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Studies on the Removal of Phytate from Korean Rapeseed(Brassica napus, L) Proteins -Ⅰ. Effects of pH and Salts on Protein and Phytate Solubility of Defatted Rapeseed Flour- (한국산 평지 종실 단백질의 Phytate 제거에 관한 연구 -제1보. 평지 종실 단백질과 Phytate 의 용해도에 대한 pH와 염류의 영향-)

  • Huh, Chai-Ok;Yang, Cha-Bum
    • Applied Biological Chemistry
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    • v.29 no.2
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    • pp.212-218
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    • 1986
  • Proteins in Korean rapeseeds, as in many other plantseeds, are usually bound to phytate molecules. These phytate-bound proteins are of little value as foodstuffs because of their poor solubility in digestive systems. Therefore it is necessary to remove phytates from proteins in order to convert these proteins io a useful foodstuff. In the work, an efficient procedure for removal of phytates from defatted Korean rapeseed was found. The influence of pH on the solubility of protein and phytate of rapeseed flour showed that the former was the lowest at pH 5.0 and began to increase as pH further raised. Meanwhile, the latter was the highest at pH 6.0, however, it was decreased abruptly at alkaline pH, especially to content of 1.3% at pH 11.5. The solubility cf protein was relatively high in NaCl aqueous solution at $pH\;6.0{\sim}8.0$, and did not male any noticeable difference depending on NaCl concentration. On the other hand, the solubility of phytate was high at pH of below 6.0 showing an abrupt decrease at pH of above 6.0. The solubility of protein in $CaCl_2$ aqueous solution was highest at $pH\;6.0{\sim}8.0$, however, there was no significant change at the whole range of tested pH of the solution. A maximum solubility of phytate was shown at $pH\;3.0{\sim}4.0$. And it was decreased abruptly at a higher pH of the above range and also decreased at a lower pH with higher $CaCl_2$ concentration. The solubility of phytate in $Na_2SO_3$ aqueous solution was highest at $pH\;5.0{\sim}8.0$. As the concentration goes up the maximum value of solubility was found to move to higher pHs. Depending on the concentration of $Na_2SO_3$, the decreasing pattern was changed in an alkaline solution. The solubility of phytate in the solution containing low concentration of $Ca^{2+}$ ion was low in all treatments at pH of above 7.0 and showed the maximum value at low pH as $Ca^{2+}$ ion concentration increases. The solubility of protein at pH 11.5 showed the highest value in $1mM\;Ca^{2+}$ ion solution.

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Quality Characteristics in Mash of Takju Prepared by Using Different Nuruk during Fermentation (누룩 종류를 달리하여 담금한 탁주 발효과정중 술덧의 품질특성)

  • Han, Eun-Hey;Lee, Taik-Soo;Noh, Bong-Soo;Lee, Dong-Sun
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.555-562
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    • 1997
  • The characteristics of mash qualities of takju prepared by using different nuruk (Korean-style bran koji) such as Mucor racemosus nuruk, Rhizopus japonicus nuruk, Aspergillus oryzae nuruk, Aspergillus kawachii nuruk and traditional nuruk were investigated during fermentation. At the begining of fermentation, ethanol content was in the range of $2.0{\sim}3.0%$. However, it increased to $8.2{\sim}12.6%$ after 16 days of fermentation. Takju made from Rhizopus japonicus nuruk showed higher ethanol content than treated otherwise. pH of takju made from Rhizopus japonicus nuruk showed higher value the others. Total acids were $0.15{\sim}0.20%$ at the begining of fermentation, and it increased to $0.086{\sim}1.57%$ after 16 days of fermentation. Total sugar were $16.64{\sim}17.62%$ at the begining of fermentation, but decreased to below 7.00% after 16 days of fermentation. Rhizopus japonicus nuruk showed the lowest level of total sugar content. Except ethanol, iso-amyl alcohol and iso-butyl alcohol were major part of minor alcohol in the mash of takju. Higher concentration of iso-amyl alcohol, iso-butyl alcohol and n-propyl alcohol were found in the mash of Rhizopus japonicus nuruk whereas the level of phenylethyl alcohol was high in the mash of traditional nuruk. Fusel oil was $0.002{\sim}0.411\;mg/mL$ during fermentation.

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Isolation of Alkaline Amylase-Producing Bacillus sp. and Some Properties of Its Crude Enzyme (알칼리성 아밀라아제를 생산하는 Bacillus속 미생물의 분리와 그 조효소의 특성)

  • Shin, Yong-Chul;Kim, Tae-Un;Lee, Sang-Yeol;Byun, Si-Myung
    • Korean Journal of Food Science and Technology
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    • v.23 no.3
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    • pp.349-354
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    • 1991
  • An alkaline amylase-producing Bacillus sp. GM8901 was isolated and some properties of crude enzyme extract were examined. The microbiological and biochemical characteristics of GM8901 were very similar to those of B. licheniformis. The optimal temperature and pH for the cell growth and amylase production were $50^{\circ}C$ and pH 10.5. The crude amylase extract showed that the optimal temperature and pH were $50{\sim}60^{\circ}C\;and\;pH\;10{\sim}12$, respectively, and that the activity of amylase was stable up to $50^{\circ}C$ and in the range of $pH\;3{\sim}12$.

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INJECTIVE AND PROJECTIVE PROPERTIES OF REPRESENTATIONS OF QUIVERS WITH n EDGES

  • Park, Sangwon
    • Korean Journal of Mathematics
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    • v.16 no.3
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    • pp.323-334
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    • 2008
  • We define injective and projective representations of quivers with two vertices with n arrows. In the representation of quivers we denote n edges between two vertices as ${\Rightarrow}$ and n maps as $f_1{\sim}f_n$, and $E{\oplus}E{\oplus}{\cdots}{\oplus}E$ (n times) as ${\oplus}_nE$. We show that if E is an injective left R-module, then $${\oplus}_nE{\Longrightarrow[50]^{p_1{\sim}p_n}}E$$ is an injective representation of $Q={\bullet}{\Rightarrow}{\bullet}$ where $p_i(a_1,a_2,{\cdots},a_n)=a_i,\;i{\in}\{1,2,{\cdots},n\}$. Dually we show that if $M_1{\Longrightarrow[50]^{f_1{\sim}f_n}}M_2$ is an injective representation of a quiver $Q={\bullet}{\Rightarrow}{\bullet}$ then $M_1$ and $M_2$ are injective left R-modules. We also show that if P is a projective left R-module, then $$P\Longrightarrow[50]^{i_1{\sim}i_n}{\oplus}_nP$$ is a projective representation of $Q={\bullet}{\Rightarrow}{\bullet}$ where $i_k$ is the kth injection. And if $M_1\Longrightarrow[50]^{f_1{\sim}f_n}M_2$ is an projective representation of a quiver $Q={\bullet}{\Rightarrow}{\bullet}$ then $M_1$ and $M_2$ are projective left R-modules.

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Study on characterization of OTFT for patterned active layer P3HT using conventional photolithography (포토리소그래피를 이용한 P3HT 활성층의 패터닝에 의한 OTFT 특성 연구)

  • Park, Kyeong-Dong;Han, Kyo-Yong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.11a
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    • pp.9-10
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    • 2006
  • The patterning for the active layer of organic semiconductors is important to attain completely organic-based OTFTs(Organic Thin Film Transistors). We studied on possibility of the application of the conventional photolithography technique to pattern the organic active layer poly(3-hexylthiophene)(P3HT). Patterned P3HT-based OTFTs with Bottom Contact(BC) configuration were fabricated using the conventional photolithography. We achieved field-effect mobilities in the saturation regime ${\sim}1.2{\times}10^{-3}cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}10^5$ in the subtractive method, ${\sim}8{\times}10^{-4}cm^2/V{\cdot}s$, $I_{on/off}$ ratios ${\sim}10^3$ in the additive one.

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Growth and Flowering Characteristics of Polygonatum spp. (둥굴레 속(屬) 식물(植物)의 생육(生育) 및 개화(開花) 특성(特性))

  • Jang, Key-Hyun;Park, Jung-Min;Kang, Jin-Ho;Lee, Seong-Tae
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.2
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    • pp.142-148
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    • 1998
  • The Polygonatum spp. used in this study was collected from 1994 to 1997, and planted by species in Hamyang medicinal plant experimental station field. $30{\sim}40 $ species of Polygonatum spp. have been known to be distributed in the northern hemisphere, and $14{\sim}18$ species in Korea. In this study, collected seven species of Polygonatum spp. were investigated. Plant height was highest in Polygonatum stenophyllum followed by P. falcatum, P. humile, P. lasianthum var. coreanum.. Leaf length was longest in P. falcatum and shortest in P. odoratum and leaf width was the most wide in P. odoratum var. pluriflorum. P. stenophyllum had 111 flowers per stem. The other species had $14{\sim}22$ flowers per stem. The ratio of flower setting in each species was high at two but was 95.2% at above four in P. stenophyllum. The number of fruits was 14.8, 15.4 and 17.7 in P. odoratum, P. falcatum and P. humile, respectively. Other species did not produce fruit. Fruit setting percentage was high in P. odoratum as 81.5%. The number of branched rhizome doubled every year.

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Environmental Characteristics of Natural Habitat of Protothaca jedoensis in Gamak Bay, Korea (가막만 살조개, Protothaca jedoensis 서식지 환경특성)

  • Yoon, Ho-Seop;Choir, Sang-Duk
    • Journal of Aquaculture
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    • v.19 no.1
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    • pp.7-13
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    • 2006
  • In this study, environmental parameters of natural habitat were measured to find out suitable place for release and improve the productivity of Protothaca jedoensis in Gamak Bay. Water temperature, salinity of the habitat ranged from 5.4 to 27.6 and from 26.5 to $34.5\%_{\circ}$, respectively. Range of pH, DO, COD, Chl-a, T-N and T-P were $7.82{\sim}8.39,\;5.31{\sim}11.28mg/L,\;0.13{\sim}1.38mg/L,\;3.05{\sim}11.55mg/L,\;0.005{\sim}0.180mg/L\;and\;0.007{\sim}0.028mg/L$ respectively. Dominant grain size was fine silt and the IL of sediment was ranged from $0.75{\sim}7.26%$. The water content was $20.16{\sim}53.65%$ and highest value was observed in Baegyado. The COD and AVS in the sediments ranged from $0.53{\sim}8.67mg/g-dry$ and $0.002{\sim}0.113mg/g-dry$ respectively. The bottom condition of Baegyado was higher than other area.

The Optimal Environmental Ranges for Wetland Plants: II. Scirpus tabernaemontani and Typha latifolia

  • Lee, Bo-Ah;Kwon, Gi-Jin;Kim, Jae-Geun
    • Journal of Ecology and Environment
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    • v.30 no.2
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    • pp.151-159
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    • 2007
  • We studied the optimal ranges of water and soil characteristics for wetland plants, particularly Scirpus tabernaemontani (softstem bulrush) and Typha latifolia (broadleaf cattail), which are dominant species with potential for restoration of Korean wetlands. We observed vegetation in S. tabernaemontani and T. latifolia communities from the mid to late June, 2005, and measured characteristics of water environments such as water depth (WD), temperature (WT), conductivity (WC), and concentration of several ions $(NO_3{^-}-N,\;Ca^{2+},\;Na^+,\;Mg^{2+},\;and\;K^+)$, and characteristics of soil environments such as soil texture, organic matter (loss on ignition, LOI), conductivity, and pH. The S. tabernaemontani community was accompanied by Zizania latifolia (Manchurian wildrice), Persicaria thunbergii (Korean persicary), Actinostemma lobatum (lobed actinostemma), and Beckmannia syzigachne (American slough grass), while the T. latifolia community was accompanied by P. thunbergii, T. angustifolia (narrowleaf cattail), and Glycine soja (wild soybean). We defined the optimal range for distribution (ORD) as the range that each plant was crowded. The optimal range of water characteristics for the S. tabernaemontani community was a $WD\;10{\sim}50cm,\;WT\;24.0{\sim}32.0^{\circ}C,\;WC\;100{\sim}500{\mu}S/cm,\;{NO_3}{^-}-N\;0{\sim}60ppb,\;K^+\;0.00{\sim}1.50ppm,\;Ca^{2+}\;7.50{\sim}17.50ppm,\; Na^+\;2.50{\sim}12.50ppm,\;and\;Mg^{2+}\;3.00{\sim}7.00ppm$. In addition, the optimal range of soil characteristics for the S. tabernaemontani community was a soil texture of loam, silty loam, and loamy sand, $LOI\;8.0{\sim}16.0%,\;pH\;5.25{\sim}6.25$, and conductivity $10{\sim}70{\mu}S/cm$. The optimal range of water characteristics for the T. latifolia community was a $WD\;10{\sim}30cm,\;WT\;22.5{\sim}27.5^{\circ}C,\;WC\;100{\sim}400{\mu}S/cm,\;{NO_3}{^-}-N\;0{\sim}60ppb,\;K^+\;0.00{\sim}1.50ppm,\;Ca^{2+}\;0.00{\sim}17.50ppm,\;Na^+\;0.00{\sim}12.50ppm,\;and\;Mg^{2+}\;0.00{\sim}5.00ppm$, and the optimal range of soil characteristics for the T. fatifolia community was a soil texture of loam, sandy loam, and silty loam, LOI $3.0{\sim}9.0%,\;pH\;5.25{\sim}7.25$, and conductivity $0{\sim}70{\mu}S/cm$.

Comparison of Flavor-related Components of Polygonatum odoratum in Root by Growth Year (둥글레 근경의 연근별 향미관련 성분의 비교)

  • Park, Nan-Young;Jeong, Yong-Jin;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.1
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    • pp.28-32
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    • 2003
  • As part of studies on the high quality dunggule tea, made of Polygonatum odoratum root with different growth year were analyzed for their flavor compounds. The quality attributes related to flavors were compared between raw and roasted samples which were treated at $130^{\circ}C$ for 15 min. Free sugars in the roots were mainly composed of fructose ($3.59{\sim}4.83%,\;d.b.$), sucrose ($1.78{\sim}2.49%$), and glucose ($1.60{\sim}1.80%$). Threonine ($1,239{\sim}1,444\;mg/100g,\;d.b.$), arginine ($589{\sim}689$), histidine ($289{\sim}370$) were three major free amino acids. The root grown for three years showed the highest content in total free sugar and amino acids (p<0.05). There was negligible difference in proximate composition and Hunter's color parameter depending on the growth year of the roots. However, sensory scores on color, taste and aroma were highest in 3 years' root, which is found most suitable for the preparation of dunggule tea. The roasting of 3 years old root significantly reduced the content of fructose and sucrose (p<0.01). It caused most apparent changes in the decreasing amount of threonine and in the decreasing rate of lysine, showing the significant decrease in total free amino acid content (p<0.01).

Kinetic Study on the Color Deterioration of Crude Anthocyanin Extract from Schizandra Fruit (Schizandra chinensis fructus) (오미자 색소 추출물의 가열 변색에 대한 속도론적 연구)

  • Cho, Sung-Bin;Kim, Hyun-Jung;Yoon, Jong-Il;Chun, Hyang-Sook
    • Korean Journal of Food Science and Technology
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    • v.35 no.1
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    • pp.23-27
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    • 2003
  • The effects of temperature and pH on color deterioration of anthocyanin in Schizandra fruit (Schizandra chinensis fructus) were determined with temperature range of $80{\sim}100^{\circ}C$ and pH range of $2.0{\sim}5.0$. Browning index was used as an index of color deterioration of crude anthocyanin extract from Schizandra fruit. As pH of crude anthocyanin extract was increased, color deterioration was accelerated, showing pH-dependency of thermal stability of anthocyanin extract from Schizandra fruit. Anthocyanin degradation could be modeled as a second-order rate reaction, with rate of $3.2{\times}10^{-3}\;h^{-1}\;(pH\;2.0){\sim}4.1{\times}10^{-3}\;h^{-1}\;(pH\;5.0)\;at\;100^{\circ}C$. Temperature dependence of deterioration was described by the Arrhenius relationship. Activation energies for pH $2.0{\sim}5.0$ ranged from $24.87{\sim}42.54\;kJ/mol^{-1}$.