• BMB Reports
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• v.34 no.4
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• pp.322-328
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• 2001

Excision and amplification of pre-determined, large genomic segments (taken directly from the genome of a natural host, which provides an alternative to conventional cloning in foreign vectors and hosts) was explored in human cells. In this approach, we devised a procedure for excising a large segment of human genomic DNA, the iNOS gene, by using the Cre/loxP system of bacteriophage P1 and amplifying the excised circles with the EBNA-1/oriP system of the Epstein-Barr virus. Two loxP sequences, each of which serves as a recognition site for recombinase Cre, were integrated unidirectionally into the 5'-UTR and 3'-UTR regions of the iNOS gene, together with an oriP sequence for conditional replication. The traps-acting genes cre and EBNA-1, which were under the control of a tetracycline responsive $P_{hcmv^*-1}$ promoter, were also inserted into the 5'-UTR and 3'-UTR regions of the iNOS gene, respectively, by homologous recombination. The strain carrying the inserted elements was stably maintained until the excision and amplification functions were triggered by the induction of cre and EBNA-1. Upon induction by doxycycline, Cre excised the iNOS gene that was flanked by two ZoxP sites and circularized it. The circularized iNOS gene was then amplified by the EBNA-1/oriP-system. With this procedure, approximately a 45.8-kb iNOS genomic fragment of human chromosome 17 was excised and successfully amplified in human cells. Our procedure can be used effectively for the sequencing of unclonable genes, the functional analysis of unknown genes, and gene therapy.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.70
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• pp.81-107
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• 2016

Purpose : The purpose of this study is to find that Factors in the practical application of LISMB(Logistics Information System Practice Based) on S.P(Smartphone)' Characters. and take in the whole situation of LISMB on S.P' Characters by this research and it is clear from the findings that LISMB on S.P' Characters situations and distinctiveness. Research design, data, methodology : Research model and hypotheses have been developed based on literature review and working-level interview The collected data were analysed with structural equation modeling, examining the hypothesized relationships and mediating effect. Result : The results of research are as follows, First, LISMB on S.P' Characters has a positive effect on a precariousness of operation management. but That has no effect on a precariousness of general management. Second, Consign management and User non-cooperation has a mediating effect on relationships between LISMB on S.P' Characters and general management. Third, Old version system, User non-cooperation and Unskilled in equipment new system has a partly mediating effect on relationships between LISMB on S.P' Characters and operation management. Conclusion : Smart-phon Prevalence rates keeps increasing. Nevertheless, LISMB on S.P' Characters may no be used in Container transportation market. This problem are being blamed for hampering corporate activities and harbor logistics industry development. so we need new way to solve that problem.

• Journal of Digital Convergence
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• v.12 no.3
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• pp.7-16
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• 2014

This study aims to analyze the key logic of the current C-P-N-D ICT ecological system, to find out the shortcomings of the current system, and then to offer policy suggestions for the establishment of a new creative contents industry ecological system; that is, ICCT (Information, Communication, Contents and Technology) System.

• Proceedings of the Korean Institute of Communication Sciences Conference
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• 2004.07a
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• pp.167-167
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• 2004

• Journal of Digital Contents Society
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• v.8 no.3
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• pp.403-410
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• 2007

The P2P(Peer to Peer) business, which has brought the great wave all over the world, was made possible as a user's environment is getting improved through the introduction of high-tech PC, and the access service of high-speed internet. P2P is the form that anyone using computer could be both a provider and a user by searching or connecting to the personal computer each other out of the concept of server and client. In other words, it is the system made by the technique which could share the data connecting between the PC of the person with the information and the person searching the information. Recently a various system of P2P is under the development which could be applied to the electronic commerce among the companies which is not only the file share among the individuals. In this paper, we propose the more effective electronic business system making use of P2P.

• Journal of Photoscience
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• v.12 no.1
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• pp.41-46
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• 2005

We synthesized chiral choleseric liquid crystalline monomer p-phenylene bis-(p-3-acryloxy-2(S)-methylpropoxy)benzoate (1) and achiral nematic liquid crystalline monomer, p-phenylene p-acryloxy-6-hexyloxy-p-octyloxybenzoate (2) for the construction of cholesteric polymer network with a pitch gradient. In this research we attempted to synthesize cholesteric polymer film which have a wide reflection bandwidth in the region of visible wavelength with the synthesized chiral cholesteric monomer 1 and nematic monomer 2 under various photopoly merization conditions. We succeeded to construct cholesteric polymer film network with a pitch gradient covering more than 200 nm bandwidth in the visible wavelength by diffusion controlled photostructuring method.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.779-780
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• 2006

In comparison with a existing passive Healthcare system, a healthcare system applied OSGi framework is spontaneous and excellently expandable. But, OSGi-based healthcare system has security vulnerability which OSGi has the technical feature and restriction in the service support area. In this paper, in order to overcome these vulnerability, we propose the technique that applies JXTA standardized P2P platform in OSGi-based healthcare system.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.4
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• pp.445-455
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• 2014

Purpose: By investigating the characteristics of the students ophthalmic optics under bachelor's degree course in academic credit bank system, this study aimed to utilize this survey as a basic material for the operation and the improvement of academic credit bank system as the continued education for opticians. Methods: A questionnaire survey for the characteristics of the students was conducted on 98 bachelors who registered in the academic credit bank system and an analysis was made out of the result. Results: As the purpose of selecting the academic credit bank system, acquisition of bachelor's degree took the major portion (51.0%); for information route, school or superior at work took the major portion (33.7%); and for educational institution, alma mater took the major portion (31.6%). Family cooperation (72.4%) to the students in the academic credit bank system was higher than workplace cooperation (46.9%) or social cooperation (39.8%). For the time required for the bachelor's degree course in the academic credit bank system, 2 semesters (55.6%) took the major portion, and 38.9% for entering graduate school and 5.6% for university transfer. The result showed a significant difference in the information route for the academic credit bank system (p<.05), selection criteria for educational institution (p<.05) and workplace cooperation (p<.01) depending on the age. It showed a significant difference in the selection purpose for the academic credit bank system (p<.01), average attendance day (p<.05), workplace cooperation (p<.01) and difficulties (p<.01) depending on working place. It showed a significant difference in the selection criteria for educational institution (p<.01) and family cooperation (p<.05) depending on the academic system of their alma mater. It showed a significant difference in the information route (p<.05), the selection criteria for educational institution (p<.01) and workplace cooperation (p<.05). Conclusions: It is necessary for optometrists to study and pay more attention to continuing education, and the operation and support for the academic credit bank system should be made in consideration of the characteristics of students.

• Journal of the Korea Society of Computer and Information
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• v.13 no.7
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• pp.109-115
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• 2008

In the P2P distributed system, such peers don't always keep on-line status. P2P systems are different from the ordinary systems that such peers are organically operated as the elements of system. In this case, users using P2P system are able to loss the reliability. At this point, many P2P studies are tried to resolve these problems. In this paper, we suggest a P2P search algorithm for multidirectional processing about the resources to be downloading. In existing studies, the loading rate is very high to select new resources supported peer, so we suggest new solution in this study.

• Journal of Korea Multimedia Society
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• v.8 no.5
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• pp.707-741
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• 2005

In the multimedia streaming technology, the unicast method require additional cost by an increase traffic according to increase a number of users. The multicast method can solve this problem, but it don't have connection and control information of clients. Now, the most routers support only unicast. Also it has a problem with an exchange of service in other ISP. This paper proposes traffic agent system which is applied unicast and P2P(Peer to Peer) for distance video teaming to overcome this problem. The proposed system decrease the traffic of stream server by control agent, and cut down expenses of network. This system is tested under the distance video leaning using JMF and application software.