• 제목/요약/키워드: P. florida

검색결과 207건 처리시간 0.022초

느타리버섯속균과 교잡종에 대한 단백질 밴드에 의한 분류 (Comparisons of Soluble Protein Bands for Pleurotus Species and Interspecific Crosses of Pleurotus Species)

  • 김명조;심재욱;이윤수
    • 한국균학회지
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    • 제25권1호통권80호
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    • pp.20-25
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    • 1997
  • 느타리버섯 균주(Pleurotus isolates)와 이들 균주들을 교잡하여 얻어진 균사에서 추출한 단백질을 전기영동하여 균주간의 유전적 유연관계를 밝힌 결과, P. ostreatus 201은 자가교잡 균주 혹은 타가교잡 균주들과 낮은 유연관계를 나타냈다. P. ostreatus 2042도 역시 동일한 결과를 나타냈다. 그러나, P. ostreatus $2042{\times}P$. ostreatus 202, P. ostreatus $2042{\times}P$. sajor-caju, 그리고 ostreatus $2042{\times}P$ ostreatus 900은 높은 상관관계를 나타냈다. P. ostreatus 202와 P. ostreatus 900은 자가교잡 균주 혹은 타가 교잡된 균주들과 낮은 상관관계를 나타내었다. 그러나 자가 교잡된 P. ostreatus와 P. $ostreatus{\times}P$. florida, 그리고 P. florida와 자가교잡된 P. florida는 각각 서로 높은 상관관계를 나타냈다.

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균사생장(菌絲生長)이 부진(不進)한 사철느타리 및 느타리버섯으로부터 Virus입자(粒子)의 분리(分離) (Virus-like Particles from Abnormal Growing Oyster Mushrooms, Pleurotus florida and P. ostreatus)

  • 고승주;차동열;신관철
    • 한국균학회지
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    • 제20권2호
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    • pp.149-153
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    • 1992
  • 균사생장(菌絲生長)이 부진(不進)한 사철느타리버섯 (P. florida), 느타리버섯 (P. ostreatus) 및 이들 두 종(種)의 교배균주(交配菌株)로부터 30nm 및 23nm 의 구형(求刑) virus입자(粒子)를 단일(單一) 혹은 혼합형태(混合形態)로 분리(分離)하였다. 사철 느타리버섯으로부터 분리(分離)한 virus의 최적(最適) spectrum은 259.5nm 였다.

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Isolation, Regeneration and PEG-Induced Fusion of Protoplasts of Pleurotus pul-monarius and Pleurotus florida

  • Eyini, M.;Rajkumar, K.;Balaji, P.
    • Mycobiology
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    • 제34권2호
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    • pp.73-78
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    • 2006
  • Inter-specific hybridization between Pleurotus pulmonarius and P. florida was attempted through PEG-induced protoplast fusion to select a fusant. The protocol for protoplast release, regeneration and fusion in these two Pleurotus species was standardized using the variables controlling the process. The mixture of mycolytic enzymes, i.e. commercial cellulase, crude chitinase and pectinase, KCl (0.6 M) as osmotic stabilizer, pH 6 of the phosphate buffer and an incubation time of 3 hours resulted in the maximum release of protoplasts from 3-day-old mycelia of P. florida ($5.3{\sim}5.75{\times}10^{7}$ protoplasts/g) and P. pulmonarius ($5.6{\sim}6{\times}10^{7}$ protoplasts/g). The isolated protoplasts of P. florida regenerated mycelium with 3.3% regeneration efficiency while P. pulmonarius showed 4.1% efficiency of regeneration. Polyethyleneglycol (PEG)-induced fusion of protoplasts of these two species resulted in 0.28% fusion frequency. The fusant produced fruiting bodies on paddy straw but required a lower temperature of crop running ($24{\pm}2^{\circ}C$) than its parents which could fruit at $28{\pm}2^{\circ}C$. The stable fusant strain was selected by testing for the selected biochemical markers i.e. Carbendazim tolerance and utilization of the lignin degradation product, vanillin.

볏짚 발효사료 제조시 과산화수소 전처리 효과 (The Effects of Hydrogen Peroxide Pretreatment on Rice Straw Fermentation for Feed)

  • 최윤희;이상복;김명숙;홍재식
    • Applied Biological Chemistry
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    • 제37권5호
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    • pp.326-333
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    • 1994
  • 볏짚의 사료가치를 증진시키기 위하여 과산화수소에 의한 전처리와 섬유소 및 리그닌분해력이 강한 Pleurotus florida 균주로 발효사료를 제조하고 화학성분, 결정도 및 in vitro 소화율을 조사하여 사료적 가치를 검토하였다. P. florida에 의한 볏짚발효시 hemicellulose, cellulose 및 lignin은 각각 22.5%, 11.4%, 28.1% 감소하였고, $H_2O_2$$H_2O_2(pH\;11.5)$ 전처리 후 발효시에는 4% 이하의 저농도에서 감소가 심하였으며 발효기간이 경과할수록 cellulose, lignin 등이 감소하였고 아미노산함량은 무처리 볏짚에 비하여 배양 30일 후에 약 28.9% 증가하였으며 4% $H_2O_2$ 전처리 후 발효시는 35.1% 증가하였다. 볏짚 섬유소의 결정도는 $H_2O_2$ 처리 및 P. florida에 의한 발효처리로 감소하였고 $H_2O_2(pH\;11.5)$ 처리시 증가하였으며 $H_2O_2(pH\;11.5)$ 처리 후 수세시의 잔류볏짚의 결정도는 더욱 증가하였고 P. florida에 의한 볏짚배양 30일 후 in vitro 소화율은 17.1% 증가하였으며, $H_2O_2$ 전처리 후 발효시킨 볏짚은 6% 이하의 저농도에서 소화율 상승효과가 있었다.

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Health Disparities between Black Hispanic and Black Non-Hispanic Cervical Cancer Cases in the USA

  • Khan, Hafiz Mohammad Rafiqullah;Gabbidon, Kemesha;Abdool-Ghany, Faheema;Saxena, Anshul;Gomez, Esneider;Stewart, Tiffanie Shauna-Jeanne
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권22호
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    • pp.9719-9723
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    • 2014
  • Background: Globally, cervical cancer is a major public health concern. Cervical cancer is the second most common cancer among women, resulting in approximately 500,000 cases per year. The purpose of this study is to compare disease characteristics between Black Hispanic (BH) and Black non-Hispanic (BNH) women in the US. Materials and Methods: We used stratified random sampling to select cervical cancer patient records from the SEER database (1973-2009). We used Chi-square and independent samples t-test to examine differences in proportions and means. Results: The sample included 2,000 cervical cancer cases of Black non-Hispanic and 91 Black Hispanic women. There were statistically significant differences between black Hispanic and black non- Hispanics in mean age at diagnosis (p<0.001), mean survival time (p<0.001), marital status (p<0.001), primary site of cancer (p<0.001); lymph node involvement (p<0.001); grading and differentiation (p<0.0001); and tumor behavior (p<0.001). Black women were more likely to develop cervical cancer and to have the highest mortality rates from the disease. Conclusions: Findings from this study show clear racial and ethnic disparities in cervical cancer incidence and prognosis that should be addressed.

Progress in Novel Oxides for Gate Dielectrics and Surface Passivation of GaN/AlGaN Heterostructure Field Effect Transistors

  • Abernathy, C.R.;Gila, B.P.;Onstine, A.H.;Pearton, S.J.;Kim, Ji-Hyun;Luo, B.;Mehandru, R.;Ren, F.;Gillespie, J.K.;Fitch, R.C.;Seweel, J.;Dettmer, R.;Via, G.D.;Crespo, A.;Jenkins, T.J.;Irokawa, Y.
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제3권1호
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    • pp.13-20
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    • 2003
  • Both MgO and $Sc_2O_3$ are shown to provide low interface state densities (in the $10^{11}{\;}eV^{-1}{\;}cm{\;}^{-2}$ range)on n-and p-GaN, making them useful for gate dielectrics for metal-oxide semiconductor(MOS) devices and also as surface passivation layers to mitigate current collapse in GaN/AlGaN high electron mobility transistors(HEMTs).Clear evidence of inversion has been demonstrated in gate-controlled MOS p-GaN diodes using both types of oxide. Charge pumping measurements on diodes undergoing a high temperature implant activation anneal show a total surface state density of $~3{\;}{\times}{\;}10^{12}{\;}cm^{-2}$. On HEMT structures, both oxides provide effective passivation of surface states and these devices show improved output power. The MgO/GaN structures are also found to be quite radiation-resistant, making them attractive for satellite and terrestrial communication systems requiring a high tolerance to high energy(40MeV) protons.

성인형 치주염 환자에 있어 manual probe의 Florida probe의 임상적 비교 (Clinical Comparison Of Manual Probe With Florida Probe In Adult Periodontitis)

  • 유향미;정진형
    • Journal of Periodontal and Implant Science
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    • 제26권1호
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    • pp.244-254
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    • 1996
  • The periodontal probe is a commonly used instrument to assess periodontal conditions. And so, there has been many studies to develop the accuracy and reproducibility of the periodontal probe. The purpose of this study was to compare two different periodontal probes for measurement reliability and time required to use in subjects with moderate periodontitis. It was done after evaluating reproducibility of probing depth by stent guiding for a Manual probe and a Florida probe in subjects with healthy periodontal condition. The results were as follows 1. In experiment to evaluate the reproducibility of probing depth by stent guiding for a Manual probe and Florida probe in subjects with healthy periodontal condition, there was no major significant difference between intraprobe and interprobe relationships. 2. There were reduced probing measuremint error by using the Florida probe for posterior teeth and by using the Manual probe for anterior teeth of subjects with moderate periodontitis. 3. At proximal area, there was higher measurement error by using the Manual probe than the Florida probe. 4. The mean of pocket depth measurement using Manual probe was signifi cantly higher than that using Florida probe(p<0.05). With increasing pocket depth, interprobe difference increased and reproducibility reduced. 5. There was no significant difference in time required to use between Manual probe and Florida probe(p<0.05). 6. There was slight probing measurement difference between Manual probe and Florida probe at different site, but both probes have similar degrees of reproducibility and similar time required to probe.

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Influence of lateralized versus medialized reverse shoulder arthroplasty design on external and internal rotation: a systematic review and meta-analysis

  • Kevin A. Hao;Robert J. Cueto;Christel Gharby;David Freeman;Joseph J. King;Thomas W. Wright;Diana Almader-Douglas;Bradley S. Schoch;Jean-David Werthel
    • Clinics in Shoulder and Elbow
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    • 제27권1호
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    • pp.59-71
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    • 2024
  • Background: Restoration of external (ER) and internal rotation (IR) after Grammont-style reverse shoulder arthroplasty (RSA) is often unreliable. The purpose of this systematic review was to evaluate the influence of RSA medio-lateral offset and subscapularis repair on axial rotation after RSA. Methods: We conducted a systematic review of studies evaluating axial rotation (ER, IR, or both) after RSA with a defined implant design. Medio-lateral implant classification was adopted from Werthel et al. Meta-analysis was conducted using a random-effects model. Results: Thirty-two studies reporting 2,233 RSAs were included (mean patient age, 72.5 years; follow-up, 43 months; 64% female). The subscapularis was repaired in 91% (n=2,032) of shoulders and did not differ based on global implant lateralization (91% for both, P=0.602). On meta-analysis, globally lateralized implants achieved greater postoperative ER (40° [36°-44°] vs. 27° [22°-32°], P<0.001) and postoperative improvement in ER (20° [15°-26°] vs. 10° [5°-15°], P<0.001). Lateralized implants with subscapularis repair or medialized implants without subscapularis repair had significantly greater postoperative ER and postoperative improvement in ER compared to globally medialized implants with subscapularis repair (P<0.001 for both). Mean postoperative IR was reported in 56% (n=18) of studies and achieved the minimum necessary IR in 51% of lateralized (n=325, 5 cohorts) versus 36% (n=177, 5 cohorts) of medialized implants. Conclusions: Lateralized RSA produces superior axial rotation compared to medialized RSA. Lateralized RSA with subscapularis repair and medialized RSA without subscapularis repair provide greater axial rotation compared to medialized RSA with subscapularis repair. Level of evidence: 2A.

Effects of Geography, Weather Variability, and Climate Change on Potato Model Uncertainty

  • Fleisher, D.H.;Condori, B.;Quiroz, R.;Alva, A.;Asseng, S.;Barreda, C.;Bindi, M.;Boote, K.J.;Ferrise, R.;Franke, A.C.;Govindakrishnan, P.M.;Harahagazwe, D.;Hoogenboom, G.;Naresh Kumar, S.;Merante, P.;Nendel, C.;Olesen, J.E.;Parker, P.S.;Raes, D.;Raymundo, R.;Ruane, A.C.;Stockle, C.;Supit, I.;Vanuytrecht, E.;Wolf, J.;Woli, P.
    • 한국농림기상학회:학술대회논문집
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    • 한국농림기상학회 2016년도 추계 학술발표논문집
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    • pp.41-43
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    • 2016
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Neurospora pyr 4 유전자를 이용한 사철 느타리버섯의 형질전환 (Transformation of Pleurotus florida with Neurospora pyr 4 Gene)

  • 변명옥;유영복;유창현;차동열;조무제
    • 한국균학회지
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    • 제17권4호
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    • pp.209-213
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    • 1989
  • 사철 느타리 버섯 Uracil 요구성 균주를 Aspergillus nidulans ans 1과 Neurospora crassa pyr 4 유전자를 지닌 백터를 이용하여 형질전환시켰다. 사철 느타리버섯 ura 요구성 균주의 원형질체를 pyr 4 유전자를 지닌 백터 pDJB3과 혼합 후 PEG와 $CaCl_2$를 처리하였다. 형질전환 균주는 버섯 최소배지에서 안정되게 생육하였고 southern hybridization 결과도 백터 DNA가 사철 느타리 염색체에 삽입되었다. 형질전환 균주는 단핵성이므로 다른 단핵 균사와 균사 융합 후 자실체 형태, 포자형성 등을 조사하였다. 형질전환이 안된 균주는 톱니형 자실체를 형성한데 비해 형질전환된 균주는 톱니형, 깔대기형, 우산형, 갓 미발육형의 자실체를 형성하였다.

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