• Biotechnology and Bioprocess Engineering:BBE
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• v.3 no.2
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• pp.67-70
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• 1998

In order to understand the influence of ferroxidase center on the protein assembly and solubility of tadpole ferrin, three mutant plasmids, pTH58K, pTH61G, and pTHKG were constructed with the aid of site-directed mutagenesis and mutant proteins were produced in Eshcerichia coli. Mutant ferritin H-subunits produced by the cells carrying plasmids pTH58K and pTHKG were active soluble proteins, whereas the mutant obtained from the plasmid pTH61G was soluble only under osmotic stress in the presence obtained from the plasmid pTH61G was soluble only under osmotic stress in the presence of sorbitol and betaine. Especially, the cells carrying pTH61G together with the plasmid pGroESL harboring the molecular chaperone genes produced soluble ferritin. The mutant ferritin H-subunits were all assembled into ferritin-like holoproteins. These mutant ferritns were capable of forming stable iron cores, which means the mutants are able to accumulate iron with such modified ferroxidase sites. Further functional analysis was also made on the individual amino acid residues of ferroxidase center.

• Journal of Korean Society on Water Environment
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• v.24 no.1
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• pp.69-77
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• 2008

This study was conducted to know the characteristics of agricultural non-point source pollutants runoff by rainfall events at the upper catchment of Goseong reservoir in Gonjy city, Chungnam Province. For this study, the monitoring sites of the research catchment were set nineteen during the research period (between June 2005 and October 2006). Average runoff coefficient were observed 0.51 in 2005, 0.71 in 2006, respectively. The correlation coefficient (r) between the rainfall and peak-flow was investigated 0.787. By rainfall events, the water quality of the sites were shown like this : BOD 0.555~9.60 mg/L, T-N 0.01~13.50 mg/L, T-P 0.002~2.952 mg/L, and SS N.D~820.0 mg/L. The strong rainfall intensity was the most important factor of the soil erosion. The gabs of the arithmetic mean concentrations and the flow weighted mean concentrations were observed as the followings : BOD 0.0~29.2%, T-N 0.1~11.4%, T-P 0.4~95.2%, and SS 1.7~57.0% in 2005, and BOD 1.0~11.9%, T-N 0.7~7.3%, T-P 9.9~36.5%, and SS 6.6~36.5% in 2006, respectively. The BOD pollution load was 2,117 kg (36% of the total BOD loading of survey periods) while, T-N was 3,209.0 kg (27.9% of the total T-N loading of survey periods), T-P was 136.4 kg (37.4% of the total T-P loading of survey periods) and SS was 72,733.8 kg (51.8% of the total SS loading of survey periods) in the year 2005. In case of 2006, BOD load was 1,321.7, T-N was 2,845.8, T-P was 42.9, and SS was 16,275.8 kg, respectively.

• The Korean Journal of Zoology
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• v.35 no.4
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• pp.526-533
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• 1992

포유류 배아의 착상기작을 규명하고자 본 연구에서는 착상시기에 수정된 배아가 착상부위를 어떻게 인식하는 지 그리고 착상부위의 분화유발요인이 무엇인지를 조사하였다. 착상시기(임신 제6일)와 착상후 초기배아 발생기간(임신 제9일)중 혈청내 estradiol (E2)과 Progesterone(P4)의 농도를 측정하였으며, 자궁내막 조직을 착상부위 (antimesome trium)와 비 착상부위(mesometrium)로 분리하여 P4의 수용체 농도를 측정하였다. 혈청내 E2의 농도는 임신 제6일군에서 E2처리군에서 가장 높았으며, 임신 제9일에서는 intact 대조군에서 가장 높고 제9일의 모든 실험군의 농도는 임신 제6일군보다 높았다. 혈청내 P4 농도는 임신 제6일과 9일에서 다같이 대조군에서 가장 높으며 처리군 중에서는 P4처리군에서 높아지고 있다. P4 농도 역시 임신 제9일의 모든 실험군에서 제6일의 실험군보다 높았다. P4수용체 농도는 착상부위가 비 착상부위보다 높으며, 대조군(P < 0.01)과 P처리 군(P < 0.05)에서는 유의한 차이로 비 착상부위보다 높았다. 자궁내막조직 착상을 위한 분화에는 P4의 영향이 크며 P4의 혈청내 농도와 핵의 수용체 농도는 모든 실험군에서 상응하는 관계를 나타내었다. 이 결과는 앞서 일차적으로 발표한 알카리성 phosphatase (ALPase) 활성과도 상응하는 것이다.

• BMB Reports
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• v.28 no.6
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• pp.538-545
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• 1995

When DNA replication of simian virus 40 (SV40) is initiated on the replication origin, the regions containing the initiation sites of DNA primase, which participates in the transient RNA primer synthesis for formation of Okazaki fragments in the lagging strand, were chosen as the target sites of antisense RNA for studies of the inhibition of SV40 DNA replication. Four recombinant transcription vectors, pUC-PrI, pUC-PrII, pGEM-PrBS, and pGEM-PrSN, coding antisense RNA, were constructed. Four antisense RNAs (named as I, II, BS, and SN) having the size of 18, 19,58, and 123 nts, respectively, were made from the transcription vectors by in vitro transcription. And then, antisense RNA in the concentration of 2${\mu}m$ were added to COS cells transfected with pATSV-W which is a recombinant plasmid containing the SV40 origin of replication. The inhibitory extent of DNA replication was measured by DpnI resistance and was confirmed by measurement of transient RNA primer synthesis. The result shows that six combinations of antisense RNA (I, II, BS, SN, I+SN, and BS+SN) lead to the inhibition of SV40 DNA replication by up to 85%.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.342-347
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• 2007

Bacteriophage ${\Phi}FC1$ integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (${\sim}50bp$) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.

• Journal of the Korean Institute of Landscape Architecture
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• v.17 no.3
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• pp.35-47
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• 1990

This study was executed to analyze the vegetational landscape structure and administration planning of Heonin Royal Tomb forest by three kinds of ordination techniques (PCA, RA and DCA) and comparison of the couple photographs between 1920s and 1980s. Seventeen sites in the recreation area and fifteen sites in the protective area were sampled with clumped sampling method in June 1988 and five quadrats were examined in each site. Environmental impact grade 3, 4 and 5 area covered 56.4% of the surveyed area and these area should be restored by the input of human energy. Pinus densifora community of actual vegetation covered 8.4%, Alnus japonica 24.2% and Quercus community 40.9% of the total area. And the afforested vegetation of Pinus koraiensis and Pinus rigida covered 23.1 % The recreation area was divided by P. densiflora, P. densiflora-Quercus aliena, A. japonica-Q.aliena, A. japonica and the protective area by Q. acutissima, Q. aliena, A. japonica-Q. aliena.. DCA ordination showed that successional trends of tree species seem to be from P. densiflora, Sorbus alnifolia, Styrax obassia to Q. variabilis Q. serrata in P. densiflora community and from A. japonica through A. ginnala to Q. aliena in A. japonica community of the upper layer. By the comparison of the couple photographs between 1920s and 1980s, we can recognize the change of historical landscape composed of P. densiflora community and those community is succeeded to Q. aliena.

• The Korean Journal of Physiology
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• v.26 no.1
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• pp.45-54
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• 1992

Effects of pH, $PCO_2$, and adenosine on the vascular contractility were investigated in the pig coronary arteries. The helical strips of isolated coronary arteries were immersed in the HEPES or $HCO_3^-/CO_2$-buffered Tyrode's solution equilibrated with 100% $O_2\;or\;95%\;O_2-5%\;CO_2\;at\;35^{\circ}C$. The contraction was recorded isometrically using a force transducer. The amplitudes of contraction induced by ACh, high $K^+$, and electrical Held stimulation (EFS) were decreased by elevating extracellular pH (pHo) and were increased by lowering pHo. A shift from $0%\;CO_2\;to\;5%\;CO_2$ at constant pHo (pH 7.4) reduced the contractions induced by ACh, high $K^+$, EFS. However the contraction induced by 100mM $K^+$ was less influenced by the change of pHo or $CO_2$. The contraction induced by ACh in $Ca^{2+}$free Tyrode's solution as well as the contraction developed by the addition of extracellular of $Ca^{2+}$ were decreased by lowering pHo and were increased by elevating pHo. High $K^+$ (25mM) induced contraction at pH 6.8 was not returned to the level of the contraction at pH 7.4 by the elevation of extracellular. calcium $[Ca^{2+}]_o$. Adenosine-induced relaxation was more significant with 5% $CO_2$ than 0% $CO_2$ in the high $K^+$-induced contraction and was more significant with low pHo than high pHo in the contraction induced by EFS. From the above results, it is suggested that $H^+$ and $CO_2$ inhibit $Ca^{2+}$ influx as well as $Ca^{2+}$ release from intracellular $Ca^{2+}$ storage sites and enhance the relaxing effect of adenosine in the pig coronary artery.

• Journal of Environmental Health Sciences
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• v.19 no.1
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• pp.51-56
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• 1993

This study was performed to find the ability of bituminous coal fly ash to remove fluoride from water and wastewater at different fluoride concentration, reaction temperature, reaction time and pH. The removal of fluoride is favorable at low concentration, high temperature and acidic pH. With the increase in the initial fluoride concentration, the amount adsorbed increased. Adsorption process was exothermic at pH 2.0. Physisorption process was predominant over pH 2.3, while Chemisorption was under pH 2.3. Fluoride uptake by fly ash was conducted the adherence of fluoride on the active surface sites of adsorbent and its intraparticle diffusion within adsorbent. The values of rate constants of adsorption were 3.028X 10$^{-3}$, 4.715X 10$^{-3}$, 2.88X 10$^{-3}$ (min) and intraparticle diffusion were 1.434X10$^{-3}$, 3.012X10$^{-3}$, 5.635X10$^{-3}$ at each temperatures.

• Journal of Integrative Natural Science
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• v.5 no.1
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• pp.18-21
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• 2012

The resistance of tumour cells against cytotoxic drug is significant limitation in successful chemotherapeutic treatment of cancer. To date, no crystal structure is available for human P-gp. We developed homology model for human P-gp NBD2 by using coordinates of transporter associated protein (TAP1). Docking study was performed for 8-geranyl-chrysin (Flavonoids) inhibitor in the NBD2 model. Ligand-protein interactions were determined which indicates that the 8-geranyl chrysin shares two overlapping sites in the cytosolic domains of P-gp, the ATP site and a hydrophobic steroid-binding site.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.4
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• pp.547-555
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• 2020

Objective: Apoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs. Methods: The pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzyme-linked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor. Results: Results showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs. Conclusion: This study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.