• Applied Microscopy
• /
• 제39권2호
• /
• pp.199-204
• /
• 2009

본 연구는 펌 염색을 한 번도 경험하지 않은 13세의 건강한 여성의 모발에 펌을 반복 시술하여 모발 표면의 형태적 변화를 주사전자현미경을 사용하여 관찰하였다. 펌1차 시술한 모발 표면은 큐티클 세포의 끝 부분들이 불규칙하게 쪼개져 나갔으며, 비늘의 가장자리 끝부분이 거칠은 상태로 관찰되었다. 그리고 큐티클세포들이 분리되고 공간이 형성되면서 표면 쪽으로 부풀어 오른 것을 확인하였다. 펌3차 시술한 모발의 경우에는 모발 표면에서 큐티클 세포와 세포 사이가 들떠서 분리되어 있고 중첩된 큐티클층 표면에 중첩된 비늘은 표면적이 불규칙하게 나타났다. 표면에 부서진 큐티클 세포의 세포질 일부가 부착되어 있으며 세포질 내의 내큐티클에는 공포가 형성되어 있었다. 펌의 횟수가 증가할수록 큐티클층과 피질의 분리가 쉽게 일어나는 것을 확인하였다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제17권6호
• /
• pp.537-546
• /
• 2013

Deiters' cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different $K^+$ currents from Deiters' cells of guinea pig using whole cell patch clamp technique. With high $K^+$ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic $K^+$ channel blocker. The remaining outward current was transient in time course whereas the clofilium-sensitive outward current showed slow inactivation and delayed rectification. Addition of 5 mM tetraethylammonium (TEA) further blocked the remaining current leaving a very fast inactivating transient outward current. Therefore, at least three different types of $K^+$ current were identified in Deiters' cells, such as fast activating and fast inactivating current, fast activating slow inactivating current, and very fast inactivating transient outward current. Physiological role of them needs to be established.

• 한방안이비인후피부과학회지
• /
• 제18권1호
• /
• pp.221-233
• /
• 2005

Background and Objectives : Despite of high prevalence, Tinnitus remains obscure because the cause and mechanism is poorly understood. In the absence of a suitable animal model, past investigations tool place in humans. Recently, the animal model with sodium salicylate ototoxicity is broadly used because of its reversibility. Balyangtongum-Tang had been used clinically to treat tinnitus and other hearing problems. We investigated the effects of Balyangtongum-Tang on cochlear morphologic change induced by sodium salicylate ototoxicity and were to find out its therapeutic effects on ototoxicity in rat model. Materials and Methods : Healthy ten Sprague-Dawley rats were divided into normal(2), control(4) and sample(4) groups. The sample group was given extract of Balyangtongum - Tang(1cc/100g) once a day for 4 days. After 3 hours when last medication were given, the sample and control groups were injected intraperitoneally with sodium salicylate(500mg/kg). We observed the cochlear morphologic changes of rats every 1, 2, 3 and 5 hours after injection. Results : The electron microscopic finding of outer hair cell shows some changes in the curticular plate and cytoplasm. Some vacuoles were found in the control and sample groups. Vacuolization in the curticular plate and cytoplasm of the sample group after 3, 5 hours were similar to the control group. But the curticular plate of the sample group after 1, 2 hours did not from vacuole. On the other hand the control group after 1, 2 hours formed vacuoles in the curticular plate. Light microscopic findings of cochlear duct in control and sample groups didn't find any difference. Conclusion : The results suggest that extract of Balyangtongum-Tang reduces the morphologic changes induced by sodium salicylate ototoxicity and the effects are remarkable in frist 2 hours. However the effective times are different with previous studies, it seems to due to the difference of tolerance and sensitivity of laboratory animals.

• 폴리머
• /
• 제32권5호
• /
• pp.403-408
• /
• 2008

케라틴은 울, 머리카락, 손톱 등을 형성하는 섬유단백질의 주요성분으로 유용한 생체재료이다. 골수간염 줄기세포를 이용한 조직공학 적용을 위해 Poly(4-lactide-co-glycolide) (PLGA)에 함량별로 케라틴을 함유한 지지체를 용매 캐스팅/염 추출법을 이용하여 제조하였다. 제조된 지지체의 표면과 단면의 형태를 전자현미경(SEM)으로 관찰하고 특성분석을 위해 다공도, 표면 적심성, 물 흡수성, 그리고 열적성질을 분석하였다. 이 후 쥐에서 분리한 골수간엽줄기세포를 지지체에 파종하여 세포의 증식율을 (4,5-dimethylthiazol-2-yl)-2.5-diphenyl-tetrazolium bromide(MTT) 분석방법을 이용하여 측정하였다. 천연/합성 하이브리드 담체인 케라틴/PLGA 지지체는 PLGA 단독으로 제조된 지지체와 비교 시 골수간엽줄기세포의 생장에 유익한 환경을 제공함을 확인하였다.