• 동의생리병리학회지
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• 제19권3호
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• pp.671-676
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• 2005

In this study, we investigated the antioxidant effect of extract from Monascus pillosus, on the human wild-type p53 and p21 expressing A549 lung epithelial cell line and MCF-7 mammary adenocarcinoma cell line stimulated by NO. $P21^{waf/cip1}$ was identified as a gene induced in senescent cells. It is a cyclin-dependent kinase inhibitor and has been shown to cause cell cycle arrest and apoptosis. While p53-regulated stimulation of p21 appears to be central for the permanent growth-arrest, the role of p21 in p53-triggered cell death is unclear. Low dose of sodium nitroprusside (SNP) induced the development of senescence associated with increased expression of p53 and p21 in A549 cells. Inhibition of p21 transactivating activity requires high level correlates with the amount of p53 necessary to cause cell death. Association of p21 and p53 results in inhibition of p21-stimulated transcription. This requires a higher p53 level than is necessary for transcriptional activation of endogenous p53-responsive gene but correlates well with the level of p53 necessary to cause cell death. Exposure to W-1 inhibited oxidative stresses-induced senescence-like arrest, resulting in a significant reduction in p53 and p21 steady state levels. These results suggest that p53 and p21 play a central role in the onset of senescence. Thus, it is important to emphasize control of oxidative balance in tumor prevention and aging.

• Parasites, Hosts and Diseases
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• 제35권4호
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• pp.259-264
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• 1997

폐흡충 감염시 일어나는 비만세포 (mastcell)의 반응이 감염경로에 따라 어떠한 영향을 미치는지 알아보고자 비호적숙주인 마우스에 폐흡충의 피낭유충 10개를 피하 또는 경구 감염시킨 후 감염 시기별로 진피 내에 동원되는 비만세포의 숫적 변동 및 탈과립률 (%)을 경시적으로 관찰하였다. 피하로 감염시킨 마우스는 감염 1주 ($38.3/\textrm{mm}^2$)부터 진피 내 비만세포의 수가 PBS주입군 (대조군) (범위: $19.4-25.1/\textrm{mm}^2$)에 비해 유의한 수준 (P<0.05)으로 증가하기 시작하여 전 실험기간 동안 증가하였다. 이때 비만세포는 충체의 낭 (cyst)주위의 피하층에도 집중적으로 침윤되어 있었다. 경구로 감염시킨 마우스 는 감염 2주 ($33.5/\textrm{mm}^2$)부터 진피 내 비만세포의 수가 비감염군 (대조군)(범위: $17.4-22.3/\textrm{mm}^2$)에 비 해 유의 한 수준 (p<0.05)으로 증가하기 시작하여 전 실험 기간인 감염 6주 ($38.4/\textrm{mm}^2$)까지 높게 운지 되었다. 진피 내에 동원된 비만세포의 평균 탈과림률 (%)은 피하 감염 및 경구 감염군 모두 감염 2주부 터 6주까지 60%이상을 보인 반면 PBS주입군 (대조군)및 비감염군 (대조군)에 있어서는 전 실험기간 동안 10%정도의 탈과립률이 관찰되었다. 이상의 결과로 보아 폐흡충을 마우스에 감염시켰을 때 일어 나는 진피 내 비만세포 반응은 감염경로와는 상관없이 충체에서 분비되는 배설-분비 항원의 자극과 깊은 관계가 있음을 알 수 있었다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제19권5호
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• pp.253-260
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• 2019

Background: Sometimes general anesthesia is required for dental surgery in pregnant women. Facial bone fractures or neck abscess should be treated immediately. Dental surgery, however, creates a stressful situation that can cause inflammation. Inflammatory responses are a well-known major cause of preterm labor and preterm birth. Here we demonstrate the effects of remifentanil on the factors related to preterm labor and its mechanism of action on amniotic-derived epithelial cells (WISH cells). Methods: WISH cells were exposed to lipopolysaccharide (LPS) for 24 h and co-treated with various concentrations of remifentanil. MTT assays were performed to measure cell viability. To explain the effects of remifentanil on the factors related to inflammation in WISH cells, activation of nuclear factor kappa B ($NF-{\kappa}B$) and p38 and the expression of interleukin $(IL)-1{\beta}$, tumor necrosis factor $(TNF)-{\alpha}$, cyclooxygenase (COX)2, and prostaglandin E $(PGE)_2$ were quantified using western blotting and RT-PCR, respectively. Results: Remifentanil did not affect WISH cell viability. In western blot analysis, co-treatment with remifentanil resulted in decreased phosphorylation of $NF-{\kappa}B$, and expression of COX2 and $PGE_2$ in LPS-induced inflammation, but the results were statistically significant only at low concentrations. Reduction of $IL-1{\beta}$ and $TNF-{\alpha}$ expression was also observed with RT-PCR. Conclusion: Co-treatment with remifentanil does not affect the viability of WISH cells, but reduces the expression of the factors related to inflammation, which can induce uterine contraction and preterm labor. These findings provide evidence that remifentanil may inhibit uterine contraction and preterm labor in clinical settings.

• 한국입자에어로졸학회지
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• 제19권2호
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• pp.21-30
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• 2023

Solar cells, converting abundant solar energy into electrical energy, are considered crucial for sustainable energy generation. Recent advancements focus on nanoparticle-enhanced solar cells to overcome limitations and improve efficiency. These cells offer two potential efficiency enhancements. Firstly, plasmonic effects through nanoparticles can improve optical performance by enhancing absorption. Secondly, nanoparticles can improve charge transport and reduce recombination losses, enhancing electrical performance. However, factors like nanoparticle size, placement, and solar cell structure influence the overall performance. This study evaluates the performance of silver nanoparticles incorporated in a p-i-n structure of perovskite solar cells, generated via aerosol state by the evaporation and condensation system. The silver nanoparticles deposited between the hole transport layer and transparent electrode form nanoparticle embedded transport layer (NETL). The evaluation of the optoelectronic properties of perovskite devices using NETL demonstrates their potential for improving efficiency. The findings highlight the possibility of nanoparticle incorporation in perovskite solar cells, providing insights for sustainable energy generation.

• Reproductive and Developmental Biology
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• 제30권2호
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• pp.119-124
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• 2006

The present study compared the developmental potential of cloned porcine embryos with mesenchymal stem cells (MSCs), fetal fibroblasts (FFs) and cumulus cells (CCs) by assessing the cleavage and blastocyst rate, total cell number, inner cell mass (ICM) ratio and apoptosis. MSCs were isolated by ficoll gradients from femur of -6 month old female pig, and maintained for primary cultures. FFs from a female fetus at ${\sim}30$ day of gestation were established, and CCs were obtained from cumulus oocyte complexes (COCs) aspirated from $3{\sim}6$ mm follicles in diameter. Donor cells at $3{\sim}4$ passage were employed for nuclear transfer (NT). COCs were matured and fertilized in vitro(IVF) as control. Cleavage rate was significantly (P<0.05) higher in IVF than in NT embryos with MSCs, FFs and CCs ($82.7{\pm}8.9%\;vs\;70.6{\pm}5.4,\;68.7{\pm}5.1\;and\;63.4{\pm}5.6%$, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs ($24.5{\pm}2.8\;and\;20.4{\pm}8.3%$) did not differ, but were significantly (P<0.05) higher than NT derived from FFs and CCs ($10.6{\pm}2.7\;and\;9.8{\pm}2.1%$). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs ($35.4{\pm}5.2\;and\;0.40{\pm}0.09%$, respectively) were significantly (P<0.05) higher than those from FFs and CCs ($24.9{\pm}6.2%\;vs\;0.19{\pm}0.16,\;23.6{\pm}5.5\;and\;0.17{\pm}0.16%$, respectively). Proportions of TUNEL positive cells in NT embryos from FFs and CCs ($6.9{\pm}1.5\;and\;7.4{\pm}1.7%$, respectively) were significantly (P<0.05) higher than in MSCs ($4.8{\pm}1.4%$) and IVF ($2.3{\pm}0.9%$). The results demonstrate that MSCs have a greater potential as donor cells than FFs and CCs in achieving enhanced production of cloned porcine embryos.

• Macromolecular Research
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• 제13권4호
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• pp.293-296
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• 2005

Transferrin ($T_{f}$) has been used as a targeting ligand for delivering liposome/interleukin-12 (IL-12) pDNA complexes to cancer cells mostly due to the greater number of transferrin receptors ($T_{f}R$) found on tumor cells than on normal cells. $T_{f}$ was conjugated to liposomes via the reaction of MPB-PE with thiol groups of $T_{f}$ introduced by a heterobifunctional cross-linking agent, N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP). Four days after C26 inoculation when the tumor volume reached ${\sim}100mm^{3}$, tumor-bearing Balb/c mice were injected intravenously with $T_{f}-liposome/IL-12 pDNA$complexes twice a week for 3 weeks. Significant suppression of tumor growth was achieved in the group treated with the $T_{f}-liposome/IL-12 pDNA$ complexes, with a dose of $10{\mu}g$ of IL-12 pDNA showing the highest suppression effect among the tested doses. Similar results were obtained when the therapy was initiated one day after tumor inoculation, although in this case $30{\mu}g$ IL-12 pDNA/$T_{f}-liposome$ complexes showed a significant suppression of tumor growth between 19 and 23 days after tumor inoculation. This result indicates that the transferrin receptor-targeted liposomal system is an efficient delivery agent of therapeutic genes, such as IL-12, in mice and that its potential clinical use warrants further research investigation.

• 한국환경농학회지
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• 제23권4호
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• pp.234-239
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• 2004

[ $NO_3$ ]-N and T-N removal rates of cattail wetland cells were compared with those of reed wetland cells. The examined cells were a part of a pond-wetland system composed of two ponds in series and six wetland cells in parallel. Each wetland cell was 25m in length and 6m in width. Cattails (Typha angustifolia) were transplanted into three cells and reeds Phragmites australis) into another three ones in June 2000. Water of Sinyang stream flowing into Kohung Estuarine lake located in the southern part of the Korean Peninsula was pumped into the primary pond, its effluent was discharged into the secondary pond Effluent from the secondary pond was funneled into each cell. Two cattail and reed cells were chosen for this research. Water quantity and quality of influnt and effluent were analyzed front May 2001 through October 2001. The volume of influent and effluent of the cells averaged about $20.0\;m^3/day$ and $19.3\;m^3/day$, respectively. Hydraulic retention time was approximately 1.5 days. Influent $NO_3$-N concentration for the four cells averaged 2.39 mg/L. Effluent $NO_3$-N concentration far the cattail and reed cells averaged 1.74 and 1.78 mg/L, respectively. Average $NO_3$-N retention rate for the cattail and reed cells by mass was 30 and 29%, respectively. Influent T-N concentration far the four cells averaged 4.13 mg/L. Effluent T-N concentration for the cattail and reed cells averaged 2.55 and 2.61 mgL respectively. Average T-N retention rate for the cattail and reed cells by mass was 39 and 38%, respectively. $NO_3$-N and T-N concentrations in effluent from the cattail cells were significantly low (p=0.04), compared with those from the reed cells. Cattail wetland cells were more efficient for $NO_3$-N and T-N abatement than reed ones.

• 대한한방부인과학회지
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• 제19권3호
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• pp.1-12
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Leonurus sibiricus on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of death cells treated with indicated concentration of Leonurus sibiricus and investigated cell death rate by MTS assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis and then we observed the differential gene expression by western blot analysis. Results : Leonurus sibiricus significantly inhibited the proliferation of uterine leiomyoma cell in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Leonurus sibiricus induced G1 cell cycle arrest. Leonurus sibiricus enhanced the expression of p27 and p53 with cell cycle arrest. Conclusion : These findings suggest that Leonurus sibiricus is a candidate agent for the treatment of uterine leiomyoma. p27, $p53^{1}$ may play an important role in Leonurus sibiricus-induced cell cycle arrest and cell growth inhibition.

• 미생물학회지
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• 제23권1호
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• pp.13-19
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• 1985

제한효소 Acc I을 정제하고 그 효소적 특성을 연구하였다. 300g(wet weight)의 Acinetobacter calcoaceticus 로부터 얻은 crude extract를 sample로 하여 ammonium sulfate fractionation을 거쳐 Heparin-agarose, DEAE-se-phades, Affi,-gel Blue, phosphoceIIulose, hydroxylapatite의 순서로 chromatography를 수행한 결고 0 .28mg의 AccI 제한효소를 얻었다. 효소의 specific activity는 mg당 $1.1{\times}10^{s}$ unit 이었다. 정제된 Acc [제한효소는 10% SDS-polyacrylamide gel electrophoresis에서 한개의 band로 나타났으며 그 분자량은 45,000~1,000이었다. 이 효소는 $MgCl_2$ 존재하에, pH 8.0에서 11.0사이에서 최대의 활성을 보였다. NaCl은 이 효소의 활성에는 필요하지 않았으나 150mN이상에서는 급격한 효소 활성의 감소가 있었다.

• 한국식품영양학회지
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• 제33권3호
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• pp.309-316
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• 2020

In this study, we investigated the protective effects of Ulva lactuca methanol extracts against ultraviolet B (UVB)-induced DNA damage in HaCaT cells. First, the contents of general and antioxidative nutrient contents of Ulva lactuca were measured. The moisture, carbohydrate, crude protein, crude fat and ash were 14.01%, 44.80%, 23.19%, 3.10% and 14.90%, respectively. Magnesium that acts as DNA repair enzyme cofactor was the most abundant mineral followed by Ca, P and Fe. The total phenolic and anthocyanoside contents of Ulva lactuca were 2.69 mg/g and 0.13 mg/g, respectively. Cells treated with Ulva lactuca methanol extracts for 24 hours post UVB exposure increased cell viability in a concentration-dependent manner compared to the non-treated control. Also, Ulva lactuca methanol extracts decreased the levels of UVB-induced DNA damage such as cyclobutane pyrimidine dimer and DNA damage response (DDR) proteins such as p-p53 and p21. These results suggest that Ulva lactuca methanol extracts comprising physiological active substances such as Mg, polyphenols and anthocyanosides promote DNA repair by regulating genes related with DDR.