• Journal of the Korean Data and Information Science Society
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• 제27권6호
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• pp.1609-1619
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• 2016

본 연구는 한국형 웰니스 생활양식 측정도구의 구성타당도와 신뢰도를 확인하기 위한 도구개발 연구이다. 간호대학생 345명이 99문항의 WEL (wellness evaluation of lifestyle)을 작성하였고, SPSS WIN 22.0와 AMOS 18.0을 이용한 탐색적 요인분석과 확인적 요인분석을 통해 측정도구의 구성타당도를 검증하였다. 최종 한국형 웰니스 생활양식 측정도구는 71문항, 14개 하위척도로 구성되었다. 각 하위척도는 4개의 요인구조를 가지는 것으로 확인되었다: 본질적 자기, 사회적 자기, 신체적 자기, 적응적 자기, 모델의 적합도는 ${\chi}^2=225.12$, p<.001, CMIN/DF=3.17, RMSEA=.08, NFI=.87, IFI=.91, CFI=.91로 나타났다. 도구의 집중타당도를 평가한 결과 기각비 (C.R.)는 2.95~16.84로였고, 표준분산추출 (AVE)도 .61~.69로 수용 가능하였다. 하위척도의 내적 일치도 Cronbach's ${\alpha}$는 .55~.87이었다. 본 연구를 통해 한국형 웰니스 생활양식 측정도구가 한국인의 웰니스를 다차원적으로 측정하기에 타당하고 신뢰성있는 도구임을 확인하였다.

• 한국식품영양과학회지
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• 제37권12호
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• pp.1609-1614
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• 2008

국내 주요 생산지인 전남 무안, 전북 부안, 경북 안동 및 경남 창녕의 3개 지역(유어, 장마, 성산)에서 수거된 양파 6종의 휘발성향기성분을 SPME법으로 추출하여 GC/MSD 로 분석하였다. 총 51종의 화합물이 동정되었는데, 이는 함황화합물류(20종), 알데히드류(4종), 케톤류(5종), 에스테르류(11종), 방향족화합물류(4종), 함질소화합물류(2종) 및 기타화합물류(5종)로 구성되었다. 함황화합물류가 총 휘발성 향기성분 함량의 $62.6{\sim}80.3%$를 차지하였으며, 함량 면에서는 안동(4,828 ng/g), 부안(4,627 ng/g), 유어(4,149 ng/g), 장마(2,979 ng/g), 성산(2,758 ng/g) 및 무안(2,171 ng/g) 순으로 많았다(p<0.05). 항산화효과가 있는 5종의 함황화합물류 (2,4-, 2,5-dimetylthiophene, 2-vinyl-1,3-dithiane, 5-methoxy thiazole, 3,5-diethyl-1,2,4-trithiolane)는 안동, 유어, 장마, 부안, 성산 및 무안 순이었고, 항암효과가 있는 함황화합물류 5종((Z)-, (E)-methyl propenyl disulfide, (Z)-, (E)- propenyl propyl disulfide, di-2-propenyl disulfide)은 부안, 유어, 안동, 장마, 성산 및 무안 순으로 많았다.

• 한국산학기술학회논문지
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• 제15권3호
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• pp.1691-1699
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• 2014

IEEE에서는 V2I, V2V 등의 무선 통신 기능을 제공하여 차량 운행의 안전을 증대 시킬 수 있는 WAVE 규격을 정의하고 있다. WAVE 규격에서는 무선 통신이 갖는 보안 취약성을 극복할 수 있도록 메시지의 암호화 및 인증 기능을 지원하고 있다. 본 논문에서는 WAVE 규격에서 지원하고 있는 보안 알고리즘들을 openssl 라이브러리와 C언어로 구현하였으며, 구현된 알고리즘들은 관련 규격들에서 제시하고 있는 테스트 벡터를 이용하여 정상 동작을 확인하고 성능을 측정하였다. 본 논문에서 구현된 보안 알고리즘들은 플랫폼에 독립적으로 구현되어, WAVE 보안 규격의 구현에 활용될 수 있을 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1605-1609
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• 2001

A total of 252, 50 week-old Isa-brown laying hens were randomly allotted to 7 groups of 3 replicates. Mustard meal (MM) which is a by-product from mustard processing plant, was dried under the sun or in a gas heated pan. It contained on DM basis 30-32% CP, 19-22% EE and 12-13% CF. The meal from either drying method was incorporated into the diets at 0, 10, 20 and 30% which was equivalent to the substitution levels for soybean meal at 0, 31, 63 and 94%, respectively. All birds were individually kept in battery cages where feed and water were freely accessed throughout 84 days experimental period. It was found that egg production, feed intake, body weight gain and egg weight significantly decreased with the increased MM level. The inclusion of 20% MM did not show a significant difference in egg production and quality from the control, but produced 6-8% lower egg production. Feed intake was linearly decreased with the MM levels, except the 10% sun dried MM group. Fat deposition of the birds fed MM diets significantly decreased, while kidney weight increased when compared with the control group. However, the weight of thyroid glands and spleen trended to be heavier in the MM groups, but this was not significantly different among dietary treatments. It was concluded, MM from both drying methods could be incorporated in laying hen diets at the level of 10% without any adverse effect.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2060-2065
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• 2016

Characteristics of E. coli O157:H7-specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E. coli serotypes (broad-host coliphages) were compared. The burst sizes of the two groups ranged from 40 to 176 PFU/infected cell. Distributions of the virulence factors stx1, stx2, ehxA, and saa between the two groups were not differentiated. Broad-host-range coliphages showed lower stability at $70^{\circ}C$, in relation to O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerance by reduction of only 22% and 11% phages, respectively, under pH 3 and 70% ethanol for 1 h exposure. Therefore, these results revealed that the O157 coliphages might be more stable under harsh environments, which might explain their effective infection of the acid-tolerant E. coli O157:H7.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.616-623
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• 2017

Ginsenoside Rg3, a saponin extracted from ginseng, has various pharmacological and biological activities; however, its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPK signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth, and adherence of Brucella abortus in Rg3-treated RAW 264.7 cells were significantly decreased compared with the Rg3-untreated control. Furthermore, an apparent reduction of F-actin content and intensity of F-actin fluorescence in Rg3-treated cells was observed compared with B. abortus-infected cells without treatment by flow cytometry analysis and confocal microscopy, respectively. In addition, treating cells with Rg3 decreased the phosphorylation of MAPK signaling proteins such as ERK 1/2 and p38 compared with untreated cells. Moreover, the colocalization of B. abortus-containing phagosomes with LAMP-1 was markedly increased in Rg3-treated cells. These findings suggest that ginsenoside Rg3 inhibits B. abortus infection in mammalian cells and can be used as an alternative approach in the treatment of brucellosis.

• 한국통신학회논문지
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• 제38C권3호
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• pp.227-232
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• 2013

ITS 기술의 발전으로 인해 교통 분야에 관련한 서비스들이 제시되고 있다. 그리고 IEEE WG802.11과 P1609에서 추진하고 있는 WAVE가 차량 간 통신 표준으로 제정되고 있다. WAVE는 빠른 연결과정을 가지며 이동성 측면에서 우수한 특성을 나타낸다. 또한 국제적으로 차량 간 통신을 이용한 국가 프로젝트 및 서비스가 제안되고 있으며, 특히 미국 도로교통안전국에서 진행 중인 VSC-A 프로젝트에 다수의 차량 제조사가 참여하고 있고 차량 간 통신을 이용한 안전 서비스 및 측위 기술들이 제안되고 있다. 이러한 안전 서비스에 대한 모델 및 성능을 위한 차량 간 통신 성능에 대한 규격을 분석하고 제시해야 할 필요성이 있다. 따라서, 본 논문에서는 차량 간 통신을 이용한 추월보조 안전 서비스에 대한 시나리오 및 모델을 제안한다. 이를 이용하여 WAVE 환경 하에서의 네트워크 시뮬레이션을 수행하여 통신 성능을 평가하였다. 향후 차량 간 통신을 이용한 추월보조 서비스의 실차 적용을 위해 고려해야 할 요소인 채널별 전송거리 및 지연시간에 대한 결과를 결론에서 제시하였다.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.271-276
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• 2017

A highly thermostable ${\beta}-(1-4)-glucanase$ (NA23_08975) gene (fig) from Fervidobacterium islandicum AW-1, a native-feather degrading thermophilic eubacterium, was cloned and expressed in Escherichia coli. The recombinant FiG (rFiG) protein showed strong activity toward ${\beta}-{\small{D}}-glucan$ from barley (367.0 IU/mg), galactomannan (174.0 IU/mg), and 4-nitrophenyl-cellobioside (66.1 IU/mg), but relatively weak activity was observed with hydroxyethyl cellulose (5.3 IU/mg), carboxymethyl cellulose (2.4 IU/mg), and xylan from oat spelt (1.4 IU/mg). rFiG exhibited optimal activity at $90^{\circ}C$ and pH 5.0. In addition, this enzyme was extremely thermostable, showing a half-life of 113 h at $85^{\circ}C$. These results indicate that rFiG could be used for hydrolysis of cellulosic and hemicellulosic biomass substrates for biofuel production.

• Korean Journal of Radiology
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• 제22권10호
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• pp.1609-1618
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• 2021

Objective: Arrhythmogenic mitral valve prolapse (MVP) is an important cause of sudden cardiac death characterized by fibrosis of the papillary muscles or left ventricle (LV) wall, and an association between late gadolinium enhancement (LGE) of the LV papillary muscles and ventricular arrhythmia in MVP has been reported. However, LGE of the papillary muscles may be observed in other causes of mitral regurgitation, and it is not limited to patients with MVP. This study was to evaluate the association of LGE of the LV papillary muscles or ventricular wall on cardiac magnetic resonance imaging (CMR) and ventricular arrhythmia in patients with mitral regurgitation. Materials and Methods: This study included 88 patients (mean age ± standard deviation, 58.3 ± 12.0 years; male, 42%) with mitral regurgitation who underwent CMR. They were allocated to the MVP (n = 43) and non-MVP (n = 45) groups, and their LGE images on CMR, clinical characteristics, echocardiographic findings, and presence of arrhythmia were compared. Results: LV myocardial wall enhancement was more frequent in the MVP group than in the non-MVP group (28% vs. 11%, p = 0.046). Papillary muscle enhancement was observed in 7 (7.9%) patients. Of the 43 patients with MVP, 15 (34.8%) showed LGE in the papillary muscles or LV myocardium, including 12 (27.9%) with LV myocardial wall enhancement and 4 (9.3%) with papillary muscle enhancement. One patient with bilateral diffuse papillary muscle enhancement experienced sudden cardiac arrest due to ventricular fibrillation. Univariable logistic regression analysis showed that high systolic blood pressure (BP; odds ratio [OR], 1.05; 95% confidence interval [CI], 1.01-1.09; p = 0.027) and ventricular arrhythmia (OR, 6.84; 95% CI, 1.29-36.19; p = 0.024) were significantly associated with LGE of the papillary muscles. Conclusion: LGE of the papillary muscles was present not only in patients with MVP, but also in patients with other etiologies of mitral regurgitation, and it was associated with high systolic BP and ventricular arrhythmia. Papillary muscle enhancement on CMR should not be overlooked.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.535-541
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• 2017

Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated $Ant21^T$ and Ant22, were isolated from the Antarctic coastal sea water. Strains $Ant21^T$ and Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16S rRNA gene sequences determined that these strains belong to the genus Deinococcus. Through the analyses of the 16S rRNA gene sequences, strains $Ant21^T$ and Ant22 were found to have 97.7% and 97.8% similarity to Deinococcus marmoris DSM $12784^T$ and 97.0% and 97.2% similarity to Deinococcus saxicola AA-$1444^T$, respectively. The sequence similarity with the type strains of other Deinococcus species was less than 96.9% for both strains. Strains $Ant21^T$ and Ant22 shared relatively high 16S rRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of $84{\pm}0.5%$. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genus Deinococcus. The two strains also showed typical chemotaxonomic features for the genus Deinococcus, in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids ($C_{16:0}$, $C_{16:1}$ ${\omega}6c/{\omega}7c$, $iso-C_{17:0}$, and $iso-C_{15:0}$). They grew at temperatures between $4^{\circ}C$ and $30^{\circ}C$ and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level with Deionococcus marmoris, strains $Ant21^T$ ($=KEMB\;9004-167^T$ $=JCM\;31436^T$) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genus Deinococcus, for which the name Deinococcus rubrus is proposed.