• Korean journal of applied entomology
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• v.34 no.3
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• pp.181-190
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• 1995

Malate dehydrogenase in the mosquito ovary after a blood meal, Aedes aegypti, was purified and characterized. MDH purification steps involved DEAE-Sepharose, S-Sepharose and Cibacron blue affinity chromatography. The purified MDH was 70,000 daltons in molecular weight and was a homodimer consisting of tow identical subunits. Optimal activity of purified MDH was obtained pH 9.0-9.2 in malate-oxaloacetate reaction and pH 9.8-10.2, in oxaloactate-malate reaction. With obtained pH 9.0-92 in malate-oxaloacetate reaction and pH 9.8-10.2, in oxaloactate-malate reaction. With malate as substrate, purified mitochondrial MDH (1.28$\times$${10}^{-4}$ M) had lower Km value than cytoplasmic MDH (8.92x${10}^{-3}$ M). MDH activity was inhibited by citrate, $\alpha$-ketoglutarate, and ATP. Inhibition of MDH activity by ATP and citrate was less in malate-oxaloacetate reaction and in oxaloacetate-malate reaction. MDH activity was completely inhibited by ATP in oxaloacetate-malate reaction and not inhibited by citrate in malate-oxaloacetate reaction. Temporal activity change of MDH is similar to that of isocitrate dehydrogenase in the ovary after blood feeding; their activities in the ovary began to rise at 18 hours after a blood meal, and reached at the maximal level at 48 hours.

• The Journal of Korean Obstetrics and Gynecology
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• v.32 no.1
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• pp.37-47
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• 2019

Objectives: The purpose of this study was to analyze the current characteristics and prescriptions of outpatients with Polycystic Ovary Syndrome (PCOS). Methods: We searched medical records from January 1, 2017 to December 31, 2017 and found out 31 patients who first visited with PCOS. Results: The average age of PCOS patients was $26.48{\pm}5.15years$ old, average height was $162.16{\pm}5.56cm$, average weight was $55.27{\pm}9.34kg$, and average BMI was $21.01{\pm}3.48kg/m^2$ A total of 24 patients who had received western treatment for PCOS in the past, 13 people received oral contraceptions, 4 hormones, 2 metformin, one hormone and metformin, also 4 people were treated for assisted reproductive technologies due to infertility. There were only two patients who combined Korean and Western treatments. The most common menstrual-related symptoms of PCOS patients were oligomenorrhea (48.4%) and dysmenorrhea (22.6%). Other symptoms were the highest in the cold symptoms (54.8%), followed by infertility (9.7%). The average number of patients visiting the hospital was $6.26{\pm}7.2$, with 22 (71.0%) coming from 1 to 5. The average treatment period for patients was $10.1{\pm}10.8weeks$, with 41.9% the largest for 1 to 4 weeks. Acupuncture (93.5%), moxibustion (96.8%) herbmed (96.8%) was treated to most PCOS patients. The most commonly used herbal medicines were Ongyeong-tang (35.5%), Ijin-tang-gami (19.4%), Dodam-tang-gami (12.9%), and Jogyeongjongok-tang-gami (12.9%). Conclusions: These results could be helpful to treat PCOS patients in Korean gynecologic clinical fields.

• Journal of the Korea Convergence Society
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• v.13 no.3
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• pp.337-343
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• 2022

The aim of this study was to evaluate anxiety, depression and stress in women with polycystic ovary syndrome(PCOS) and to investigate the risk factors related to psychological difficulties. Sixty women with PCOS were evaluated for level of psychological stress using Beck depression inventory(BDI) and Depression anxiety stress scale(DASS) questionnaire. Serum antimullerian hormone, total testosterone, lutenizing hormone, follicle stimulating hormone, estradiol, lipid profile and 75g oral glucose tolerance test were measured. Thirty healthy women served as the control. Fifty two women with PCOS and 29 healthy women completed a questionnaire. Women with depression who scored >13 by BDI and >10 by DASS were 38.5 %, women with anxiety who scored >8 by DASS were 23.1 %, and women with stress who scored >15 by DASS were 30.8 %, which were significantly higher than control. In PCOS women, total testosterone, LH and AMH were significantly correlated with depression and stress. Weight, body mass index and waist-hip ratio were also significantly correlated with depression. In women diagnosed as diabetes and hyperlipidemia, depression and stress were significantly prevalent. Women with PCOS seemed to be more vulnerable to depression, anxiety and stress. Early diagnosis and management should be considered.

• Toxicological Research
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• v.26 no.3
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• pp.209-216
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• 2010

Limb bud (LB) and central nerve system (CNS) cells were prepared from 12.5 day old pregnant female Crj:CD (SD) rats and treated with olaquindox and vitamin A. Cytotoxicity and inhibition on differentiation were measured in each cell. Three doses of olaquindox (4, 21 and 100 mgkg), and 0.2 and 75 mg/kg of vitamin A were administered to pregnant rat for 11 days from $6^{th}$ to $16^{th}$ of pregnancy. $IC_{50}$ values of olaquindox for proliferation and differentiation in CNS cell were 22.74 and $28.32\;{\mu}g/ml$ and 79.34 and $23.29\;{\mu}g/ml$ in LB cell and those values of vitamin A were 8.13 and $5.94\;{\mu}g/ml$ in CNS cell and 0.81 and $0.05\;{\mu}g/ml$ in LB cell, respectively. Mean body weights of pregnant rats were decreased at high dose of olaquindox (110 mg/kg) but relative ovary weight, number of corpus lutea, and number of implantation were not changed. Resorption and dead fetus were increased at high dose of olaquindox, and relative ovary weight, the number of corpus lutea and implantation, and sex ratio of male to female were not significantly changed in all dose of olaquindox. Mean fetal and placenta weights were significantly (p < 0.01) decreased in rats of high group. Seven fetuses out of 103 showed external anomaly like bent tail, and 10 out of 114 fetuses showed visceral anomalies at high group. The ossification of sternebrae and metacarpals were significantly (p < 0.01) increased by low and middle dose of olaquindox but it was significantly (p < 0.01) prohibited by high dose of olaquindox. In rats treated with vitamin A, the resorption and dead fetus were increased by high dose. Mean fetal weights were significantly (p < 0.01) increased by low dose but significantly (p < 0.01) decreased by high dose. Thirty four fetuses out of 52 showed external anomaly; bent tail (1), cranioarchschisis (14), exencephaly (14), dome shaped head (22), anophthalmia (15), brcahynathia (10) and others (19). Forty five fetuses out of 52 showed soft tissue anomaly; cleft palate (42/52) and anophthalmia (22/52) by high dose of vitamin A. Sixty one fetuses out of 61 (85.2%) showed skull anomaly; defect of frontal, partial and occipital bone (21/61), defect of palatine bone (52/61) and others (50/61). In summary, we support that vitamin A is strong teratogen based on our micromass and in vivo data, and olaquindox has a weak teratogenic potential in LB cell but not in CNS cell. We provide the in vivo evidence that a high dose of olaquindox could have weak embryotoxic potential in rats.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.3
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• pp.48-72
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• 2011

Objectives: This study was designed to investigate the effects of Yongdamsagantang on the polycystic ovary(PCO) induced by estradiol valerate(EV) in rats. Methods: After administrating Yongdamsagan-tang to PCO induced rats, we measured the weight of body, ovaries, adrenal glands, and uterus of rats. The observation through naked eye and histopathological observation of ovaries were evaluated. Also, the number of follicle and corpora lutea and content of androstenedione(ADD) and total estrogen were evaluated. The expressions of nerve growth factor(NGF) and corticotropin releasing factor(CRF) were analyzed by immunohistochemistry. The breeding rate and number of implantation with normal male rats were evaluated. Results: - The weight(mg) of ovaries in YST treated group($73.8{\pm}7.6$) was significantly increased(p<0.001) compared with control group($54.3{\pm}4.5$). - The number of mature follicles in YST treated group($7.3{\pm}2.4$) was significantly increased(p<0.01) compared with control group($3.5{\pm}1.2$). - The number of atretic follicles in YST treated group($9.0{\pm}1.5$) was significantly decreased(p<0.01) compared with control group($13.4{\pm}3.8$). - The number of cystic follicles in YST treated group($3.1{\pm}1.1$) was significantly decreased(p<0.01) compared with control group($6.0{\pm}2.0$). - The number of corpora lutea in YST treated group($3.8{\pm}2.1$) was significantly increased(p<0.001) compared with control group($0.3{\pm}0.7$). - The expression of NGF-immunoreactive cells in the ovarian granulosa cells in YST treated group was lesser observed than control group. - The expression of NGF-immunoreactive cells in the adrenal cortex in YST treated group was lesser observed than control group. - The breeding rate in YST treated group(100 %) was significantly increased (p<0.05) compared with control group(50 %). - The number of implantation in YST treated group($6.4{\pm}4.7$) was significantly increased(p<0.05) compared with control group($1.4{\pm}2.6$). Conclusions: We concluded that Yongdamsagan-tang activates the maturation of follicles, normal ovulation, breeding rate and number of implantation. Therefore, this may be effective for the treatment of anovulation, amenorrhea and sterility of PCOS patients.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.4
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• pp.236-246
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• 2023

Background: This study helps to evaluate the Ovarian potential of Cameroonian Zebu cattle (Bos indicus) slaughtered in Etoudi - Yaoundé for implementing Assisted Reproductive Techniques (ARTs). The aim was to enhance the productivity of the cattle sub-sector in Cameroon while conserving local genetic resources. Methods: A total of 144 cows, including 94 cycled cows and 50 pregnant cows, were included in the study. Live weights were determined by measuring the thoracic perimeter of each cow using a Rondo measuring tape. Age was determined postmortem through examination of dentition and horns, while the uterus and ovaries were removed, weighed, and classified based on physiological status (pregnant or nonpregnant). Follicles were counted, and their diameters were measured and categorized into small (Ø < 3 mm), medium (Ø 3-8 mm), and large (Ø > 8 mm). Results: The results revealed an average follicular population of 32.02 ± 9.31 per cow, with 22.43 ± 8.45 small follicles, 8.42 ± 3.87 medium follicles, and 0.76 ± 0.34 large follicles. The weight of the right ovary was significantly higher than that of the left ovary (p < 0.05), and cows aged 6 to 9 years exhibited a higher number of follicles compared to other age groups. Cows with medium (BCS = 3) and large (BCS = 4-5) body reserves had the heaviest ovaries. Additionally, pregnant cows had heavier uteri compared to non-pregnant cows, and cows with a body condition score of 3 or higher had higher uterine weights than lean cows (BCS = 1-2). Conclusions: This study demonstrates that age, body condition score, and pregnancy status influence ovarian weight. Body Condition Score serves as a reliable indicator of cow nutritional status, and cows with BCS of 3-5 are excellent candidates for in vitro production of embryos.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.4
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• pp.253-260
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• 2008

Gonadal development, gametogenesis, reproductive cycle, spawning, relative weight of flesh, and onset of sexual maturity of the murex shell, Ceratostoma rorifluum, collected from the rocky intertidal zone of Daehang-ri, Buan-gun, Jeollabuk-do, Korea were investigated monthly from January to December 2005 both cytologically and histologically. The gonads were widely placed on the digestive gland located in the posterior spiral fleshy part in the shell. C. rorifluum had separate sexes, and was an internal fertilizer. The sex ratio of females to males was approximately 1:1. The ovary and testis contained a great number of oogenic follicles and spermatogenic tubules, respectively. The oogonia and fully ripe oocytes were $15-19{\mu}m$ and $150-160{\mu}m$ in diameter, respectively, and the cytoplasm of the ripe oocytes contained a number of yolk granules. The relative weight of flesh reached a maximum in August($39.35{\pm}0.40%$), and then decreased rapidly in November($32.75{\pm}1.20%$). The percentages of female and male snails at first sexual maturity with shell heights ranging from 12.1-14.0 mm were 60.0% and 52.9%, respectively, while 100% of the snails of both sexes with shell heights over 18.1 mm were reproductively active. Based on the gonadal development and histological observations, the reproductive cycle of the snail could be categorized into five successive stages: early active(December to May), late active(March to July), ripe(June to September), spawning(July to October), and recovery(October to March). C. rorifluum spawned once a year between July and October, and the majority of spawning occurred in September when the seawater temperature exceeded $23.5^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.5
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• pp.385-392
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• 2004

Gonadal development, gametogenesis, reproductive cycle, gonad index, and flesh weight rate of the murex shell (Ocenebra japonica) collected from the rocky intertidal zone of Buan-gun, Jeollabuk-do, Korea were investigated by means of histological method from January to December 2002. O. japonica had separate sexes, and was oviparous. The gonad was widely situated on the surface of the digestive gland located in the rear of the spiral flesh part in the shell. The male penis was located near the two tentacles. The ovary was composed of a number of oogenic follicles, and the testis was composed of several spermatogenic tubules. The size of ripe oocyte was approximately $140{\mu}m$ in diameter. The gonad index (GI) began to increase in March $(33.24{\pm}2.33)$ and reached the maximum in June $(47.77{\pm}1.90)$ Thereafter, the values decreased from July $(45.12{\pm}3.60)$ to October $(19.32{\pm}2.91)$. The flesh weight rate (FWR) began to increase in January $(25.93{\pm}1.32)$ and reached the maxium in May $(31.78{\pm}1.09)$ Thereafter, the values decreased from June $(31.50{\pm}0.66)$ to October $(24.09{\pm}1.60)$. The reproductive cycle could be classified into five successive stages: early active (October to April), late active (January to June), ripe (May to September), spawning (July to September) and recovery (September to February). The reproductive cycle was closely related to the seawater temperature.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.9
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• pp.1361-1366
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• 2007

In this project, ovarian size and activity during the peak (November-April) and the low (May-October) breeding seasons in young and adult camels were studied. Ovaries of 92 camels (Camelus dromedarius), with clinically normal reproductive tracts, aged 3-15 years and slaughtered at Faisalabad or Lahore abattoirs over a period of 24 months, were collected. Jugular blood was collected from each animal before slaughter; the serum was separated and analyzed for oestradiol concentration. The size (length, width and thickness) and weight of each ovary were measured. Grossly observable Graafian follicles were counted and their diameter was measured using Vernier Calipers. The camels having ovaries presenting follicles more than 5 mm in diameter were taken as having active ovaries. The results showed that ovarian length, width and weight were significantly higher (p<0.05) during the peak than the low breeding season. The percentage of active ovaries was also significantly higher (p<0.01) during the peak than the low breeding season. However, the effect of season on ovarian thickness was non-significant. Similarly, the ovarian length, width, thickness, weight and activity did not vary significantly between young (3-7 years old) and adult (8-15 years old) animals. Serum oestradiol concentrations were significantly higher (p<0.05) during the peak ($67.70{\pm}1.36$ pg/ml) than the low breeding season ($15.25{\pm}1.54$ pg/ml). It was concluded that in Pakistani camels ovarian size and activity were higher during the peak than the low breeding season. However, age of the camel (from 3 to 15 years) had no effect on these parameters.

• The Journal of Korean Medicine
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• v.30 no.5
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• pp.102-114
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• 2009

Objectives: HMCO5 is an extract obtained from 8 different herbal mixtures. We undertook a safety evaluation of HMCO5 for a dose range finding (DRF) toxicity test in specific pathogen free (SPF) Sprague-Dawley (SD) male and female rats. Methods: The male and female rats were divided into 4 groups, respectively; G(0), treated with distilled water: G(1), treated with 222 mg/kg HMC05: G(2), treated with 667 mg/kg HMC05, and G(3), treated with 2,000 mg/kg HMC05; HMC05 was administered orally for 4 weeks. The safety evaluation examined clinical signs, mortality, body weight, food consumption, water consumption, ophthalmic findings, urinalysis, hematological values, absolute & relative organ weights, and necropsy findings during the tests. Results: There were no changes in clinical signs, mortality, body weight, food consumption, water consumption, and ophthalmic findings examined during the test periods. In serum biochemical values, triglyceride was increased in male group G(3) and Na$^+$ decreased significantly in male groups G(2), G(3) and G(4). In male group G(4), spleen weight decreased relatively and increases of absolute & relative left ovary weights were found. In addition, an adhesion of liver to diaphragm was found in male group G(2). However, we could not find any dose-interrelationships in these changes. Conclusions: These results indicate that HMC05 extract did not show any toxicity in the DRF toxicity study. Therefore, it suggests that establishment of 1,000, 333 and 111 mg/kg dosages are moderate in a repeated dose 26-week oral toxicity study of HMC05.