• Title/Summary/Keyword: Oxygen Consumption Rate

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Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents

  • Huang, Yun;Kwan, Kenneth Kin Leung;Leung, Ka Wing;Yao, Ping;Wang, Huaiyou;Dong, Tina Tingxia;Tsim, Karl Wah Keung
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.517-526
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    • 2019
  • Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used "Qi-invigorating" herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment. Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxideetreated live cultures was determined by Seahorse extracellular flux analyzer. Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage. Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration.

The Characteristics of Oxygen Deficient Water Mass in Gamak Bay (가막만 빈산소 수괴의 특성)

  • Kim, Jeong-Bae;Lee, Sang-Yong;Yu, Jun;Choi, Yang-Ho;Jung, Chang-Su;Lee, Pil-Yong
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.9 no.4
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    • pp.216-224
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    • 2006
  • To clarify the formation process and characteristics of oxygen deficient water mass in Gamak Bay, oxygen deficiency was weekly observed from 17 June to 12 September 2005. Surface water temperature was significantly lower in the outer bay than in the inner bay, whereas the bottom water temperature was higher in the central area of bay than in the outer and inner bay. The vertical stratification of water mass was strongly formed during the period, and thermocline was observed between 3 and 5m deep. The oxygen deficiency in the bottom layer began to appear at early July in the inner bay and gradually spread to the center area of the bay in early August. The mean transparency and light attenuation coefficient($K_d$) in water mass was 4.0m and 0.47, respectively. Average concentrations of nutrient and chlorophyll ${\alpha}$ in the bottom layer were significantly higher than those in surface, and those concentrations were significantly higher in the inner bay than in the outer bay. During the formation of oxygen deficiency in the bottom layer, oxygen penetration depth in the bottom sediment were extremely shallow, and oxygen consumption rate in the bottom sediment were lower than that in the area where oxygen deficient water mass disappeared. Dissolved oxygen concentrations in the bottom layer are negatively correlated with nutrient concentrations, whereas those in the surface layer did not show a significant relationship with nutrient concentrations. Elevated loss of oxygen in the bottom water mass was attributed to the increase of the oxygen consumption rates in sediments and the decomposition of organic matter by microorganism.

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Organic Carbon Cycling in Ulleung Basin Sediments, East Sea (동해 울릉분지 퇴적물에서 유기탄소 순환)

  • Lee, Tae-Hee;Kim, Dong-Seon;Khim, Boo-Keun;Choi, Dong-Lim
    • Ocean and Polar Research
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    • v.32 no.2
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    • pp.145-156
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    • 2010
  • This study investigated organic carbon fluxes in Ulleung Basin sediments, East Sea based on a chamber experiment and geochemical analyses. At depths greater than 2,000 m, Ulleung Basin sediments have high organic carbon contents (over 2.0%). Apparent sedimentation rates (ASR) calculated from excess $^{210}Pb$ activity distribution, varied from 0.036 to $0.047\;cm\;yr^{-1}$. The mass accumulation rates (MAR) calculated from porosity, grain density (GD), and ASR, ranged from 131 to $184\;g\;m^{-2}\;yr^{-1}$. These results were in agreement with sediment trap results obtained at a water depth of 2100 m. Input fluxes of organic carbon varied from 7.89 to $11.08\;gC\;m^{-2}\;yr^{-1}$ at the basin sediments, with an average of $9.56\;gC\;m^{-2}\;yr^{-1}$. Below a sediment depth of 15cm, burial fluxes of organic carbon ranged from 2.02 to $3.10\;gC\;m^{-2}\;yr^{-1}$. Within the basin sediments, regenerated fluxes of organic carbon estimated with oxygen consumption rate, varied from 6.22 to $6.90\;gC\;m^{-2}\;yr^{-1}$. However, the regenerated fluxes of organic carbon calculated by subtracting burial flux from input flux, varied from 5.87 to $7.98\;gC\;m^{-2}\;yr^{-1}$. Respectively, the proportions of the input flux, regenerated flux, and burial flux to the primary production ($233.6\;gC\;m^{-2}\;yr^{-1}$) in the Ulleung Basin were about 4.1%, 3.0%, and 1.1%. These proportions were extraordinarily higher than the average of world open ocean. Based upon these results, the Ulleung Basin might play an integral role in the deposition and removal of organic carbon.

External Attachment of Pop-up Satellite Archival Tag (PSAT) and Water Temperature Affect Oxygen Consumption Rate of the Olive Flounder Paralichthys olivaceus (넙치(Paralichthys olivaceus) 산소 소비율에 미치는 Pop-up Satellite Archival Tag (PSAT) 체외 부착과 수온의 영향)

  • Geun Su Lee;Pil Jun Kang;Hye Mi Park;Sung-Yong Oh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.5
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    • pp.660-666
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    • 2023
  • This study aimed to examine the effect of external pop-up satellite archival tags (PSATs) attachment and water temperature on the oxygen consumption rate (OCR) of the olive flounder (mean body weight 2281.7 g). The OCRs of fish were measured under conditions of three different water temperature conditions (15, 20, and 25℃) and two different tagging methods [non-tagging, control; bio-logger external attachment with a miniature PSAT (dummy mrPAT), BEA] using a closed flow-through respirometer. The OCRs of fish linearly increased with the increase in water temperature in both the control and BEA (P<0.001); however, the OCRs of BEA were approximately 1.8-1.9 times lower than those of the control at each water temperature (P<0.001). The Q10 values of the control and BEA were the highest in the water temperature range of 15 to 20℃, but sensitivity to water temperature changes was higher in BEA than in the control. The metabolic energy loss rate (MEL) of fish increased with increasing water temperature regardless of external tagging, but the MEL of the control was higher than that of BEA (P<0.001). These results demonstrate that OCR, thermal sensitivity, and energy expenditure are all affected in adult olive flounder with external PSAT attachment.

The Study of a Correlation between Heat Release and Smoke Production by Using Oxygen Consumption Calorimeter Up to 10 MW Facility (10MW급 까지의 산소소모율법 칼로리미터를 활용한 열방출률과 연기발생률의 상관성에 관한 연구)

  • Ryu, Sang-Hoon;Yoo, Yong-Ho;Rie, Dong-Ho
    • Fire Science and Engineering
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    • v.24 no.3
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    • pp.58-65
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    • 2010
  • The fire accidents having recently occurred are getting more and more larger and causing lots of damage in terms of property loss and casualties increase, so there is in need of technical fire safety development like comprehensive prevention solution in order to effectively prevent. Especially, the needing of new paradigm for advanced fire safe technology is gathering strength in high-rise modern building construction. Therefore, we want to find out on this paper what is a correlation between heat release rate measurement and smoke release volume by three parts of oxygen consumption calorimeter in bench scale calorimeter (cone calorimeter/ISO 5660/Avg.500Kw), Medium scale calorimeter (Room corner tester, Single burning Item/ISO 9750, EN 13823/Avg.3MW), and large scale calorimeter (Industry calorimeter/Avg. 10MW). Thus, Smoke detective of new paradigm devised by making use of a correlation between heat release and smoke production is to help reduce loss property and casualties. Ultimately, based on this theory, a new concept of fire alarm and evacuation system will be developed and expected to apply to a skyscraper.

Effects of water temperature changes on oxygen consumption and hematological factors in olive flounder Paralichthys olivaceus

  • Lim, Han Kyu;Han, Hyon Sob;Hur, Jun Wook
    • Fisheries and Aquatic Sciences
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    • v.24 no.2
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    • pp.99-107
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    • 2021
  • Water temperature (WT) is a major environmental factor of metabolic rate in fish; it directly affects food intake, ammonia excretion, oxygen consumption (OC), growth, and survival. Thus, the purpose of this study was to investigate changes in the OC and the hematological response of olive flounder Paralichthys olivaceus because of WT changes. In Exp. I, WT was increased from 20℃ to 29℃ within 18 h and maintained at 29℃ for 96 h. Then, WT was decreased from 29℃ to 20℃ within 18 h and maintained at 20℃ for 24 h. In Exp. II, WT was decreased from 20℃ to 11℃ within 18 h and maintained at 11℃ for 96 h. Then, WT was increased from 11℃ to 20℃ within 18 h and maintained at 20℃ for 24 h. The Exp. III maintained that the Exp. I and II was consecutively. In Exp. I, the OC increased from 116.7 mg O2 kg-1 hr-1 to 317.5 mg O2 kg-1 hr-1 with increasing WT. In Exp. II, the OC decreased from 96.5 mg O2 kg-1 hr-1 to 71.3 mg O2 kg-1 hr-1 with decreasing WT. In Exp. III, OC tended to increase or decrease in inverse proportion to temperature. In Exp. I, cortisol, glucose, and aspartate aminotransferase (AST) values increased with increasing WT. In Exp. II, Cl-, osmolality, AST, and alanine aminotransferase (ALT) values significantly changed during the experimental period: glucose values increased, whereas cortisol values decreased with decreasing WT. Exp. III was shown to be a more stressful environment to olive flounder than Exp. I and Exp. II. The results of our study will be useful for evaluating current aquaculture procedures of olive flounder and developing techniques to minimize stress in aquaculture farms.

Analysis of emergy comsumption for Hybrid Gait Orthosis (하이브리드 보행보조장치의 에너지 소모도 비교분석)

  • 강성재;류제청;김규석;문무성
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.814-817
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    • 2004
  • It is a challenging task to make the paraplegic to walk with out the assist of the caregiver. So, we have developed various type of gait orthosis for paraplegic during the five years lately. The purpose of this study ultimately is energy consumption test of serveral type gait orthosis for developing the high efficiency gait orthosis. From the experimental results, the oxygen consumption rate were 6.9$\pm$3.3ml/kg in RGO gait, 5.3$\pm$1.3ml/kg in PGO, and 6.2$\pm$3ml/kg in HGO gait. The maximum hip flexion angle were 16$^{\circ}$ in RGO , 15$^{\circ}$ in PGO, and 47.5$^{\circ}$. in HGO. As a result It was found that. Hybrid Gait Orthosis need high energy consumption more than PGO for walking, but it is small weight and strengthened muscle.

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Environmental Impact Assessment by Marine Cage Fish Farms: II. Estimation of Hydrogen Sulfide Oxidation Rate at $O_2$-H$_2$S Interface and Sulfate Reduction Rate in Anoxic Sediment Layer (해상 어류가두리양식장의 환경영향 평가: II. 가두리 양식장 퇴적물의 산소-황화수소 경계면에서 황화수소의 산화율 및 무산소 퇴적층에서 황산염 환원율 추정)

  • Lee, Jae-Seong;Kim, Kee-Hyun;Yu, Jun;Lee, Pil-Yong;Jung, Rae-Hong;Lee, Wong-Chan;Han, Jung-Jee;Lee, Yong-Hwa
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.9 no.2
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    • pp.64-72
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    • 2004
  • We measured the vertical profiles of $O_2$, H$_2$S, and pH in sediment pore water beneath marine cage fish farms using a microsensor with a 25 ${\mu}{\textrm}{m}$ sensor tip size. The sediments are characterized by high organic material load. The oxygen consumption, hydrogen sulfide oxidation, and sulfate reduction rates in the microzonations (derived from the vertical distribution of chemical species concentration) were estimated by adapting a simple one-dimensional diffusion-reaction model. The oxygen penetration depth was 0.75 mm. The oxic microzonations were divided into upper and lower layers. Due to hydrogen sulfide oxidation within the oxic zone, the oxygen consumption rate was higher in the lower layer. The total oxygen consumption rate integrated with reaction zone depth was estimated to be 0.092 $\mu$mol $O_2$cm$^{-2}$ hr$^{-1}$ . The total hydrogen sulfide oxidation rate occurring within 0.7 mm thickness was estimated to be 0.030 $\mu$mo1 H$_2$S cm$^{-2}$ hr$^{-1}$ , and its turnover time in the oxic sediment layer was estimated to be about 2 minutes. This suggests that hydrogen sulfide was oxidized by both chemical and microbial processes in this zone. The molar consumption ratio, calculated to be 0.84, indicates that either other electron accepters exit on hydrogen sulfide oxidation, or elemental sulfur precipitation occurs near the $O_2$- H$_2$S interface. Total sulfate reduction flux was estimated to be 0.029 $\mu$mol cm$^{-2}$ hr$^{-1}$ , which accounted for more than 60% of total $O_2$ consumption flux. This result implied that the degradation of organic matter in the anoxic layer was larger than in the oxic layer.

An Evaluation of the Environmental Effects of Marine Cage Fish Farms: I. Estimation of Impact Region and Organic Carbon Cycling in Sediment Using Sediment Oxygen Consumption Rates and Macrozoobenthos (해상 어류가두리양식장의 환경영향평가: I. 퇴적물 산소소모율 및 저서동물을 이용한 유기물 오염영향권 추정 및 유기탄소 순환)

  • 이재성;정래홍;김기현;권정노;이원찬;이필용;구준호;최우정
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.9 no.1
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    • pp.30-39
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    • 2004
  • In order to understand the environmental impact of marine cage fish farms, we measured the vertical fluxes of particulate to the sediment, the distribution of organic carbon in core samples, sediment oxygen consumption rate (SOD), and macrobenthos with increasing distance from a fish cage in Miruk island located in Tongyong. The experiment was performed in August 2003. Measured values gradually decreased with distance, indicating that the organic matter in the sediment derived from the fish farm. The dominant macrobenthos species were Tharyx mulifilis, Lumbrineris longifolia, Sigambra tentaculata, and Capitella capitata, occupying 88% of the total population. Capirella capirata, an opportunistic polychaete species, were especially abundant between 0 to 5 m radius range. The estimated impact regions of organic matter enrichment based on sediment consilmption rates and compositions of macrobenthos were in good agreement. Most organic matter derived from the fish farm was deposited within a 10 m radius and then dispersed horizontally to nearby (at least 50 m) surface sediment. The vertical organic carbon fluxes to the sediment at the fish farm were higher by a factor of two than those outside the area. The remineralization organic carbon in the upper sediment layer was estimated to be 50% (1.07 g C m$^{-2}$ day$^{-1}$ ) at the fish farm. In contrast, outside the area, 30% (0.30 g C m$^{-2}$ day$^{-1}$ ) of organic carbon was recycled and the remaining 70% was deposited to the deep sediment layer.

Effects of the Experimental Vehicles on the Greenhouse Worker′s Work Load (비닐하우스 작업시 승용 농작업차의 노동부담 경감효과)

  • Choi, Jung-Hwa;Seol, Hyang;Ryu, Kwan-Hee
    • Korean Journal of Rural Living Science
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    • v.8 no.1
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    • pp.7-13
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    • 1997
  • In this study we examined the greenhouse worker's work load to test the efficiency of the developed vehicles (hand operated vehicle (HV), simple battery-powered autonomous vehicle (AV)). The subject of this study were healthy adult females who had experience in growing crops. We measured workers' heart rate, blood Pressure. rectal temperature, mean skin temperature, oxygen consumption and blood lactate level as a physiological index of work load. The results of this study are as follows : The test group using experimental vehicle showed the lower heart rate (mean$\pm$S.D. for HV, AV respectively 74$\pm$5, 75$\pm$3 beats/min, p<0.01) than the control group (84$\pm$8beat/min) not using experimental vehicle and the lower systolic blood Pressure (HV, AV respectively 109$\pm$8, 109$\pm$9 mmHg, p<0.01) than the control group (121$\pm$11 mmHg), and lower rectal temperature(HV, AV respectively 37.0$\pm$0.1, 36.8$\pm$0.2$^{\circ}C$, p<0.01) than the control group (37.0$\pm$0.2$^{\circ}C$), and the less oxygen consumption (HV, AV respectively 2.13$\pm$0.09, 1.66$\pm$0.52$m\ell$/kg/min, p<0.01) than the control group(2.43$\pm$0.12$m\ell$/kg/min), and the lower blood lactate level (HV, AV respectively 2.03$\pm$1.00, 1.66$\pm$0.52mmol, p<0.01) than the control group (2.43$\pm$0.12mmol). Judging from these results, these experimental vehicles for greenhouse workers can be confirmed as a useful tool. It is suggested that these vehicles would alleviate the peasant's syndrome including muscle fatigue and musculoskeletal disease usually caused by working in an uncomfortable posture.

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