• 한국환경보건학회지
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• 제18권2호
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• pp.67-74
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• 1992

The lab-scale biological A$_{2}$O system was applied from treating piggery wastewater highly polluted organic material which nitrogen and phosphorous are much contained relatively in conversion with other wastewater. The objective of this study was to investigate the effect of variance parameters on the treatability of this system according to operation conditions. An obtained experimental data were analysed by using principal component analysis (PCA) method. The results are summarized as follows: 1. From Varimax rotated factor loading in raw wastewater, variance of factor 1 was 36.8% and cumulative percentage of variance from factor 1 to factor 4 was 81.5% and of these was related to BOD, TKN and BOD loading. 2. In anaerobic process, variance of factor 1 was 33.5% and cumulative percentage of variance from factor I to factor 4 was 81.8% and of these was related to PO$_{4}$-P, BOD, DO and Temperature. 3. In anoxic process, variance of factor 1 was 30.1% and cumulative percentage of variance from factor i to factor 4 was 84.3% and of these was related to pH, DO, TKN and temperature. 4. In aerobic process, variance of factor 1 was 43.8% and cumulative percentage of variance from factor 1 to factor 4 was 81.5% and of these was highly related to DO, PO$_{4}$-P and BOD. 5. It was better to be operated below 0.30 kg/kg$\cdot$day F/M ratio to keep over 90% of BOD and SS, 80% of TKN, and 60% of PO$_{4}$-P in treatment efficiencies. 6. Treatment efficiencies was over 93% of BOD and SS, 81% of TKN and 60% of PO$_{4}$-P at over 20$^{\circ}$C, respectively.

• 한국한의학연구원논문집
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• 제16권1호
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• pp.119-134
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• 2010

Objectives & Method：Based on the statistic from January 2008 to December 2008 include 66-year-old 375 people (159 men, 216 women) who got examined in National Health Insurance Corporation, this study set a goal to establish a new health index by analyzing the relation between the cardio-cerebra vascular disease and risk factor such as obesity, smoking, exercise, alcohol consumption, blood pressure, blood sugar and cholesterol. Results：1) Examine results, health status according to gender；People who are diagnosed as abnormal health status have higher risk for both men and women. As for the detailed diseases, high blood pressure was highest followed by hyperlipidemia and obesity. In case of the women, 96.3% in bone mineral density was highest. 2) Correlation of disease and health risk factors based on gender；Correlation of disease according to gender in the obesity(p<0.001), lipid abnormalities(p<0.001), kidney diseases(p<0.001), chest diseases(p<0.05), osteoporosis(p<0.001) showed a statistically significant difference. The analysis showed that the men have more obesity and lipid abnormalities as well as thoracic disease than the women, but the women have more kidney disease than the men. As for the osteoporosis examine which is conducted only for the women, most of the women were abnormal even though it cannot compare the result according to gender. Obesity(p<0.001), alcohol(p<0.001), smoking(p<0.001) and LDLcholesterol(p<0.001) showed statistical correlation between gender and health risk factor. And only high blood pressure(p<0.001) showed a statistical correlation between gender and risk of cardio-cerebra vascular disease. 3) Risk of cardio-cerebra vascular disease related to health risk factor characteristic；Obesity assessment(p<0.001), alcohol(p<0.05), smoking(p<0.05), blood pressure(p<0.001), glucose(p<0.001) showed correlation between health risk factor and stroke. Drinking(p<0.001), smoking(p<0.05), exercise(p<0.001), blood pressure(p<0.001), LDL cholesterol(p<0.001) showed health risk factor and myocardial infarction. Obesity assessment(p<0.001), smoking(p<0.05), blood pressure(p<0.001), glucose(p<0.001) and showed correlation between health risk factor and diabetes. Obesity assessment(p<0.001), blood pressure(p<0.001) showed correlation between health risk factor and high blood pressure. Conclusion：Therefore, we have to take one step farther after the health examination. In order to prevent and reduce the risk of disease, people have to understand their health status exactly first, and correct their habit with recognizing the amount of risk related to cardio-cerebra vascular disease.

• Journal of Microbiology
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• 제45권6호
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• pp.534-540
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• 2007

The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor ${\sigma}^{HrdB}$ ($E{\cdot}{\sigma}^{HrdB}$) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme ($E{\cdot}{\sigma}^{HrdB}$). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for ${\sigma}^{HrdB}$ recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.

• 한국조리학회지
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• 제19권1호
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• pp.70-84
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• 2013

본 연구는 서울지역의 외식업체에서 소믈리에 서비스를 받아본 고객을 대상으로 소믈리에 서비스품질이 고객의 감정반응과 재방문의도에 미치는 영향에 대해 고찰하였으며, 소믈리에의 서비스품질 요인을 파악하고, 소믈리에의 서비스품질에 따른 고객의 감정반응이 재방문의도에 미치는 영향을 분석하여 소믈리에의 와인에 대한 전문지식, 기술과 경험에 대한 필요성을 제시하였다. 실증연구를 수행하기 위해 확보된 198개의 표본을 바탕으로 탐색적 요인분석, 신뢰도 분석, 회귀분석을 사용하여 총 3개의 가설을 검증하였다. 연구결과, 소믈리에 서비스품질이 긍정적 감정에 미치는 영향은 전문성 요인(${\beta}$=.257, p<0.001), 신뢰성 요인(${\beta}$=.314, p<0.001)과 대응성 요인(${\beta}$=.387, p<0.001)에서 유의한 영향을 미치는 것으로 나타났으며, 부정적 감정에 미치는 영향은 전문성 요인(${\beta}$=-.178, p<0.05)에서 유의한 영향을 미치는 것으로 나타났다. 소믈리에 서비스품질이 재방문요인에 미치는 영향을 분석한 결과는 신뢰성 요인(${\beta}$=.286, p<0.001)에서 유의한 영향을 미치는 것으로 나타났다. 고객의 감정반응이 재방문 요인에 미치는 영향을 분석한 결과는 긍정 감정요인(${\beta}$=.350, p<0.001), 부정 감정요인(${\beta}$=-.195, p<0.01)에서 유의한 영향을 미치는 것으로 나타났다. 이러한 연구결과를 통해 전문가로써의 소믈리에 자질향상을 통한 고객의 긍정적인 감정반응을 도출하여 재방문을 유도할 수 있는 방안을 제시하였으며, 연구의 한계 및 향후 연구방향에 대해서도 논의하였다.

• 대한의생명과학회지
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• 제2권2호
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• pp.231-240
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• 1996

인체 혈액 응고 9인자는 간에서 생성되며 461개의 아미노산으로 구성된 당 단백질이다. 따라서 인체 혈액 응고 9인자 cDNA를 찾기 위해 태아의 간(fetal liver) cDNA library를 PCR(Polymerase Chain reaction) 방법으로 screening하였으며, 그 결과 ATG개시 코돈으로부터 TAA종료 코돈까지 포함하는 1.4 kb의 9인자 cDNA를 찾았다. 또한 클론된 9인자 cDNA를 박테리아에서 발현시키기 위해 박테리아 발현 벡터인 pGEX-2T 플라스미드에 클로닝하므로써 pGEX-F9 플라스미드를 제조하였다. pGEX-F9로 형질전환된 E. coli에서 PGEX-F9의 발현을 유도하면 73 kDa 크기의 GST-factor9 융합 단백질이 다량생성되며 , 이 단백질이 혈액 응고 9인자 단백질을 함유하는 융합 단잭질임을 혈액 응고 9인자 항체를 이용한 Western blot으로 입증하였다. E. coli에서 발현된 GST-factor 9 융합 단백질은 전체 단백질의 약 20%를 차지하며 GST agarose bead를 이용한 one step purificarion 방법을 통해 GST-factor9 융합 단백질을 쉽게 분리 할 수 있다.

• 대한지역사회영양학회지
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• 제4권1호
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• pp.95-102
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• 1999

This study used questionnaires to investigate the safety awareness for imported foods by 365 male and female adults in Taejon. The results of the study were as follow : By factor analysis, the subjects' behaviors and awareness of the imported food was grouped into 3 factors such as 'health and quality factor', 'purchasing factor' and 'contamination factor'. 'Health and quality factor' and 'purchasing factor' were not recognized negatively by the subjects, moreover' contamination factor' was recognized very highly. The subjects' concern and worry about the imported food was also very high. The marital status, education level, nutriton knowledge adn recognition level of contamination by pesticides and heavy metals of foods partially affected the recognitio level of imported foods. The major selection criteria of imported food were distribution period(36.3%), price(28.8%) and purchasing experience(17.3%). The imported food mean discrimination score was 8.4±3.1 out of 13. The worst discriminatio score was red pepper. The subjects' experiences with imported foods selection affected the most instead of education level or nutrition knowledge. The higher discrimination score group more negatively recognized imported food and contamination recognition level was higher whereas the lowerdiscrimination score group more positively recognized the purchasing frequency and with to buy more easily. But both groups desired to reinforce contamination control. The better discrimination score of imported food pooring recognized sanitation concerns(p<0.05), quality(p<0.05), cooking convenience(p<0.01), desire for more variety(p<0.05), and the higher contamination recognition level(p<0.05) and desire to reinforce contamination control(p<0.01).

• Structural Engineering and Mechanics
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• 제12권3호
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• pp.249-266
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• 2001

Current seismic design provisions allow structures to deform into inelastic range during design level earthquakes since the chance to meet such event is quite rare. For this purpose, design base shear is defined in current seismic design provisions as the value of elastic seismic shear force divided by strength reduction factor, R (${\geq}1$). Strength reduction factor generally consists of four different factors, which can account for ductility capacity, overstrength, damping, and redundancy inherent in structures respectively. In this study, R factor is assumed to account for only the ductility rather than overstrength, damping, and redundancy. The R factor considering ductility is called "ductility factor" ($R_{\mu}$). This study proposes ductility factor with correction factor, C, which can account for dynamic P-${\Delta}$ effect. Correction factor, C is established as the functional form since it requires computational efforts and time for calculating this factor. From the statistical study using the results of nonlinear dynamic analysis for 40 earthquake ground motions (EQGM) it is shown that the dependence of C factor on structural period is weak, whereas C factor is strongly dependant on the change of ductility ratio and stability coefficient. To propose the functional form of C factor statistical study is carried out using 79,920 nonlinear dynamic analysis results for different combination of parameters and 40 EQGM.

• 혜화의학회지
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• 제22권1호
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• pp.201-216
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• 2013

The purpose of this case-control study was to show the relationship between risk factor(Sex, Age, Martial Status, Educational Periods, Family history, Variables of Obesity, Smoking Status, Drinking Status, Past History, Blood Test) and the incidence of stroke. Methods: 788 stroke patients were enrolled as the case group and 450 non-stroke patients as control group from Sep.2006 to Dec.2010. Patients were hospitalized within 30 days after the onset of stroke. Risk factors and warning signs were obtained from personal interview by oriental medicine doctors who used CRF. Results: 1. Age was found to have significant relation with stroke(p<.0001). 2. Martial status(p<.0001, OR=0.44) and educational periods(p<.0001) were both significant risk factor for stroke. 3. As a result of reviewing the influence of family history, it was found to have no significant relation with stroke. Stroke was found to have significant relation with stroke(OR=1.50). 4. Female waist circumference(WC, p<.0001) and female waist-hip circumference ratio(WHR, p<.0001) were risk factors for stroke. On the other hand, male WC and male WHR showed no significant influence on stroke occurrence as an independent risk factor. 5. Both smoking status(p<.0001, current smoker OR=8.95) and drinking status(p<.0001, current drinker OR=2.50, former drinker OR=2.82) were significant risk factors for stroke. 6. As a result of reviewing the influence of past history, transient ischemic attack(p<.0001, OR=8.46), hypertension(p<.0001, OR=4.72), hyperlipidemia(0.0064), diabetes mellitus(p<.0001, OR=3.34), stroke(p<.0001, OR=50.26) were significant risk factors. On the other hand, ischemic heart disease(p=0.2332) was found to have no significant relation with stroke. 7. Increase of WBC, RBC, Platelet, TG, FBS and Cl- level were significant risk factor of stroke, On the other hand, decrease of Hgb, Hct, AST, HDL-chol, LDH, Na+ and K+ level were significant risk factor of stroke. T-chol and ALT level showed no significant influence on stroke occurrence.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.506-512
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• 1995

A cell aggregation factor produced by Aspergillus sp. LAM 94-142 was purified and partially characterized. The factor was purified about 15 folds from culture broth by IRA 420 and IRC 120 treatment, 1% NaCl added acetone precipitation, and Sepharose 4B column chromatography with overall yield of 48%. It was heteropolysaccharide consisted of mannose, arabinose, and glucose with a molar ratio, 31:17:2, and its molecular weight was estimated to be about 900,000 daltons by Sepharodse 4B gel filtration method. The optimum pH and temperature was 8 and 40$\circ$C, respectively. The factor was stable in pH range of 3-9 and at 100$\circ$C for 90 min. The cell aggregation activity of the factor was inhibited by the addition of Hg$^{2+}$, Fe$^{2+}$, Cu$^{2+}$, and some polypeptides such as milk casein or hemoglobin. The factor aggregated Bacillus subtilis, B. macerans, B. turingiensis, E. coli, Peudomonas aeruginosa, P. fluorescens, P. malophilia, and weakly aggregated Staphylococcus sp., Sarcina lutea, P. putida and Cryptococcus neoformnans, but it didn't aggregate various strains of Candida sp. and Saccharomyces sp.

• 한국응급구조학회지
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• 제12권2호
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• pp.85-97
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• 2008

Purpose : This study was cross-sectional study to examine the influence of job stress in 119 briefing room workers and aimed to provide the important basic materials for improving working conditions of 119 briefing room workers and further studies. Methods : This study selected 271 workers of 119 briefing room at National Emergency Management Agency and fire stations, metropolitan cities and provinces to examine the influences of job stress of 119 briefing room workers on depression. and as a result of analysis, the following conclusions. Results : 1) The whole average of job stress measure was 3.30. The highest area was interpersonal relationship factor as 3.41, 'job characteristic factor' as 3.36 and communication factor' as 3.01. 2) Total score of whole items of depression was 11.88 and showed slight depression level. 3) There was static correlation between job stress and depression(r=44.7, p= .000). There were statistically significant static correlations between job stress factor and depression(r= .388, p= .000), interpersonal relationship factor and depression(r= .471, p= .000), and communication factor and depression(r= .386, p= .000). 4) There was statistically significant difference in the influence of job stress on depression(B=8.575, p< .001). Influence of job stress on depression was explained as $R^2=.200$. When job stress factors as independent variables were input, interpersonal relationship factor(B=5.062, p< .001) had the greatest influence on depression and there was no statistically significant difference in communication factor(B=2.490, p< .05). Conclusions : It is considered that development of the various programs including program for getting rid of job stress of 119 briefing room workers and counseling program will be needed. particularly, factors such as human relationships and communication in the area of job stress must be the first priority.