• Parasites, Hosts and Diseases
• /
• 제57권1호
• /
• pp.9-15
• /
• 2019

Melting temperature shift ($T_m-shift$) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a $T_m-shift$ method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5' end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of $T_m-shift$ was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The $T_m-shift$ method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of $T_m$- values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was $5.22{\times}10^{-6}$ and $5.28{\times}10^{-6}ng/{\mu}l$ samples of AceP and AtuP, respectively. The accuracy of $T_m-shift$ method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the $T_m-shift$ detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권10호
• /
• pp.1438-1442
• /
• 2003

A method for the determination of nitrogen in ruminant feedstuffs, products and excreta (e.g. milk and urine) using a spectrophotometer is developed, where samples processed for P determination are also used to determine N. Samples are digested with sulphuric acid and subsequently with hydrogen peroxide in Kjeldahl tubes. Digested solutions along with phenol and buffered alkaline hypochlorite reagents are incubated in a water bath at $37^{\circ}C$ for 30 min and presented in the spectrophotometer. The spectrophotometer set at 625 nm measures the concentration of N of each sample. Nitrogen in 261 of the samples was also determined by the classical Kjeldahl method in order to develop a relationship between N determined by the Kjeldahl method (Y) and the colorimetric method (X). The mean value of Y was as high as that of X (0.92 vs. 0.96; p>0.05). The colorimetric method predicted Kjeldahl N highly significantly (Y=0.985X-0.024, $R^2=0.993$, p<0.001; or more simply Y=0.974X, $R^2=0.993$, p<0.001). An analysis of regression found no difference (p>0.05; both t-test and F-test) between colorimetric (0.96% N) and adjusted (0.96% N) N. In comparison with the Kjeldahl method, the analytical capacity of N by colorimetric method increases greatly, where 200-300 determinations of N are possible in a working day. In addition, the system provides an opportunity to use not only the same digested solution for both N and P determination of a particular sample, but also uses the same spectrophotometer to assay both N and P. Therefore, the system may be attractive in situations where both elements of a sample are to be determined. In conclusion, the speed of N determination, low cost, efficient use of labour, time and reagents, fewer items of equipment, and the reduction of environmental pollution by reducing effluent and toxic elements are the advantages of this method of N determination.

• 한국산학기술학회논문지
• /
• 제21권1호
• /
• pp.494-503
• /
• 2020

본 연구의 목적은 전통적 강의식 교수학습방법과 액션러닝 교수학습방법을 활용한 수업이 간호대학생들의 간호리더십과 학업적 자기효능감, 셀프리더십에 미치는 효과 차이를 확인하기 위함이다. 연구대상은 간호대학생을 대상으로 간호리더십 교과명 2학점, 15주 학사일정 중 주 2시간 8주간 액션러닝 학습방법을 통해 그에 따른 효과를 보기 위해 간호리더십과 학업적 자기효능감, 셀프리더십을 측정하였다. 일반 강의식 교수학습방법을 적용한 집단을 대조군으로 하였고, 액션러닝 교수학습방법을 적용한 집단을 실험군으로 선정하였다. 자료 분석은 실험군과 대조군의 간호리더십, 학업적 자기효능감, 셀프리더십과 각각의 하위영역의 차이는 독립표본 t-test로 분석하였으며, 사전, 사후 비교는 대응표본 t-test로 분석 하였다. 연구결과는 액션러닝 교수학습방법 적용 후 학업적 자기효능감(t=.828, p<.000), 셀프리더십(t=8.92, p<.000)은 실험군과 대조군에서 유의한 차이가 있는 것으로 나타났으며, 하위영역으로 간호리더십에서는 리더십 발휘(t=5.06, p<.000), 학업적 자기효능감에서는 자신감(t=2.04, p<.043), 자기조절 효능(t=.126, p<.000)에서 유의한 차이를 보였다. 또한 셀프리더십에서는 행동중심적 전략(t=8.74, p<.000), 인지적 전략(t=7.70, p<.000) 모두 유의한 차이가 있는 것으로 확인되었다. 본 연구의 결론은 최근 대두되고 있는 액션러닝 교수학습방법을 교과과정에 적용하여 그 효과를 검증하였다는 점에서 의의가 있으며, 향후, 액션러닝 교수학습방법을 다양한 환경의 교과과정과 학생들의 수준을 고려하여 적용하고, 그에 따른 효과를 확인하는 연구와 다양한 변수들을 추가하여 광범위한 연구가 필요하리라 본다.

• 수산해양교육연구
• /
• 제28권6호
• /
• pp.1865-1874
• /
• 2016

This study was to identify the factors of ethical values in nursing students. The survey was conducted in two nursing colleges (n=180) using self-reported questionnaire. The data were analyzed with descriptive statistics, t-tests, ANOVA, Pearson's correlation coefficient and multiple regression analyses using SPSS/WIN 21 software. Influencing factors of ethical values were empathy(t=3.436, p=.001), teaching method except lecture(t=3.037, p=.003), and curriculum track at high school(t=2.309, p=.022). The model was statistically significant explaining 16.2% of the variance (F=11.185, p<.001). This study suggests that strategies for increasing nursing students' ethical values include reinforcing empathy, utilizing various type of teaching method except lecture in nursing ethics class, and allowing high school students applying to college of nursing regardless of their curriculum track.

• 지역사회간호학회지
• /
• 제31권3호
• /
• pp.337-347
• /
• 2020

Purpose: The purpose of this study was to construct the education applying Havruta's method for nursing students and to examine its effect on the communication competency, critical thinking competency, and self-leadership. Methods: A non-equivalent control group pretest-posttest design was used. The participants were 138 nursing students at two universities. The experimental group (n=70) received the education applying Havruta's method for six weeks. The study was carried out from September 23 to November 8, 2019. Data were analyzed using IBM SPSS/WIN 22.0 for the 𝑥² test, Fisher's exact test, and independent t-test. Results: The experimental group had a significant increase in communication competency (t=2.44, p=.016). However, there was no significant difference in critical thinking competency (t=1.85, p=.067), and self-leadership (t=1.78, p=.077) between the two groups. Conclusion: The education applying Havruta's method is an effective nursing education for communication competency. The result of this study could be a guide for nursing professors at the nursing education field when developing education programs that apply Habruta's method. Based on these results, future studies should be conducted to apply Habruta's teaching method to various nursing majors.

• 대한환경공학회지
• /
• 제28권11호
• /
• pp.1207-1212
• /
• 2006

본 연구는 담수 퇴적물 중 유기물의 척도가 되는 화학적산소요구량, 염양염류인 총질소와 총인 및 금속류인 철과 망간에 대해 국내 외 분석법을 적용하여 분석을 실시함으로서 각 분석법간의 상관성을 파악하고 그 적용성을 알아보고자 하였다. 화학적산소요구량은 국내 해양환경공정시험방법과 일본의 위생시험법을 적용하였는데 두 방법 모두 측정범위가 넓고, 생물학적산소요구량이나 유기물질과 상관관계가 좋은 과망간산칼륨법을 이용하고 있기 때문에 분석법간 편차가 크지 않음을 확인하였다. 총질소와 총인의 경우 국내 식품공전에 의한 시험법과 일본의 위생시험법을 적용하여 분석을 실시하였다. 총질소는 두 방법 모두 중화 적정법이지만 시료 분해 및 증류 과정의 차이에 의해 일본의 위생시험법에 의한 분석 값이 약간 낮은 경향을 보였다. 총인의 경우 두 방법 모두 질산과 과염소산에 의한 분해 후 값을 측정하는 방법으로 비슷한 결과를 보였다. 철과 망간과 같은 금속류의 실험은 크게 용출방법과 산분해법을 실시하였다. 용출법에서 얻은 결과값은 산분해한 값에 비하여 적은 값을 나타내었는데 그 이유는 용출조건보다 산분해 조건에서 금속류가 더 잘 녹아나오기 때문이다.

• 지역사회간호학회지
• /
• 제18권2호
• /
• pp.310-319
• /
• 2007

Purpose: This study intends to investigate gender role identity and egalitarianism consciousness among adolescents and to determine the relationship between the two factors. Method: Data were collected from 376 middle and high school students in U City. Tools used were the Korean Sexual Role Identity (Kim, J. H., 2005) and the Gender Egalitarianism Consciousness among Adolescents (Kim, 2002). Data were analyzed using SPSS 12.0 for t-test, one-way ANOVA and Pearson correlation coefficient. Results: There was no significant difference between boys and girls in sender role identity. Boys' gender role identity was different according to school grade (t=-3.19, p= .00), frequency of mother's hugging (t=-2.28, p= .02) and girls' according to religion (t=-2.24, p= .03) mother's job (F=3.59, p= .02), father's education level (F=3.20, p= .04), mother's education level (F=3.24, p= .04), family harmony (F=7.28, p= .00), and frequency of mother's hugging (t=-2.41. p= .02). There was significant difference in egalitarianism consciousness according to sender (t=-12.70. p= .00) Boys' egalitarianism consciousness was different according to school type (t= .02, p= .00) and father's education level (F=4.97, p= .02), and girls' according to school grade (t=-4.21 p= .00), school type (t=-3.12, p= .00), father's level of education (F=3.78. p= .02), decision maker of important domestic matter (F=4.95, p= .00) and frequency of mother's hugging (t=2.53, p= .01). In Girls, sexual role identity and sender egalitarianism consciousness were significantly correlated with each other (r= .01, p= .04). Conclusion: Through the study, it was found that boys' concern for sexual equality of was remarkably lower than girls. Therefore, it is necessary to offer curriculums for boys and to start social education for parents.

• 대한화학회지
• /
• 제39권5호
• /
• pp.384-392
• /
• 1995

$BiCl_3$의 승화압과 엔탈피를 370.6에서 488.8 K까지 Knudsen 유출법을 사용하여 측정하였고, 또한 438.2에서 495.7 K까지 Torsion 유출법에 의해 추가 실험을 하였다. 평형 승화압도 이 안정상태 데이터로부터 구하였다. ${\Delta}_{sub}H^0_{298.15}$와 ${\Delta}_{sub}S^0_{298.15}$의 상관관계를 이용하여, 추천할 수 있는 p(T) 식을 $BiCl_3(s)$에 의해 아래와 같이 구하였다. $BiCl_3(s); \; log(p/Pa)=－C/(T/K)\;－2.838log(T/K)－8.097{\times}10^{-2}(K/T)^2＋22.588$, 여기서 p는 파스칼, T는 절대온도, ${\Delta}_{sub}H^0_{298.15}$의 단위는 kJ $mol^{-1}$이고, $C=({\Delta}_{sub}H^0_{298.15}＋5.9988)/1.9146{\times}10^{-2}$이다. 응축상수와 그것의 온도 의존성도 위의 두 방법에 의한 실험으로부터 유도하였다.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2009년도 제38회 동계학술대회 초록집
• /
• pp.472-472
• /
• 2010

The tomography has played a key role in tokamak plasma diagnostics for image reconstruction. The Phillips-Tikhonov (P-T) regularization method was attempted in this work to reconstruct cross-sectional phantom images of the plasma by minimizing the gradient between adjacent pixel data. Recent studies about the comparison of the several tomographic reconstruction methods showed that the P-T method produced more accurate results. We have studied existing Laplacian matrix used in Phillips-Tikhonov regularization method and developed modified Laplacian matrix (Modified L). The comparison of the reconstruction result by the modified L and existing L showed that modified L produced more accurate result. The difference was significantly pronounced when a portion of plasma was reconstructed. These results can be utilized in the Edge Plasma diagnostics; especially in divertor diagnostics on tokamak a large impact is expected. In addition, accurate reconstruction results from received data in only one direction were confirmed through phantom test by using P-T method with modified L. These results can be applied to the tangentially viewing pin-hole camera diagnostics on tokamak.

• 한국세라믹학회지
• /
• 제31권6호
• /
• pp.685-693
• /
• 1994

Sublimation vapour pressures and sublimation enthalpy of anhydrous bismuth trifluoride have been measured by the continuous gravimetric Cnudsen-effusion method from 639-7 to 782.8 K. Additional effusion measurements have also been made from 750.6 to 863.1 K by the torsion-effusion method. Based on a correlation of subHo298.15 and subSo298.15, a recommended P(T) equation has been obtained for BiF3(s); logP= -C/T+5.2375logT-3.205$\times$10-3+4.661$\times$104/T2+1.348 where P is in Pa, T in Kelvin, subHo298.15 in kJ mol-1 and C=( subHo298.15-13.5149)/1.9146$\times$10-2. Condensation coefficients and their temperature dependence have been derived from the effusion measurements.