• Title/Summary/Keyword: P%26I

Search Result 1,345, Processing Time 0.037 seconds

Subcloning of Nodulin 26 Wild Type(S262) and Phosphorylation Site Mutant(S262D) into the Yeast Expression Vector pYES2

  • Cha, Youn-Soo
    • Preventive Nutrition and Food Science
    • /
    • v.2 no.1
    • /
    • pp.61-65
    • /
    • 1997
  • Wild type nodulin 26(nod 26) cDNA(S262) and phodphorylation aite mutant(S262D) were constructed by a yeast expression system using pYES2 plasmids(pTES2-D262 and pTES2-S262D) were sc-reened by restriction mapping with BamHI of KpnI. S262 nod 26 contained a sreine residue at position 262 and S262D nod 26 contained the substitution mutation of serine to aspartic acid residue at position 262 were verified by automated floursent DNA sequencing.

  • PDF

OSCILLATION OF ONE ORDER NEUTRAL DIFFERENTIAL EQUATION WITH IMPULSES

  • Cheng, Jinfa;Chu, Yuming
    • Communications of the Korean Mathematical Society
    • /
    • v.26 no.2
    • /
    • pp.197-205
    • /
    • 2011
  • Explicit sufficient conditions are established for the oscillation of the one order neutral differential equations with impulsive $(x(t)+{\sum\limits^n_{i=1}}c_ix(t-{\sigma}_i))'+px(t-{\tau})=0$, $t{\neq}t_{\kappa}$, ${\Delta}(x(t_{\kappa})+{\sum\limits^n_{i=1}}c_ix(t_{\kappa}-{\sigma}_i))+p_0x(t_{\kappa}-{\tau})=0$, $c_i{\geq}0$, $i=1,2,{\ldots}n$, $p{\tau}$>0, $p_0{\tau}$>0, ${\Delta}(x_{\kappa})=x(t^+_{\kappa})-x(t_{\kappa})$. Explicit sufficient and necessary condition are established when $c_i$ = 0, i = 1, 2, ${\ldots}$, n.

Effects of Concentrate Feeding Method and Slaughter Age on Growth Performance, Feed Intake and Carcass Characteristics of Hanwoo Steers (거세한우 배합사료 급여방법 및 출하월령이 발육, 사료섭취량 및 도체특성에 미치는 영향)

  • Cho, Young-Moo;Chang, Sun-Sik;Kim, Hyeong-Cheol;Kim, Tae-Il;Park, Byung-Ki;Paek, Bong-Hyun;Kim, Jin-Hyoung;Kwon, Eung-Gi
    • Journal of Animal Science and Technology
    • /
    • v.51 no.1
    • /
    • pp.53-60
    • /
    • 2009
  • Thirty one Hanwoo steers were used in a completely randomized design experiment to see the effects of concentrate feeding method (group vs. individual feeding) and age at slaughter (26 vs. 28 months of age) on growth performance, feed intake and carcass characteristics. Steers were randomly assigned to one of four groups, G26 (group feeding slaughtered at 26 months of age), G28 (group feeding slaughtered at 28 months of age), I26 (individual feeding slaughtered at 26 months of age) or I28 (individual feeding slaughtered at 28 months of age). Amount of concentrate were restricted to 1.5% of BW for the growing period and 1.8% of BW for the early fattening period in both groups. For the late fattening period, concentrate were given ad libitum for G26 and G28, but were given up to 1.7% of BW for I26 and I28. Average daily gains were higher in individual feeding groups (I26 and I28) than those in group feeding groups (G26 and G28) for the early fattening period (p<0.05). ADGs were higher in group feeding groups than those in individual feeding groups (p<0.05). Concentrate intake in individual feeding groups tended to be higher compared to that in group feeding groups for the early fattening period, but tended to be higher in group feeding groups compared to that in individual feeding groups for the late fattening period. Feeding method or slaughter age had no significant effects on carcass characteristics, yield grade and quality grade. Shear force and tenderness of longissmus muscle were higher in groups slaughtered at 28 months of age than in groups slaughtered at 26 months of age (p<0.05). Feeding method or slaughter age had no effects on cooking loss, water holding capacity, juiciness, flavor, moisture, protein and fat of longissmus muscle.

Fixing Behaviors of Dimethylamino Anthraquinone Disperse Dyes and Monochlorotriazinyl Azo Reactive Dyes on P/C Blended Fabrics in One-Step Printing (디메틸아미노안트라퀴논계 분산염료와 아조계 모노클로로트리아진형 반응염료에 의한 P/C혼방직물의 일단계 날염에 있어 고착거동)

  • Park, Geon-Yong;Seo, Gi-Sung
    • Textile Coloration and Finishing
    • /
    • v.19 no.3
    • /
    • pp.18-25
    • /
    • 2007
  • The fixing behaviors of anthraquinone disperse dyes containing dimethylamino substituent, such as C. I. Disperse Violet 26(D.V.26) and C. I. Disperse Blue 14(D.V.14), or containing diamino substituent, such as C. I. Disperse Blue 73(D.B.73), and monochlorotriazinyl azo reactive dyes, such as C. I. Reactive Orange 13(R.O.13), C. I. Reactive Red 3(R.R.3). C. I. Reactive Yellow 2(R.Y.2) on polyester/cotton blend(P/C) fabrics were examined for the one-step printing of P/C fabrics. The high temperature steaming of $175^{\circ}C$ is the most satisfactory fixing method for P/C one-step printing with above disperse and reactive dyes among the four different fixing methods: $175^{\circ}C$ steaming, $102^{\circ}C$ steaming${\rightarrow}175^{\circ}C$ steaming, $190^{\circ}C$ thermosol, $102^{\circ}C$ steaming${\rightarrow}190^{\circ}C$ thermosol. $190^{\circ}C$ thermosol is unfit to fix R.R.3 and R.Y.2 whose heat stability is poor. It was found that D.V.26 and D.B.14 containing dimethylamino substituent are unstable for heat and alkali, but D.B.73 is stable for them to print P/C blend fabrics with R.O.13 which is also stable for heat. Therefore we found that D.B.73, R.O.13 and a pair of D.B.73 and R.O.13 were very suitable for one-step printing of P/C blend fabrics.

Changes of Mortality and Morbidity of Very Low Birth Weight Infants after Neonatal Intensive Care Unit Strategy Alteration in a Single Center: Comparison with 2015 Korean Neonatal Network Report

  • Jung, Seung Mi;Seok, Min Jeong;Chun, Ji Yong;Sung, Tae-Jung
    • Neonatal Medicine
    • /
    • v.25 no.1
    • /
    • pp.29-36
    • /
    • 2018
  • Purpose: The purpose of this study was to investigate the outcome after changes in the treatment strategies for very low birth weight infant (VLBWI) in a single neonatal intensive care unit (NICU) center. Methods: We performed a retrospective review of 300 VLBWI born from 1st January 2010 to 31th December 2016. We compared the outcomes including survival rate, birth weight (BW), gestational age (GA), and morbidities between period I (2010-2013, P-I) and period II (2014-2016, P-II). Results: The average survival rate was not different between P-I and P-II. However, the survival rate of ${\leq}24$ weeks' GA, 25 weeks' GA, 26 weeks' GA were 57%, 69%, 93% respectively in P-II and 31%, 59%, 87% in P-I respectively. The survival rate of infants with birth weight <500 g, 500-749 g, 750-999 g were 100%, 55%, 90% respectively in P- II and 50%, 24%, 80%, respectively in P-I. The incidence of bronchopulmonary dysplasia (BPD) was higher in P-II than in P-I (P=0.012) and moderate-to-severe BPD was also higher in P-II (P=0.004). Incidence of patent ductus arteriosus (PDA) with treatment, necrotizing enterocolitis (stage ${\geq}2$), and abnormal brain sonography were significantly lower in P-II (P=0.027, P=0.032, P=0.005). Incidences of retinopathy of prematurity (ROP) with laser treatment and early sepsis were not different. Conclusion: The survival rate and complications of VLBWI were improved in period II, especially in less than 750 g and below 26 weeks, except incidence of BPD. Changes of NICU strategies were effective to improve mortality and morbidity in VLBWI.

Genetic Polymorphism of Cytochrome P450 2E1 in Korean Population (한국인 집단에서 Cytochrome P450 2E1의 유전적 다형성)

  • 정혜광;구희경;이상섭;양규환;정태천;변부형
    • Environmental Mutagens and Carcinogens
    • /
    • v.16 no.2
    • /
    • pp.97-102
    • /
    • 1996
  • In this study, we have quantified genotype frequency of the cytochrome P450 (P450) 2E1 which codes for the P450 enzyme primarily responsible for the metabolic activation of carcinogenic nitrosamines and low molecular organic solvents, in Korean population by using PCR and RFLP at two sites previously associated with some cancers; a PstI and RsaI RFLP in the transcriptional regulatory region of the human P450 2E1 gene. The genotype frequencies of homozygous wild type (PstI site-absent) and heterozygous mutant type (PstI site-present) in PstI RFLP were 0.70 and 030, respectively. The homozygous mutant type in Pstl RFLP was not observed in Korean population. The genotype frequencies of homozygous wild type (RsaI site-present), heterozygous mutant type (RsaI site-absent), and homozygous mutant type in RsaI RFLP were 0.71, 0.26, and 0.03, respectively.

  • PDF

The Present Situation and Problems of In Vitro Fertilization in Swine (돼지 체외수정의 현황과 문제점)

  • 류일선
    • Journal of Embryo Transfer
    • /
    • v.7 no.1
    • /
    • pp.41-47
    • /
    • 1992
  • 1. In vitro system, LR and FSR accelerated and facilitated meiotic progression, and LH selectively improved cytoplasmic maturation which is required to promote the formation of a male pronucleus. 2. Caffeine (2mM) in the fetilization medium was required not only for inducing zona penetrating ability of boar also for developing to the male pronucleus of the penetrat- ing spermatozoa in vitro. 3. The germinal vesicle (GV)stage was observed for the first 17.6 hr;germinal vesicle break-down (GVBD)stage between 17.6~26.4 hr ;metaphase I (M-I)from 26.4 - 30. 9hr;anaphase I(A-I)ranged from 30. 9~33.4hr;telophase I(T-I) at 33.4~34.4hr; and metaphase II(M-II) at 34.4-48hr. 4. The addition of 10%(v /v) pig follicular fluid (pFF) to maturation media significantly increased the rate of nuclear maturation of pig oocytes (p<0.01), whereas the rate of nuclear maturation of pig oocytes among three different media did not differ. 5. The presence of a primary culture of POEC promotes in vitro development of early cleavage stage pig embryos.

  • PDF

The Result of Combined Modality Treatment for Non-Hodgkin's Lymphoma of Head and Neck (두경부 악성 임파종에 대한 병용치료의 결과)

  • Kim Jae Cheol;Kim Sang Bo;Ryu Samuel;Park In Kyu
    • Radiation Oncology Journal
    • /
    • v.8 no.2
    • /
    • pp.255-260
    • /
    • 1990
  • From April 1985 to September 1989, 26 patients with stage I and II non-Hodgkin's lymphoma of unfavorable histology localized in head and neck region were treated with combined modality (combination chemotherapy plus radiotherapy) at the Department of Therapeutic Radiology in Kyungpook National University Hospital. Of the 26 patients, 23 showed complete response and 3 partial response. Between these two groups there were no statistical differences according to the variables. Three-year survival and disease-free survival rate were $62.4{\%}$ and $65.2{\%}$, respectively. Unilateral involvement of neck node (p<0.05), radiation dose over 5000 cGy (p<0.01), and 6 or more cycles chemotherapy (p=0.06) had a favorable effect on 3-year survival rate. There were 8 recurrences including 3 partial responders, 1 local failure, 1 distant failure, 1 contiguous failure, and 2 simultaneous local and distant failure. It could be suggested that combined modality treatment might be necessary for the treatment of stage I and II Non-Hodgkin's lymphoma of unfavorable histology.

  • PDF

Characteriaation of BmaI methylase from bacillus macerans (Bacillus macerans의 BmaI methylase의 특성)

  • 권용태;전희숙;노현모
    • Korean Journal of Microbiology
    • /
    • v.26 no.2
    • /
    • pp.88-92
    • /
    • 1988
  • The isolation and characterization of a new type II methylase, BmaI methylase, from Bacillus macerans ATCC 8244 were described. BmaI methylase was isolated by procedures of ammonium sulfate fractionation, DEAE-cellulose chromatography and phosphocellulose chromatography. Two types of methylases were present in this strain and only one of the two was a site specific BmaI methylase. The pBR322 DNA methylated by BmaI methylase was not cleaved by BmaI endonuclease, and pBR322 DNA cleaved by BmaI endonuclease was not methylated by BmaI methylase. The optimal pH for the BmaI methylase activity was 7.5, and optimal NaCl concentration was about 50 mM. BmaI methylase could methylate single-stranded M13mp18 DNA.

  • PDF

Studies on the Characteristics of Phosphorus in the Upland Soil I. Composition of Accumulated Phosphorus Forms and Available Phosphorus (경작지(耕作地) 전토양(田土壤)의 인산특성(燐酸特性)에 관(關)한 연구(硏究) I. 축적인산(蓄積燐酸)의 형태별(形態別) 조성(造成)과 유효인산(有效燐酸) 함량(含量)과의 관계(關係))

  • Shin, Cheol-Woo;Kim, Jeong-Je;Yoon, Jung-Hui
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.21 no.1
    • /
    • pp.21-29
    • /
    • 1988
  • A laboratory experiment was conducted to evaluate the phosphorus status and define the relationship between composition of phosphorus forms and available phosphorus with 149 phosphorus accumulated soil samples. Distribution percentage of inorganic, organic and available phosphorous to total phosphorus were 68.9 (Saloid-p 2.7, Al-p 26.4, Fe-p 27.6, Ca-p 12.2%), 6.7 and 26.0%, respectively. In the relationship between available phosphorus and inorganic phosphorus in soil, Al-p, Saloid-P, and Ca-P showed significant correlation with available phosphorus which did not showed significant one with Fe-p. Multiple liner regression equation between available and inoganic phosphorus abtained as follow, $Av.P=81.694+0.858Sa-p^{***}+0.648Al-p^{***}+0.091Ca-p^{**}(R=0.826^{***})$ and contribution rates of Saloid-p, Al-p, and Ca-p to available phosphorus were 26.1, 65.2, and 8.7%, respectively. Relationship between available and water soluble phosphorus, water soluble phosphorus and $0.01M-CaCl_2-p$ were highly significant, respectively, and soil solution P extracted with $0.01M-CaCl_2$ for 30 minutes was selected as one of usable diagnostic techniques for soil P status.

  • PDF