부터 P4yT의 비율을 구하였으며 여포난자들이 FPH. P4 혹은 12-0-tetrsdecanoyl phorbol-13-acetate(TPA)에 반응하여 성숙을 일으키는 시기를 조사하였다. 북방산개구리의 여포난자는 11월 부터 호르몬에 반응하여 성숙을 일으켰으며 2월에 PJT의 비율이 급격히 증가하였다 참개구리는 5월에 가서of PJT의 비율이 현저히 증가하고 FPH에 반응하여 성숙을 일으켰다. 그러나 P4에 의한 난자성숙은 3월부터 시작하였다 옴개구리는 7월에 P4/T 비율의 증가가 있었으며 이때부터 또한 TPA에 반응하여 난자의 성숙을 일으켰다 따라서 3종의 개구리에서 모두 각각의 번식기 근처에서 PJT의 비가 급격히 증가하고 또한 성숙능이 생긴다는 것을 알 수있었다.

• The Korean Journal of Zoology
• /
• v.39 no.3
• /
• pp.257-265
• /
• 1996

Previously, we demonstrated that estradiol (E$_2$) was produced by medium sized follicles of Rana nigromaculata and Rana dybowskii in vitro. Present experiments were carried out to determine when the growing follicles have obtained the ability to produce E$_2$. Follicles in different growth stages were isolated and cultured for 6 hours in the presence or absence of frog pituitary homogenates (FPH, 0.1 pituitary/2m1) or various steroid precursors (200 ng/2m1). levels of progesterone (P$_4$), 17 -hydroxyprogesterone (17$\alpha$-OHP), androstenedione (AD), testosterone Cr) or E$_2$ in the medium were measured by RIA. The smallest follicles failed to produce steroids, whereas the smaller follicles produced considerable amounts of steroids (211 pg/follicle), and the medium sized follicles produced a large amounts of steroids (1653 pg/follicle) in response to FPH. Addition of pregnenolone (P5) resulted in a marked increase in P$_4$ but not in other steroids by the smallest follicles whereas the treatment resulted in a marked increase in P$_4$, 17$\alpha$-OHP, AD, T and E$_2$by the smaller and medium follicles. When the amounts of steroids are calculated on the basis of unit surface area (pg/mm$_2$), the ability of the smallest follicles to produce P$_4$ from P5 was similar to those of smaller and medium sized follicles. However, the smallest follicles failed to metabolize P$_4$ to other steroids whereas the smaller and medium follicles did. Taken together, the data suggest that the smallest follicles do not response to FPH in terms of steroid production but they have capacity to convert P5 to P$_4$ and that the smaller follicles have potential to produce E$_2$ although much less efficient than medium sized follicles.

• Journal of Veterinary Clinics
• /
• v.14 no.2
• /
• pp.287-296
• /
• 1997

Serial ultrasonographic examinations were daily performed on 8 bitches (5 Maltese and 3 Yorkshire terrier) from day 15 until parturition to determine the size of gestational structures. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0 ng/ml. When the size of fetal and extra-fetal structures according to gestational age was measured, there was a difference between Maltese and Yorkshire terrier in extra-fetal structures (P<0.001), and no difference in fetal structrures (P<0.001). The correlations between the gestational age and the growth in fetal and extra-fetal structures were examined by regression analysis. The extra-fetal structure which showed high correlation from day 20 to 35 was inner chorionic cavity diameter in two breeds. The fetal structures which showed high correlation from day 36 until parturition was fetal head diameter in two breeds. In conclusions inner chorionic cavity diameter was the most accurate for estimation of gestational age and prediction of whelping day from day 20 to 35, and fetal head diameter from day 36 to parturitions respectively. Ultrasonography is very useful tool to evaluate the gestational age and to predict the whelping day in pet dogs.

• Korean Journal of Veterinary Service
• /
• v.27 no.1
• /
• pp.41-52
• /
• 2004

To establish the differential diagnosis and functional status in ovarian cystic cows, progesterone(P$_4$) and estrogen(E$_2$) level of cystic follicular fluid, ultrasonography for measuring the cystic diameter and thickness of cystic wall, and histological findings were investigated in cystic ovaries from slaughtered Korean native cows. Ovarian follicles were classified as systic if the diameter was greater than 25 mm by ultrasonography. Ovarian cysts < 3 mm of cystic wall thickness, < 10 ng/ml P$_4$ concentration and >10 ng/ml E$_2$ concentration were classified follicular cyst, ovarian cysts 3 mm of cystic wall thickness, 10 ng/ml P$_4$ concentration and <10 ng/ml E$_2$ concentration were classified luteal cyst, and ovarian cysts 3 mm of cystic wall thickness, < 10 ng/ml P$_4$ concentration and <10 ng/ml E$_2$ concentration were classified non-functional ovarian cyst, respectively. Also ovarian cysts were classified 8 types by anatomical and hisctological findings. Ovarian cysts with corpus luteum were 3 of 73 cows and ovarian cysts without corpus luteum were 70 cows. The incidence rates of 8 various types of ovarian cysts were as follows; 2Aa 56.2%, 2Ba 20.5% and 2Ab 15.1%, respectively. The incidence rates of ovarian cysts without corpus luteum were follicular cyst 76.7% and luteal cyst 19.2%. The thickness of cystic wall were lAb 3.9 mm, 2Ab 3.3 mm and 2Bb 3.2 mm, and the cystic fluid P$_4$ concentrations were above 10.0 ng/ml in lAb, 2Ab and 2Bb, respectively. There was significantly correlations between the thickness of cystic wall and cystic fluid P$_4$ concentration in ovarian cysts(p<0.05). The ovarian cyst was classified follicular cysts, luteal cyst and non-functional ovarian cyst by hormone analysis. The luteal cyst was accuratly dignosed by cystic wall thickness. But follicular cysts was misdiagnosed 13 cows of 56 cystic cows. The 13 cystic cows was determined as had non-fuctional ovarian cysts. The cystic fluid P$_4$ concentration was 3.3 ng/ml in follicular ovarian cysts and 30.1 ng/ml luteinized ovarian cysts. There was significantly positive correlations between thickness of cystic wall and serum P$_4$ concentration in follicular(r$^2$ =0.59, p<0.001) and luteal cysts(r$^2$=0.65, p<0.001). These results indicated that ovarian cysts had various stages of degeneration and luteal cyst was accuratly diagnosed measurement of cystic wall thickness by ultrasonography, but follicular cysts was not diagnosed only cystic diameter and cystic wall thickness. In conclusion, it is suggest that ovarian cysts was diagnosed by combination of clinical sign and anatomical cystic features.