• 생명과학회지
• /
• 제17권1호
• /
• pp.105-109
• /
• 2007

참나무 목초액은 예로부터 농축산업에 이용되어 왔으며 최근에는 항균 및 항산화 효과가 있는 것으로 밝혀지면서 목초액에 대한 과학적인 연구가 활발히 진행되고 있다. 참나무 목초액의 항균 및 항산화 활성과 면역기능에 미치는 영향을 조사하였다. 참나무 목초액의 항균활성은 식품 부패균 및 치주질환 원인균에 대하여 항균활성을 조사하였는데 목초액 $50{\mu}l$ 첨가시 대부분의 식품부패균 및 치주질환 원인균의 생육이 현저히 저해되었다. 참나무 목초액의 항산화력은 DPPH법에서 $20{\mu}l$ 첨가시 95%의 라디칼 소거능을 보였으며 SOD유사활성은 $50{\mu}l$ 첨가시 65%의 항산화력을 나타내었다. 참나무 목초액의 항염증효과는 세균의 LPS를 처리하여 유도된 RAW264.7 세포에서 조사되었는데 LPS를 처리하여 유도된 NO 활성을 현저히 감소(86%)시켰으며 이 농도에서 세포독성 없이 높은 면역 효과를 나타내었다.

• Journal of Periodontal and Implant Science
• /
• 제30권1호
• /
• pp.111-120
• /
• 2000

Purpose We designed this study for the purpose of determining the relationship between periodontal disease activity and PLBW, using the evaluation of probing pocket depth, loss of attachment, gingival index, gingival crevicular fluid amount and subgingival microflora. Methods A total of 100 volunteer mothers(mean age 30.44) at the Department of Obstetrics and Gynecology Seoul National University Hospital were selected for this study.Pregnancy outcomes were categorized into cases and controls in two ways. our definition was based on the following; Group 1 : Any PLBW cases Vs. All NBW controls Group 2 : PLBW cases Vs. NBW controls A periodontal exam was performed on the Ramfjord( #16, 21, 24, 36, 41, 44) teeth and Clinical evaluation consisted of probing pocket depth, loss of attachment, gingival index and gingival crevicular fluid amount. Subgingival plaque samples were collected by three sterile #35 paper points. The total number of anaerobic colonies and aerobic bacteria were enumerated after incubation. Antisera to P. gingivalis, P. intermedia, A. actinomycetemcomitans were produced in white rabbits with live whole cells suspensions. The specific fluorescent bacteria obtained by immunofluorescence and total cell counts obtained by dark-field microscopy were counted on four fields. The percent of each specific microorganism in the total cell count was determined. Results Any PLBW and PLBW cases showed significantly greater probing depth and attachment loss than all NBW and NBW controls. Cases group had significantly increased anaerobic bacterial counts compared with control group and no differences in the other microbes. This study confirmed that periodontal disease is a statistically significant risk factor for PLBW by investigating clinical parameters and subgingival plaque analysis.

• International Journal of Oral Biology
• /
• 제48권2호
• /
• pp.9-18
• /
• 2023

The rapid detection of bacteria in the oral cavity, its species identification, and bacterial count determination are important to diagnose oral diseases caused by pathogenic bacteria. The existing clinical microbial diagnosis methods are time-consuming as they involve observing patients' samples under a microscope or culturing and confirming bacteria using polymerase chain reaction (PCR) kits, making the process complex. Therefore, it is required to analyze the development status of substances and systems that can rapidly detect and analyze pathogenic microorganisms in the oral cavity. With research advancements, a close relationship between oral and systemic diseases has been identified, making it crucial to identify the changes in the oral cavity bacterial composition. Additionally, an early and accurate diagnosis is essential for better prognosis in periodontal disease. However, most periodontal disease-causing pathogens are anaerobic bacteria, which are difficult to identify using conventional bacterial culture methods. Further, the existing PCR method takes a long time to detect and involves complicated stages. Therefore, to address these challenges, the concept of point-of-care (PoC) has emerged, leading to the study and implementation of various chair-side test methods. This study aims to investigate the different PoC diagnostic methods introduced thus far for identifying pathogenic microorganisms in the oral cavity. These are classified into three categories: 1) microbiological tests, 2) microchemical tests, and 3) genetic tests. The microbiological tests are used to determine the presence or absence of representative causative bacteria of periodontal diseases, such as A. actinomycetemcomitans, P. gingivalis, P. intermedia, and T. denticola. However, the quantitative analysis remains impossible, and detecting pathogens other than the specific ones is challenging. The microchemical tests determine the activity of inflammation or disease by measuring the levels of biomarkers present in the oral cavity. Although this diagnostic method is based on increase in the specific biomarkers proportional to inflammation or disease progression in the oral cavity, its commercialization is limited due to low sensitivity and specificity. The genetic tests are based on the concept that differences in disease vulnerability and treatment response are caused by the patient's DNA predisposition. Specifically, the IL-1 gene is used in such tests. PoC diagnostic methods developed to date serve as supplementary diagnostic methods and tools for patient education, in addition to existing diagnostic methods, although they have limitations in diagnosing oral diseases alone. Research on various PoC test methods that can analyze and manage the oral cavity bacterial composition is expected to become more active, aligning with the shift from treatment-oriented to prevention-oriented approaches in healthcare.

• 한국수산과학회지
• /
• 제9권3호
• /
• pp.155-162
• /
• 1976

미이용해조류를 주로 한 33종류 41개시료(홍조류 15종 19개시료, 갈조류 14종 18개시료, 녹조류 3종 3개 시료, 해산현화직물 1종 1개시료)의 조단백질, 조지방, 조섬유, 조회분, 가용성무질소물, 아미노태질소 및 총 아미노산의 조성을 조사하였고, 이 중 조단백질 함량이 높은 지충이, 지누아리 및 조단백질함량이 낮은 거머리말, 알쏭이모자반의 4종류를 시료로 하여 조류단백질의 추출율과 추출액중의 단백질의 침전조건에 대하여 조사 검토하였다. 1. 일반적으로 홍조류에는 조단백질함량이 높고 조지방함량이 낮은 것이 많은데 비하여 갈조류 및 녹조류에는 조단백질함량이 낮고 조지방함량이 높은 것이 많은 경향을 보였다. 2. 조단백질함량과 총아미노산량으로 보아 단백질자원으로서 유용한 조류로는 지충이, 부채꼴아크로소리움, 꿩꼬리, 오카무라서실, 검은서실, 지누아리, 진두발, 불등가사리, 돌가사리, 디크티오타, 고리매 등을 들 수 있고, 반대로 큰디크티오타, 코르다리아, 비틀 대모자반, 알쏭이모자반, 외톨개모자반, 도박, 털도박, 미끌도박, 남작파래, 구명갈파래, 거머리말 등은 그 함량이 극히 낮았다. 5. 분말시료를 물로 추출할 때 8시간 동안에 조체의 총조단백류외 $30\~40\%$ 추출되었으며, 조체단백질함량의 다과에 따른 추출율의 차는 별로 없었다. 또한 조류단백질의 추출속도는 4시간 동안에 총추출단백질의 3이상이 추출되었다. 4. 추출액중의 단백질침전법으로서는 methanol 처리법이 가장 효과적이었고, 다음이 가열처리법, TCA 처리법의 순이였으며, pH 조절법이 가장 못하였다. pH 조절법에 있어서는 pH5 부근으로 조절하는 것이 효과저인 경향을 보였다. 5. 추출액중의 단백질의 침전율은 알쏭이모자반의 것이 가장 좋았고 다음이 지누아리. 거머리말의 순이였으며 지충이의 것이 가장 못하였다.