• Animal Bioscience
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• v.36 no.12
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• pp.1889-1897
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• 2023

Objective: 'Cultured meat' has been suggested as means of solving the problems associated with overpopulation and gas emissions. Satellite cells are a major component in the production of cultured meat; however, these cells cannot be maintained in vitro over long periods. Fibronectin is a glycoprotein that affects biological processes such as cell adhesion, differentiation, and migration. Unfortunately, the characteristics of porcine satellite cells grown in a long-term culture when exposed to fibronectin-coated dishes are unknown. The objective of this study was to investigate the appropriate concentration of fibronectin coated dishes for proliferation and maintenance of porcine satellite cells at long-term culture. Methods: In this study, we isolated the satellite cells and fibroblast cells with pre-plating method. We next analyzed the cell doubling time, cell cycle, and rate of expressed paired box 7 (Pax7) and myogenic differentiation 1 (MyoD1) in porcine satellite cells cultured with 20 ㎍/mL of fibronectin-, gelatin-, and non-coated dishes at early and late passage. We then analyzed the proliferation of porcine satellite cells with various concentrations of mixed gelatin/fibronectin. We next determined the optimal concentration of fibronectin that would encourage proliferation and maintenance of porcine satellite cells in a long-term culture. Results: Doubling time was lowest when 20 ㎍/mL of fibronectin was used (as tested during an early and late passage). Levels of expressed Pax7 and MyoD1, assessed using immunocytochemistry, were highest in cells grown using fibronectin-coated dishes. The proliferation of gelatin/fibronectin mixed coatings had no significant effect on porcine satellite cells. The concentration of 5 ㎍/mL fibronectin coated dishes showed the lowest doubling time and maintained expression of Pax7. Conclusion: Fibronectin with 5㎍/mL effectively maintains porcine satellite cells, a discovery that will be of interest to those developing the next generation of artificial meats.

• Tunnel and Underground Space
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• v.19 no.3
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• pp.236-247
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• 2009

Overpopulation has significantly increased the use of underground spaces in urban areas, and led to the developments of shallow-depth underground space. Due to unexpected rock fall, however, it is very necessary to understand and categorize the rock mass behaviors prior to the tunnel excavation, by which unnecessary casualties and economic loss could be prevented. In case of cave-in, special attention should be drawn since it occurs faster and greater in magnitude compared to rock fall and plastic deformation. Types of cave-in behavior are explained and categorized using seven parameters - Uniaxial Compressive Strength (UCS), Rock Quality Designation (RQD), joint surface condition, in-situ stress condition, ground water condition, earthquake & ground vibration, tunnel span. This study eventually introduces a new index called Cave-in Behavior Index (CBI) which explains the behavior of cave-in under given in-situ conditions expressed by the seven parameters. In order to assess the mutual interactions of the seven parameters and to evaluate the weighting factors for all the interactions, survey data of the experts' opinions and Rock Engineering Systems (RES) were used due to lack of field observations. CBI was applied to the tunnel site of Seoul Metro Line No. 9. UDEC analyses on 288 cases were done and occurrences of cave-in in every simulation were examined. Analyses on the results of 288 cases of simulations revealed that the average CBI for the cases when cave-in for different patterns of tunnel support was estimated by a logistic regression analysis.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.4
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• pp.39-53
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• 2013

Although the efficient formation of urban space structure is a key factor in energy saving and environmentally-friendly aspect, the maintenance of the center and sub-center of the city that are key factors has been becoming increasingly difficult due to the variability and complexity of urban activities. In the case of Busan, amid the expansion of urban scale due to rapid economic development and overpopulation, systematic approaches to professional diagnosis and maintenance have been significantly insufficient - other than the city basic plan which has been conducted at an interval of 20 years. For the effective management of urban central area, systematic monitoring of the CBD through demand forecast and blight forecast at a city level must first be implemented. In order to fulfill this goal, this study is to figure out the current state of the CBD through the diagnosis on blight of the urban central area in the viewpoint of rehabilitation of the CBD and to propose the measures for practical utilization of the information about space for the further management of the central area of the city. For analysis, the study looks into the present state in terms of physical index, economic index, and social index. And then as a micro-approach by utilizing economic index, the study has thoroughly examined the economic blight of the Seomyun urban central area of Busan. The outcome of the analysis shows that in terms of population distribution and land utilization the area is in the stage of inefficient dispersion after having gone through the stage of suburbanization. It is expected that this study, as the material that proves the necessity of enhancing the function of the CBD, can propose the direction for the management of the urban center of Busan through blight prediction and management of the urban center and can provide the basic data for the long-term urban development that aims at the efficient strengthening of functions of the CBD.

• Fisheries and Aquatic Sciences
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• v.22 no.9
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• pp.20.1-20.8
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• 2019

Background: Despite the favorable geo-climatic potential of Cameroon, the national production of tilapia remains low due to poor tilapia growth reported by fish farmers. One of the underlying reasons is the early female maturation at a very small size and precocious breeding in earthen ponds, resulting in overpopulation which leads to stunted growth and therefore to the production of unmarketable fish size. Studies have shown that dietary supplementation of G. kola enhanced growth in young Clarias gariepinus and Oreochromis niloticus. It was also reported that G. kola inhibited spawning in Tilapia adult females. Therefore, this study sought to assess the effects of Garcinia kola as growth promoter and inhibitor of gonadal development in young Oreochromis niloticus. Methods: A total of 108 juveniles weighing $13.32{\pm}0.62g$ were randomly distributed in 9 hapas of 12 fishes each (9 females and 3 males) and fed for 70 days with three isonitrogenous diets, 40% crude protein with increasing Garcinia kola supplementation levels of 0 (normal diet), 6% and 10% (experimental diets). Physico-chemical parameters of the water (temperature, dissolved oxygen, pH, nitrate, nitrite, ammonia, and transparency) were measured twice a week. Every 14 days, fish were harvested, counted, and weighed. At the end of the experiment, three fish of each sex per replicate were sacrificed and their gonad and liver collected and weighed. Data were statistically analyzed using one-way analysis of variance repeated measure followed by Newman-Keuls multiple tests. Results: The results showed that all physico-chemical parameters of the water were within the recommended values for Tilapia culture. Tilapia fed 6% Garcinia kola supplemented diet displayed higher final body weight in males ($38.60{\pm}3.50g$) and females ($36.77{\pm}3.62g$) compared to those receiving normal diet ($36.23{\pm}1.36g$ and $25.87{\pm}3.32g$; respectively to the final body weight in males and females). The gonadosomatic index and hepatosomatic index indicated no significant variation in males while in females, these were significantly low in the experimental fish compared to control fish. Conclusion: The results of this study demonstrated that supplementation of G. kola seeds in diets of young Tilapia improved growth performance and impaired gonadal development in females.

• Journal of Korean Tunnelling and Underground Space Association
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• v.19 no.3
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• pp.421-435
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• 2017

Recently, due to the saturation of ground structures and the overpopulation of pipeline facilities requires to development of underground structures as an alternative to ground structures. Thus, mechanized tunnel construction of the shield TBM method has been increasing in order to prevent vibration and noise problems in construction of the NATM tunnel for the urban infrastructure construction. Tunnel construction plan for the tunnel line should be formed in a sharp curve to avoid building foundation and underground structures and it is inevitable to develop a shield TBM technology that suits the sharp curve tunnel construction. Therefore, this study is about the structural stability technology of the articulation jack, shield jack and skin plate for the shield TBM thrust in case of the mechanized tunnel construction that is a straight and sharp curve line. The construction case study and shield TBM operation principle are examined and analyzed by the theoretical approach. The torque of the cutter head, the thrust of the articulation jack and the shield jack, the amount of over cutting for curve is important respectively in shield TBM construction of straight and sharp curve line. In addition, it is very important to secure the stability of the skin plate structure to ensure the safety of the inside worker. This study examines the general structure and construction of the equipment, experimental simulation was carried out through numerical analysis to examine the main factors and structural stability of the skin plate structure. The structural stability of the skin plate was evaluated and optimizes the shape by comparing the loads of the articulation jack by selecting the virtual soil to be applied in a straight and sharp curve line construction. Since the present structure and operation method of the shield TBM type in domestic constructions are very similar, this study will help to develop the localized shield TBM technology for the new equipment and the vulnerability and stability review.

• Korea journal of population studies
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• v.27 no.1
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• pp.171-201
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• 2004

Modern sciences in the West are deeply rooted in the Greek and Roman cultural heritage. Consequently, the academic achievements accomplished by the scholars of the Arabs including Persian world, the profound thoughts developed in the Indian subcontinent, and the excellent works made by the East Asian scholars have mostly been neglected in the past. This paper attempts to compare the thoughts and theories on population developed by the Western scholars with those of East Asian scolars, in chronological order, ancient, mediaeval, and modern period before Malthus. The thesis that excessive population growth may reduce output per worker, depress levels of living for the masses and engender strife is of great antiquity. In fact, overpopulation in East Asia, especially in China, goes back to very ancient times, most Confucian scholars maintained the notion of a numerical balance between population and environment. They also looked for means to check the increase in numbers. The foundations of a theory of optimum population level, fully developed in the twentieth century, can be found in their writings. Although early population thoughts in China had not advanced far in the analysis of the significance of population size and growth, it had taken only a few steps forward. At some times and to some observers, populousness appeared desirable; at other times and to other observers, it seemed all too evident that the number of people could become too great. These viewpoints foreshowed some of the later developments. The early population literatures reviewed here seem to consist of a number of quite isolated contributions. In fact, however, there may have been a greater continuity of thought than now appears, for many of the contributions may have been lost and there are many gaps in the record. An intensive review on comparing two works, those of Malthus' and Hong, Liang-chi's, are presented in this paper. Only five years before Malthus published his famous work, An Essay on the Principle of Population, Hong published his theories on population. Some of them, Hong insisted, are very similar to the Malthusian concepts of geometrical increase, natural and artificial checks of population. Despite the excellent works in the ancient period, this paper concludes with an investigation of the reasons why modem achievements in scientific areas in the East Asia have been far behind that of the West.