• Korean journal of applied entomology
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• v.53 no.4
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• pp.355-365
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• 2014

It has been unclear whether the diamondback moth, Plutella xylostella can overwinter in Korean field conditions. This study determined overwintering conditions of P. xylostella by conducting field exposure tests based on its cold tolerance and monitoring overwintering populations by direct examination of overwintering larval habitats and capturing adults with sex pheromone traps. In addition, the overwintering populations were analyzed using polymorphic genetic markers to trace their sources. When all immature stages of P. xylostella were exposed to $-5^{\circ}C$, which was the temperature much above their supercooling points, they significantly suffered with direct cold injuries, where larval stage was most tolerant to the cold injury. However, the exposure to $5^{\circ}C$ for a long period (4 weeks) did not give any significant cold injury to nonfeeding stages, while this treatment gave lethality to larval stage without diet. When all developmental stages of P. xylostella were exposed to open field conditions during winter, they exhibited significant decreases of survival rates. However, some protected and indoor conditions reduced the cold injuries and the diet provision significantly increased larval survival rates. Adult monitoring with sex pheromone during winter period indicated that the first captures were observed at similar periods at different locations (${\approx}260$ Km apart). The overwintering adults were captured until early April. Genetic variation of these overwintering populations was analyzed with polymorphic molecular markers, indicating significant genetic divergences among the overwintering populations. This study indicates that P. xylostella can overwinter in southern Korean fields or some protected greenhouses with host plants.

• Korean journal of applied entomology
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• v.59 no.3
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• pp.185-202
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• 2020

The diapause induction season in Ostrinia furnacalis (Lepidoptera: Crambidae) was estimated in Suwon. Three batches of adult generations were observed, the first one from early May to early July, the second from early or mid-July to early or mid-August, and the third from mid-August to October. In outdoor larval rearing, colony rearing occurring from mid-July to mid-August produced both non-overwintering and overwintering larvae, whereas late-reared colonies produced only overwintering larvae. Larvae collected during July and August in maize fields produced both non-overwintering and overwintering larvae, whereas late-collected larvae produced only overwintering larvae. The results indicated that O. furnacalis has a bi- or trivoltine complex life cycle in this area. In the laboratory, when larvae of all instars within 9 h after molting were first treated to a diapause induction condition (11:13 h = light:dark photoperiod and 20℃), almost all larvae were induced to diapause. However, when similar treatments were conducted age-specifically for the 5th instar larvae, diapause induction rates in 3- and 4-day-old larvae of the 5th instar decreased. In contrast, when larvae were subjected to the diapause induction treatment only during the periods from the hatching stage to the 2nd, 3rd, and 4th instar, almost all larvae were not induced to diapause. The results suggest that the early age of the 5th larval instar is the last stage for sensitivity to diapause induction stimuli. In the diapause-induced larvae, hemolymph trehalose content increased and body supercooling points dropped, compared with those in non-diapause larvae.

• Korean journal of applied entomology
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• v.62 no.4
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• pp.227-243
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• 2023

The Asiatic pink stem borer, Sesamia inferens (Walker, 1856) (Lepidoptera: Noctuidae) is a pest that attacks rice stems. In this study, we estimated the annual generation of insect in several regions of the Republic of Korea. Adult trapping using a sex pheromone trap detected the occurrence of S. inferens adults in the three northernmost areas around 38° latitude and showed that the insect inhabits all of Korea. In most areas investigated, the seasons of the adult generations estimated using the single-sine degree-day model did not deviate from the corresponding observed seasons of adult occurrence. We estimated that the overwintering larvae hypothetically-estimated using the model could be originated from the last generation of adults. When larvae collected in paddy fields during the autumn season in a few middle and southern areas were reared at 25℃, ca. 70% of pupae did not show additional larval molting before their pupation. All larvae collected in early March in a southern area (Goseong, Gyeongsangnam-do) pupated without additional larval molting when reared at 25℃. Based on these results, we presumed that S. inferens could overwinter as mainly the last instar larval stage in the area. Taken together, we conclude that S. inferens primarily has two generations per year in areas around 38° latitude, and three generations in the areas between 35.3° and 37.3° latitude. In addition, approximately 35% of insects captured by the sex pheromone trap were species other than S. inferens, as determined by analyzing the nucleotide sequences of the cytochrome c oxidase 1 gene. These species were not morphologically misidentified as S. inferens.

• Korean journal of applied entomology
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• v.43 no.3 s.136
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• pp.201-209
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• 2004

Overwintering ecology of the peach pyralid moth, Dichocrosis punctiferalis Guenee (Lepidoptera: Pyralidae) was studied in laboratory and in a chestnut orchard in 2001/2002 winter and 2003 spring. All twenty-four female and fourteen male moths were fruit-feeding type which were emerged from the larvae collected at the same chestnut orchard in 2003 winter. Periodical thermal treatment of overwintering larvae at $25^{\circ}C$ revealed that diapause termination of the larval population started from the end of January and was completed until April 20 in southern region of Korea. Pupation of diapause-terminated larvae started from early May and reached to $100\%$ at June 3 in chestnut orchard. Days to pupation of female larvae after diapause termination were 12.5, 8.9, and 7.5 at $20^{\circ}C,\;25^{\circ}C$, and $30^{\circ}C$, respectively. Those of male larvae were similar to those of females. Pupal periods of female were 14.7, 11.8, and 9.0 days at the three temperatures. Moths of the overwintered generation emerged from May 20 to June 28, and reached to $50\%$ emergence on June 8 to 9 under emergence cage. Attraction of the male moths of overwintered generation to sex pheromone traps showed that the date of $50\%$ catches to the traps were June 17, when data from 3 southern regions of Korea were pooled.

• Journal of Environmental Science International
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• v.26 no.1
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• pp.47-54
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• 2017

We analyzed the variation in occurrence time and intensity of attacks by the rice stem borer (Chilo suppressalis Walker) on several crops. This study aimed to understand how C. suppressalis damages crops and to utilize this basic data for the establishment of environment-friendly control methods like pheromone traps. This study surveyed the changes in occurrence patterns of C. suppressalis, biological characteristics of overwintering larvae, and the efficacy of different types of pheromone traps the years. We found similar occurrence patterns of C. suppressalis in different crops. In addition, occurrence time of the first generation was advanced. Overwintering larvae showed no difference in pupal period and weight compared to the non-overwintering ones. However, the larval period was reduced to 19.1 days in Miscanthus field, in contrast to that in the paddy fields. It was confirmed that larvae of C. suppressalis generally prefer the lower part of the stems of Miscanthus. Efficiency of the emergence trap was confirmed to be greater than that of the delta trap for capturing C. suppressalis adults. However, it is necessary to adjust the control period because of the advancement in occurring time of C. suppressalis in recent years. The larvae of C. suppressalis experience favorable environmental conditions for overwintering in Miscanthus fields. The major Miscanthus fields are generally located in the areas protected for sources of drinking water, owing to which spraying of chemical pesticides is very limited. The results of this study provide important inputs for the development of environment-friendly control methods.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.295-299
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• 1995

This study was conducted to find the control methods, status of overwintering and seasonal occurrence of Erioccoccus largerstroemiae on persimmon trees in Ch'ondo area, Kyongbuk province in Korea. Usually, overwintering eggs were sheltered in bark, among them the rate of alive eggs was 28.7%. The activiation of garpe-myrtle scale showed from late April, and they have three generations per year. The first occurrence of larval stage of Erioccoccus largerstroemiae starts from late June to early July, the second occurrence begins from middle August to late August, and from late June to early July, the second occurrence begins from middle August to late August, and from middle September to late September is the third, thus, three peaks of occurrence revealed in early July middle August, and late September respectively. Average number of eggs conceived in female adult was 229.3. Spray effect of chemical control showed as follows; lime sulfur with tow applications of pesticides (late June, late August) at 97.8%, machine oil with tow applications of pesticides (late June, late August) at 96.8%. And during the growing period, the spray results using three applications of pesticide only (late June, late August, amid September) showed 77.2% in field condition.

• Korean journal of applied entomology
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• v.30 no.4
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• pp.258-264
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• 1991

Studies were carried out to investigate effects of temperature and photoperiod on diapause induction and of juvenile hormone III and 20-hydroxyecdysone treatment on diapausing adults of the alder leaf beetle, Agelastica coerulea Baly(Chrγsomelidae: Coleoptera). Its life cycle and ovarian development in adults were also observed. The beetle had one year life cycle with egg, larval, pupal and adult periods being 7-10, 19-21, 14-15 days and about 10 months, r respectively. All adults showed a diapause syndrome when the larvae were reared at $20^{\circ}C$ or $25^{\circ}C$ in combination of photoperiods of 16L/8D, 12L/12D, or 8L/16D. Their ovarioles did not s show any development of vitellogenesis before or during diapause and even when exposed at $15^{\circ}C$ after overwintering. When diapausing adults were treated with JH III they resumed feeding and laid several eggs and broke diapause condition temporally. But diapausing adults treated with 20-hydroxyecdysone did not show any response.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.257-268
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• 1990

The regional characteristics in the occurrence of the rice stem borer, Chilo suppressalis (Walker), in Korea, were examined by analyzing the light trap data obtained from 39 locations during the years of 1965-1988, and some ecological significance of the overwintering larval populations collected from the four locations where some of the typical ecological-race have been known, the results obtained are as follows; The total number of the moths have decreased greatly throughout the nation since 1970. The decrease in the total number of moths related with those in the spring moth but not in the summer moths, and suggested that the changes in the rice production systems have effected significantly on the second generation of the insect. The variations between the regions had been gret in 1960's, decreasing phase in 1970's and increasing phase in loast decade. The overall average of 50% emergence dates have shifted earlier in both moths, and the changes were different considerably with the regions as wheel as in generations. When the overwintered larvae were incubated at $25^{\circ}C$ and RH of 90%, the average mortalities were 21.07% for Suweon, 43.06% for Iri, 63.51% for Jinju, 27.04% for Gwangju. When the overwintered larvae were incubated at $25^{\circ}C$ and RH of 90%, the average mortalities were different with the body weight; 45.44% for the larvae of less than 39 mg, 37.61% for 40-49 mg, 37.28% for 50-59 mg, 30.17% for greater than 60 mg. When the overwintered larvae wee incubated at $25^{\circ}C$, post-diapause larval development periods of body weight classes were 18.6$\pm$1.2 days for the larvae of less than 39 mg, 20.4$\pm$3.3 days for 40-49 mg, 21.4$\pm$3.2 days for 50-59 mg, 21.8$\pm$3.2 days for grater than 60 mg, and only the larvae of less than 39 mg, body weight class less than 39 mg was found statistically different in post-diapause larval development periods. From these results, the changes in the cultural practice of the rice have effected on the ecological pattern of the rice stem borer, and the effects seemed to be different with the generations, and with the regions where the different ecological races are distributing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.369-374
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• 2014

The rice stem borer (Chilo suppressalis Walker) was one of the most destructive pest of rice for the 1960s and 1970s in Korea. Recently, it is newly recognized as a potential risk factor to the biomass yield of bioenergy crops. The current research was firstly conducted to investigate overwintering larvae population density and pattern of rice stem borer attacking Miscanthus sacchariflorus cv. Geodae 1 which is referred to as an ideal lignocellulosic bioenergy crop in Korea. Population density of larvae per $1m^2$ in stems and rhizomes at the Miscanthus experimental plots and rates of damage (wormhole, abscission) of M. sacchariflorus cv. Goedae 1 were investigated from October 2012 to March 2013. The population of larvae per $1m^2$ in stems of Miscanthus were 23, 4, 1, and 1 in October, November, December 2012, and January 2013, respectively. Over the same period, the population of larvae in basal stem rots and rhizomes were increased, whereas decreased in stems. Interestingly, the positions of larvae for overwintering in Miscanthus were confirmed to 5~10 cm below the soil surface such as basal stem rot and rhizome, whereas the most common overwintering position known in rice is a part of stem on the ground such as rice straw and rice stubble. It would suggest that the larvae gradually moved to bottom of stems and rhizomes in soil in line with decline in temperature. Moreover, the damage rates of stems per $1m^2$ were up to more than 50% in some places. In conclusion, this might be the first report that rice stem borer could affect the productivity of biomass of Miscanthus in case of mass cultivation. Moreover, it should be necessary to make a decision in insect control management for this bioenergy feedstock and other related crops.

• Korean journal of applied entomology
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• v.30 no.4
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• pp.249-257
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• 1991

Chronological changes in the occurrence pattern of the striped rice borer(SRB), Chilo suppressalis Walker, were investigated by analyzing the data collected by 33 light traps of the countrywide monitoring units during the period of 1968-1989. And also relation of incidences between larval and adult populations, emergence time and percent emergence of overwintered larvae were observed in relation to the time of different rice transplantations. Spring moths of SRB in 1985-'89 generally emerged earlier than in 1968-'72 : 30 days earlier at Kwangju, 20 -25 days earlier at Sangju and Naju, 10-15 days earlier at Uljin, Jinju, Goyang, Milyang, Kimhae, etc., 5 days earlier at Suweon, Jinchun, Daejeon, Namweon, Haenam, etc. There was no change in the time of spring moth emergance at Weonseong, Chungju, Seosan, Yongju, and Nonsan. Summer moths of SRB in 1985- '89 also emerged earlier than 1968- '72: 20 days earlier at Uljin, 15 days earlier at Chungju, Yeongdug, and Habchun, 5-10 days earlier at the other areas except Yeongju. There was an overall countrywide decrease in the population of the 1st generation of SRB during the last two decades. The population of the 2nd generation also decreased in 19 areas during the same period, but increased in 14 areas including over 3-fold increases in Kwangju, Naju and Daegu, and double increase in Jinju, Milyang and Gongju. The higher larval population density of 1st genration was taken place in the earlier transplanted fields, whereas the population of 2nd generation were higher at mid-season transplanted (May 30), followed by May 15, June 15, and April 30. The peak of larval population appeared generally 15 days after moth peak in 1st generation, and 10 days after moth-peak in 2nd generation. The earlier transplanted fields were the higher percent damage due to 1st off-springs of spring moths, whereas the fields transplanted in mid-season were higher damage due to 2nd off-springs of summer moths. Average body weights of SRB larvae before overwintering were 65.6 mg, 61.2 mg, and 55.5 mg in early, mid-season, and late transplantations, respectively. In field cage experiments, emergence rate of the overwintered larvae ranged from 28.3-39.8%. In other words, body weights of overwintering larvae were heavier in fields by earlier transplantation, and heavier larvae showed higher percentage of adult emergence. The period from overwintered larvae to adult emergence was longer in the fields of the later transplantations; namely, 44, 49, and 51 days for early, mid-season, and late transplantations, respectively.