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Effects of Cellulase Supplementation on Nutrient Digestibility, Energy Utilization and Methane Emission by Boer Crossbred Goats

  • Wang, Lizhi;Xue, Bai
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.2
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    • pp.204-210
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    • 2016
  • This study examined the effect of supplementing exogenous cellulase on nutrient and energy utilization. Twelve desexed Boer crossbred goats were used in a replicated $3{\times}3$ Latin square design with 23-d periods. Dietary treatments were basal diet (control, no cellulase), basal diet plus 2 g unitary cellulase/kg of total mixed ration dry matter (DM), and basal diet plus 2 g compound cellulase/kg of total mixed ration DM. Three stages of feeding trials were used corresponding to the three treatments, each comprised 23 d, with the first 14 d as the preliminary period and the following 9 d as formal trial period for metabolism trial. Total collection of feces and urine were conducted from the 4th d of the formal trial, and gas exchange measures were determined in indirect respiratory chambers in the last 3 d of the formal trial. Results showed that cellulase addition had no effect (p>0.05) on nutrient digestibility. Dietary supplementation of cellulase did not affect (p>0.05) N intake and retention in goats. Gross energy (GE) intake, fecal energy and urinary energy excretion, heat production were not affected (p>0.05) by the cellulase supplementation. Total methane emission (g/d), $CH_4$ emission as a proportion of live weight or feed intake (DM, organic matter [OM], digestible DM or digestible OM), or $CH_4$ energy output ($CH_4$-E) as a proportion of energy intake (GE, digestible energy, or metabolizable energy), were similar (p>0.05) among treatments. There was a significant (p<0.001) relationship between $CH_4$ and live weight (y = 0.645x+0.2, $R^2$ = 0.54), $CH_4$ and DM intake (y = 16.7x+1.4, $R^2$ = 0.51), $CH_4$ and OM intake (y = 18.8x+1.3, $R^2$ = 0.51) and $CH_4$-E and GE intake. Results from this study revealed that dietary supplementation of cellulase may have no effect on nutrient digestibility, nitrogen retention, energy metabolism, and methane emission in goat.

The Crystal Structure of N-(Diphenylmethylene) aminomethylphosphonate (N-(Diphenylmethylene)aminomethylphosphonate의 결정 구조)

  • 김문집;박호종;김대영
    • Korean Journal of Crystallography
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    • v.13 no.2
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    • pp.86-90
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    • 2002
  • The structure of N-(Diphenylmethylene)aminomethylphosphonate has been determined by X-ray diffraction methods. The crystal system is triclinic, space group P(equation omitted), unit cell constants, a=8.967(2) (equation omitted), b=9.309(2) (equation omitted), c= 10.981(2) (equation omitted), α=101.42(2)°, β=92.22(2)°, γ=92.23(2)°, V=896.8(3) (equation omitted), T=296 K, Z=2, D/sub c/=1.227 Mgm/sup -3/. The intensity data were collected on an Enraf-Nonius CAD-4 Diffractometer with graphite monochromated MoKα radiation (λ=0.7107(equation omitted)). The molecular structure was solved by direct methods and refined by full-matrix least-squares to a final R=7.3% for 979 unique observed F/sub o/>4σ(F/sub o/) refections and 209 parameters.

The Study of Statistical Optimization of MTBE Removal by Photolysis(UV/H2O2) (광분해반응을 통한 MTBE 제거에 대한 통계적 최적화 연구)

  • Chun, Sukyoung;Chang, Soonwoong
    • Journal of the Korean GEO-environmental Society
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    • v.12 no.9
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    • pp.55-61
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    • 2011
  • This study investigate the use of ultraviolet(UV) light with hydrogen peroxide($H_2O_2$) for Methyl Tert Butyl Ether(MTBE) degradation in photolysis reactor. The process in general demands the generation of OH radicals in solution at the presence of UV light. These radicals can then attack the MTBE molecule and it is finally destroyed or converted into a simple harmless compound. The MTBE removal by photolysis were mathematically described as the independent variables such as irradiation intensity, initial concentration of MTBE and $H_2O_2$/MTBE ratio, and these were modeled by the use of response surface methodology(RSM). These experiments were carried out as a Box-Behnken Design(BBD) consisting of 15 experiments. Regression analysis term of Analysis of Variance(ANOVA) shows significantly p-value(p<0.05) and high coefficients for determination values($R^2$=94.60%) that allow satisfactory prediction of second-order regression model. And Canonical analysis yields the stationery point for response, with the estimate ridge of maximum responses and optimal conditions for Y(MTBE removal efficiency, %) are $x_1$=25.75 W of irradiation intensity, $x_2$=7.69 mg/L of MTBE concentration and $x_3$=11.04 of $H_2O_2$/MTBE molecular ratio, respectively. This study clearly shows that RSM is available tool for optimizing the operating conditions to maximize MTBE removal.

Clinical Features and Associated Factors of Macrolide-Unresponsive Mycoplasma pneumonia and Efficacy Comparison Between Doxycycline, Tosufloxacin and Corticostreoid as a Second-Line Treatment (마크로라이드 불응성 마이코플라즈마 폐렴의 임상 양상 및 연관 인자와 2차 치료제로서 doxycycline, tosufloxacin 및 corticosteroid의 효능 비교)

  • Han Byeol Kang;Youngmin Ahn;Byung Wook Eun;Seungman Park
    • Pediatric Infection and Vaccine
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    • v.31 no.1
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    • pp.37-45
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    • 2024
  • Purpose: This study aimed to examine the clinical features and determinants of macrolide-unresponsive Mycoplasma pneumoniae pneumonia (MUMP) and to assess the differences in the time to fever resolution between doxycycline (DXC), tosufloxacin (TFX) and corticosteroid (CST) as second-line treatment. Methods: We retrospectively analyzed the medical records of patients under the age of 18 who were admitted to Nowon Eulji University Hospital between July 2018 and February 2020, diagnosed with mycoplasma pneumonia. Macrolide resistance was confirmed by detecting point mutations in the 23S rRNA gene. MUMP was clinically defined by persistent fever (≥38.0℃) lasting for 72 hours or more after the initiation of macrolide treatment. In cases of MUMP, patients were treated with an addition of CST, or the initial macrolide was replaced either DXC or TFX. Results: Out of 157 cases of mycoplasma pneumonia, 83 cases (52.9%) did not respond to macrolides. Patients with MUMP exhibited significantly higher C-reactive protein (CRP) levels (3.2±3.0 vs. 2.4±2.2 mg/dL, P=0.047), more frequent lobar/segmental infiltrations or pleural effusions (56.6% vs. 27.0%, P<0.001; 6.0% vs. 0.0%, P=0.032), and a higher prevalence of 23S rRNA gene mutations (96.4% vs. 64.6%, P<0.001) when compared to those with macrolide-susceptible M. pneumoniae pneumonia. In terms of second-line treatment, 15 patients (18.1%) responded to CST, 30 (36.1%) to DXC, and 38 (45.8%) to TFX. The time to defervescence (TTD) after initiation second-line treatment was significantly shorter in the CST group compared to the DXC (10.3±12.7 vs. 19.4±17.2 hours, P=0.003) and TFX groups (10.3±12.7 vs. 25.0±20.1 hours, P=0.043), with no significant difference observed between the DXC and TFX groups (19.4±17.2 vs. 25.0±20.1 hours, P=0.262). Conclusions: High CRP levels, the presence of positive 23S rRNA gene mutation, lobar or segmental lung infiltration, and pleural effusion observed in chest X-ray findings were significant factors associated with macrolide unresponsiveness. In this study, CST demonstrated a shorter TTD compared to DXC or TFX. Further, larger-scale prospective studies are needed to determine the optimal second-line treatment for MUMP.

Synthesis and Characterization of Air Stable σ-Bonded ortho-carborane Manganese Metal Complexes $1-[Mn(CO)_5]-2-R-1,2-closo-(σ-C_2B_{10}H_{10}$ and Their Conversion to the Stable ortho-carborane Substituted Fischer-type Carbene Compexes 1-[(CO

  • 김세진;김유혁;고재정;강상욱
    • Bulletin of the Korean Chemical Society
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    • v.16 no.7
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    • pp.634-641
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    • 1995
  • The metal-carbon σ-bond cluster complexes 1-Mn(CO)5-2-R-1,2-C2B10H10 (R=CH3 Ia, C6H5 Ib) have been prepared in good yields from readily available carboranyl lithium complexes, 1-Li+-2-R-1,2-C2B10H10- (R=CH3, C6H5), by direct reaction with (CO)5MnBr. These manganese metal complexes are rapidly converted to the corresponding manganese metal carbene complexes, 1-[(CO)4Mn=C(OCH3)(CH3)]-2-R-1,2-C2B10H10 (R=CH3 IIIa, C6H5 IIIb), via alkylation with methyllithium followed by O-methylation with CF3SO3CH3. The crystal structure of IIIb was determined by X-ray diffraction. Thus, complex IIIb crystallizes in the orthorhombic space group P212121 with cell parameters a=15.5537(5), b=19.0697(5), c=7.4286(3) Å, V=2203.4(1) Å3, and Z=4. Of the reflections measured a total of 3805 unique reflections with F2>3σ(F2) was used during subsequent structure refinement. Refinement converged to R1=0.053 and R2=0.091. Structural studies showed that the manganese atom had a slightly distorted pseudo-octahedral configuration about the metal center with the carbene and ortho-carborane occupying the equatorial plane cis-orientation to each other.

The Knowledge, Attitude and Behavior on Smoking in Elementary School Students (초등학생의 흡연지식.흡연태도와 흡연행동에 관한 연구)

  • Lee, Kwang-Ok;Choi, Hye-Young
    • Research in Community and Public Health Nursing
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    • v.11 no.1
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    • pp.209-221
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    • 2000
  • I began the study to identify the issues related to knowledge. attitude and behavior regarding smoking by sixth graders. which will ultimately lead to the development of a smoking prevention program that will not only help deter school-age children from smoking, but also make them aware of the more desirable behaviors and techniques for healthier life. The results are as follows: 1. Smoking Behavior: Of the focus group. 4.15% are current smokers and 18.23% are ever smokers. 2. Correlation between smoking knowledge and ever smoking: Ever smoking sample($11.10{\pm}3.66$) is less knowledge able than the never-smoking sample($12.17{\pm}3.95$), (t=3.23. p=.001). 3. Correlation between smoking attitude and ever smoking: Ever smoking sample($28.12{\pm}8.51$) was less desirable than the never-smoking sample($l2.17{\pm}3.95$). (t=8.24, p=.000). 4. Correlation between smoking Knowledge and smoking attitude: knowledge about smoking and attitude toward smoking are quantitatively correlated in such way that the more knowledgeable the child is about smoking. the more desirable the attitude toward smoking is(r=.17. p=.000). 5. Correlation between socio-anthropological characteristics and ever smoking: family . atmosphere($x^2$=16.49. p=.001), school life ($x^2$=l1.58, p= .003), grades in school( $x^2$=11.89. p=.003), gender($x^2$=8.97. p=.003). friends' gathering place($x^2$=13.19. p=.02), marital status of parents(p* =.03). and family's financial status($x^2$=6.71. p=.035). In addition, Correlation between somking-environmental characteristics and ever smoking: number of friends who smoke($x^2$=76.01. p=.001). information source for smoking($x^2$=48.03. p=.001), whether or not siblings smoke($x^2$=26.07, p=.001), whether or not female relatives smoke ( $x^2$= 15.65. p= .001), whether or not father smokes ($x^2$= 12.10. p= .007), errands to buy cigarettes for someone($x^2$=9.18. p=.010), and whether or not male relatives smoke ($x^2$=8.82. p=.35) 6. Results of the logistic analysis performed to identify the factors correlated to ever smoking show that: one point decrease in attitude score translates to 25.39 times' increase in ever smoking one person decrease in the number of friends who smoke translates to 0.66 times' decrease in ever smoking: the group where the father has quit smoking has 1.40 times more ever smoking than the group where the father does not smoke at all: and likewise, the group where the father currently smokes has 1.40 times more ever smoking than the group where the father has quit smoking. 7. The overall cause-and-effect relationship between the ever smoking and the related factors: attitude toward smoking caused ever smoking by -.43, smoking by friends, by .12, marital status of arents, by .05, school life. by .04, gender, by -.03, and smoking by father, by -.02. Knowledge about smoking (t=-1.67) did not cause significant effects on ever smoking.

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Relationships between Methionine Supply, Nitrogen Retention and Plasma Insulin-like Growth Factor-I in Growing Sheep Nourished by Total Intragastric Infusions

  • Li, Chong;Zhao, Guangyong
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.10
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    • pp.1393-1398
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    • 2011
  • Four 4-month old Charolais${\times}$Dorset male sheep (initial liveweight $25.0{\pm}1.1\;kg$), fitted with rumen and abomasal fistulas and nourished by total intragastric infusions, were used to study the relationships between methionine (Met) supply, nitrogen (N) retention and plasma insulin-like growth factor-I (IGF-I). Four graded levels of Met, i.e. 0 g/16 g N, 1.76 g/16 g N, 3.52 g/16 g N and 7.04 g/16 g N, were infused into abomasums as experimental treatments. The sheep and treatments were allocated in a $4{\times}3$ incomplete Latin square design (Yudon square design). The experiment lasted 3 periods and each period was 10 days. Quadratic correlations were found between Met level (x, g/16 g N) and N retention (y, g/d): y = $-0.03x^2$+0.41x+2.62, $r^2$ = 0.66, n = 12, p = 0.008, and between methionine level (x, g/16 g N) and plasma IGF-I concentration (y, ng/ml): y = $0.80x^2$-4.53x+190.24, $r^2$ = 0.51, n = 12, p = 0.009. No significant correlation was found between plasma IGF-I (x, ng/ml) and N retention (y, g/d) (p>0.05). It was concluded that Met level had a significant influence on N retention and plasma IGF-I concentration whereas IGF-I might not be an important mediator in the regulation of N metabolism by Met in growing sheep nourished by total intragastric infusions.

Preparation and Structure of Re(≡NC_$6H_5)(PMe_3)_2CI_3$

  • 박병규;김영웅;정건수;박희숙;Lee, Soon W.
    • Bulletin of the Korean Chemical Society
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    • v.16 no.9
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    • pp.835-839
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    • 1995
  • Mer,trans-Re(≡NC6H5)(PPh3)2Cl3, Ⅰ, reacted with trimethylphosphine to give a mixture of two stereoisomers, mer,trans-Re(≡NC6H5)(PMe3)2Cl3,Ⅱ, and fac,cis-Re(≡NC6H5)(PMe3)2Cl3, Ⅲ. These compounds could also be prepared from the reaction of Re(≡NC6H5)(PMe3)(PPh3)Cl3 with trimethylphosphine. In both reactions the mer,trans-isomer is a major product. The products have been characterized by NMR, elemental analysis, and X-ray crystallography. Crystal data for Ⅱ: monoclinic space group P21, a=10.053(1) Å, b=10.844(1) Å, c=10.058(2) Å, β=113.45(2)°, Z=2, R(wR2)=0.0348 (0.0894). Crystal data for Ⅲ: monoclinic space group P21/n, a=7.183(2) Å, b=16.983(4) Å, c=15.543(4) Å, β=90.38(2)°, Z=4, R(wR2)=0.0603 (0.1484).

Molecular and Crystal Structure of' Metalaxyl, $C_{15}H_{21}NO_4$ (Metalaxyl, $C_{15}H_{21}NO_4$의 분자 및 결정구조)

  • Keun Il Park;Young Kie Kim;Sung Il Cho;Man Hyung Yoo
    • Korean Journal of Crystallography
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    • v.13 no.3_4
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    • pp.148-151
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    • 2002
  • The molecular and crystal structure of metalaxyl C/sub15/H/sub21/NO₄, was determined by single crystal x-ray diffraction study. Crystallographic data for, title compound P2₁/c, a=7.849(4) Å, b=13.081(5) Å, c=15.100(3) Å, β=101.8(2)°, V= 1517.6(3) ų, Z=4. The molecular. Structure model was solved by direct method and refined by full-matrix least- squares. The final reliable factor, R, is 0.067 for 1694 independent reflections (F/sub o//sup 2/>4σ(F/sub o//sup 2/)). The molecular structure of title compound shows an intramolecular hydrogen bond: Cl2-Hl2A…O1.

INTERRELATION BETWEEN THE ANGLE FORMED BY THE MIDSAGITTAL PLANE AND THE MANDIBULAR ANGLE AND THE HORIZONTAL INCLINATION OF THE CONDYLAR LONG AXIS (시상정중면에서 하악우각부 사이의 각도와 하악과두장축의 수평경사도간의 상호관계)

  • Oh Wan Soo;Choi Soon Chul
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.22 no.1
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    • pp.109-116
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    • 1992
  • To determine the horizontal inclination of the condylar long axis without taking the submentovertex radiograph, the author studied the interrelation between the angle formed by the midsagittal plane and the mandibular angle and the horizontal inclination of the condylar long axis. In 56 subjects, the author measured the angle formed by the midsagittal plane and both mandibular angle using the modified Twirl Bow and the horizontal inclination of the both condylar long axis from submentovertex radiographs. The result were as follows: The mean value of the angle formed by the midsagittal plane and the mandibular angle was l8.50±1.48° in right side and 19.30±1.55° in left side. The mean value of the horizontal inclination of the condylar long axis was 19.25±7.56° in right side and 20.27±7.050 in left side. The interrelation of the two angles was represented as follows: y = 20.31-0.0094× (r = -0.482, p<0.01) in right side and y = 20.64-0.066×(r =-0.301, p<0.05) in left side (y; the horizontal inclination of the condylar long axis, x; the angle formed by the midsagittal plane and the mandibular angle).

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